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The map label for this gene is sigF [H]

Identifier: 49186821

GI number: 49186821

Start: 3781041

End: 3781985

Strand: Reverse

Name: sigF [H]

Synonym: BAS3823

Alternate gene names: 49186821

Gene position: 3781985-3781041 (Counterclockwise)

Preceding gene: 49186822

Following gene: 49186820

Centisome position: 72.33

GC content: 34.6

Gene sequence:

>945_bases
GTGGGGGATGAACAAAAGGATTTGATTGAACAAGAAGATGTTCTAATGATGACTGATGATGATTTTATGAAAAAGTATAG
CAGGCTAGTTTATTACTATGTAAACAAATATTTTTCCAATATCGCTAAAGCAATTGAACAAGAAACAAATTTAAATATGG
ATGATTTAATTCAGTCTGGAATGTTGGGGTTGGTCACAGCGAGAAGAGATTTTAAAACAGAACTTGGGTTTAAATTTACG
ACATTCGCTGTTCCAAGAATACTTGGATATATAAGAAAGGCTGTTAGGGACTCGCAAAAAATTAGGATTACGAACGAAGT
GTATGAAATCAAAGGAAAGATTTCTAGAATGAAACTTCATGATGAAGAAGTGGAAAGTATTGCTAAATTACTAGAAGTTA
GCGTGAGAGATGTGAAGACAGCAAAAGAATTTAATCCTAGAACGATTTCACTTCAAACACCATTAAATAAAGAGGTAAAC
AAGGAGAAAGTCACTCTAGAAAATACGCTAATAGATAAAAAAACATCAATGACACTCGATGATGTTATTAAGAAAATGAT
AATCACAGCGTTCTTATCAACCTTATGTAGTAAAGAGAAGTTTGTATGGGAACTACATGCTAAAAATATGACACAAGCTG
CAATTGGCAAACGGTTAGGTGTAACGCAGACACAAATAAGTAGGATTTTAAAAGGGATTTATAAGAAAGCTGAAGAGTAT
GGAAATAAACATAGCTTACGTGATATAGGCAAGACAAGGTTTGAAAAAACAATTGAAAATCACGTGAGACAAAGTAGCCA
TGGGAATTGGTTGGATATAGCTGAAAGTAATGGAGTAAGTAGAGGAGCGTTTTGGTATAGATTGCGTGAAGGGTGGTCTT
ACGAGATGGCAGCCACACAACCATTAGGCAGTCAAGGGACAAGGAAGAATAAGCAAGTTATTTAG

Upstream 100 bases:

>100_bases
TGATTATGGATCAAGCTGATAAAAATAAAATCAAATCACGCCCAATGGGCCTAGGAGCATGAAAAAAGGAGTTATGAAAT
TAAATTAGGAGGAGAAAACG

Downstream 100 bases:

>100_bases
GAGGCAACATGGATAAGCAAAACATTTTGATTAATAAATTAATCGATAATCGTATATACAAGCTTCCAGACGGGCGTGAT
CTATTCGAGGGATCGATCGA

Product: prophage LambdaBa02, RNA polymerase sigma-F factor

Products: NA

Alternate protein names: Sporulation sigma factor; Stage II sporulation protein AC [H]

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MGDEQKDLIEQEDVLMMTDDDFMKKYSRLVYYYVNKYFSNIAKAIEQETNLNMDDLIQSGMLGLVTARRDFKTELGFKFT
TFAVPRILGYIRKAVRDSQKIRITNEVYEIKGKISRMKLHDEEVESIAKLLEVSVRDVKTAKEFNPRTISLQTPLNKEVN
KEKVTLENTLIDKKTSMTLDDVIKKMIITAFLSTLCSKEKFVWELHAKNMTQAAIGKRLGVTQTQISRILKGIYKKAEEY
GNKHSLRDIGKTRFEKTIENHVRQSSHGNWLDIAESNGVSRGAFWYRLREGWSYEMAATQPLGSQGTRKNKQVI

Sequences:

>Translated_314_residues
MGDEQKDLIEQEDVLMMTDDDFMKKYSRLVYYYVNKYFSNIAKAIEQETNLNMDDLIQSGMLGLVTARRDFKTELGFKFT
TFAVPRILGYIRKAVRDSQKIRITNEVYEIKGKISRMKLHDEEVESIAKLLEVSVRDVKTAKEFNPRTISLQTPLNKEVN
KEKVTLENTLIDKKTSMTLDDVIKKMIITAFLSTLCSKEKFVWELHAKNMTQAAIGKRLGVTQTQISRILKGIYKKAEEY
GNKHSLRDIGKTRFEKTIENHVRQSSHGNWLDIAESNGVSRGAFWYRLREGWSYEMAATQPLGSQGTRKNKQVI
>Mature_313_residues
GDEQKDLIEQEDVLMMTDDDFMKKYSRLVYYYVNKYFSNIAKAIEQETNLNMDDLIQSGMLGLVTARRDFKTELGFKFTT
FAVPRILGYIRKAVRDSQKIRITNEVYEIKGKISRMKLHDEEVESIAKLLEVSVRDVKTAKEFNPRTISLQTPLNKEVNK
EKVTLENTLIDKKTSMTLDDVIKKMIITAFLSTLCSKEKFVWELHAKNMTQAAIGKRLGVTQTQISRILKGIYKKAEEYG
NKHSLRDIGKTRFEKTIENHVRQSSHGNWLDIAESNGVSRGAFWYRLREGWSYEMAATQPLGSQGTRKNKQVI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of sporulation specific genes. Interaction with spoIIAB inhibits sig

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

None

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014322
- InterPro:   IPR014236
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36313; Mature: 36182

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDEQKDLIEQEDVLMMTDDDFMKKYSRLVYYYVNKYFSNIAKAIEQETNLNMDDLIQSG
CCCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
MLGLVTARRDFKTELGFKFTTFAVPRILGYIRKAVRDSQKIRITNEVYEIKGKISRMKLH
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCC
DEEVESIAKLLEVSVRDVKTAKEFNPRTISLQTPLNKEVNKEKVTLENTLIDKKTSMTLD
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHCCCHH
DVIKKMIITAFLSTLCSKEKFVWELHAKNMTQAAIGKRLGVTQTQISRILKGIYKKAEEY
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GNKHSLRDIGKTRFEKTIENHVRQSSHGNWLDIAESNGVSRGAFWYRLREGWSYEMAATQ
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHCCCCCCHHCCC
PLGSQGTRKNKQVI
CCCCCCCCCCCCCC
>Mature Secondary Structure 
GDEQKDLIEQEDVLMMTDDDFMKKYSRLVYYYVNKYFSNIAKAIEQETNLNMDDLIQSG
CCCHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCC
MLGLVTARRDFKTELGFKFTTFAVPRILGYIRKAVRDSQKIRITNEVYEIKGKISRMKLH
CHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCC
DEEVESIAKLLEVSVRDVKTAKEFNPRTISLQTPLNKEVNKEKVTLENTLIDKKTSMTLD
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCHHCCCHH
DVIKKMIITAFLSTLCSKEKFVWELHAKNMTQAAIGKRLGVTQTQISRILKGIYKKAEEY
HHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
GNKHSLRDIGKTRFEKTIENHVRQSSHGNWLDIAESNGVSRGAFWYRLREGWSYEMAATQ
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHCCCCCCHHCCC
PLGSQGTRKNKQVI
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3116160; 6088674; 8969508; 9384377; 8358793 [H]