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The map label for this gene is alaS [H]

Identifier: 49187280

GI number: 49187280

Start: 4193017

End: 4195659

Strand: Reverse

Name: alaS [H]

Synonym: BAS4284

Alternate gene names: 49187280

Gene position: 4195659-4193017 (Counterclockwise)

Preceding gene: 49187282

Following gene: 49187279

Centisome position: 80.24

GC content: 39.54

Gene sequence:

>2643_bases
ATGAAACAACTAACAGGCGCACAAATTCGTCAAATGTTTTTAGACTTTTTCCAAGAAAAAGGACACGCAGTAGAACCTAG
TGCATCACTAGTTCCACATGAAGATCCATCTCTTCTGTGGATTAACAGTGGTGTAGCAACATTAAAAAAATATTTTGATG
GACGTGTAATCCCGCAAAATCCACGTATTACAAATGCTCAAAAATCAATTCGTACAAACGATATTGAAAACGTAGGGAAA
ACAGCTCGTCACCATACATTCTTTGAAATGTTAGGAAACTTCTCAATCGGTGATTACTTCAAAGAAGAAGCAATTACTTG
GGCTTGGGAATTCTTAACGAGCGACAAATGGATCGGATTCGATAAAGAATTACTATCCGTTACAATCCATCCAGAAGATG
AAGAAGCATTCACAATTTGGAATGAGAAAATGGGCGTTCCAAAAGAGCGCATCATCCGCTTAGAAGAAAACTTCTGGGAT
ATCGGTGAAGGACCAAGTGGACCGAATACAGAGATTTTCTATGACCGCGGAGAAGCTTACGGTAATGACTTTAGTGACCC
AGAATTATATCCAGGCGGAGAAAACGAGCGTTACTTAGAAGTATGGAACCTTGTATTCTCTCAATTTAACCATAATCCAG
ACGGTTCATATACACCACTTCCTAAGAAAAACATCGATACAGGGATGGGTCTAGAGCGTATGACATCTATCGTTCAAGAT
GTACCTACGAACTTTGATACAGACTTATTCATGCCAATGATTGGTGCAACAGAAACAATCTCTGGTGAGAAATATCGTAA
TGGTGACTTAGAAAAAGATATGGCGTTTAAAGTAATTGCTGACCATATCCGTACAGTAACATTCGCTGTTGGTGACGGTG
CTCTTCCATCTAACGAAGGCCGTGGTTATGTATTACGTCGTTTATTACGCCGTGCGGTTCGTTATTCGAAGAAATTAAAC
ATCAACCGTCCATTCATGTTTGAATTAGTGCCGGTTGTTGGTGAAGTAATGAAAGACTTCTATCCAGAAGTACTTGAAAA
GAAAGATTTCATCGCAAAAGTTGTGAAAAATGAAGAAGAGCGTTTCCATGAAACACTTCATGATGGAGAAGCGATTTTAT
CTGAAGTTATCGCAAAAGCTAAAGAAGAAAAAACAACTGTTATTTCTGGAGTAGATGCGTTCCGTCTATATGACACATAC
GGTTTCCCAATTGAATTAACAGAAGAATATGCAGAAGAAGCTGGTATGACAGTTGATCATGAAGGCTTTGAAAATGAAAT
GGAAAAACAACGTGAGCGCGCACGTGCTGCTCGTCAAGACGTTGATTCTATGCAAGTTCAAGGCGGTGTCCTTGGAGAAG
TGAAAGTAGCGAGCGAGTTCGTTGGTTACGGTACAGTTGCAACAGAAAGTAACGTTGTTGCACTTGTGAAAAACGGCGAA
TACACAGACAGCTTACAAGTTGGAGAAGAAGGACAATTAATGCTTGATGTAACACCATTCTATGCTGAGAGCGGTGGACA
AATTGCAGACCGTGGCTGTCTTCTTGCTGATGGCGTGAAAGTTCTTGTAAAAGACGTACAAAAAGCACCAAACGGCCAAA
ACTTGCACCAAGTTGTTGTGGAAGAAGGTACGTTAACGAAAGATGCAGCTGTAAAAGCTATTATCGATACGAAAAACCGT
AGTAGTGTTGTGAAAAACCATACAGCAACTCACTTACTACACCAAGCATTAAAAGATGTACTTGGAACGCACGTTAACCA
AGCGGGTTCTCTTGTAACTTCTGAACGTTTACGCTTTGACTTCTCTCACTTCGGTCAAGTACAAGCTGACGAATTAGAAA
AAATTGAGCGTATTGTAAACGAGAAAATTTGGGAAAGTATTGATGTTGAGATTTCTCAAAAAGCAATCGAAGAAGCGAAA
GAAATGGGCGCAATGGCATTATTCGGTGAAAAATATGGTGATGTTGTACGCGTTGTACAAGTTGGCGATTATAGCTTAGA
GCTTTGCGGTGGTTGTCACGTTGATAACACAGCTTCTATCGGTATTTTCAAAATCGTTGCTGAGTCTGGTATCGGTGCAG
GAATTCGTCGTATTGAAGCAGTAACTGGTAAGTCTGCTTATGAATTAATGAACGATCAAGTAGGTTTATTAAAAGAAGCA
GCTGGAAAAATGAAAACAAATCCAAAAGATATTTTAACAAGAGTAGATGGTTTGTTTGCTGAAGTAAAACAACTTCAAAA
GGAAAACGAATCTCTTGCAGCAAAATTAAGCAATATTGAAGCTGGAAACTTAACTGATTCAGTAATGACAGTTGATGGCG
TAAACGTATTAGCGGCAAAAGTAAATGTTGCAGATATGAACAACTTACGTACGATGATGGATGACCTAAAAAATAAATTA
GAATCTGCAGTTGTCGTATTAGCATCTGTAAATGATGATAAAGTAAACATCTTAGCTGGCGTAACGAAAGATTTAATTAG
CCAAGGATACCATGCTGGTAAACTTGTGAAAGAAGTAGCTTCTCGTTGTGGCGGTGGCGGTGGTGGCCGTCCTGATATGG
CACAAGCGGGCGGTAAAAACCCAGCGCAAGTTGAGGAAGCTTTAGCATTTGTACAAGAGTACGTTAAATCTGTTTCAAAA
TAA

Upstream 100 bases:

>100_bases
ATTCGTATAGCAATCAATTCATAATTAGGGTGGTATCGCGAGTTAACTCTCGTCCCTTTTATAGGGGCGGGAGTTTTTTA
TATTTAAGGAGGAAAATACA

Downstream 100 bases:

>100_bases
AGAGATAGTAGTGTACAATGGTAGGGAGAGGAGAAGTTCTTCTCCCTATACTTACTATATAAGTGAGGTGCTTGAAATGG
ACGGTTTTGATAAAACAATG

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 880; Mature: 880

Protein sequence:

>880_residues
MKQLTGAQIRQMFLDFFQEKGHAVEPSASLVPHEDPSLLWINSGVATLKKYFDGRVIPQNPRITNAQKSIRTNDIENVGK
TARHHTFFEMLGNFSIGDYFKEEAITWAWEFLTSDKWIGFDKELLSVTIHPEDEEAFTIWNEKMGVPKERIIRLEENFWD
IGEGPSGPNTEIFYDRGEAYGNDFSDPELYPGGENERYLEVWNLVFSQFNHNPDGSYTPLPKKNIDTGMGLERMTSIVQD
VPTNFDTDLFMPMIGATETISGEKYRNGDLEKDMAFKVIADHIRTVTFAVGDGALPSNEGRGYVLRRLLRRAVRYSKKLN
INRPFMFELVPVVGEVMKDFYPEVLEKKDFIAKVVKNEEERFHETLHDGEAILSEVIAKAKEEKTTVISGVDAFRLYDTY
GFPIELTEEYAEEAGMTVDHEGFENEMEKQRERARAARQDVDSMQVQGGVLGEVKVASEFVGYGTVATESNVVALVKNGE
YTDSLQVGEEGQLMLDVTPFYAESGGQIADRGCLLADGVKVLVKDVQKAPNGQNLHQVVVEEGTLTKDAAVKAIIDTKNR
SSVVKNHTATHLLHQALKDVLGTHVNQAGSLVTSERLRFDFSHFGQVQADELEKIERIVNEKIWESIDVEISQKAIEEAK
EMGAMALFGEKYGDVVRVVQVGDYSLELCGGCHVDNTASIGIFKIVAESGIGAGIRRIEAVTGKSAYELMNDQVGLLKEA
AGKMKTNPKDILTRVDGLFAEVKQLQKENESLAAKLSNIEAGNLTDSVMTVDGVNVLAAKVNVADMNNLRTMMDDLKNKL
ESAVVVLASVNDDKVNILAGVTKDLISQGYHAGKLVKEVASRCGGGGGGRPDMAQAGGKNPAQVEEALAFVQEYVKSVSK

Sequences:

>Translated_880_residues
MKQLTGAQIRQMFLDFFQEKGHAVEPSASLVPHEDPSLLWINSGVATLKKYFDGRVIPQNPRITNAQKSIRTNDIENVGK
TARHHTFFEMLGNFSIGDYFKEEAITWAWEFLTSDKWIGFDKELLSVTIHPEDEEAFTIWNEKMGVPKERIIRLEENFWD
IGEGPSGPNTEIFYDRGEAYGNDFSDPELYPGGENERYLEVWNLVFSQFNHNPDGSYTPLPKKNIDTGMGLERMTSIVQD
