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The map label for this gene is argG

Identifier: 49187522

GI number: 49187522

Start: 4435545

End: 4436750

Strand: Reverse

Name: argG

Synonym: BAS4528

Alternate gene names: 49187522

Gene position: 4436750-4435545 (Counterclockwise)

Preceding gene: 49187524

Following gene: 49187521

Centisome position: 84.85

GC content: 37.89

Gene sequence:

>1206_bases
ATGGAGAAGAAAAAAGTTGTATTAGCATATTCCGGAGGTCTTGATACTTCCGTTGCAATTAAATGGTTACAAGAGAAGAA
TTATGATATTATCGCGCTTTGTTTAGACTTAGGGGAAGGTAAAGACTTAGCATTTGTAAAAGAAAAGGCACTTTCAGTAG
GTGCAATTAAATCATATATGATTGATGTTCAAGAAGAATTTGCAAACGAATATGCATTGATGGCGATGCAAGCACACACG
TTATACGAAGGGAAATATCCTCTCGTTTCGGCGTTATCTCGTCCACTTATCGCAAAGAAATTAGTAGAAATTGCAGAACA
AGAAGGAGCGACTGCAGTTGCACACGGATGTACAGGGAAAGGGAATGATCAAGTTCGTTTTGAAGTTTCTATTCAAGCAT
TAAATCCTTACTTAGAAGTAATTGCACCTGTACGTGAATGGAAATGGTCACGTGAAGAAGAAATTGCCTATGCAAAAGAA
AATAATGTACCGATTCCAATTAATTTAGATAGCCCGTTTTCAATCGATCAAAATTTATGGGGACGCAGCAATGAATGCGG
CATTTTAGAAGATCCATGGGCAGCGCCGCCAGAAGATGCATATGAGATGACATTGGCATTAGAAGACACACCGAATAAAC
CAGAATTTGTAGAAATTGGCTTTGAAGCAGGCGTACCGACTACTTTAAATGGCACTGCATATCCACTTTCAGAACTTATT
AAAACGTTAAATGCACTTGCTGGAAAACATGGCGTTGGACGTATCGATCACGTAGAAAATCGTCTTGTTGGTATTAAATC
ACGTGAAGTATACGAATGCCCAGCAGCAATGACATTAATTACGGCGCATAAAGAACTTGAAGATTTAACACTTGTAAAAG
AAGTAGCACATTTTAAACCGATGATTGAGCAAAAAATAACAGAGCTTATTTATAACGGTTTATGGTTCTCGCCTTTAAAA
CAAGCGCTTCATGCTTTCTTACAAGAAACGCAAAAGAATGTAACAGGCACGGTGCGTGTGAAATTATTTAAAGGTCATGC
AATTGTAGAAGGGCGTAAATCTGAGTACTCTTTATACGATGAAAAATTAGCAACGTATACTGCTCAGGATGAATTTAATC
ATGATGCAGCAGTTGGATTCATTTCATTATTCGGTTTACCGACGAAAGTATATAGCCAAGTGAATCAAAAGAAGGTGGAA
GCGTGA

Upstream 100 bases:

>100_bases
GAAAAAATTTGAAAATGGCTCTTGATACATTTTGTAAAAGGTGTTAAAGTGTGAATTATTATAAAAGAAAATGAATATTT
ATTCTTTAGGGGGATGCAAA

Downstream 100 bases:

>100_bases
GTAAACTTTGGGGCGGACGTTTTACGGAAGAAGCGGAAGCGTGGGTTGAAGAGTTCGGGGCATCCATCTCCTTCGATCAA
CAATTAGTAAATCAAGATAT

Product: argininosuccinate synthase

Products: NA

Alternate protein names: Citrulline--aspartate ligase

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MEKKKVVLAYSGGLDTSVAIKWLQEKNYDIIALCLDLGEGKDLAFVKEKALSVGAIKSYMIDVQEEFANEYALMAMQAHT
LYEGKYPLVSALSRPLIAKKLVEIAEQEGATAVAHGCTGKGNDQVRFEVSIQALNPYLEVIAPVREWKWSREEEIAYAKE
NNVPIPINLDSPFSIDQNLWGRSNECGILEDPWAAPPEDAYEMTLALEDTPNKPEFVEIGFEAGVPTTLNGTAYPLSELI
KTLNALAGKHGVGRIDHVENRLVGIKSREVYECPAAMTLITAHKELEDLTLVKEVAHFKPMIEQKITELIYNGLWFSPLK
QALHAFLQETQKNVTGTVRVKLFKGHAIVEGRKSEYSLYDEKLATYTAQDEFNHDAAVGFISLFGLPTKVYSQVNQKKVE
A

Sequences:

>Translated_401_residues
MEKKKVVLAYSGGLDTSVAIKWLQEKNYDIIALCLDLGEGKDLAFVKEKALSVGAIKSYMIDVQEEFANEYALMAMQAHT
LYEGKYPLVSALSRPLIAKKLVEIAEQEGATAVAHGCTGKGNDQVRFEVSIQALNPYLEVIAPVREWKWSREEEIAYAKE
NNVPIPINLDSPFSIDQNLWGRSNECGILEDPWAAPPEDAYEMTLALEDTPNKPEFVEIGFEAGVPTTLNGTAYPLSELI
KTLNALAGKHGVGRIDHVENRLVGIKSREVYECPAAMTLITAHKELEDLTLVKEVAHFKPMIEQKITELIYNGLWFSPLK
QALHAFLQETQKNVTGTVRVKLFKGHAIVEGRKSEYSLYDEKLATYTAQDEFNHDAAVGFISLFGLPTKVYSQVNQKKVE
A
>Mature_401_residues
MEKKKVVLAYSGGLDTSVAIKWLQEKNYDIIALCLDLGEGKDLAFVKEKALSVGAIKSYMIDVQEEFANEYALMAMQAHT
LYEGKYPLVSALSRPLIAKKLVEIAEQEGATAVAHGCTGKGNDQVRFEVSIQALNPYLEVIAPVREWKWSREEEIAYAKE
NNVPIPINLDSPFSIDQNLWGRSNECGILEDPWAAPPEDAYEMTLALEDTPNKPEFVEIGFEAGVPTTLNGTAYPLSELI
KTLNALAGKHGVGRIDHVENRLVGIKSREVYECPAAMTLITAHKELEDLTLVKEVAHFKPMIEQKITELIYNGLWFSPLK
QALHAFLQETQKNVTGTVRVKLFKGHAIVEGRKSEYSLYDEKLATYTAQDEFNHDAAVGFISLFGLPTKVYSQVNQKKVE
A

Specific function: Arginine biosynthesis; seventh step. [C]

COG id: COG0137

COG function: function code E; Argininosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the argininosuccinate synthase family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI53759107, Length=409, Percent_Identity=43.520782396088, Blast_Score=311, Evalue=6e-85,
Organism=Homo sapiens, GI16950633, Length=409, Percent_Identity=43.520782396088, Blast_Score=311, Evalue=6e-85,
Organism=Escherichia coli, GI1789563, Length=406, Percent_Identity=26.6009852216749, Blast_Score=117, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324514, Length=417, Percent_Identity=38.6091127098321, Blast_Score=284, Evalue=2e-77,
Organism=Drosophila melanogaster, GI21358151, Length=398, Percent_Identity=39.6984924623116, Blast_Score=285, Evalue=3e-77,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): ASSY_BACAA (C3PB97)

Other databases:

- EMBL:   CP001598
- RefSeq:   YP_002868913.1
- ProteinModelPortal:   C3PB97
- SMR:   C3PB97
- EnsemblBacteria:   EBBACT00000126392
- GeneID:   7848004
- GenomeReviews:   CP001598_GR
- KEGG:   bai:BAA_4891
- GeneTree:   EBGT00050000002857
- ProtClustDB:   PRK00509
- GO:   GO:0005737
- HAMAP:   MF_00005
- InterPro:   IPR001518
- InterPro:   IPR018223
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR11587
- TIGRFAMs:   TIGR00032

Pfam domain/function: PF00764 Arginosuc_synth

EC number: =6.3.4.5

Molecular weight: Translated: 44694; Mature: 44694

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00564 ARGININOSUCCIN_SYN_1; PS00565 ARGININOSUCCIN_SYN_2

Important sites: BINDING 86-86 BINDING 116-116 BINDING 118-118 BINDING 122-122 BINDING 122-122 BINDING 123-123 BINDING 126-126 BINDING 174-174 BINDING 183-183 BINDING 259-259 BINDING 271-271

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKKVVLAYSGGLDTSVAIKWLQEKNYDIIALCLDLGEGKDLAFVKEKALSVGAIKSYM
CCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
IDVQEEFANEYALMAMQAHTLYEGKYPLVSALSRPLIAKKLVEIAEQEGATAVAHGCTGK
HHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHCCCEEEEECCCCC
GNDQVRFEVSIQALNPYLEVIAPVREWKWSREEEIAYAKENNVPIPINLDSPFSIDQNLW
CCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCCHHCEEECCCCCEEEEECCCCCCCCHHHC
GRSNECGILEDPWAAPPEDAYEMTLALEDTPNKPEFVEIGFEAGVPTTLNGTAYPLSELI
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHH
KTLNALAGKHGVGRIDHVENRLVGIKSREVYECPAAMTLITAHKELEDLTLVKEVAHFKP
HHHHHHHCCCCCCCHHHHHHHEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MIEQKITELIYNGLWFSPLKQALHAFLQETQKNVTGTVRVKLFKGHAIVEGRKSEYSLYD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEECCCCCCHHHHH
EKLATYTAQDEFNHDAAVGFISLFGLPTKVYSQVNQKKVEA
HHHHHEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MEKKKVVLAYSGGLDTSVAIKWLQEKNYDIIALCLDLGEGKDLAFVKEKALSVGAIKSYM
CCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
IDVQEEFANEYALMAMQAHTLYEGKYPLVSALSRPLIAKKLVEIAEQEGATAVAHGCTGK
HHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHHHHHHCCCEEEEECCCCC
GNDQVRFEVSIQALNPYLEVIAPVREWKWSREEEIAYAKENNVPIPINLDSPFSIDQNLW
CCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCCHHCEEECCCCCEEEEECCCCCCCCHHHC
GRSNECGILEDPWAAPPEDAYEMTLALEDTPNKPEFVEIGFEAGVPTTLNGTAYPLSELI
CCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCCCCCCCHHHHH
KTLNALAGKHGVGRIDHVENRLVGIKSREVYECPAAMTLITAHKELEDLTLVKEVAHFKP
HHHHHHHCCCCCCCHHHHHHHEECCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
MIEQKITELIYNGLWFSPLKQALHAFLQETQKNVTGTVRVKLFKGHAIVEGRKSEYSLYD
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEECCCCCCHHHHH
EKLATYTAQDEFNHDAAVGFISLFGLPTKVYSQVNQKKVEA
HHHHHEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA