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The map label for this gene is bfmBC [H]

Identifier: 49187066

GI number: 49187066

Start: 3999195

End: 4000616

Strand: Reverse

Name: bfmBC [H]

Synonym: BAS4068

Alternate gene names: 49187066

Gene position: 4000616-3999195 (Counterclockwise)

Preceding gene: 49187067

Following gene: 49187065

Centisome position: 76.51

GC content: 38.47

Gene sequence:

>1422_bases
ATGGCAAAAGAATATGATTTAGTCATCGTTGGCGGCGGTACTGGCGGATATGTTGCTGCAATTCGTGCATCACAACTAGG
TTTAAAAACTGCACTTGTTGAAAAAGAAAATCTTGGTGGTACTTGTTTACACAAAGGATGTATTCCTAGTAAAGCCCTTT
TACGTAGTGCGGAAGTATACGCAACTGCTAAAAAAAGCGAAGAGTTCGGGGTTATTGCAAGTAATGTAGAACTAAACTTT
GCGAAAGTACAAGAGCGTAAAGAGAAGATTGTAACGCAACTTCATAAAGGTGTTCAGCACTTAATGAAACAAGGTAAAAT
TGATGTGTTTGAAGGTATTGGCCGTATTCTTGGCCCATCTATTTTCTCTCCGATGCCAGGGACAATTTCAGTTGAACTTG
CAAGTGGAGAAGAGAATGAAATGTTAATTCCAAAAAATGTACTTATTGCAACAGGTTCTCGTCCGAATTCATTACCAGGT
TTAGAATTAGACGGAGAGTATGTAATGTCTTCCGATCATGCCCTAAAAATGGAAACGCTTCCCAGCTCGATTATTATCGT
TGGTGGCGGTGTAATTGGTATCGAGTGGGCATCTATGCTTGCTGACTTCGGTGTAGAAGTTACAGTATTAGAGTATGCGA
AAACGATACTACCATTAGAAGATCAAGACGTTTCAAAAGAAATGCAACGTCTATTCAAGAAAAAAGGTATTAAAGTGGTA
ACTGGTGCAAAAGTATTACCAGAAACATTGGTAAAAGATAATGGAGTAACAATTCAAGCTGAACATAACGGTGAGAATAA
AGAATTTAAAGCAGAAAAAATGCTTGTATCTGTAGGACGACAAGCCAATACGCAAAATATTGGTTTAGAGAATACGGATA
TCGTTGTGGAAAAAGGATACATTCAAACAAATGAGTTTTATCAAACGAAAGAATCTCATATTTACGCAATCGGTGATGTA
ATCGGTGGCTTACAGCTTGCTCACGTTGCTTCTCATGAAGGAATTGTTGCAGTAGAACATATTGCTGGTAAAGAAGTTAC
ACCAATTGATTATTCTATGGTATCAAAATGCGTATATAGCAGTCCGGAAGTTGCTTCTGTCGGTTTAACAGAACAAGAAG
CGAAAGAAAAAGGCTATAAGTTAAAAGTAGGTAAGTTCTCATTCCGTGCAATCGGGAAAGCACTTGTATACGGCGAATCA
GATGGTTTCGTAAAACTTGTAGTTGATGAAGAAACAAATGACATTCTCGGTGTTCATATGATTGGACCACATGTAACAGA
TATGATTTCTGAAGCAGGTCTTGCAAGAGTACTTGATGCAACACCTTGGGAAGTAGCACATACAATTCATCCGCATCCAT
CATTATCTGAGGCGATTGGTGAAGCAGCATTAGCTGTAGATGGAAAAGCGTTACACGCATAA

Upstream 100 bases:

>100_bases
CAAGCATTAGCAGAAGGGGCACTTCGTGTACTACGTGAAGAAGAAGCTCCAAAAGAATATATTGTACGTGAAAAAGAAAC
GGTAGCTAGGGGTTGAGATA

Downstream 100 bases:

>100_bases
AAATGTGGATTTAGGAGGTTATGAAAAATGGCAGAAGTAAAAGAAAAGCGCCATGAAGAGCTTGGCTTAAGTGATGAGCA
AGTGTTAGAAATGTTCCGTA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MAKEYDLVIVGGGTGGYVAAIRASQLGLKTALVEKENLGGTCLHKGCIPSKALLRSAEVYATAKKSEEFGVIASNVELNF
AKVQERKEKIVTQLHKGVQHLMKQGKIDVFEGIGRILGPSIFSPMPGTISVELASGEENEMLIPKNVLIATGSRPNSLPG
LELDGEYVMSSDHALKMETLPSSIIIVGGGVIGIEWASMLADFGVEVTVLEYAKTILPLEDQDVSKEMQRLFKKKGIKVV
TGAKVLPETLVKDNGVTIQAEHNGENKEFKAEKMLVSVGRQANTQNIGLENTDIVVEKGYIQTNEFYQTKESHIYAIGDV
IGGLQLAHVASHEGIVAVEHIAGKEVTPIDYSMVSKCVYSSPEVASVGLTEQEAKEKGYKLKVGKFSFRAIGKALVYGES
DGFVKLVVDEETNDILGVHMIGPHVTDMISEAGLARVLDATPWEVAHTIHPHPSLSEAIGEAALAVDGKALHA

