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The map label for this gene is cls

Identifier: 49183617

GI number: 49183617

Start: 639802

End: 641331

Strand: Reverse

Name: cls

Synonym: BAS0592

Alternate gene names: 49183617

Gene position: 641331-639802 (Counterclockwise)

Preceding gene: 49183621

Following gene: 49183616

Centisome position: 12.27

GC content: 35.49

Gene sequence:

>1530_bases
ATGAAGAAACCTATCATCCAATTACTATTAATATTTACAATTGTATCTATCGTTCCTTTCCTATTAAATACATCTTATAT
TTCGTTATATACATTTGTCGGCGTTCTCTGGTCCATCACAATTGTAGGAATTTCATTCGTTATTTTTATCGAAAATAGAT
CTCCTCAAAGTACCTTAGCCTGGTTTTTAGTATTAGCACTTCTTCCCGTTGTAGGTGTGCTTTTATACTCTATTTTCGGG
CGTAGCCGTTGGAGAAGAAAAAAACATCTCCATCGTTCAGAAGAGCAAAGAAAGTTATTCCGTGAAATATTAGAAGGAAG
ACGACTAGAGTTATCACTCAAAGTCCCATTAAGTGAACGCTCTGTCCATTTAACAGAAGTCGTACAAAAATTTGGAGGCG
GGCCTGCAGCAGATAGAACGACTACCAAACTTTTAACAAACGGAGATCAAACATTCTCAGAAATTTTGCAAGCGATCGAG
CAAGCAAAACATCACATACATATTCAATACTATATTTATAAATCTGATGAGATTGGTACAAAAGTTCGGGATGCGTTAAT
CAAAAAGGCAAAAGATGGTGTTATTGTACGCTTCCTTTATGATGGACTCGGAAGTAATACGTTGAGAAGACGGTTTTTAC
AACCTATGAAAGAAGCTGGAATTGAAATTGTTGAATTTGATCCTATTTTTTCAGCTTGGTTACTTGAAACCGTCAATTAT
CGTAACCACCGTAAAATCGTCATTGTAGATGGAGAAATCGGTTTTACTGGTGGACTCAACGTCGGTGATGAATATCTTGG
TCGTTCAAAAAAATTTCCCGTTTGGCGTGACAGTCATTTAAAAGTAGAAGGAAAAGCGCTATATAAATTACAAGCAATCT
TTCTAGAGGATTGGCTCTATGCATCTAGTGGATTGAATACTTATTCTTGGGATCCATTTATGAATAGACAATACTTCCCT
GGTAAAGAAATTTCAAACGCAGAAGGTGCTGTGCAAATTGTAGCGAGCGGACCAAGCTCAGATGATAAAAGCATTCGCAA
TACATTATTAGCTGTTATGGGCTCGGCAAAAAAATCTATTTGGATTGCTACACCCTACTTTATTCCAGATCAAGAAACTT
TAACATTATTACGTTTAAGTGCAATATCTGGCATAGATGTACGCATTTTATATCCTGGTAAAAGCGATAGTATTATTAGT
GACCAAGCATCTCAGTCCTACTTCACTCCACTTTTAAAAGCTGGTGCCTCCATTTATAGTTATAAAGATGGCTTTATGCA
TGCAAAAATTTTACTTGTTGATGATAAAATCGCAACAATCGGAACAGCAAATATGGACGTACGCAGCTTTGAATTGAATT
ACGAAATTATTAGCGTACTGTATGAATCAGAAACTGTTCATGATATTAAACGTGATTTTGAAGATGACTTTAAGCATTCT
ACTGAAATTAAATGGAACGCTTTCCAAAAGAGAAGTATCAAAAAACGAATATTAGAATCTTTTATGAGGCTCATTTCTCC
TTTACTATAA

Upstream 100 bases:

>100_bases
ATTTTGTAAAAAATAGAAAACACTTTATAATAAAAATGTCACAGTACATCTGCTATTTAGGAAATCTATATTGAGGAAAG
ATTAGAGGAATTATATAGAA

Downstream 100 bases:

>100_bases
GCAATCAATGTATTATTGATTGCTTTTTCTTTTATATTTGTCCCCTCTCCTTTTCTTTGCATACCATATTAAAACAAGTT
ATCATAAGATGTATTACTTT

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase 1

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MKKPIIQLLLIFTIVSIVPFLLNTSYISLYTFVGVLWSITIVGISFVIFIENRSPQSTLAWFLVLALLPVVGVLLYSIFG
RSRWRRKKHLHRSEEQRKLFREILEGRRLELSLKVPLSERSVHLTEVVQKFGGGPAADRTTTKLLTNGDQTFSEILQAIE
QAKHHIHIQYYIYKSDEIGTKVRDALIKKAKDGVIVRFLYDGLGSNTLRRRFLQPMKEAGIEIVEFDPIFSAWLLETVNY
RNHRKIVIVDGEIGFTGGLNVGDEYLGRSKKFPVWRDSHLKVEGKALYKLQAIFLEDWLYASSGLNTYSWDPFMNRQYFP
GKEISNAEGAVQIVASGPSSDDKSIRNTLLAVMGSAKKSIWIATPYFIPDQETLTLLRLSAISGIDVRILYPGKSDSIIS
DQASQSYFTPLLKAGASIYSYKDGFMHAKILLVDDKIATIGTANMDVRSFELNYEIISVLYESETVHDIKRDFEDDFKHS
TEIKWNAFQKRSIKKRILESFMRLISPLL

Sequences:

>Translated_509_residues
MKKPIIQLLLIFTIVSIVPFLLNTSYISLYTFVGVLWSITIVGISFVIFIENRSPQSTLAWFLVLALLPVVGVLLYSIFG
RSRWRRKKHLHRSEEQRKLFREILEGRRLELSLKVPLSERSVHLTEVVQKFGGGPAADRTTTKLLTNGDQTFSEILQAIE
QAKHHIHIQYYIYKSDEIGTKVRDALIKKAKDGVIVRFLYDGLGSNTLRRRFLQPMKEAGIEIVEFDPIFSAWLLETVNY
RNHRKIVIVDGEIGFTGGLNVGDEYLGRSKKFPVWRDSHLKVEGKALYKLQAIFLEDWLYASSGLNTYSWDPFMNRQYFP
GKEISNAEGAVQIVASGPSSDDKSIRNTLLAVMGSAKKSIWIATPYFIPDQETLTLLRLSAISGIDVRILYPGKSDSIIS
DQASQSYFTPLLKAGASIYSYKDGFMHAKILLVDDKIATIGTANMDVRSFELNYEIISVLYESETVHDIKRDFEDDFKHS
TEIKWNAFQKRSIKKRILESFMRLISPLL
>Mature_509_residues
MKKPIIQLLLIFTIVSIVPFLLNTSYISLYTFVGVLWSITIVGISFVIFIENRSPQSTLAWFLVLALLPVVGVLLYSIFG
RSRWRRKKHLHRSEEQRKLFREILEGRRLELSLKVPLSERSVHLTEVVQKFGGGPAADRTTTKLLTNGDQTFSEILQAIE
QAKHHIHIQYYIYKSDEIGTKVRDALIKKAKDGVIVRFLYDGLGSNTLRRRFLQPMKEAGIEIVEFDPIFSAWLLETVNY
RNHRKIVIVDGEIGFTGGLNVGDEYLGRSKKFPVWRDSHLKVEGKALYKLQAIFLEDWLYASSGLNTYSWDPFMNRQYFP
GKEISNAEGAVQIVASGPSSDDKSIRNTLLAVMGSAKKSIWIATPYFIPDQETLTLLRLSAISGIDVRILYPGKSDSIIS
DQASQSYFTPLLKAGASIYSYKDGFMHAKILLVDDKIATIGTANMDVRSFELNYEIISVLYESETVHDIKRDFEDDFKHS
TEIKWNAFQKRSIKKRILESFMRLISPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains

Homologues:

Organism=Escherichia coli, GI1787502, Length=497, Percent_Identity=27.5653923541247, Blast_Score=205, Evalue=7e-54,
Organism=Escherichia coli, GI1787007, Length=330, Percent_Identity=27.2727272727273, Blast_Score=131, Evalue=1e-31,
Organism=Escherichia coli, GI87081826, Length=381, Percent_Identity=26.7716535433071, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CLS1_BACAN (Q81V75)

Other databases:

- EMBL:   AE016879
- EMBL:   AE017334
- EMBL:   AE017225
- RefSeq:   NP_843156.1
- RefSeq:   YP_017253.1
- RefSeq:   YP_026869.1
- ProteinModelPortal:   Q81V75
- EnsemblBacteria:   EBBACT00000012327
- EnsemblBacteria:   EBBACT00000014637
- EnsemblBacteria:   EBBACT00000023712
- GeneID:   1088023
- GeneID:   2814428
- GeneID:   2850776
- GenomeReviews:   AE016879_GR
- GenomeReviews:   AE017225_GR
- GenomeReviews:   AE017334_GR
- KEGG:   ban:BA_0625
- KEGG:   bar:GBAA_0625
- KEGG:   bat:BAS0592
- TIGR:   BA_0625
- TIGR:   GBAA_0625
- GeneTree:   EBGT00070000032016
- HOGENOM:   HBG694312
- OMA:   HLKVEGK
- ProtClustDB:   PRK12452
- BioCyc:   BANT260799:BAS0592-MONOMER
- BioCyc:   BANT261594:GBAA0625-MONOMER
- HAMAP:   MF_00190
- InterPro:   IPR022924
- InterPro:   IPR001736
- SMART:   SM00155

Pfam domain/function: PF00614 PLDc

EC number: 2.7.8.- [C]

Molecular weight: Translated: 58108; Mature: 58108

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: PS50035 PLD

Important sites: ACT_SITE 243-243 ACT_SITE 245-245 ACT_SITE 250-250 ACT_SITE 427-427 ACT_SITE 429-429 ACT_SITE 434-434

Signals:

None

Transmembrane regions:

HASH(0xdc95310)-; HASH(0xe407f48)-; HASH(0xf068d9c)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKPIIQLLLIFTIVSIVPFLLNTSYISLYTFVGVLWSITIVGISFVIFIENRSPQSTLA
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHH
WFLVLALLPVVGVLLYSIFGRSRWRRKKHLHRSEEQRKLFREILEGRRLELSLKVPLSER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCC
SVHLTEVVQKFGGGPAADRTTTKLLTNGDQTFSEILQAIEQAKHHIHIQYYIYKSDEIGT
CCHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCH
KVRDALIKKAKDGVIVRFLYDGLGSNTLRRRFLQPMKEAGIEIVEFDPIFSAWLLETVNY
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCC
RNHRKIVIVDGEIGFTGGLNVGDEYLGRSKKFPVWRDSHLKVEGKALYKLQAIFLEDWLY
CCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHH
ASSGLNTYSWDPFMNRQYFPGKEISNAEGAVQIVASGPSSDDKSIRNTLLAVMGSAKKSI
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEE
WIATPYFIPDQETLTLLRLSAISGIDVRILYPGKSDSIISDQASQSYFTPLLKAGASIYS
EEECCEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHEE
YKDGFMHAKILLVDDKIATIGTANMDVRSFELNYEIISVLYESETVHDIKRDFEDDFKHS
ECCCCEEEEEEEEECCEEEEECCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHCCC
TEIKWNAFQKRSIKKRILESFMRLISPLL
CCCEEHHHHHHHHHHHHHHHHHHHHHCCH
>Mature Secondary Structure
MKKPIIQLLLIFTIVSIVPFLLNTSYISLYTFVGVLWSITIVGISFVIFIENRSPQSTLA
CCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCHHHHH
WFLVLALLPVVGVLLYSIFGRSRWRRKKHLHRSEEQRKLFREILEGRRLELSLKVPLSER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCCCC
SVHLTEVVQKFGGGPAADRTTTKLLTNGDQTFSEILQAIEQAKHHIHIQYYIYKSDEIGT
CCHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCH
KVRDALIKKAKDGVIVRFLYDGLGSNTLRRRFLQPMKEAGIEIVEFDPIFSAWLLETVNY
HHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHHCC
RNHRKIVIVDGEIGFTGGLNVGDEYLGRSKKFPVWRDSHLKVEGKALYKLQAIFLEDWLY
CCCCEEEEEECCCCCCCCCCCCHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHH
ASSGLNTYSWDPFMNRQYFPGKEISNAEGAVQIVASGPSSDDKSIRNTLLAVMGSAKKSI
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCEE
WIATPYFIPDQETLTLLRLSAISGIDVRILYPGKSDSIISDQASQSYFTPLLKAGASIYS
EEECCEECCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHEE
YKDGFMHAKILLVDDKIATIGTANMDVRSFELNYEIISVLYESETVHDIKRDFEDDFKHS
ECCCCEEEEEEEEECCEEEEECCCCEEEEEECCHHHHHHHHCCHHHHHHHHHHHHHHCCC
TEIKWNAFQKRSIKKRILESFMRLISPLL
CCCEEHHHHHHHHHHHHHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12721629