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The map label for this gene is cof [H]

Identifier: 49186960

GI number: 49186960

Start: 3907829

End: 3908635

Strand: Direct

Name: cof [H]

Synonym: BAS3962

Alternate gene names: 49186960

Gene position: 3907829-3908635 (Clockwise)

Preceding gene: 49186955

Following gene: 49186967

Centisome position: 74.74

GC content: 34.82

Gene sequence:

>807_bases
ATGATTAAAATGTTTGTAAGTGATATCGATGGTACAATGATGCAACACGGGGGTATTATTGATGAGCAAGATGTTGCGGC
ACTTCGCAGTCTTGCTGAGCAAAATGTTATTCTTTGCTTCGCCTCTGGACGACTTGATAATGAAATTGCAGACTTAATGA
AAGCTGTAAATACAAATTTCCATCGTATTAGTGTGAATGGTGTTTTCGTATATACAGATGAAAATAAGCAACTCTTATCT
GCAACTTTTGATTCCAGCATTCTTCCTGAGTTGTTAGACATGACAAATGAAGATCCTTATTTCCGTTATGTTAGTGATGA
ACATAATTACTATATTGAAGAGAAGACACCGTTCATTCATGAACTTGAAAAACAAGTAACGATGACTTCTGTTGAAGAAC
CAAATTTATTACAGAAAATAGATGATACAATTTTTCCAAATAAAATTTCTGTGGGTGGAACAAAGGAGAGTTTGCAACTC
CTTCAGAAAAAAATTGATGAAAAATTCCACGGAAAAGTGAGTACCTTCATTTCAGCAGAACAATGTTTAGATGTAATGCC
ACCGAATATTAGTAAAGGCTCCGCTATTTCAGTTTTATTAAAAGAGTTTCAATTACAACCAGAGGAAGTTGCTTGCATAG
GGGATTCTTATAATGATATTCCAATGTTTTCTTTAACTCCTCACAGTTTTGCTATGGCTCAGGCAGATGACGCAGTAAAA
GAACACGCTCACTATGTAGTAAATACAGTTAAAGATGCTGTTAACCACGTAATTGCTCATAATAAAAATACGACTCACTC
CTTGTAA

Upstream 100 bases:

>100_bases
TGTAAAACATCTACCATTTCATAAAAATTCATTTTTTAGACATGTTTATATATGATGCGTATAATGTAAGAGCAACTGTA
CATAGAAAGGAGATAACAAT

Downstream 100 bases:

>100_bases
GGATGAGTCGTATTTTTTCTTTACATGTTAAACAATTTGACATTTACTGTACTTCACAAACTGAAATATATTATTTGTTA
CGTGCGATGTTCGTAATAAA

Product: HAD superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MIKMFVSDIDGTMMQHGGIIDEQDVAALRSLAEQNVILCFASGRLDNEIADLMKAVNTNFHRISVNGVFVYTDENKQLLS
ATFDSSILPELLDMTNEDPYFRYVSDEHNYYIEEKTPFIHELEKQVTMTSVEEPNLLQKIDDTIFPNKISVGGTKESLQL
LQKKIDEKFHGKVSTFISAEQCLDVMPPNISKGSAISVLLKEFQLQPEEVACIGDSYNDIPMFSLTPHSFAMAQADDAVK
EHAHYVVNTVKDAVNHVIAHNKNTTHSL

Sequences:

>Translated_268_residues
MIKMFVSDIDGTMMQHGGIIDEQDVAALRSLAEQNVILCFASGRLDNEIADLMKAVNTNFHRISVNGVFVYTDENKQLLS
ATFDSSILPELLDMTNEDPYFRYVSDEHNYYIEEKTPFIHELEKQVTMTSVEEPNLLQKIDDTIFPNKISVGGTKESLQL
LQKKIDEKFHGKVSTFISAEQCLDVMPPNISKGSAISVLLKEFQLQPEEVACIGDSYNDIPMFSLTPHSFAMAQADDAVK
EHAHYVVNTVKDAVNHVIAHNKNTTHSL
>Mature_268_residues
MIKMFVSDIDGTMMQHGGIIDEQDVAALRSLAEQNVILCFASGRLDNEIADLMKAVNTNFHRISVNGVFVYTDENKQLLS
ATFDSSILPELLDMTNEDPYFRYVSDEHNYYIEEKTPFIHELEKQVTMTSVEEPNLLQKIDDTIFPNKISVGGTKESLQL
LQKKIDEKFHGKVSTFISAEQCLDVMPPNISKGSAISVLLKEFQLQPEEVACIGDSYNDIPMFSLTPHSFAMAQADDAVK
EHAHYVVNTVKDAVNHVIAHNKNTTHSL

Specific function: Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI1787043, Length=275, Percent_Identity=26.5454545454545, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI87081741, Length=273, Percent_Identity=22.7106227106227, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI48994981, Length=270, Percent_Identity=23.3333333333333, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: NA

Molecular weight: Translated: 30097; Mature: 30097

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKMFVSDIDGTMMQHGGIIDEQDVAALRSLAEQNVILCFASGRLDNEIADLMKAVNTNF
CCEEEEECCCCHHHHCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCC
HRISVNGVFVYTDENKQLLSATFDSSILPELLDMTNEDPYFRYVSDEHNYYIEEKTPFIH
EEEEEEEEEEEECCCCEEHHHHHCHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCHHH
ELEKQVTMTSVEEPNLLQKIDDTIFPNKISVGGTKESLQLLQKKIDEKFHGKVSTFISAE
HHHHHHHHHCCCCCHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
QCLDVMPPNISKGSAISVLLKEFQLQPEEVACIGDSYNDIPMFSLTPHSFAMAQADDAVK
HHHHHCCCCCCCCHHHHHHHHHHCCCHHHEEEECCCCCCCCEEEECCCHHHHHHHHHHHH
EHAHYVVNTVKDAVNHVIAHNKNTTHSL
HHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MIKMFVSDIDGTMMQHGGIIDEQDVAALRSLAEQNVILCFASGRLDNEIADLMKAVNTNF
CCEEEEECCCCHHHHCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCC
HRISVNGVFVYTDENKQLLSATFDSSILPELLDMTNEDPYFRYVSDEHNYYIEEKTPFIH
EEEEEEEEEEEECCCCEEHHHHHCHHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCHHH
ELEKQVTMTSVEEPNLLQKIDDTIFPNKISVGGTKESLQLLQKKIDEKFHGKVSTFISAE
HHHHHHHHHCCCCCHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
QCLDVMPPNISKGSAISVLLKEFQLQPEEVACIGDSYNDIPMFSLTPHSFAMAQADDAVK
HHHHHCCCCCCCCHHHHHHHHHHCCCHHHEEEECCCCCCCCEEEECCCHHHHHHHHHHHH
EHAHYVVNTVKDAVNHVIAHNKNTTHSL
HHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA