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The map label for this gene is vanS [H]

Identifier: 49184753

GI number: 49184753

Start: 1760596

End: 1761276

Strand: Direct

Name: vanS [H]

Synonym: BAS1741

Alternate gene names: 49184753

Gene position: 1760596-1761276 (Clockwise)

Preceding gene: 49184752

Following gene: 49184754

Centisome position: 33.67

GC content: 32.75

Gene sequence:

>681_bases
ATGAAAAATGAAATGATTAGTAATATTTCGCATGACTTACGAACACCAGTAACATCTTTAATCGGTTACGCGGACTTGTT
AGGAAATAAGCTGCATTCAAATGGAGAAGAATGTGAACAGTATGTAAGTATATTAAAGCGGAAAAGTTATGAATTAAAAA
ATCAAGTAGATGATTTATTAGAATACTGTCAAATAAATTATAGAGAGATTGAATTACAAAAAAGTGTAGTAAATATGAAA
GCGTTCATTGAACAAATCATGATTGATTTTGTACCACAACTAGATGATGCCGATATGAGCTTTTGCATTAAAGGTGATAA
GGGGTTACATGTGGAAATGGATGTAGCGCTTATGGTGAGGCTATTCGAAAATGTAATTAGTAATAGTATTATGTACGGAA
AAGACGGAAAACAGATTTGGATTCAAATTTCTAAAAGAGACATGAATGTTGAAATAGAAATTAAAAATTTTGGGCAATGT
ATTCCGAATGAGAACCTCCCTTACGTTTTTGAGAAGTTTTATCGTGGTGAAAAATCACGCAGCTCTCATACAGGTGGAAA
AGGAATGGGACTTGCAATTGCGAGAAGTATAGCAGAACTTCATAAAGGAGATATCGCTGTACGTAGTAACGATAAAGAAA
CAGTTTTTACTATCGTTTTACCGCAGTATAAAGAAATGTAA

Upstream 100 bases:

>100_bases
CTATTTCAAAAAATGACGAGATTGGTGAATTAGCGAAGAATGTAAATGGAATGGCCAAAAGTTTAAAAGAGTCTATCGAG
AATGAAAAAAATCGCAGGAA

Downstream 100 bases:

>100_bases
GAAAATCGTAAGTATTTCTTCTGTATGTCGTAATTTTCACTTCGTATCATGTAGAACAAAGATATGAGAGTGGGGATATA
ACATGTGGAAGAAATATGTG

Product: sensor histidine kinase, C-terminus

Products: NA

Alternate protein names: Vancomycin histidine protein kinase; Vancomycin resistance protein vanS [H]

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MKNEMISNISHDLRTPVTSLIGYADLLGNKLHSNGEECEQYVSILKRKSYELKNQVDDLLEYCQINYREIELQKSVVNMK
AFIEQIMIDFVPQLDDADMSFCIKGDKGLHVEMDVALMVRLFENVISNSIMYGKDGKQIWIQISKRDMNVEIEIKNFGQC
IPNENLPYVFEKFYRGEKSRSSHTGGKGMGLAIARSIAELHKGDIAVRSNDKETVFTIVLPQYKEM

Sequences:

>Translated_226_residues
MKNEMISNISHDLRTPVTSLIGYADLLGNKLHSNGEECEQYVSILKRKSYELKNQVDDLLEYCQINYREIELQKSVVNMK
AFIEQIMIDFVPQLDDADMSFCIKGDKGLHVEMDVALMVRLFENVISNSIMYGKDGKQIWIQISKRDMNVEIEIKNFGQC
IPNENLPYVFEKFYRGEKSRSSHTGGKGMGLAIARSIAELHKGDIAVRSNDKETVFTIVLPQYKEM
>Mature_226_residues
MKNEMISNISHDLRTPVTSLIGYADLLGNKLHSNGEECEQYVSILKRKSYELKNQVDDLLEYCQINYREIELQKSVVNMK
AFIEQIMIDFVPQLDDADMSFCIKGDKGLHVEMDVALMVRLFENVISNSIMYGKDGKQIWIQISKRDMNVEIEIKNFGQC
IPNENLPYVFEKFYRGEKSRSSHTGGKGMGLAIARSIAELHKGDIAVRSNDKETVFTIVLPQYKEM

Specific function: Member of the two-component regulatory system vanS/vanR. Activates the transcription of vanH, vanA and vanX in response to vancomycin which results in vancomycin resistance. VanS activates vanR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=215, Percent_Identity=30.2325581395349, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1786600, Length=222, Percent_Identity=25.6756756756757, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1788393, Length=227, Percent_Identity=27.3127753303965, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1786912, Length=223, Percent_Identity=26.9058295964126, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87082128, Length=235, Percent_Identity=24.6808510638298, Blast_Score=81, Evalue=7e-17,
Organism=Escherichia coli, GI48994928, Length=228, Percent_Identity=26.7543859649123, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788279, Length=221, Percent_Identity=28.5067873303167, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1789149, Length=217, Percent_Identity=23.963133640553, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI145693157, Length=225, Percent_Identity=23.5555555555556, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI1787894, Length=205, Percent_Identity=27.8048780487805, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788549, Length=221, Percent_Identity=23.0769230769231, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790346, Length=227, Percent_Identity=22.9074889867841, Blast_Score=61, Evalue=5e-11,
Organism=Escherichia coli, GI1788713, Length=227, Percent_Identity=21.1453744493392, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 25941; Mature: 25941

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNEMISNISHDLRTPVTSLIGYADLLGNKLHSNGEECEQYVSILKRKSYELKNQVDDLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EYCQINYREIELQKSVVNMKAFIEQIMIDFVPQLDDADMSFCIKGDKGLHVEMDVALMVR
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEHHHHHHHH
LFENVISNSIMYGKDGKQIWIQISKRDMNVEIEIKNFGQCIPNENLPYVFEKFYRGEKSR
HHHHHHHCCEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCC
SSHTGGKGMGLAIARSIAELHKGDIAVRSNDKETVFTIVLPQYKEM
CCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCHHCCC
>Mature Secondary Structure
MKNEMISNISHDLRTPVTSLIGYADLLGNKLHSNGEECEQYVSILKRKSYELKNQVDDLL
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EYCQINYREIELQKSVVNMKAFIEQIMIDFVPQLDDADMSFCIKGDKGLHVEMDVALMVR
HHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEHHHHHHHH
LFENVISNSIMYGKDGKQIWIQISKRDMNVEIEIKNFGQCIPNENLPYVFEKFYRGEKSR
HHHHHHHCCEEECCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHCCCCCC
SSHTGGKGMGLAIARSIAELHKGDIAVRSNDKETVFTIVLPQYKEM
CCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCEEEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8380148; 1556077 [H]