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The map label for this gene is yhcA [H]

Identifier: 49187459

GI number: 49187459

Start: 4375171

End: 4376970

Strand: Reverse

Name: yhcA [H]

Synonym: BAS4464

Alternate gene names: 49187459

Gene position: 4376970-4375171 (Counterclockwise)

Preceding gene: 49187460

Following gene: 49187452

Centisome position: 83.71

GC content: 37.94

Gene sequence:

>1800_bases
ATGTCCTCAATCGTAATTGCAGGATATGCACTATTTTGTATAATCGTCTTCTTCCTTGTAAATCGTCTTTTACGAAAGAA
AAAAACGAAAATGGGAGAAGAACAAGTCCCAGCCGTAAGTAAAATAGAAGTAAGTAAAGTTGAAACAGAAGAGAAAGACA
TTGAACACAAGCAAGAACCGGAAATTTCTAATGTAGTTAGGTTAGAAACAGGAAAGCAAGAAGAAGTAAAACAAGAAAAA
GAAGTGCCGAAAAAACAAATGTCTACGCCTGTAGAGAATGTGAACGTAAAAGCAGTTGTAGCTGTATTAATTCTCGGCAT
GTTCGTTTCTATTTTAAACCAAACAATCATTAACGTTGCATTACCTCCGTTAATGAATGAATTTAACGTATCAACTTCAA
CAGCACAATGGTTAATTACAGGTTTCATGCTTGTAAATGGTATTTTAGTACCGATTAGTGCCTTTTTAGTTTCACGGTTT
ACGTATCGTAAATTATTCGTAGCCGCAATGTTATTCTTTACGGTAGGATCTATCATTTGTGCTACTTCAGGAAACTTTAC
AATGATGATGACAGGCCGCGTAATTCAAGCGGTTGGTGCGGGTATTTTAATGCCGGTTGGTATGAATATCTTCATGACGT
TGTTCCCGCCTCATAAACGCGGAGCAGCGATGGGATTATTAGGTGTAGCGATGATTTTAGCTCCAGCTATCGGACCAACT
GTAACAGGTTGGGTGATTGAAAACTATAGCTGGAACTTAATGTTCTATGCGATGTTTATTATTGGTTTAATTATTACGTT
CCTGTCTTTAAAATTCTTTACACTAGCTCAGCCAGTGTCTAACACGAAATTAGATATATTCGGTGTGGTTAGTTCAAGTA
TTGGACTAGGAAGCTTATTGTATGGATTTAGTGAGGCTGGAAACAATAGTTGGACTAGTGCAGAAGTCATTATTTCACTT
GTGATCGGTGTAATTGGTTTAGCGTTATTTATTTGGAGAGAGTTAACAACGGACAATAAAATGCTAGATTTACAAGTGTT
TAAATACCCGGTATTTACATTTACTTTAGTAATTAACGCAATCGTTACGATGGCATTATTCGGAGGTATGTTATTGCTTC
CGGTATACCTGCAAAACATTCGTGGCTTCACGCCAATTGAATCTGGTTTACTACTTCTTCCAGGATCATTAATTATGGGG
ATCATGGGACCAGTAGCGGGTAAATTATTCGATAAATATGGCATTCGTCCATTAGCTATAATTGGATTGGCAATTACGAC
GTATGCGACATATGAATTTACAAAATTATCGATGGATACACCGTATAGCGTTATTATGACGGATTATATTATACGCTCAA
TTGGTATGTCATTCATTATGATGCCAATTATGACAGCTGGTATGAACGCGCTACCGATGAAATTAATTTCTCACGGTACA
GCAACGCAAAATACGTCAAGACAAGTAGCTGGTTCAATTGGAACAGCGATATTAATCACGCTTATGACGCAACAAACGAC
TGCTCACGTAGCAGATTATGGAAACATGTTAACAACGTCAAATCCGATTTTAGTTGATAAAGTGCATGGCATGGGGCAAA
GCTTAGCCGCCTTAGCTGGCTCAGCGCAAGCAGGAGATGCGATGAGCACGCAGCTATTATTTGGACAAATTTCGAAGTTA
TCTGCAATTAACGGTATTAACGATGCTTTCTTAATTGCGACGATACTCGCAGGGATTGCTTGGGTGTTATCGTTCTTCTT
ACCATCAGGCAATAAAGCAAATAGAAAAGCAGGGAATTAA

