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The map label for this gene is iscS1 [H]

Identifier: 49187288

GI number: 49187288

Start: 4202812

End: 4203957

Strand: Reverse

Name: iscS1 [H]

Synonym: BAS4292

Alternate gene names: 49187288

Gene position: 4203957-4202812 (Counterclockwise)

Preceding gene: 49187289

Following gene: 49187287

Centisome position: 80.4

GC content: 39.79

Gene sequence:

>1146_bases
ATGGAACGCATTTACTTAGATCATGCTGCGACATCTCCGACTCACCTAGAAGTGGTCGAAAAGATGATTCCATATATGAC
AGAAACATTCGGAAACCCGTCTAGTATTCACTTTTATGGACGTCAAACGCGCCATGCGGTAGATGAGGCGAGACGCGCGT
GTGCACGTAGCATTCATGCGAATCCGAATGAAATTATATTTACGAGCGGTGGTACAGAAGCTGATAATTTAGCACTTATC
GGTGTAGCGCGTGCGAACCGTCATAAAGGTAACCATATTATAACGACGCAAATTGAACATCATGCAATTTTGCATACGTG
TGAATTGTTAGAGCGTGAAGGGTTTGAAGTGACATATTTACCTGTTGATGAAACAGGACGCGTTCAAGTTTCTGACATAC
AAAAGGCATTGACAGAAGAGACGATTCTTGTATCTGTTATGTTTGGGAATAATGAAGTTGGGACGATGCAACCAATTGCA
GAAATAGGTAAGCTGCTAAAAGAGCATCAAGCTTATTTCCATACAGATGCGGTACAAGCTTACGGTTTAGTAGAAATTAA
TGTGAAAGAGTTTGGTATTGATTTATTATCGATTTCAGCTCATAAAATTAACGGACCAAAAGGTGTAGGGTTCTTATACG
CTGGTACAAATGTGAAATTTGAACCGTTATTAATAGGCGGAGAGCAGGAAAGAAAACGCCGTGCTGGTACTGAAAATGTG
CCAAGTATTGTTGGTTTACAACATGCAATTTTGCTAGCTGAAAAAACTCGTGAGCAAAAAAATGCCCAATATGAAGAGTT
TAAAGATATAATGGTATCTGTCTTCCAAAATGAAGACATTACTTTCGAGGTAAACGGAAACTTAGAATATCGCTTACCAC
ATGTGCTTAATATTAGTTTTACAGGAATGAACATTGAACCGTTTCTTGTGAACTTAGACTTAGCTGGCATTGCTGTATCA
AGTGGTTCGGCGTGTACAGCTGGTTCTATTGATCCATCACATGTATTAGTGGCGATGTTCGGAAAAGACTCCGATCAAAT
ACGTTCATCCGTACGCTTTAGTTTCGGACTTGGAAATACGAAAGAGCAAATTGAGAAAGCGGCGTACGAAACAGTGAAAA
TCGTGAAGCGATTAACACAAAATTAG

Upstream 100 bases:

>100_bases
CAACGTTAGAAGATTTAGTACGTTATGAGGAAGAAAATCACGGGGGCTATATGTTTTACATTTAATTCCTTGTGAAACGA
TTTGGAAAGAAGGAGATTTA

Downstream 100 bases:

>100_bases
CATGGGAGGTGACATGATGAACAAACTACCTCACGAAACACGCGTTGTCATCGGGATGTCCGGTGGCGTCGATTCATCTG
TAGCGGCTCTTTTATTAAAA

Product: class V aminotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MERIYLDHAATSPTHLEVVEKMIPYMTETFGNPSSIHFYGRQTRHAVDEARRACARSIHANPNEIIFTSGGTEADNLALI
GVARANRHKGNHIITTQIEHHAILHTCELLEREGFEVTYLPVDETGRVQVSDIQKALTEETILVSVMFGNNEVGTMQPIA
EIGKLLKEHQAYFHTDAVQAYGLVEINVKEFGIDLLSISAHKINGPKGVGFLYAGTNVKFEPLLIGGEQERKRRAGTENV
PSIVGLQHAILLAEKTREQKNAQYEEFKDIMVSVFQNEDITFEVNGNLEYRLPHVLNISFTGMNIEPFLVNLDLAGIAVS
SGSACTAGSIDPSHVLVAMFGKDSDQIRSSVRFSFGLGNTKEQIEKAAYETVKIVKRLTQN

Sequences:

>Translated_381_residues
MERIYLDHAATSPTHLEVVEKMIPYMTETFGNPSSIHFYGRQTRHAVDEARRACARSIHANPNEIIFTSGGTEADNLALI
GVARANRHKGNHIITTQIEHHAILHTCELLEREGFEVTYLPVDETGRVQVSDIQKALTEETILVSVMFGNNEVGTMQPIA
EIGKLLKEHQAYFHTDAVQAYGLVEINVKEFGIDLLSISAHKINGPKGVGFLYAGTNVKFEPLLIGGEQERKRRAGTENV
PSIVGLQHAILLAEKTREQKNAQYEEFKDIMVSVFQNEDITFEVNGNLEYRLPHVLNISFTGMNIEPFLVNLDLAGIAVS
SGSACTAGSIDPSHVLVAMFGKDSDQIRSSVRFSFGLGNTKEQIEKAAYETVKIVKRLTQN
>Mature_381_residues
MERIYLDHAATSPTHLEVVEKMIPYMTETFGNPSSIHFYGRQTRHAVDEARRACARSIHANPNEIIFTSGGTEADNLALI
GVARANRHKGNHIITTQIEHHAILHTCELLEREGFEVTYLPVDETGRVQVSDIQKALTEETILVSVMFGNNEVGTMQPIA
EIGKLLKEHQAYFHTDAVQAYGLVEINVKEFGIDLLSISAHKINGPKGVGFLYAGTNVKFEPLLIGGEQERKRRAGTENV
PSIVGLQHAILLAEKTREQKNAQYEEFKDIMVSVFQNEDITFEVNGNLEYRLPHVLNISFTGMNIEPFLVNLDLAGIAVS
SGSACTAGSIDPSHVLVAMFGKDSDQIRSSVRFSFGLGNTKEQIEKAAYETVKIVKRLTQN

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=380, Percent_Identity=41.8421052631579, Blast_Score=301, Evalue=7e-82,
Organism=Homo sapiens, GI156713448, Length=413, Percent_Identity=33.4140435835351, Blast_Score=202, Evalue=5e-52,
Organism=Escherichia coli, GI48994898, Length=387, Percent_Identity=42.3772609819121, Blast_Score=283, Evalue=9e-78,
Organism=Escherichia coli, GI1787970, Length=282, Percent_Identity=26.5957446808511, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1789175, Length=383, Percent_Identity=24.8041775456919, Blast_Score=89, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI25143064, Length=379, Percent_Identity=44.5910290237467, Blast_Score=308, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI17533177, Length=325, Percent_Identity=30.4615384615385, Blast_Score=147, Evalue=8e-36,
Organism=Saccharomyces cerevisiae, GI6319831, Length=382, Percent_Identity=43.717277486911, Blast_Score=304, Evalue=2e-83,
Organism=Drosophila melanogaster, GI20129463, Length=388, Percent_Identity=43.8144329896907, Blast_Score=295, Evalue=3e-80,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42244; Mature: 42244

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERIYLDHAATSPTHLEVVEKMIPYMTETFGNPSSIHFYGRQTRHAVDEARRACARSIHA
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCC
NPNEIIFTSGGTEADNLALIGVARANRHKGNHIITTQIEHHAILHTCELLEREGFEVTYL
CCCEEEEECCCCCCCCEEEEEEEECCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCEEEEE
PVDETGRVQVSDIQKALTEETILVSVMFGNNEVGTMQPIAEIGKLLKEHQAYFHTDAVQA
ECCCCCCEEHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHE
YGLVEINVKEFGIDLLSISAHKINGPKGVGFLYAGTNVKFEPLLIGGEQERKRRAGTENV
EEEEEEEHHHHCEEEEEEEEEECCCCCCCEEEEECCCEEEEEEEECCCHHHHHHCCCCCC
PSIVGLQHAILLAEKTREQKNAQYEEFKDIMVSVFQNEDITFEVNGNLEYRLPHVLNISF
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCEEEECCEEEEEEE
TGMNIEPFLVNLDLAGIAVSSGSACTAGSIDPSHVLVAMFGKDSDQIRSSVRFSFGLGNT
ECCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHEEEEECCCCC
KEQIEKAAYETVKIVKRLTQN
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MERIYLDHAATSPTHLEVVEKMIPYMTETFGNPSSIHFYGRQTRHAVDEARRACARSIHA
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHCC
NPNEIIFTSGGTEADNLALIGVARANRHKGNHIITTQIEHHAILHTCELLEREGFEVTYL
CCCEEEEECCCCCCCCEEEEEEEECCCCCCCEEEEEEEHHHHHHHHHHHHHHCCCEEEEE
PVDETGRVQVSDIQKALTEETILVSVMFGNNEVGTMQPIAEIGKLLKEHQAYFHTDAVQA
ECCCCCCEEHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHE
YGLVEINVKEFGIDLLSISAHKINGPKGVGFLYAGTNVKFEPLLIGGEQERKRRAGTENV
EEEEEEEHHHHCEEEEEEEEEECCCCCCCEEEEECCCEEEEEEEECCCHHHHHHCCCCCC
PSIVGLQHAILLAEKTREQKNAQYEEFKDIMVSVFQNEDITFEVNGNLEYRLPHVLNISF
CHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECCCEEEECCEEEEEEE
TGMNIEPFLVNLDLAGIAVSSGSACTAGSIDPSHVLVAMFGKDSDQIRSSVRFSFGLGNT
ECCCCCEEEEEEEEEEEEEECCCCCCCCCCCCCEEEEEEECCCHHHHHHHEEEEECCCCC
KEQIEKAAYETVKIVKRLTQN
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]