VPTNFDTDLFMPMIGATETISGEKYRNGDLEKDMAFKVIADHIRTVTFAVGDGALPSNEGRGYVLRRLLRRAVRYSKKLN
INRPFMFELVPVVGEVMKDFYPEVLEKKDFIAKVVKNEEERFHETLHDGEAILSEVIAKAKEEKTTVISGVDAFRLYDTY
GFPIELTEEYAEEAGMTVDHEGFENEMEKQRERARAARQDVDSMQVQGGVLGEVKVASEFVGYGTVATESNVVALVKNGE
YTDSLQVGEEGQLMLDVTPFYAESGGQIADRGCLLADGVKVLVKDVQKAPNGQNLHQVVVEEGTLTKDAAVKAIIDTKNR
SSVVKNHTATHLLHQALKDVLGTHVNQAGSLVTSERLRFDFSHFGQVQADELEKIERIVNEKIWESIDVEISQKAIEEAK
EMGAMALFGEKYGDVVRVVQVGDYSLELCGGCHVDNTASIGIFKIVAESGIGAGIRRIEAVTGKSAYELMNDQVGLLKEA
AGKMKTNPKDILTRVDGLFAEVKQLQKENESLAAKLSNIEAGNLTDSVMTVDGVNVLAAKVNVADMNNLRTMMDDLKNKL
ESAVVVLASVNDDKVNILAGVTKDLISQGYHAGKLVKEVASRCGGGGGGRPDMAQAGGKNPAQVEEALAFVQEYVKSVSK
>Mature_880_residues
MKQLTGAQIRQMFLDFFQEKGHAVEPSASLVPHEDPSLLWINSGVATLKKYFDGRVIPQNPRITNAQKSIRTNDIENVGK
TARHHTFFEMLGNFSIGDYFKEEAITWAWEFLTSDKWIGFDKELLSVTIHPEDEEAFTIWNEKMGVPKERIIRLEENFWD
IGEGPSGPNTEIFYDRGEAYGNDFSDPELYPGGENERYLEVWNLVFSQFNHNPDGSYTPLPKKNIDTGMGLERMTSIVQD
VPTNFDTDLFMPMIGATETISGEKYRNGDLEKDMAFKVIADHIRTVTFAVGDGALPSNEGRGYVLRRLLRRAVRYSKKLN
INRPFMFELVPVVGEVMKDFYPEVLEKKDFIAKVVKNEEERFHETLHDGEAILSEVIAKAKEEKTTVISGVDAFRLYDTY
GFPIELTEEYAEEAGMTVDHEGFENEMEKQRERARAARQDVDSMQVQGGVLGEVKVASEFVGYGTVATESNVVALVKNGE
YTDSLQVGEEGQLMLDVTPFYAESGGQIADRGCLLADGVKVLVKDVQKAPNGQNLHQVVVEEGTLTKDAAVKAIIDTKNR
SSVVKNHTATHLLHQALKDVLGTHVNQAGSLVTSERLRFDFSHFGQVQADELEKIERIVNEKIWESIDVEISQKAIEEAK
EMGAMALFGEKYGDVVRVVQVGDYSLELCGGCHVDNTASIGIFKIVAESGIGAGIRRIEAVTGKSAYELMNDQVGLLKEA
AGKMKTNPKDILTRVDGLFAEVKQLQKENESLAAKLSNIEAGNLTDSVMTVDGVNVLAAKVNVADMNNLRTMMDDLKNKL
ESAVVVLASVNDDKVNILAGVTKDLISQGYHAGKLVKEVASRCGGGGGGRPDMAQAGGKNPAQVEEALAFVQEYVKSVSK

