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The map label for this gene is yrrO [H]

Identifier: 49187273

GI number: 49187273

Start: 4187598

End: 4188878

Strand: Reverse

Name: yrrO [H]

Synonym: BAS4277

Alternate gene names: 49187273

Gene position: 4188878-4187598 (Counterclockwise)

Preceding gene: 49187274

Following gene: 49187272

Centisome position: 80.11

GC content: 38.25

Gene sequence:

>1281_bases
ATGACTGTACAAGAAATTTCACGAGTAATCGATGGCAAACGCGTTATTGTGAAGAAACCTGAACTGTTAATCCCTGCGGG
TAACTTAGAAAAATTAAAAGTAGCTATCCATTACGGGGCAGATGCTGTATATTTAGGTGGACAAGAATTTGGTCTTCGTT
CGAATGCAGGTAACTTTACACTGGAAGAAATGGCAGAAGGTGTGGAATTCGCAAAGAAATATGGAGCGAAAATATATGTA
ACAACAAATATCTTTGCGCATAATGAAAATATGGACGGGCTAGAGGAATATTTAAAAGGGATTGAAAAAGCTGGCGTAAC
GGGAATTATCGTTGCTGATCCGCTTATTATTGAGACTTGTAAACGTGTAGCACCTTCTGTTGAGGTGCATTTAAGTACAC
AACAATCACTATCCAACTGGAAAGCAGCACAGTATTGGAAAGAAGAAGGTTTACATCGTCTTGTATTAGCTCGTGAAGCA
AGCTATGAAGAAATGAAAGAAATTAAAGAAAAAGTGGATATTGAGATTGAAGCATTCGTCCATGGTGCAATGTGTATCGC
ATATTCAGGAAGATGTACGTTAAGTAACCATATGACAGCGCGTGATTCTAACCGTGGTGGTTGTTGTCAATCTTGCCGCT
GGGACTATGATTTAGTTCAAACAGTATCACAACATAAAGATGCAAAAGAGCTTCCTCTATTCCAAGAAGAAGATGCTCAC
TTCGCGATGAGTCCAAAAGACTTAAATTTAATTTTATCAATTCCGAAAATGATTGAAATTGGAATTGATAGCTTAAAAGT
TGAAGGACGTATGAAATCAATCCATTACGTAGCGACTGTAGCGACAGTATACCGTAAAGTAATTGATGCATATTGTGCGG
ATCCTGATAACTTTGAGTTTAAGCAAGAATGGTTAGATGAGCTTGATAAATGTGCAAATCGTGATACAGCTCCTGCATTC
TTTGAAGGGGTTCCAGGACATCAAGAGCAAATGTTTGGAAATCATAGTAAGAAAACAACGTATGATTTCGCTGGTTTAGT
GTTAGATTATAATGAAGAAACGGGCATCGTAACGATTGAGCAACGTAATCATTTCAAACCAGGACATGAAGTGGAGTTCT
TTGGACCAGAAATAGAAAACTTTACGCAGACGGTGGAGAAAATTTGGGATGAGGATGGAAACGAATTAGATGCAGCGAGA
CATCCGTTGCAGATCGTGAAATTCAAAGTGGATCAACCAGTGTATGTGAATAATATGATGCGCAAAAGCATACTTCAATA
A

Upstream 100 bases:

>100_bases
TTTATATAAAGAGATAGAAGAAATGCAACCGGTAAATCGTCCGTTAGATACAGGATTCTTCTTTAAAGAGACGGTTTACT
AAACGAGGAGGGTGTAGGAA

Downstream 100 bases:

>100_bases
GAAAGAGTGATAGTCGAATGGGGACGAATAAGCCTGTTGTAATTGGAATTGCTGGTGGTTCAGGGTCAGGTAAGACAAGT
GTAACGAAAGCGATTTTTGA

Product: U32 family peptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MTVQEISRVIDGKRVIVKKPELLIPAGNLEKLKVAIHYGADAVYLGGQEFGLRSNAGNFTLEEMAEGVEFAKKYGAKIYV
TTNIFAHNENMDGLEEYLKGIEKAGVTGIIVADPLIIETCKRVAPSVEVHLSTQQSLSNWKAAQYWKEEGLHRLVLAREA
SYEEMKEIKEKVDIEIEAFVHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYDLVQTVSQHKDAKELPLFQEEDAH
FAMSPKDLNLILSIPKMIEIGIDSLKVEGRMKSIHYVATVATVYRKVIDAYCADPDNFEFKQEWLDELDKCANRDTAPAF
FEGVPGHQEQMFGNHSKKTTYDFAGLVLDYNEETGIVTIEQRNHFKPGHEVEFFGPEIENFTQTVEKIWDEDGNELDAAR
HPLQIVKFKVDQPVYVNNMMRKSILQ

Sequences:

>Translated_426_residues
MTVQEISRVIDGKRVIVKKPELLIPAGNLEKLKVAIHYGADAVYLGGQEFGLRSNAGNFTLEEMAEGVEFAKKYGAKIYV
TTNIFAHNENMDGLEEYLKGIEKAGVTGIIVADPLIIETCKRVAPSVEVHLSTQQSLSNWKAAQYWKEEGLHRLVLAREA
SYEEMKEIKEKVDIEIEAFVHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYDLVQTVSQHKDAKELPLFQEEDAH
FAMSPKDLNLILSIPKMIEIGIDSLKVEGRMKSIHYVATVATVYRKVIDAYCADPDNFEFKQEWLDELDKCANRDTAPAF
FEGVPGHQEQMFGNHSKKTTYDFAGLVLDYNEETGIVTIEQRNHFKPGHEVEFFGPEIENFTQTVEKIWDEDGNELDAAR
HPLQIVKFKVDQPVYVNNMMRKSILQ
>Mature_425_residues
TVQEISRVIDGKRVIVKKPELLIPAGNLEKLKVAIHYGADAVYLGGQEFGLRSNAGNFTLEEMAEGVEFAKKYGAKIYVT
TNIFAHNENMDGLEEYLKGIEKAGVTGIIVADPLIIETCKRVAPSVEVHLSTQQSLSNWKAAQYWKEEGLHRLVLAREAS
YEEMKEIKEKVDIEIEAFVHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYDLVQTVSQHKDAKELPLFQEEDAHF
AMSPKDLNLILSIPKMIEIGIDSLKVEGRMKSIHYVATVATVYRKVIDAYCADPDNFEFKQEWLDELDKCANRDTAPAFF
EGVPGHQEQMFGNHSKKTTYDFAGLVLDYNEETGIVTIEQRNHFKPGHEVEFFGPEIENFTQTVEKIWDEDGNELDAARH
PLQIVKFKVDQPVYVNNMMRKSILQ

Specific function: Unknown

COG id: COG0826

COG function: function code O; Collagenase and related proteases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U32 family [H]

Homologues:

Organism=Escherichia coli, GI1788397, Length=446, Percent_Identity=32.0627802690583, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI87081910, Length=270, Percent_Identity=38.1481481481481, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789548, Length=314, Percent_Identity=32.8025477707006, Blast_Score=156, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001539 [H]

Pfam domain/function: PF01136 Peptidase_U32 [H]

EC number: 3.4.-.- [C]

Molecular weight: Translated: 48266; Mature: 48135

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS01276 PEPTIDASE_U32

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVQEISRVIDGKRVIVKKPELLIPAGNLEKLKVAIHYGADAVYLGGQEFGLRSNAGNFT
CCHHHHHHHHCCCEEEEECCCEEEECCCCEEEEEEEEECCCEEEECCCCCCCCCCCCCCC
LEEMAEGVEFAKKYGAKIYVTTNIFAHNENMDGLEEYLKGIEKAGVTGIIVADPLIIETC
HHHHHHHHHHHHHHCCEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHH
KRVAPSVEVHLSTQQSLSNWKAAQYWKEEGLHRLVLAREASYEEMKEIKEKVDIEIEAFV
HHHCCCEEEEECCHHHHHCCHHHHHHHHCCHHEEEHHHCCCHHHHHHHHHHHCEEEEHEE
HGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYDLVQTVSQHKDAKELPLFQEEDAH
EEEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCC
FAMSPKDLNLILSIPKMIEIGIDSLKVEGRMKSIHYVATVATVYRKVIDAYCADPDNFEF
EEECCCCCEEEEECHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
KQEWLDELDKCANRDTAPAFFEGVPGHQEQMFGNHSKKTTYDFAGLVLDYNEETGIVTIE
HHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHCCCCCCCCEEEEEEEEEEECCCCCEEEEE
QRNHFKPGHEVEFFGPEIENFTQTVEKIWDEDGNELDAARHPLQIVKFKVDQPVYVNNMM
CCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHCCEEEEEEECCCCHHHHHHH
RKSILQ
HHHHCC
>Mature Secondary Structure 
TVQEISRVIDGKRVIVKKPELLIPAGNLEKLKVAIHYGADAVYLGGQEFGLRSNAGNFT
CHHHHHHHHCCCEEEEECCCEEEECCCCEEEEEEEEECCCEEEECCCCCCCCCCCCCCC
LEEMAEGVEFAKKYGAKIYVTTNIFAHNENMDGLEEYLKGIEKAGVTGIIVADPLIIETC
HHHHHHHHHHHHHHCCEEEEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHH
KRVAPSVEVHLSTQQSLSNWKAAQYWKEEGLHRLVLAREASYEEMKEIKEKVDIEIEAFV
HHHCCCEEEEECCHHHHHCCHHHHHHHHCCHHEEEHHHCCCHHHHHHHHHHHCEEEEHEE
HGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYDLVQTVSQHKDAKELPLFQEEDAH
EEEEEEEECCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHHCCCCCCCCCC
FAMSPKDLNLILSIPKMIEIGIDSLKVEGRMKSIHYVATVATVYRKVIDAYCADPDNFEF
EEECCCCCEEEEECHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
KQEWLDELDKCANRDTAPAFFEGVPGHQEQMFGNHSKKTTYDFAGLVLDYNEETGIVTIE
HHHHHHHHHHHCCCCCCCHHHCCCCCHHHHHCCCCCCCCEEEEEEEEEEECCCCCEEEEE
QRNHFKPGHEVEFFGPEIENFTQTVEKIWDEDGNELDAARHPLQIVKFKVDQPVYVNNMM
CCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHCCEEEEEEECCCCHHHHHHH
RKSILQ
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]