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The map label for this gene is mrdA [H]

Identifier: 49185334

GI number: 49185334

Start: 2324062

End: 2325801

Strand: Reverse

Name: mrdA [H]

Synonym: BAS2325

Alternate gene names: 49185334

Gene position: 2325801-2324062 (Counterclockwise)

Preceding gene: 49185338

Following gene: 49185329

Centisome position: 44.48

GC content: 32.7

Gene sequence:

>1740_bases
ATGTTACATACAAAAATAAAAAAATGGGGATTTTTTGCGGTTCTTTCTATTGTAGGTTTGATTTTACTAATTTTATTTAA
AATCAACTTCATTTCAATTACTATCGCAACTTCTTCAGCCGAAAGAGGAAAAATCTTTGATCAAAATGGTGTAATACTAG
CTACAAATAAGAAAGTTAAGTCTCTATATTGTACTTCTAATGATGAAAAGTTATCGAAACAAGATACATCAAACACATTA
GCTTTTTTCGAGCAATTTTCAAGTAAGTTTCAACATGATATTTCTTCAGAGAGCATAAAATCTCAATTACAACAATCTTG
TAAAAAACAGACTATGCATGATATACCGTTATACTCTGATATAGCTGAGAATGAACTTGCATTCATAGACAAAAACAAAC
CGAATAATGTAATAATTAAAGATGAATGGATTCGATATTATCCTAAACATGAAATTGGTTCACAGGTAATTGGGTATGTA
GAAAATAACCTTAATTCAAAGCATATGCCGGTTGGAAAAAGTGGGATTGAGCTGCAATATGAAGATGATTTAAAAGGAAA
ACCGGGTAAAACTCTTATTTTTAAAATGAATAATAAAGAGTTCTTATGGAACATTCAAAAAGTACAAAAAGGTAAGGATA
TCAGGCTATCTTTAGATTCTAAATTGCAACAAAAATCAGAGGAAGCATTACGATCGCAAATTAAGAAATTACCTGATGCT
AAAGCTGGTTATGCTGTAGTGAGTGATGCAAAAACTGGCGCAATTTTAACTATGGCCAACTCAGCAGTTTTTGATCCAAA
TATATTACATACTCATGTATCAACTAAAATAGATAACTTAAAATCACTTTCGCAAAATAAAGCAATTCAAAAATTAAAGT
ATGGTGAATCTTATATAAATATGGCCTCTACTATAAAACCACTTACTATTTTAATTGGATTAAACGAAAAACTTTTTCAA
CCTGAAGATACTTATTTAGATAAAGGTACTTTTGAGTATGATAATCAGAATAACATTACAAATGCACCTGGAACGCCTAC
AGGTGAGATTACACCGAGGCAAGCTATCATTAATTCGTCTAACACATTTATGACAGCAAAAGTAGCTCTTCCATTATTTA
ACCAACATAATGGCAATGTAGAAAAAGTAGCACATATATGGACAGATTATTTAATGCAATTCGGCCTACGTTCTAAAACC
GGGATTGATTTACCCTTTGAAGAAGACGGACAATATGAATTCCATCCTTCCAATAAGTTTGAAAATGGAATCTCCGCTTT
ATTAAATGCTTCGTGGGGCGGAAACGAAGTGCATACTCCTCTTCAACTCGCTCAATATGCAGCAACTTTGGCAAGGAAAG
GTGATAAATATAAACCTCAAATGACAAGTGCTATTATTGGTCAAAATGGTAAGGAAACAAAAAAGTTTAAACCAATTTTA
GAAAGTTCAAATCGTTATCCGACGAAATTTTGGAGTGTCGTGCAAGGTGGAATGAGTCAAAATATAGAGGAGATTAAAAA
GTTGCCCTTTGACGTCGCTGGTAAAACAGGTATTACGGGTTTTCCGGATGAGCAAGCAAGAATGATAAACCATTCTCTAT
TCATTGCATATGCTCCTACCGAAGATCCACAGATTGCCGTTTCTGTCGTTATTCCTGGAAGTAATTCAGAAAAAAAACAT
TGCTGCTCTCGTTACATCAGAAATTTTAGAGTCCTGGAATACTCTTCAAAAAGAGAATAA

Upstream 100 bases:

