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The map label for this gene is sap [H]

Identifier: 49184696

GI number: 49184696

Start: 1700901

End: 1702145

Strand: Direct

Name: sap [H]

Synonym: BAS1682

Alternate gene names: 49184696

Gene position: 1700901-1702145 (Clockwise)

Preceding gene: 49184694

Following gene: 49184697

Centisome position: 32.53

GC content: 35.66

Gene sequence:

>1245_bases
ATGAAAAATAGAATAATCGCAGCAGGAGTAATCGCAGCTAGTATATTATCTTATTCATCTAGTAGTTTCGCACAAACAAA
AACGTTTCCAGATGTCCAAACAGGGCACTGGGCAGAAGATTCTATTAACTACTTAGCAGAAAAAGGCGCAGTTACAGGTA
ACGAGAAAGGAATGTTCGAGCCTGGAAAGGAAATCACTCGTGCAGAAGCAGCTACAATGATGGCTAAAATCTTAAACTTG
CCAATCGATAAAAATGCTAAACCATCTTACGCTGATTCTCAAAAACATTGGGCTACTCCAATCATCGCAGCTGTAGAAAA
AGCTGGTGTTGTTAAAGGTACAGGCAATGGATTTGAGCCAGACGGAAAAATCGACCGTGTGTCAATGGCATCTCTTCTTG
TAGAAGCTTACAAATTAGAGTCTAAAGTAAACGGTACTCCAGCAACTAAATTCAAAGATTTAGAAACATTAAACTGGGGT
AAAGAAAAAGCTAATATTTTAGTTGAATTGGGTATTTCTGTTGGTACCGGTGACAAATGGGAACCTAAGAAAATTCTAAC
TAAAGCAGAAGCAGCTCAATTTATTAAAAAGGCAGATTCGCTTAAAGTAGGTAATCCTTTAGTAGAAAAGGTAGTTATTA
TTGATCCTGGTCATGGAGGATTTGATCCAGGGAATCCAGGACAAGGTGTAGAGGAAAGTAAAATTGTATTTGATACATCT
TTAAGATTACAGAAGTTGCTAGAAAAAAATACGCCTTTAAAAGCTTTGTTAACACGTGAAGAGAACGGAAATCCAGGAAG
TAACAAAAATGAATCTCTTGCGAATCGAGTGAAATTTGGTCAAGAGAACAATGCAGATATATTTGTAAGCATTCATGCAA
ATTCTTCTGAAAAACATGATGGTCATGGAACGGAAACGTATTATTATAAAAAATCAAAGCGTGGAGAAGAGACACAGATT
GAAAAGGATAGCGAAGTATTAGCGAAGAAAATTCAAAATAGAGTAGTAGAAGCACTACACACAAGAGATAGAAAGATAAA
AGACGATCACTCACTTTATGTAGTAAATAATAATACAGTACCAGCAGTTTTAACAGAGCTTGCCTTTATTGATAATGATA
TAGATAATGGTAAATTAGCTACTGAATCAGGAAGACAAATTGCTGCTGAGGCAGTTTATGCTGGGATTTTAGATTATTAT
GAATGGAAAGGTTTTGATGTTTCCAAATATCGTTTAGCTAAATAA

Upstream 100 bases:

>100_bases
AATACAAAATTATACAAATTGCAATTGACTATTACTAATTTTCAGAACTATAATTAGTCTGTTATATAAATACATTGTAC
TTGAGAGGGGAATATATTCT

Downstream 100 bases:

>100_bases
CAATGTTGATTGCTAAGCCCATCGTCTACATAGAGGTGGGCATTTTGTAAGTTTATGTAATTTTATATTGGAATAAAAAG
ATATATCGTATTATGCTGAT

Product: N-acetylmuramoyl-L-alanine amidase

Products: Hydrolyzed cell wall glycopeptides; N-acetylmuramoyl residues; L-amino acid residues [C]

Alternate protein names: Surface array protein; Surface layer protein [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKNRIIAAGVIAASILSYSSSSFAQTKTFPDVQTGHWAEDSINYLAEKGAVTGNEKGMFEPGKEITRAEAATMMAKILNL
PIDKNAKPSYADSQKHWATPIIAAVEKAGVVKGTGNGFEPDGKIDRVSMASLLVEAYKLESKVNGTPATKFKDLETLNWG
KEKANILVELGISVGTGDKWEPKKILTKAEAAQFIKKADSLKVGNPLVEKVVIIDPGHGGFDPGNPGQGVEESKIVFDTS
LRLQKLLEKNTPLKALLTREENGNPGSNKNESLANRVKFGQENNADIFVSIHANSSEKHDGHGTETYYYKKSKRGEETQI
EKDSEVLAKKIQNRVVEALHTRDRKIKDDHSLYVVNNNTVPAVLTELAFIDNDIDNGKLATESGRQIAAEAVYAGILDYY
EWKGFDVSKYRLAK

Sequences:

>Translated_414_residues
MKNRIIAAGVIAASILSYSSSSFAQTKTFPDVQTGHWAEDSINYLAEKGAVTGNEKGMFEPGKEITRAEAATMMAKILNL
PIDKNAKPSYADSQKHWATPIIAAVEKAGVVKGTGNGFEPDGKIDRVSMASLLVEAYKLESKVNGTPATKFKDLETLNWG
KEKANILVELGISVGTGDKWEPKKILTKAEAAQFIKKADSLKVGNPLVEKVVIIDPGHGGFDPGNPGQGVEESKIVFDTS
LRLQKLLEKNTPLKALLTREENGNPGSNKNESLANRVKFGQENNADIFVSIHANSSEKHDGHGTETYYYKKSKRGEETQI
EKDSEVLAKKIQNRVVEALHTRDRKIKDDHSLYVVNNNTVPAVLTELAFIDNDIDNGKLATESGRQIAAEAVYAGILDYY
EWKGFDVSKYRLAK
>Mature_414_residues
MKNRIIAAGVIAASILSYSSSSFAQTKTFPDVQTGHWAEDSINYLAEKGAVTGNEKGMFEPGKEITRAEAATMMAKILNL
PIDKNAKPSYADSQKHWATPIIAAVEKAGVVKGTGNGFEPDGKIDRVSMASLLVEAYKLESKVNGTPATKFKDLETLNWG
KEKANILVELGISVGTGDKWEPKKILTKAEAAQFIKKADSLKVGNPLVEKVVIIDPGHGGFDPGNPGQGVEESKIVFDTS
LRLQKLLEKNTPLKALLTREENGNPGSNKNESLANRVKFGQENNADIFVSIHANSSEKHDGHGTETYYYKKSKRGEETQI
EKDSEVLAKKIQNRVVEALHTRDRKIKDDHSLYVVNNNTVPAVLTELAFIDNDIDNGKLATESGRQIAAEAVYAGILDYY
EWKGFDVSKYRLAK

Specific function: The S-layer is a paracrystalline mono-layered assembly of proteins which coat the surface of bacteria [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall, S-layer [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 SLH (S-layer homology) domains [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=269, Percent_Identity=27.1375464684015, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003343
- InterPro:   IPR008964
- InterPro:   IPR001119 [H]

Pfam domain/function: PF02368 Big_2; PF00395 SLH [H]

EC number: 3.5.1.28 [C]

Molecular weight: Translated: 45352; Mature: 45352

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNRIIAAGVIAASILSYSSSSFAQTKTFPDVQTGHWAEDSINYLAEKGAVTGNEKGMFE
CCCCEEEHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
PGKEITRAEAATMMAKILNLPIDKNAKPSYADSQKHWATPIIAAVEKAGVVKGTGNGFEP
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEECCCCCCCC
DGKIDRVSMASLLVEAYKLESKVNGTPATKFKDLETLNWGKEKANILVELGISVGTGDKW
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCHHCCEEEEECCEECCCCCC
EPKKILTKAEAAQFIKKADSLKVGNPLVEKVVIIDPGHGGFDPGNPGQGVEESKIVFDTS
CHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEHH
LRLQKLLEKNTPLKALLTREENGNPGSNKNESLANRVKFGQENNADIFVSIHANSSEKHD
HHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
GHGTETYYYKKSKRGEETQIEKDSEVLAKKIQNRVVEALHTRDRKIKDDHSLYVVNNNTV
CCCCCEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCH
PAVLTELAFIDNDIDNGKLATESGRQIAAEAVYAGILDYYEWKGFDVSKYRLAK
HHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC
>Mature Secondary Structure
MKNRIIAAGVIAASILSYSSSSFAQTKTFPDVQTGHWAEDSINYLAEKGAVTGNEKGMFE
CCCCEEEHHHHHHHHHHHCCCCCHHCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
PGKEITRAEAATMMAKILNLPIDKNAKPSYADSQKHWATPIIAAVEKAGVVKGTGNGFEP
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHCCHHHHHHHHCCCEECCCCCCCC
DGKIDRVSMASLLVEAYKLESKVNGTPATKFKDLETLNWGKEKANILVELGISVGTGDKW
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCHHCCEEEEECCEECCCCCC
EPKKILTKAEAAQFIKKADSLKVGNPLVEKVVIIDPGHGGFDPGNPGQGVEESKIVFDTS
CHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCEEEEEHH
LRLQKLLEKNTPLKALLTREENGNPGSNKNESLANRVKFGQENNADIFVSIHANSSEKHD
HHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
GHGTETYYYKKSKRGEETQIEKDSEVLAKKIQNRVVEALHTRDRKIKDDHSLYVVNNNTV
CCCCCEEEEECCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCH
PAVLTELAFIDNDIDNGKLATESGRQIAAEAVYAGILDYYEWKGFDVSKYRLAK
HHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: cell wall glycopeptides; D-lactyl-L-Ala [C]

Specific reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836294; 12721629 [H]