Sequences:

>Translated_473_residues
MAKEYDLVIVGGGTGGYVAAIRASQLGLKTALVEKENLGGTCLHKGCIPSKALLRSAEVYATAKKSEEFGVIASNVELNF
AKVQERKEKIVTQLHKGVQHLMKQGKIDVFEGIGRILGPSIFSPMPGTISVELASGEENEMLIPKNVLIATGSRPNSLPG
LELDGEYVMSSDHALKMETLPSSIIIVGGGVIGIEWASMLADFGVEVTVLEYAKTILPLEDQDVSKEMQRLFKKKGIKVV
TGAKVLPETLVKDNGVTIQAEHNGENKEFKAEKMLVSVGRQANTQNIGLENTDIVVEKGYIQTNEFYQTKESHIYAIGDV
IGGLQLAHVASHEGIVAVEHIAGKEVTPIDYSMVSKCVYSSPEVASVGLTEQEAKEKGYKLKVGKFSFRAIGKALVYGES
DGFVKLVVDEETNDILGVHMIGPHVTDMISEAGLARVLDATPWEVAHTIHPHPSLSEAIGEAALAVDGKALHA
>Mature_472_residues
AKEYDLVIVGGGTGGYVAAIRASQLGLKTALVEKENLGGTCLHKGCIPSKALLRSAEVYATAKKSEEFGVIASNVELNFA
KVQERKEKIVTQLHKGVQHLMKQGKIDVFEGIGRILGPSIFSPMPGTISVELASGEENEMLIPKNVLIATGSRPNSLPGL
ELDGEYVMSSDHALKMETLPSSIIIVGGGVIGIEWASMLADFGVEVTVLEYAKTILPLEDQDVSKEMQRLFKKKGIKVVT
GAKVLPETLVKDNGVTIQAEHNGENKEFKAEKMLVSVGRQANTQNIGLENTDIVVEKGYIQTNEFYQTKESHIYAIGDVI
GGLQLAHVASHEGIVAVEHIAGKEVTPIDYSMVSKCVYSSPEVASVGLTEQEAKEKGYKLKVGKFSFRAIGKALVYGESD
GFVKLVVDEETNDILGVHMIGPHVTDMISEAGLARVLDATPWEVAHTIHPHPSLSEAIGEAALAVDGKALHA

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=477, Percent_Identity=40.041928721174, Blast_Score=320, Evalue=2e-87,
Organism=Homo sapiens, GI50301238, Length=470, Percent_Identity=28.2978723404255, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI33519430, Length=445, Percent_Identity=25.1685393258427, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI33519428, Length=445, Percent_Identity=25.1685393258427, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI33519426, Length=445, Percent_Identity=25.1685393258427, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI148277065, Length=445, Percent_Identity=25.1685393258427, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI148277071, Length=445, Percent_Identity=25.1685393258427, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI22035672, Length=485, Percent_Identity=26.1855670103093, Blast_Score=126, Evalue=3e-29,
Organism=Homo sapiens, GI291045266, Length=437, Percent_Identity=24.7139588100687, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI291045268, Length=434, Percent_Identity=22.5806451612903, Blast_Score=96, Evalue=7e-20,
Organism=Escherichia coli, GI1786307, Length=466, Percent_Identity=34.9785407725322, Blast_Score=262, Evalue=3e-71,
Organism=Escherichia coli, GI87082354, Length=466, Percent_Identity=27.6824034334764, Blast_Score=189, Evalue=4e-49,
Organism=Escherichia coli, GI87081717, Length=468, Percent_Identity=28.8461538461538, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1789915, Length=467, Percent_Identity=28.2655246252677, Blast_Score=166, Evalue=4e-42,
Organism=Caenorhabditis elegans, GI32565766, Length=478, Percent_Identity=41.6317991631799, Blast_Score=332, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI17557007, Length=489, Percent_Identity=25.7668711656442, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71983429, Length=469, Percent_Identity=26.0127931769723, Blast_Score=113, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=469, Percent_Identity=26.0127931769723, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=491, Percent_Identity=25.4582484725051, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=483, Percent_Identity=38.9233954451346, Blast_Score=293, Evalue=4e-80,
Organism=Saccharomyces cerevisiae, GI6325240, Length=482, Percent_Identity=28.6307053941909, Blast_Score=171, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6325166, Length=475, Percent_Identity=27.1578947368421, Blast_Score=160, Evalue=5e-40,
Organism=Drosophila melanogaster, GI21358499, Length=468, Percent_Identity=41.8803418803419, Blast_Score=332, Evalue=3e-91,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=24.8459958932238, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24640549, Length=487, Percent_Identity=24.8459958932238, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24640551, Length=487, Percent_Identity=24.8459958932238, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI17737741, Length=474, Percent_Identity=22.3628691983122, Blast_Score=103, Evalue=2e-22,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50775; Mature: 50644

