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The map label for this gene is nadB [H]

Identifier: 49187322

GI number: 49187322

Start: 4237819

End: 4239348

Strand: Reverse

Name: nadB [H]

Synonym: BAS4327

Alternate gene names: 49187322

Gene position: 4239348-4237819 (Counterclockwise)

Preceding gene: 49187326

Following gene: 49187321

Centisome position: 81.08

GC content: 39.15

Gene sequence:

>1530_bases
ATGCCAAGTGCAGATGTCTTAATTATTGGAAGTGGTATTGCGGCACTTCGTGTTGCAAAAGAGATTTGCCACGAAAAGAA
TGTGATTATTATCACAAAGGAAACGAATCGTAATAATAATACACATTTAGCGCAAGGTGGAATTGCCGCAGCGGTTGCTA
CATATGACAATCCGAATGATCATTTTGAAGATACGTTAGTAGCAGGATGTCGTTATAACAATGAAGATGTGGTCCGTTAT
TTAGTTGAAGAAGGACCGAAAGAAATGAAGAATCTCATTGAAAATGGAATGCAATTTGATGGAGATGAAACGGGTCCCCA
TCTTGGAAAAGAAGGCGCACATCGAAAGCGTCGCATTTTACATGCAGGTGGCGATGCAACAGGAAAGAATTTATTAGAGC
ATTTCATTCAAGAAGTAGTTCCGCATGTTACGGTAGTGGAACAGGAAATGGTGCTTGATTTTATTATAGAAAGTGAAAAA
TGTGTTGGAGTTTTAACGCGAAATAGTGAAGGGAAGTTGAAGCGTTATGATGCAGATTATACTGTGCTAGCCTCAGGCGG
GGTTGGTGGTTTATACGCCTTTACTTCTAATGATAAAACAATTACGGGTGATGGACTTGCGATGGTATACCGCGCAGGCG
GCGAGCTCGTAGATTTAGAGTTTGTACAATTTCATCCAACAATGTTATATGCAGGCGGGCAATGCTGTGGCCTCGTTTCT
GAAGCTGTCCGTGGTGAAGGAGCTGTCCTTATAAATGGAAAAGGGCAGCGTTTTATGATGGATATACACGATGATTACGA
TCTCGCACCGCGTGATGTGGTGGCAAGAGCGATTCATGAACAGCTTTTGTCGGGTGAAAAAGTGTATTTGAATATTGGGT
CTATCCAAAATTTCCAAGAACGTTTTCCAACTGTATCAGCATTGTGTAAAAAGAATGGTGTTGATATTACTAAAAAACGC
ATTCCGGTCGTACCCGGAGCTCATTTTCATATGGGCGGTGTGAAAACAAACATTGATGGGGAGACGTCTATTCCAAATTT
ATATGCAGTCGGTGAAGTAGCTTGTAATGGTGTTCATGGAGCAAATAGATTAGCGAGTAATTCGTTATTAGAAGGTCTTG
TGTTTGGAAAAAGAATAGGACAACATATTTTAACGAAAGCGACAAAAGACAAATTGAACGACTTCGCTGAGAAGGAAAAG
AAATTTATCGTTCTAAATCATTTGCCGACGAAAGAAGAAATACAAGAATGCATGATGAAATATGTTGGGATTGTACGAAC
AGAGCAAAGTTTATCTTATGCGAAGAGATGGCTTAGTAAATACGGTGTTCGAAATATGATATTACAACATGATGCTCTTA
CAAATGAAGAGATAACGCTTATTAATATGTTAACAGTTTGTGAGCTTATCGTTGTATCAGCTTTACAAAGAGAAGAGAGT
ATTGGTGGTCATTATCGAAGTGATTATCCACATAGAAATAGCGCACAAAAAGAGATCATTCGAGTGAAAAGAAAACTACA
ACTTGTGTAA

Upstream 100 bases:

>100_bases
GTGAAAATAAGAATTATAAAAAAACTCTTGTCATTATTTTAAAACTATGTAAATATAGGTGTCAAGACAGTTGAAAAGTG
TAAAGTAGGAGGCAACAATT

Downstream 100 bases:

>100_bases
TGAGGGGAAGAGGGGCTTTATGAATAGGATAAAAGTGAAAGAAGCATTAAATCGATTTTTTCTAGAAGATATAGGAGAAA
GAGATGTAACATCTCAGCTT

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MPSADVLIIGSGIAALRVAKEICHEKNVIIITKETNRNNNTHLAQGGIAAAVATYDNPNDHFEDTLVAGCRYNNEDVVRY
LVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRILHAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEK
CVGVLTRNSEGKLKRYDADYTVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGQCCGLVS
EAVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIGSIQNFQERFPTVSALCKKNGVDITKKR
IPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHGANRLASNSLLEGLVFGKRIGQHILTKATKDKLNDFAEKEK
KFIVLNHLPTKEEIQECMMKYVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
IGGHYRSDYPHRNSAQKEIIRVKRKLQLV

Sequences:

>Translated_509_residues
MPSADVLIIGSGIAALRVAKEICHEKNVIIITKETNRNNNTHLAQGGIAAAVATYDNPNDHFEDTLVAGCRYNNEDVVRY
LVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRILHAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEK
CVGVLTRNSEGKLKRYDADYTVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGQCCGLVS
EAVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIGSIQNFQERFPTVSALCKKNGVDITKKR
IPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHGANRLASNSLLEGLVFGKRIGQHILTKATKDKLNDFAEKEK
KFIVLNHLPTKEEIQECMMKYVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
IGGHYRSDYPHRNSAQKEIIRVKRKLQLV
>Mature_508_residues
PSADVLIIGSGIAALRVAKEICHEKNVIIITKETNRNNNTHLAQGGIAAAVATYDNPNDHFEDTLVAGCRYNNEDVVRYL
VEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRILHAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEKC
VGVLTRNSEGKLKRYDADYTVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGQCCGLVSE
AVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIGSIQNFQERFPTVSALCKKNGVDITKKRI
PVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHGANRLASNSLLEGLVFGKRIGQHILTKATKDKLNDFAEKEKK
FIVLNHLPTKEEIQECMMKYVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREESI
GGHYRSDYPHRNSAQKEIIRVKRKLQLV

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=524, Percent_Identity=33.7786259541985, Blast_Score=216, Evalue=4e-56,
Organism=Escherichia coli, GI1788928, Length=517, Percent_Identity=36.7504835589942, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1790597, Length=511, Percent_Identity=32.0939334637965, Blast_Score=205, Evalue=4e-54,
Organism=Escherichia coli, GI1786942, Length=522, Percent_Identity=30.6513409961686, Blast_Score=187, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17550100, Length=522, Percent_Identity=31.992337164751, Blast_Score=213, Evalue=2e-55,
Organism=Caenorhabditis elegans, GI17505833, Length=524, Percent_Identity=31.8702290076336, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI71986328, Length=462, Percent_Identity=25.7575757575758, Blast_Score=95, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6322701, Length=528, Percent_Identity=34.280303030303, Blast_Score=233, Evalue=4e-62,
Organism=Saccharomyces cerevisiae, GI6322416, Length=515, Percent_Identity=33.7864077669903, Blast_Score=220, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6320788, Length=479, Percent_Identity=26.0960334029228, Blast_Score=100, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6322511, Length=473, Percent_Identity=25.369978858351, Blast_Score=88, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17137288, Length=522, Percent_Identity=34.2911877394636, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24655642, Length=522, Percent_Identity=34.2911877394636, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24655647, Length=522, Percent_Identity=34.2911877394636, Blast_Score=217, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24663005, Length=523, Percent_Identity=31.1663479923518, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 56278; Mature: 56147

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00079 MULTICOPPER_OXIDASE1 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSADVLIIGSGIAALRVAKEICHEKNVIIITKETNRNNNTHLAQGGIAAAVATYDNPND
CCCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCEEEEEEECCCCCC
HFEDTLVAGCRYNNEDVVRYLVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRIL
HHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHE
HAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEKCVGVLTRNSEGKLKRYDADY
ECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCE
TVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGQCCGLVS
EEEECCCCCEEEEEECCCCEECCCCEEEEEECCCCEEEEEEEEECCEEEECCCHHHHHHH
EAVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIGSIQNFQE
HHHCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
RFPTVSALCKKNGVDITKKRIPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHG
HCCHHHHHHHCCCCCCHHHCCCCCCCCCEECCCEEECCCCCCCCCCEEEEHHHHHCCCCC
ANRLASNSLLEGLVFGKRIGQHILTKATKDKLNDFAEKEKKFIVLNHLPTKEEIQECMMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHH
YVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
HHHHHCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGGHYRSDYPHRNSAQKEIIRVKRKLQLV
CCCCCCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PSADVLIIGSGIAALRVAKEICHEKNVIIITKETNRNNNTHLAQGGIAAAVATYDNPND
CCCCEEEEECCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCEEEEEEECCCCCC
HFEDTLVAGCRYNNEDVVRYLVEEGPKEMKNLIENGMQFDGDETGPHLGKEGAHRKRRIL
HHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHE
HAGGDATGKNLLEHFIQEVVPHVTVVEQEMVLDFIIESEKCVGVLTRNSEGKLKRYDADY
ECCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCE
TVLASGGVGGLYAFTSNDKTITGDGLAMVYRAGGELVDLEFVQFHPTMLYAGGQCCGLVS
EEEECCCCCEEEEEECCCCEECCCCEEEEEECCCCEEEEEEEEECCEEEECCCHHHHHHH
EAVRGEGAVLINGKGQRFMMDIHDDYDLAPRDVVARAIHEQLLSGEKVYLNIGSIQNFQE
HHHCCCCEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHH
RFPTVSALCKKNGVDITKKRIPVVPGAHFHMGGVKTNIDGETSIPNLYAVGEVACNGVHG
HCCHHHHHHHCCCCCCHHHCCCCCCCCCEECCCEEECCCCCCCCCCEEEEHHHHHCCCCC
ANRLASNSLLEGLVFGKRIGQHILTKATKDKLNDFAEKEKKFIVLNHLPTKEEIQECMMK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHH
YVGIVRTEQSLSYAKRWLSKYGVRNMILQHDALTNEEITLINMLTVCELIVVSALQREES
HHHHHCCHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGGHYRSDYPHRNSAQKEIIRVKRKLQLV
CCCCCCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8444804; 9384377 [H]