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The map label for this gene is ywjE [H]

Identifier: 49184919

GI number: 49184919

Start: 1927201

End: 1928337

Strand: Direct

Name: ywjE [H]

Synonym: BAS1907

Alternate gene names: 49184919

Gene position: 1927201-1928337 (Clockwise)

Preceding gene: 49184918

Following gene: 49184920

Centisome position: 36.86

GC content: 34.39

Gene sequence:

>1137_bases
TTGGATGAACATCGATGTGGAAATGGGGAAAAAATGGCTAGTGAATATGAATTAGGACAAGTTCGATTGGGTGAATTTCA
ACTGTATACGAACGGTGAAGAATTATATACGAAATTATTTGAGGATATACGCAATGCAGAAAAGTACATATATATCCATT
TTTATATTGTAGGTAAAGACGAAATAAGCAAAGAGTTTTTGCAGTTGCTCGAGAAAAAGGCATCAGGTGGAGTAGAAGTG
AAATTGTCAGTCGATCGAATAGGTGGATATAAGCTGAAGAAAAAGATAATTCGTCAACTAAAAAGCAATGGTGTCCAGTT
TACATTTAGTAAAAAACCTAAACTGAAACATATATTCTACTCGTTGCATCAACGGAACCATAGACGCATTGTTACGATAG
ATGGAAAAGTATCATACGTCGGCGGCTTTAACATTGGAAAGGAATACCTTGGACAAAATCCGAAATTTGGTCCGTGGCGT
GATTATCATGTACGAGTCAAGGGGAATGGTGCCACGGATATGGAAAGAAAATTCTCTGCTGACTGGAAAGAAGATACTGG
AGAAAAAATGCCTGTACATGAAAGTTTACCTGCAATAGGGAATGTAAAGTATCAATATTTATTTTCAGAAGGAAAAGGTT
TATGGGAAAAATATGGAGCGCTTATAAAGCAGGCTAAAACGTCTTTAATTATTGCTACACCATATTTTGTACCAAGTAAA
GAAATGATAAAAGCGTTAAAAGATGCATTAAACCGTGGTGTCAATGTAAAAATTCTCGTACCATTTAAAAGTGATGCAGT
ATTGTTAAAACAAGCTGCCTATCCGTATTTGAAAGACATGTTACATGCTGGAGCAGAGATTTATCAATATCAGAATGGTT
TTTTTCATGGGAAAGTAACAATCATTGATGGGGAAAGTGTTGATATTGGTACCGCAAACTTTGATAATAGAAGTTTTTAT
TTAAATATGGAATCCAATTGTGTTATCTATGATAAGGGAGTAACAGCGGACGTATGGAGTCGATTAAAAGAAGACTTTCA
TAAATCAAAAAGATTTTCGGAAGAAGATTTTGAGAAAATTAGTAAATGGGATTGGTTTTTAGCAAGAATAGCAAATGTTA
TAGCATCATATTTATAG

Upstream 100 bases:

>100_bases
ATTAAAAAAGGGAAGATATAGAAATAACAGTAAGTGGAGAAAGGAAAATATCCATGAAACACTTTTTCGTTGTAATCCTT
TGTTTAATAGGTGTATTCAT

Downstream 100 bases:

>100_bases
CATATAGACAAAAAAGAAGGGAGGCACTTTCATGGATTGTATGAAAGAATTAATGAGGTATAGTTATATGCTTATATATA
AGGTTGGGGAGTATACAGGA

Product: cardiolipin synthetase domain-containing protein

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MDEHRCGNGEKMASEYELGQVRLGEFQLYTNGEELYTKLFEDIRNAEKYIYIHFYIVGKDEISKEFLQLLEKKASGGVEV
KLSVDRIGGYKLKKKIIRQLKSNGVQFTFSKKPKLKHIFYSLHQRNHRRIVTIDGKVSYVGGFNIGKEYLGQNPKFGPWR
DYHVRVKGNGATDMERKFSADWKEDTGEKMPVHESLPAIGNVKYQYLFSEGKGLWEKYGALIKQAKTSLIIATPYFVPSK
EMIKALKDALNRGVNVKILVPFKSDAVLLKQAAYPYLKDMLHAGAEIYQYQNGFFHGKVTIIDGESVDIGTANFDNRSFY
LNMESNCVIYDKGVTADVWSRLKEDFHKSKRFSEEDFEKISKWDWFLARIANVIASYL

Sequences:

>Translated_378_residues
MDEHRCGNGEKMASEYELGQVRLGEFQLYTNGEELYTKLFEDIRNAEKYIYIHFYIVGKDEISKEFLQLLEKKASGGVEV
KLSVDRIGGYKLKKKIIRQLKSNGVQFTFSKKPKLKHIFYSLHQRNHRRIVTIDGKVSYVGGFNIGKEYLGQNPKFGPWR
DYHVRVKGNGATDMERKFSADWKEDTGEKMPVHESLPAIGNVKYQYLFSEGKGLWEKYGALIKQAKTSLIIATPYFVPSK
EMIKALKDALNRGVNVKILVPFKSDAVLLKQAAYPYLKDMLHAGAEIYQYQNGFFHGKVTIIDGESVDIGTANFDNRSFY
LNMESNCVIYDKGVTADVWSRLKEDFHKSKRFSEEDFEKISKWDWFLARIANVIASYL
>Mature_378_residues
MDEHRCGNGEKMASEYELGQVRLGEFQLYTNGEELYTKLFEDIRNAEKYIYIHFYIVGKDEISKEFLQLLEKKASGGVEV
KLSVDRIGGYKLKKKIIRQLKSNGVQFTFSKKPKLKHIFYSLHQRNHRRIVTIDGKVSYVGGFNIGKEYLGQNPKFGPWR
DYHVRVKGNGATDMERKFSADWKEDTGEKMPVHESLPAIGNVKYQYLFSEGKGLWEKYGALIKQAKTSLIIATPYFVPSK
EMIKALKDALNRGVNVKILVPFKSDAVLLKQAAYPYLKDMLHAGAEIYQYQNGFFHGKVTIIDGESVDIGTANFDNRSFY
LNMESNCVIYDKGVTADVWSRLKEDFHKSKRFSEEDFEKISKWDWFLARIANVIASYL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=367, Percent_Identity=29.4277929155313, Blast_Score=171, Evalue=5e-44,
Organism=Escherichia coli, GI1787007, Length=329, Percent_Identity=26.1398176291793, Blast_Score=131, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 43596; Mature: 43596

Theoretical pI: Translated: 9.62; Mature: 9.62

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEHRCGNGEKMASEYELGQVRLGEFQLYTNGEELYTKLFEDIRNAEKYIYIHFYIVGKD
CCCCCCCCCHHHHHHHCCCCEEECEEEEEECHHHHHHHHHHHHCCCCEEEEEEEEEECCH
EISKEFLQLLEKKASGGVEVKLSVDRIGGYKLKKKIIRQLKSNGVQFTFSKKPKLKHIFY
HHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHH
SLHQRNHRRIVTIDGKVSYVGGFNIGKEYLGQNPKFGPWRDYHVRVKGNGATDMERKFSA
HHHCCCCCEEEEECCEEEEECCCCCCHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHCC
DWKEDTGEKMPVHESLPAIGNVKYQYLFSEGKGLWEKYGALIKQAKTSLIIATPYFVPSK
CCCCCCCCCCCCHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCH
EMIKALKDALNRGVNVKILVPFKSDAVLLKQAAYPYLKDMLHAGAEIYQYQNGFFHGKVT
HHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHCCHHHHHHHHCCHHHHHHCCCEEEEEEE
IIDGESVDIGTANFDNRSFYLNMESNCVIYDKGVTADVWSRLKEDFHKSKRFSEEDFEKI
EECCCCEEECCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHH
SKWDWFLARIANVIASYL
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDEHRCGNGEKMASEYELGQVRLGEFQLYTNGEELYTKLFEDIRNAEKYIYIHFYIVGKD
CCCCCCCCCHHHHHHHCCCCEEECEEEEEECHHHHHHHHHHHHCCCCEEEEEEEEEECCH
EISKEFLQLLEKKASGGVEVKLSVDRIGGYKLKKKIIRQLKSNGVQFTFSKKPKLKHIFY
HHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCEEEEECCCCCHHHHHH
SLHQRNHRRIVTIDGKVSYVGGFNIGKEYLGQNPKFGPWRDYHVRVKGNGATDMERKFSA
HHHCCCCCEEEEECCEEEEECCCCCCHHHCCCCCCCCCCCCEEEEEECCCCCCHHHHHCC
DWKEDTGEKMPVHESLPAIGNVKYQYLFSEGKGLWEKYGALIKQAKTSLIIATPYFVPSK
CCCCCCCCCCCCHHCCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCCCH
EMIKALKDALNRGVNVKILVPFKSDAVLLKQAAYPYLKDMLHAGAEIYQYQNGFFHGKVT
HHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHCCHHHHHHHHCCHHHHHHCCCEEEEEEE
IIDGESVDIGTANFDNRSFYLNMESNCVIYDKGVTADVWSRLKEDFHKSKRFSEEDFEKI
EECCCCEEECCCCCCCCEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHH
SKWDWFLARIANVIASYL
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377 [H]