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEYDLVIVGGGTGGYVAAIRASQLGLKTALVEKENLGGTCLHKGCIPSKALLRSAEVY
CCCEEEEEEEECCCCCEEEEEEHHHCCHHHEEEECCCCCCCEEECCCCCHHHHHHHHHHE
ATAKKSEEFGVIASNVELNFAKVQERKEKIVTQLHKGVQHLMKQGKIDVFEGIGRILGPS
EEECCCCCCCEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHH
IFSPMPGTISVELASGEENEMLIPKNVLIATGSRPNSLPGLELDGEYVMSSDHALKMETL
HCCCCCCEEEEEEECCCCCCEECCCCEEEEECCCCCCCCCEEECCCEEECCCCEEEEECC
PSSIIIVGGGVIGIEWASMLADFGVEVTVLEYAKTILPLEDQDVSKEMQRLFKKKGIKVV
CCEEEEEECCEEEHHHHHHHHHCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCEEE
TGAKVLPETLVKDNGVTIQAEHNGENKEFKAEKMLVSVGRQANTQNIGLENTDIVVEKGY
ECCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCE
IQTNEFYQTKESHIYAIGDVIGGLQLAHVASHEGIVAVEHIAGKEVTPIDYSMVSKCVYS
EECCHHHHCCCCEEEEEHHHHCCHHEEEHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCC
SPEVASVGLTEQEAKEKGYKLKVGKFSFRAIGKALVYGESDGFVKLVVDEETNDILGVHM
CCCEEECCCCHHHHHHCCCEEEECCCHHHHHCEEEEEECCCCEEEEEEECCCCCEEEEEE
IGPHVTDMISEAGLARVLDATPWEVAHTIHPHPSLSEAIGEAALAVDGKALHA
ECCHHHHHHHHCCHHHHHCCCCHHHEEECCCCCCHHHHHCCCEEEECCHHCCC
>Mature Secondary Structure 
AKEYDLVIVGGGTGGYVAAIRASQLGLKTALVEKENLGGTCLHKGCIPSKALLRSAEVY
CCEEEEEEEECCCCCEEEEEEHHHCCHHHEEEECCCCCCCEEECCCCCHHHHHHHHHHE
ATAKKSEEFGVIASNVELNFAKVQERKEKIVTQLHKGVQHLMKQGKIDVFEGIGRILGPS
EEECCCCCCCEEEECCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCHH
IFSPMPGTISVELASGEENEMLIPKNVLIATGSRPNSLPGLELDGEYVMSSDHALKMETL
HCCCCCCEEEEEEECCCCCCEECCCCEEEEECCCCCCCCCEEECCCEEECCCCEEEEECC
PSSIIIVGGGVIGIEWASMLADFGVEVTVLEYAKTILPLEDQDVSKEMQRLFKKKGIKVV
CCEEEEEECCEEEHHHHHHHHHCCCEEEEEHHHHHHCCCCCCHHHHHHHHHHHHCCCEEE
TGAKVLPETLVKDNGVTIQAEHNGENKEFKAEKMLVSVGRQANTQNIGLENTDIVVEKGY
ECCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCE
IQTNEFYQTKESHIYAIGDVIGGLQLAHVASHEGIVAVEHIAGKEVTPIDYSMVSKCVYS
EECCHHHHCCCCEEEEEHHHHCCHHEEEHHCCCCEEEEEECCCCCCCCCCHHHHHHHHCC
SPEVASVGLTEQEAKEKGYKLKVGKFSFRAIGKALVYGESDGFVKLVVDEETNDILGVHM
CCCEEECCCCHHHHHHCCCEEEECCCHHHHHCEEEEEECCCCEEEEEEECCCCCEEEEEE
IGPHVTDMISEAGLARVLDATPWEVAHTIHPHPSLSEAIGEAALAVDGKALHA
ECCHHHHHHHHCCHHHHHCCCCHHHEEECCCCCCHHHHHCCCEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8504804 [H]