>100_bases
CTGTAGTACTTAATAGACAATCTAAATAATAAAGTTGTTCCACATTTACAAAAAAAATAATTAACTATAAGATGAAGTAG
GCTACAAAAGGGAGGATTTT

Downstream 100 bases:

>100_bases
AAACAAAAAGGAAGGTTCATTAAAGTGAACCTTCCTTTTTTACCAAATAGAATCTATATTTATTATTTTCCATATACCTT
CATTATTCTTTTCAAACATA

Product: penicillin-binding protein

Products: NA

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MLHTKIKKWGFFAVLSIVGLILLILFKINFISITIATSSAERGKIFDQNGVILATNKKVKSLYCTSNDEKLSKQDTSNTL
AFFEQFSSKFQHDISSESIKSQLQQSCKKQTMHDIPLYSDIAENELAFIDKNKPNNVIIKDEWIRYYPKHEIGSQVIGYV
ENNLNSKHMPVGKSGIELQYEDDLKGKPGKTLIFKMNNKEFLWNIQKVQKGKDIRLSLDSKLQQKSEEALRSQIKKLPDA
KAGYAVVSDAKTGAILTMANSAVFDPNILHTHVSTKIDNLKSLSQNKAIQKLKYGESYINMASTIKPLTILIGLNEKLFQ
PEDTYLDKGTFEYDNQNNITNAPGTPTGEITPRQAIINSSNTFMTAKVALPLFNQHNGNVEKVAHIWTDYLMQFGLRSKT
GIDLPFEEDGQYEFHPSNKFENGISALLNASWGGNEVHTPLQLAQYAATLARKGDKYKPQMTSAIIGQNGKETKKFKPIL
ESSNRYPTKFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPDEQARMINHSLFIAYAPTEDPQIAVSVVIPGSNSEKKH
CCSRYIRNFRVLEYSSKRE

Sequences:

>Translated_579_residues
MLHTKIKKWGFFAVLSIVGLILLILFKINFISITIATSSAERGKIFDQNGVILATNKKVKSLYCTSNDEKLSKQDTSNTL
AFFEQFSSKFQHDISSESIKSQLQQSCKKQTMHDIPLYSDIAENELAFIDKNKPNNVIIKDEWIRYYPKHEIGSQVIGYV
ENNLNSKHMPVGKSGIELQYEDDLKGKPGKTLIFKMNNKEFLWNIQKVQKGKDIRLSLDSKLQQKSEEALRSQIKKLPDA
KAGYAVVSDAKTGAILTMANSAVFDPNILHTHVSTKIDNLKSLSQNKAIQKLKYGESYINMASTIKPLTILIGLNEKLFQ
PEDTYLDKGTFEYDNQNNITNAPGTPTGEITPRQAIINSSNTFMTAKVALPLFNQHNGNVEKVAHIWTDYLMQFGLRSKT
GIDLPFEEDGQYEFHPSNKFENGISALLNASWGGNEVHTPLQLAQYAATLARKGDKYKPQMTSAIIGQNGKETKKFKPIL
ESSNRYPTKFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPDEQARMINHSLFIAYAPTEDPQIAVSVVIPGSNSEKKH
CCSRYIRNFRVLEYSSKRE
>Mature_579_residues
MLHTKIKKWGFFAVLSIVGLILLILFKINFISITIATSSAERGKIFDQNGVILATNKKVKSLYCTSNDEKLSKQDTSNTL
AFFEQFSSKFQHDISSESIKSQLQQSCKKQTMHDIPLYSDIAENELAFIDKNKPNNVIIKDEWIRYYPKHEIGSQVIGYV
ENNLNSKHMPVGKSGIELQYEDDLKGKPGKTLIFKMNNKEFLWNIQKVQKGKDIRLSLDSKLQQKSEEALRSQIKKLPDA
KAGYAVVSDAKTGAILTMANSAVFDPNILHTHVSTKIDNLKSLSQNKAIQKLKYGESYINMASTIKPLTILIGLNEKLFQ
PEDTYLDKGTFEYDNQNNITNAPGTPTGEITPRQAIINSSNTFMTAKVALPLFNQHNGNVEKVAHIWTDYLMQFGLRSKT
GIDLPFEEDGQYEFHPSNKFENGISALLNASWGGNEVHTPLQLAQYAATLARKGDKYKPQMTSAIIGQNGKETKKFKPIL
ESSNRYPTKFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPDEQARMINHSLFIAYAPTEDPQIAVSVVIPGSNSEKKH
CCSRYIRNFRVLEYSSKRE