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=976, Percent_Identity=35.5532786885246, Blast_Score=487, Evalue=1e-137,
Organism=Homo sapiens, GI38569417, Length=964, Percent_Identity=31.6390041493776, Blast_Score=378, Evalue=1e-104,
Organism=Escherichia coli, GI1789048, Length=889, Percent_Identity=44.7694038245219, Blast_Score=738, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=984, Percent_Identity=35.6707317073171, Blast_Score=513, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17536681, Length=748, Percent_Identity=34.6256684491979, Blast_Score=371, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6324911, Length=963, Percent_Identity=36.7601246105919, Blast_Score=524, Evalue=1e-149,
Organism=Drosophila melanogaster, GI24582809, Length=975, Percent_Identity=34.7692307692308, Blast_Score=494, Evalue=1e-139,
Organism=Drosophila melanogaster, GI45552267, Length=975, Percent_Identity=34.7692307692308, Blast_Score=494, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24658214, Length=807, Percent_Identity=29.4919454770756, Blast_Score=293, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 97442; Mature: 97442

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA ; PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLTGAQIRQMFLDFFQEKGHAVEPSASLVPHEDPSLLWINSGVATLKKYFDGRVIPQN
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCC
PRITNAQKSIRTNDIENVGKTARHHTFFEMLGNFSIGDYFKEEAITWAWEFLTSDKWIGF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
DKELLSVTIHPEDEEAFTIWNEKMGVPKERIIRLEENFWDIGEGPSGPNTEIFYDRGEAY
CHHHEEEEECCCCCCEEEEEHHHCCCCHHHHEEHHHCCCCCCCCCCCCCCCEEEECCCCC
GNDFSDPELYPGGENERYLEVWNLVFSQFNHNPDGSYTPLPKKNIDTGMGLERMTSIVQD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VPTNFDTDLFMPMIGATETISGEKYRNGDLEKDMAFKVIADHIRTVTFAVGDGALPSNEG
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCC
RGYVLRRLLRRAVRYSKKLNINRPFMFELVPVVGEVMKDFYPEVLEKKDFIAKVVKNEEE
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
RFHETLHDGEAILSEVIAKAKEEKTTVISGVDAFRLYDTYGFPIELTEEYAEEAGMTVDH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCEEHHHHHHHHCCCEECC
EGFENEMEKQRERARAARQDVDSMQVQGGVLGEVKVASEFVGYGTVATESNVVALVKNGE
CCHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHCCCCEECCCCEEEEEECCC
YTDSLQVGEEGQLMLDVTPFYAESGGQIADRGCLLADGVKVLVKDVQKAPNGQNLHQVVV
CCCCCCCCCCCCEEEEECHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
EEGTLTKDAAVKAIIDTKNRSSVVKNHTATHLLHQALKDVLGTHVNQAGSLVTSERLRFD
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
