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The map label for this gene is yfiK [H]

Identifier: 49184673

GI number: 49184673

Start: 1673857

End: 1674495

Strand: Direct

Name: yfiK [H]

Synonym: BAS1659

Alternate gene names: 49184673

Gene position: 1673857-1674495 (Clockwise)

Preceding gene: 49184668

Following gene: 49184674

Centisome position: 32.01

GC content: 32.24

Gene sequence:

>639_bases
ATGAAAATTAAAATATTAATAGCGGATGATAACTCTTTTATAAGAGAAGGTATGAAAATTATTTTAAATACATATGAAGA
GTTTGAAGTGGTAGATACAGTAAATGATGGGAAAGAAGCAGTGGAATATTGTAAAAAGAACGACGTTGATATAGCGCTTT
TAGACGTTCGTATGCCAAATATGAACGGGGTAGAGGCAACGAAATTGATTTGTGAAGAGACAGAAACGAAACCGCTTATT
TTAACGACGTTCGATGATGATGAATATATTTTGGACGCAGTAAAAAACGGAGCGAAAGGTTATTTATTAAAAAATAATGA
TCCAGAGCGTATTCGTGATGCGATAAAAGGAGTATATAACGGTCAAACTGTTATGCAAGATGTAGTGCTTGATAAAATTA
AGTGCAATTTAATGGAAAGTAAAGAGGACGAATGTAAAATAGATAAGAGTCTTTTTACAGAGAGAGAGCTTAGTATTATC
GCGTTAATAGCGAAAGGTTTCTCGAATAAAGAAATTTCAAAGCAACTTTTCATATCAGAAGGAACAATCGCAAATTATAT
TACATCAGTTTTAGGGAAAACTGGACTTGAACATCGTACACAAATTGCGATTTATTATTTAACAGGGAAAGTAGATTAA

Upstream 100 bases:

>100_bases
TGTGAAAAAATGGTAAAAAGAAGTATATAATGAGCAAGTGGGAAATGTTGCACAGAGAAAGGATGTGAATGTTAGTAGGG
GGAGTTCCATACTAACAAAC

Downstream 100 bases:

>100_bases
TGTTATGGAATTTTGGTTAACTGTAAGTAAATTAATTGTCTTTATATATATTGTGTTCAGTTACATTTATTTAAATGTTG
TGAACTTACCGTGGGTTATA

Product: LuxR family DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKIKILIADDNSFIREGMKIILNTYEEFEVVDTVNDGKEAVEYCKKNDVDIALLDVRMPNMNGVEATKLICEETETKPLI
LTTFDDDEYILDAVKNGAKGYLLKNNDPERIRDAIKGVYNGQTVMQDVVLDKIKCNLMESKEDECKIDKSLFTERELSII
ALIAKGFSNKEISKQLFISEGTIANYITSVLGKTGLEHRTQIAIYYLTGKVD

Sequences:

>Translated_212_residues
MKIKILIADDNSFIREGMKIILNTYEEFEVVDTVNDGKEAVEYCKKNDVDIALLDVRMPNMNGVEATKLICEETETKPLI
LTTFDDDEYILDAVKNGAKGYLLKNNDPERIRDAIKGVYNGQTVMQDVVLDKIKCNLMESKEDECKIDKSLFTERELSII
ALIAKGFSNKEISKQLFISEGTIANYITSVLGKTGLEHRTQIAIYYLTGKVD
>Mature_212_residues
MKIKILIADDNSFIREGMKIILNTYEEFEVVDTVNDGKEAVEYCKKNDVDIALLDVRMPNMNGVEATKLICEETETKPLI
LTTFDDDEYILDAVKNGAKGYLLKNNDPERIRDAIKGVYNGQTVMQDVVLDKIKCNLMESKEDECKIDKSLFTERELSII
ALIAKGFSNKEISKQLFISEGTIANYITSVLGKTGLEHRTQIAIYYLTGKVD

Specific function: Member of the two-component regulatory system yfiJ/yfiK [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=185, Percent_Identity=32.972972972973, Blast_Score=114, Evalue=5e-27,
Organism=Escherichia coli, GI1788521, Length=208, Percent_Identity=31.25, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787473, Length=202, Percent_Identity=29.7029702970297, Blast_Score=90, Evalue=9e-20,
Organism=Escherichia coli, GI1786747, Length=197, Percent_Identity=26.9035532994924, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790102, Length=201, Percent_Identity=23.3830845771144, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI87082052, Length=197, Percent_Identity=26.9035532994924, Blast_Score=66, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23961; Mature: 23961

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIKILIADDNSFIREGMKIILNTYEEFEVVDTVNDGKEAVEYCKKNDVDIALLDVRMPN
CEEEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCCCEEEEEEECCC
MNGVEATKLICEETETKPLILTTFDDDEYILDAVKNGAKGYLLKNNDPERIRDAIKGVYN
CCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCC
GQTVMQDVVLDKIKCNLMESKEDECKIDKSLFTERELSIIALIAKGFSNKEISKQLFISE
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHEECC
GTIANYITSVLGKTGLEHRTQIAIYYLTGKVD
CHHHHHHHHHHHCCCCCCCEEEEEEEEEECCC
>Mature Secondary Structure
MKIKILIADDNSFIREGMKIILNTYEEFEVVDTVNDGKEAVEYCKKNDVDIALLDVRMPN
CEEEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHCCCCCEEEEEEECCC
MNGVEATKLICEETETKPLILTTFDDDEYILDAVKNGAKGYLLKNNDPERIRDAIKGVYN
CCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCC
GQTVMQDVVLDKIKCNLMESKEDECKIDKSLFTERELSIIALIAKGFSNKEISKQLFISE
CHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHEECC
GTIANYITSVLGKTGLEHRTQIAIYYLTGKVD
CHHHHHHHHHHHCCCCCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8973323; 9384377 [H]