Upstream 100 bases:

>100_bases
AGTAGTACCAGTTAAAATTTCTTTAGATCATAGCAAATCAGTAAATATTGTTCCTGGCATGAATGTGACAGTTCGTATTC
ATAAGTAAGGGGGAGATGAG

Downstream 100 bases:

>100_bases
TTTCCCTGCTTTTTTGTGCTTTTTATGCAGTTACATATAAAAGATGATAACCAGTTACGGAGCAAAGCCCTGCTATAAAC
AAAATCGTCCCCATAGAACG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 599; Mature: 598

Protein sequence:

>599_residues
MSSIVIAGYALFCIIVFFLVNRLLRKKKTKMGEEQVPAVSKIEVSKVETEEKDIEHKQEPEISNVVRLETGKQEEVKQEK
EVPKKQMSTPVENVNVKAVVAVLILGMFVSILNQTIINVALPPLMNEFNVSTSTAQWLITGFMLVNGILVPISAFLVSRF
TYRKLFVAAMLFFTVGSIICATSGNFTMMMTGRVIQAVGAGILMPVGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPT
VTGWVIENYSWNLMFYAMFIIGLIITFLSLKFFTLAQPVSNTKLDIFGVVSSSIGLGSLLYGFSEAGNNSWTSAEVIISL
VIGVIGLALFIWRELTTDNKMLDLQVFKYPVFTFTLVINAIVTMALFGGMLLLPVYLQNIRGFTPIESGLLLLPGSLIMG
IMGPVAGKLFDKYGIRPLAIIGLAITTYATYEFTKLSMDTPYSVIMTDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGT
ATQNTSRQVAGSIGTAILITLMTQQTTAHVADYGNMLTTSNPILVDKVHGMGQSLAALAGSAQAGDAMSTQLLFGQISKL
SAINGINDAFLIATILAGIAWVLSFFLPSGNKANRKAGN

Sequences:

>Translated_599_residues
MSSIVIAGYALFCIIVFFLVNRLLRKKKTKMGEEQVPAVSKIEVSKVETEEKDIEHKQEPEISNVVRLETGKQEEVKQEK
EVPKKQMSTPVENVNVKAVVAVLILGMFVSILNQTIINVALPPLMNEFNVSTSTAQWLITGFMLVNGILVPISAFLVSRF
TYRKLFVAAMLFFTVGSIICATSGNFTMMMTGRVIQAVGAGILMPVGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPT
VTGWVIENYSWNLMFYAMFIIGLIITFLSLKFFTLAQPVSNTKLDIFGVVSSSIGLGSLLYGFSEAGNNSWTSAEVIISL
VIGVIGLALFIWRELTTDNKMLDLQVFKYPVFTFTLVINAIVTMALFGGMLLLPVYLQNIRGFTPIESGLLLLPGSLIMG
IMGPVAGKLFDKYGIRPLAIIGLAITTYATYEFTKLSMDTPYSVIMTDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGT
ATQNTSRQVAGSIGTAILITLMTQQTTAHVADYGNMLTTSNPILVDKVHGMGQSLAALAGSAQAGDAMSTQLLFGQISKL
SAINGINDAFLIATILAGIAWVLSFFLPSGNKANRKAGN
>Mature_598_residues
SSIVIAGYALFCIIVFFLVNRLLRKKKTKMGEEQVPAVSKIEVSKVETEEKDIEHKQEPEISNVVRLETGKQEEVKQEKE
VPKKQMSTPVENVNVKAVVAVLILGMFVSILNQTIINVALPPLMNEFNVSTSTAQWLITGFMLVNGILVPISAFLVSRFT
YRKLFVAAMLFFTVGSIICATSGNFTMMMTGRVIQAVGAGILMPVGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPTV
TGWVIENYSWNLMFYAMFIIGLIITFLSLKFFTLAQPVSNTKLDIFGVVSSSIGLGSLLYGFSEAGNNSWTSAEVIISLV
IGVIGLALFIWRELTTDNKMLDLQVFKYPVFTFTLVINAIVTMALFGGMLLLPVYLQNIRGFTPIESGLLLLPGSLIMGI
MGPVAGKLFDKYGIRPLAIIGLAITTYATYEFTKLSMDTPYSVIMTDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGTA
TQNTSRQVAGSIGTAILITLMTQQTTAHVADYGNMLTTSNPILVDKVHGMGQSLAALAGSAQAGDAMSTQLLFGQISKLS
AINGINDAFLIATILAGIAWVLSFFLPSGNKANRKAGN

