Click here to switch to the map view.

The map label for this gene is yfiS [H]

Identifier: 49184548

GI number: 49184548

Start: 1563383

End: 1564597

Strand: Reverse

Name: yfiS [H]

Synonym: BAS1533

Alternate gene names: 49184548

Gene position: 1564597-1563383 (Counterclockwise)

Preceding gene: 49184549

Following gene: 49184547

Centisome position: 29.92

GC content: 33.0

Gene sequence:

>1215_bases
ATGTCAGTTTCTTATTCCGCACTTCTTAAAACAAATAAAAACTTCAACAAACTATTTTACGGACAAACATTAAGTGTACT
TGGAGATTGGTTTCATACAGTAGCTTTATTAACATTCGTCTATAGCATAACAGAATCTCCGTTTATGATTGCACTTACTT
TTATAAGTAAAGGTTTACCACAACTACTTCTTAGCCCTTTCATAGGCGGAGTGGTAGATCGTTTTTCTAAAAAGAATATT
ATGATTTTTACCGATATTACACGGGGAATTCTAGTCCTTACATACATACTCGCAAGCTATAAAATTGAAATTATTTTCAT
TGCTAATATATGTTTATCTGTACTTTCTTGCTTATTTGAGCCAGCTAAACAAGCAACCTTAAAAAACATCGTACACGAAA
ATCATTTCGTAACTGCTAACTCTCTTTCTAGTACAATGAATGGTTTTATGTCTATTATGGGTGCTTCTTTAGGCGGTATA
ATTGCACAATCTTTACATATTGAGTTCGCTTTTTTAGTAAATAGCCTATCATACTTTATTTCAGCCTATTTTATTTATAG
TATGTGTATCCCTTCTCATAATACTTGTAACAAGAAAAAAGCATTTTTGACTGATATAAAAGATGGTTATACATATATAT
TGCAAACAAAAATCATTTTAACATTAATTCTTGTCGGCATTTCATGGGGTCTTATTGGCGGCGCTTATCAGCTACTTCTA
ACAATCTATGCCGAAAAAATTTTCCACACGAACATTGGAATTTTATATACGGTTCAAGGGGCTGGGCTTATGATAGGAAG
CCTTCTCGTTAATCTTTATATATCCCATAATAAAGAAAAAATAAAAAAAGCATTTGGCTGGGCCTGCTTCCTACAAGGCG
TTTTCTTTCTTGGATTCATTCTATCCAGTCAACTTATTTTCGGTCTCACTACATTACTATGTATGCGCATAGCAGGAGGA
ATCATCGTTCCACTTGATACGACATTACTTCAAACTTACACGCGTGAAAATATGATTGGTAAAGTTTTCTCCTTTCATTA
CTCTATTTACGGTTCACTTATACAGTTATCTATGCTCATGACAGGATCGTTATTAGAAATCCTTTCTCCTCAAATGATTG
GTAGTTTATTAGCTACATGCTGTATATTTGTAAGTTTTATATGGCTTTACCTGTTTTATCGCGGGAAACTTAGTGAAGAA
TCTACCATTACTTAA

Upstream 100 bases:

>100_bases
GCACAATTACCAGTTGGACTTCCTTCAGAAAAGAAAGTGTATTTAAGTGAATGGAAAAAACAAATTCAAGCATCATCATT
ACATAAGGATTGAGCTTTTT

Downstream 100 bases:

>100_bases
TAGCATGTTTATGTACATGCTATTTTTATTTATGTCGAAATACAGAGGAAAAAGGCGATTTATTTTCCTTACGTTCTATT
TTTTTAGAATGTACAATAAA

Product: permease

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSVSYSALLKTNKNFNKLFYGQTLSVLGDWFHTVALLTFVYSITESPFMIALTFISKGLPQLLLSPFIGGVVDRFSKKNI
MIFTDITRGILVLTYILASYKIEIIFIANICLSVLSCLFEPAKQATLKNIVHENHFVTANSLSSTMNGFMSIMGASLGGI
IAQSLHIEFAFLVNSLSYFISAYFIYSMCIPSHNTCNKKKAFLTDIKDGYTYILQTKIILTLILVGISWGLIGGAYQLLL
TIYAEKIFHTNIGILYTVQGAGLMIGSLLVNLYISHNKEKIKKAFGWACFLQGVFFLGFILSSQLIFGLTTLLCMRIAGG
IIVPLDTTLLQTYTRENMIGKVFSFHYSIYGSLIQLSMLMTGSLLEILSPQMIGSLLATCCIFVSFIWLYLFYRGKLSEE
STIT

Sequences:

>Translated_404_residues
MSVSYSALLKTNKNFNKLFYGQTLSVLGDWFHTVALLTFVYSITESPFMIALTFISKGLPQLLLSPFIGGVVDRFSKKNI
MIFTDITRGILVLTYILASYKIEIIFIANICLSVLSCLFEPAKQATLKNIVHENHFVTANSLSSTMNGFMSIMGASLGGI
IAQSLHIEFAFLVNSLSYFISAYFIYSMCIPSHNTCNKKKAFLTDIKDGYTYILQTKIILTLILVGISWGLIGGAYQLLL
TIYAEKIFHTNIGILYTVQGAGLMIGSLLVNLYISHNKEKIKKAFGWACFLQGVFFLGFILSSQLIFGLTTLLCMRIAGG
IIVPLDTTLLQTYTRENMIGKVFSFHYSIYGSLIQLSMLMTGSLLEILSPQMIGSLLATCCIFVSFIWLYLFYRGKLSEE
STIT
>Mature_403_residues
SVSYSALLKTNKNFNKLFYGQTLSVLGDWFHTVALLTFVYSITESPFMIALTFISKGLPQLLLSPFIGGVVDRFSKKNIM
IFTDITRGILVLTYILASYKIEIIFIANICLSVLSCLFEPAKQATLKNIVHENHFVTANSLSSTMNGFMSIMGASLGGII
AQSLHIEFAFLVNSLSYFISAYFIYSMCIPSHNTCNKKKAFLTDIKDGYTYILQTKIILTLILVGISWGLIGGAYQLLLT
IYAEKIFHTNIGILYTVQGAGLMIGSLLVNLYISHNKEKIKKAFGWACFLQGVFFLGFILSSQLIFGLTTLLCMRIAGGI
IVPLDTTLLQTYTRENMIGKVFSFHYSIYGSLIQLSMLMTGSLLEILSPQMIGSLLATCCIFVSFIWLYLFYRGKLSEES
TIT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 45014; Mature: 44883

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSYSALLKTNKNFNKLFYGQTLSVLGDWFHTVALLTFVYSITESPFMIALTFISKGLP
CCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHH
QLLLSPFIGGVVDRFSKKNIMIFTDITRGILVLTYILASYKIEIIFIANICLSVLSCLFE
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHH
PAKQATLKNIVHENHFVTANSLSSTMNGFMSIMGASLGGIIAQSLHIEFAFLVNSLSYFI
HHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAYFIYSMCIPSHNTCNKKKAFLTDIKDGYTYILQTKIILTLILVGISWGLIGGAYQLLL
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIYAEKIFHTNIGILYTVQGAGLMIGSLLVNLYISHNKEKIKKAFGWACFLQGVFFLGFI
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LSSQLIFGLTTLLCMRIAGGIIVPLDTTLLQTYTRENMIGKVFSFHYSIYGSLIQLSMLM
HHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGSLLEILSPQMIGSLLATCCIFVSFIWLYLFYRGKLSEESTIT
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SVSYSALLKTNKNFNKLFYGQTLSVLGDWFHTVALLTFVYSITESPFMIALTFISKGLP
CCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHH
QLLLSPFIGGVVDRFSKKNIMIFTDITRGILVLTYILASYKIEIIFIANICLSVLSCLFE
HHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHH
PAKQATLKNIVHENHFVTANSLSSTMNGFMSIMGASLGGIIAQSLHIEFAFLVNSLSYFI
HHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAYFIYSMCIPSHNTCNKKKAFLTDIKDGYTYILQTKIILTLILVGISWGLIGGAYQLLL
HHHHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TIYAEKIFHTNIGILYTVQGAGLMIGSLLVNLYISHNKEKIKKAFGWACFLQGVFFLGFI
HHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
LSSQLIFGLTTLLCMRIAGGIIVPLDTTLLQTYTRENMIGKVFSFHYSIYGSLIQLSMLM
HHHHHHHHHHHHHHHHHHCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TGSLLEILSPQMIGSLLATCCIFVSFIWLYLFYRGKLSEESTIT
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]