Specific function: Cell wall formation; PBP-2 is responsible for the determination of the rod shape of the cell. Its synthesize cross- linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786854, Length=611, Percent_Identity=21.1129296235679, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1786272, Length=542, Percent_Identity=24.3542435424354, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460
- InterPro:   IPR017790 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: NA

Molecular weight: Translated: 65160; Mature: 65160

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLHTKIKKWGFFAVLSIVGLILLILFKINFISITIATSSAERGKIFDQNGVILATNKKVK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCEECCCCEEEEECCCEE
SLYCTSNDEKLSKQDTSNTLAFFEQFSSKFQHDISSESIKSQLQQSCKKQTMHDIPLYSD
EEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHH
IAENELAFIDKNKPNNVIIKDEWIRYYPKHEIGSQVIGYVENNLNSKHMPVGKSGIELQY
HCCCCEEEEECCCCCEEEEECHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
EDDLKGKPGKTLIFKMNNKEFLWNIQKVQKGKDIRLSLDSKLQQKSEEALRSQIKKLPDA
CCCCCCCCCCEEEEEECCCCEEEEHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCC
KAGYAVVSDAKTGAILTMANSAVFDPNILHTHVSTKIDNLKSLSQNKAIQKLKYGESYIN
CCCEEEEECCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
MASTIKPLTILIGLNEKLFQPEDTYLDKGTFEYDNQNNITNAPGTPTGEITPRQAIINSS
HHHCCCEEEEEEECCHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCC
NTFMTAKVALPLFNQHNGNVEKVAHIWTDYLMQFGLRSKTGIDLPFEEDGQYEFHPSNKF
CCEEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHH
ENGISALLNASWGGNEVHTPLQLAQYAATLARKGDKYKPQMTSAIIGQNGKETKKFKPIL
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHH
ESSNRYPTKFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPDEQARMINHSLFIAYAPT
HCCCCCCHHHHHHHHCHHHCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCEEEEEECCC
EDPQIAVSVVIPGSNSEKKHCCSRYIRNFRVLEYSSKRE
CCCCEEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCC
>Mature Secondary Structure
MLHTKIKKWGFFAVLSIVGLILLILFKINFISITIATSSAERGKIFDQNGVILATNKKVK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCEECCCCEEEEECCCEE
SLYCTSNDEKLSKQDTSNTLAFFEQFSSKFQHDISSESIKSQLQQSCKKQTMHDIPLYSD
EEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHH
IAENELAFIDKNKPNNVIIKDEWIRYYPKHEIGSQVIGYVENNLNSKHMPVGKSGIELQY
HCCCCEEEEECCCCCEEEEECHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEE
EDDLKGKPGKTLIFKMNNKEFLWNIQKVQKGKDIRLSLDSKLQQKSEEALRSQIKKLPDA
CCCCCCCCCCEEEEEECCCCEEEEHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCC
KAGYAVVSDAKTGAILTMANSAVFDPNILHTHVSTKIDNLKSLSQNKAIQKLKYGESYIN
CCCEEEEECCCCCEEEEEECCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCHHHHH
MASTIKPLTILIGLNEKLFQPEDTYLDKGTFEYDNQNNITNAPGTPTGEITPRQAIINSS
HHHCCCEEEEEEECCHHHCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHCCCC
NTFMTAKVALPLFNQHNGNVEKVAHIWTDYLMQFGLRSKTGIDLPFEEDGQYEFHPSNKF
CCEEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCHH
ENGISALLNASWGGNEVHTPLQLAQYAATLARKGDKYKPQMTSAIIGQNGKETKKFKPIL
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHH
ESSNRYPTKFWSVVQGGMSQNIEEIKKLPFDVAGKTGITGFPDEQARMINHSLFIAYAPT
HCCCCCCHHHHHHHHCHHHCCHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCEEEEEECCC
EDPQIAVSVVIPGSNSEKKHCCSRYIRNFRVLEYSSKRE
CCCCEEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]