FSHFGQVQADELEKIERIVNEKIWESIDVEISQKAIEEAKEMGAMALFGEKYGDVVRVVQ
HHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEE
VGDYSLELCGGCHVDNTASIGIFKIVAESGIGAGIRRIEAVTGKSAYELMNDQVGLLKEA
ECCCEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AGKMKTNPKDILTRVDGLFAEVKQLQKENESLAAKLSNIEAGNLTDSVMTVDGVNVLAAK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEE
VNVADMNNLRTMMDDLKNKLESAVVVLASVNDDKVNILAGVTKDLISQGYHAGKLVKEVA
EEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEHHHHHHHHHHHCCCHHHHHHHHHH
SRCGGGGGGRPDMAQAGGKNPAQVEEALAFVQEYVKSVSK
HHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKQLTGAQIRQMFLDFFQEKGHAVEPSASLVPHEDPSLLWINSGVATLKKYFDGRVIPQN
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCC
PRITNAQKSIRTNDIENVGKTARHHTFFEMLGNFSIGDYFKEEAITWAWEFLTSDKWIGF
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
DKELLSVTIHPEDEEAFTIWNEKMGVPKERIIRLEENFWDIGEGPSGPNTEIFYDRGEAY
CHHHEEEEECCCCCCEEEEEHHHCCCCHHHHEEHHHCCCCCCCCCCCCCCCEEEECCCCC
GNDFSDPELYPGGENERYLEVWNLVFSQFNHNPDGSYTPLPKKNIDTGMGLERMTSIVQD
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
VPTNFDTDLFMPMIGATETISGEKYRNGDLEKDMAFKVIADHIRTVTFAVGDGALPSNEG
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEEEEEECCCCCCCCCC
RGYVLRRLLRRAVRYSKKLNINRPFMFELVPVVGEVMKDFYPEVLEKKDFIAKVVKNEEE
CCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHH
RFHETLHDGEAILSEVIAKAKEEKTTVISGVDAFRLYDTYGFPIELTEEYAEEAGMTVDH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCCCEEHHHHHHHHCCCEECC
EGFENEMEKQRERARAARQDVDSMQVQGGVLGEVKVASEFVGYGTVATESNVVALVKNGE
CCHHHHHHHHHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHCCCCEECCCCEEEEEECCC
YTDSLQVGEEGQLMLDVTPFYAESGGQIADRGCLLADGVKVLVKDVQKAPNGQNLHQVVV
CCCCCCCCCCCCEEEEECHHHHCCCCCCHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHHH
EEGTLTKDAAVKAIIDTKNRSSVVKNHTATHLLHQALKDVLGTHVNQAGSLVTSERLRFD
CCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHC
FSHFGQVQADELEKIERIVNEKIWESIDVEISQKAIEEAKEMGAMALFGEKYGDVVRVVQ
HHHCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHCCEEEEEE
VGDYSLELCGGCHVDNTASIGIFKIVAESGIGAGIRRIEAVTGKSAYELMNDQVGLLKEA
ECCCEEEECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHH
AGKMKTNPKDILTRVDGLFAEVKQLQKENESLAAKLSNIEAGNLTDSVMTVDGVNVLAAK
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCEEEEEE
VNVADMNNLRTMMDDLKNKLESAVVVLASVNDDKVNILAGVTKDLISQGYHAGKLVKEVA
EEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCEEHHHHHHHHHHHCCCHHHHHHHHHH
SRCGGGGGGRPDMAQAGGKNPAQVEEALAFVQEYVKSVSK
HHCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA