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The map label for this gene is ywcJ [H]

Identifier: 49184239

GI number: 49184239

Start: 1267131

End: 1267982

Strand: Reverse

Name: ywcJ [H]

Synonym: BAS1222

Alternate gene names: 49184239

Gene position: 1267982-1267131 (Counterclockwise)

Preceding gene: 49184240

Following gene: 49184229

Centisome position: 24.25

GC content: 35.92

Gene sequence:

>852_bases
ATGCTAGAACAAGGATTGGATTATGTTGTCAAACTGGCTAAAAGCAAAAAACAAATACTAGATGCAAACCCGATGCAATA
TTTCATTCGTGCAGCACTTGCGGGTATTTATATCGGTTTTATTATTGTTCTATGCTTTAAATTAGGTAACTTTTTTCACA
TTGCAGATTCACCAGCAACTTATTTAGCTGCTTCTATGTTTTTCGGAATTGCTCTCGTACTTATTATATATGGTGGTGCT
GAATTGTTTACTGGAAACACAATGTACTTCACTGTAGCAACATTAAGAAAAGAAACAACAATATCTGATACACTTCGTAA
CTGGGTTGCTTGTTATGCAGGTAACTTAGCGGGTGCTTTATTCTTTGCCTTACTATTTTACGCAACTGGCATTTTCGAAG
CAATCGATCACGGTCATTTAATGAATAAAGTCGTTGAGGGAAAAATGAATACACCTACAATGCAATTATTCTTTAAAGCA
ATTTTATGTAACTGGCTTGTATGTCTTGCCTGCTTCTTACCTTCCCAAGTTAAAGGGGATACAGCAAAAATAATGATCAT
GATGCTACTCGTTTTCACATTTTTCTTATCTGGTTACGAGCATAGCATCGCAAACTTATCACTGTTTGCTCTATCTTTAG
TTTCACCGCATCCTGAGACAATTTCTTTTGCAGGTGCTATTCATAACTTAATTCCAGTTACAATCGGTAACATTATTGGT
GGGTCAGTATTTGTCGGTATGGTATACCACTACTTAACAAAGAAAGTACCTGACGTGAAACAAGAAGACACTGAATTAGT
AGCAGCTCAACAAGAAGAAATTATTCCTCTGCAAGCGCATATGAAACATTAA

Upstream 100 bases:

>100_bases
GTGAAACATTGCACAAACTTATTGAAACTATGACAGAATCCATGTACTTTATTCATATAAGGAGTATTTATAGATGATGT
ACTAAAGGAGCGTATTACTC

Downstream 100 bases:

>100_bases
AACTCCAAAGTTTTACTTTGGAGTTTTTAGCTCCCAACATATTTAACAACAAGATTCGGATTAATCTCTCCTTCTTCCTT
CCCCTTATTAATTCTCTGTT

Product: formate/nitrite transporter family protein

Products: Proton [Cytoplasm]; nitrite [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MLEQGLDYVVKLAKSKKQILDANPMQYFIRAALAGIYIGFIIVLCFKLGNFFHIADSPATYLAASMFFGIALVLIIYGGA
ELFTGNTMYFTVATLRKETTISDTLRNWVACYAGNLAGALFFALLFYATGIFEAIDHGHLMNKVVEGKMNTPTMQLFFKA
ILCNWLVCLACFLPSQVKGDTAKIMIMMLLVFTFFLSGYEHSIANLSLFALSLVSPHPETISFAGAIHNLIPVTIGNIIG
GSVFVGMVYHYLTKKVPDVKQEDTELVAAQQEEIIPLQAHMKH

Sequences:

>Translated_283_residues
MLEQGLDYVVKLAKSKKQILDANPMQYFIRAALAGIYIGFIIVLCFKLGNFFHIADSPATYLAASMFFGIALVLIIYGGA
ELFTGNTMYFTVATLRKETTISDTLRNWVACYAGNLAGALFFALLFYATGIFEAIDHGHLMNKVVEGKMNTPTMQLFFKA
ILCNWLVCLACFLPSQVKGDTAKIMIMMLLVFTFFLSGYEHSIANLSLFALSLVSPHPETISFAGAIHNLIPVTIGNIIG
GSVFVGMVYHYLTKKVPDVKQEDTELVAAQQEEIIPLQAHMKH
>Mature_283_residues
MLEQGLDYVVKLAKSKKQILDANPMQYFIRAALAGIYIGFIIVLCFKLGNFFHIADSPATYLAASMFFGIALVLIIYGGA
ELFTGNTMYFTVATLRKETTISDTLRNWVACYAGNLAGALFFALLFYATGIFEAIDHGHLMNKVVEGKMNTPTMQLFFKA
ILCNWLVCLACFLPSQVKGDTAKIMIMMLLVFTFFLSGYEHSIANLSLFALSLVSPHPETISFAGAIHNLIPVTIGNIIG
GSVFVGMVYHYLTKKVPDVKQEDTELVAAQQEEIIPLQAHMKH

Specific function: Potential Nitrite Transporter. [C]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI87082249, Length=274, Percent_Identity=40.1459854014599, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1787132, Length=263, Percent_Identity=31.1787072243346, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI1788837, Length=278, Percent_Identity=30.5755395683453, Blast_Score=89, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321779, Length=190, Percent_Identity=32.6315789473684, Blast_Score=96, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 31288; Mature: 31288

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS01005 FORMATE_NITRITE_TP_1 ; PS01006 FORMATE_NITRITE_TP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; nitrite [Periplasm] [C]

Specific reaction: Proton [Periplasm] + nitrite [Periplasm] = Proton [Cytoplasm] + nitrite [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2163394; 7934828; 9384377 [H]