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=505, Percent_Identity=28.5148514851485, Blast_Score=207, Evalue=2e-54,
Organism=Escherichia coli, GI1788710, Length=504, Percent_Identity=26.5873015873016, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI87081983, Length=407, Percent_Identity=25.0614250614251, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1790195, Length=338, Percent_Identity=26.9230769230769, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1788392, Length=422, Percent_Identity=24.8815165876777, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322958, Length=374, Percent_Identity=27.2727272727273, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=285, Percent_Identity=24.2105263157895, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=318, Percent_Identity=24.8427672955975, Blast_Score=68, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 64871; Mature: 64740

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSIVIAGYALFCIIVFFLVNRLLRKKKTKMGEEQVPAVSKIEVSKVETEEKDIEHKQEP
CCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHEEHHHHHCHHHHCCCCCCC
EISNVVRLETGKQEEVKQEKEVPKKQMSTPVENVNVKAVVAVLILGMFVSILNQTIINVA
CHHCEEEECCCCHHHHHHHHCCCHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LPPLMNEFNVSTSTAQWLITGFMLVNGILVPISAFLVSRFTYRKLFVAAMLFFTVGSIIC
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
ATSGNFTMMMTGRVIQAVGAGILMPVGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPT
ECCCCEEEEEHHHHHHHHCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
VTGWVIENYSWNLMFYAMFIIGLIITFLSLKFFTLAQPVSNTKLDIFGVVSSSIGLGSLL
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCHHHHH
YGFSEAGNNSWTSAEVIISLVIGVIGLALFIWRELTTDNKMLDLQVFKYPVFTFTLVINA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
IVTMALFGGMLLLPVYLQNIRGFTPIESGLLLLPGSLIMGIMGPVAGKLFDKYGIRPLAI
HHHHHHHHHHHHHHHHHHHCCCCCCHHCCCEECCHHHHHHHHHHHHHHHHHHHCCCHHHH
IGLAITTYATYEFTKLSMDTPYSVIMTDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGT
HHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
ATQNTSRQVAGSIGTAILITLMTQQTTAHVADYGNMLTTSNPILVDKVHGMGQSLAALAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECHHCCCHHHHHHHC
SAQAGDAMSTQLLFGQISKLSAINGINDAFLIATILAGIAWVLSFFLPSGNKANRKAGN
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SSIVIAGYALFCIIVFFLVNRLLRKKKTKMGEEQVPAVSKIEVSKVETEEKDIEHKQEP
CCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHEEHHHHHCHHHHCCCCCCC
EISNVVRLETGKQEEVKQEKEVPKKQMSTPVENVNVKAVVAVLILGMFVSILNQTIINVA
CHHCEEEECCCCHHHHHHHHCCCHHHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LPPLMNEFNVSTSTAQWLITGFMLVNGILVPISAFLVSRFTYRKLFVAAMLFFTVGSIIC
CCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
ATSGNFTMMMTGRVIQAVGAGILMPVGMNIFMTLFPPHKRGAAMGLLGVAMILAPAIGPT
ECCCCEEEEEHHHHHHHHCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHHHCCCCC
VTGWVIENYSWNLMFYAMFIIGLIITFLSLKFFTLAQPVSNTKLDIFGVVSSSIGLGSLL
EEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCHHHHH
YGFSEAGNNSWTSAEVIISLVIGVIGLALFIWRELTTDNKMLDLQVFKYPVFTFTLVINA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
IVTMALFGGMLLLPVYLQNIRGFTPIESGLLLLPGSLIMGIMGPVAGKLFDKYGIRPLAI
HHHHHHHHHHHHHHHHHHHCCCCCCHHCCCEECCHHHHHHHHHHHHHHHHHHHCCCHHHH
IGLAITTYATYEFTKLSMDTPYSVIMTDYIIRSIGMSFIMMPIMTAGMNALPMKLISHGT
HHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCC
ATQNTSRQVAGSIGTAILITLMTQQTTAHVADYGNMLTTSNPILVDKVHGMGQSLAALAG
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECHHCCCHHHHHHHC
SAQAGDAMSTQLLFGQISKLSAINGINDAFLIATILAGIAWVLSFFLPSGNKANRKAGN
CCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377; 8969498 [H]