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The map label for this gene is mrcA [H]

Identifier: 49183538

GI number: 49183538

Start: 541382

End: 542158

Strand: Direct

Name: mrcA [H]

Synonym: BAS0512

Alternate gene names: 49183538

Gene position: 541382-542158 (Clockwise)

Preceding gene: 49183537

Following gene: 49183539

Centisome position: 10.35

GC content: 33.72

Gene sequence:

>777_bases
ATGAAAGAACGAGTATTATCTAGGGTGAACTATCATCAAAAAGTTGCATTAAATCCAATGACTAAGTTTTTTTATAAAGC
CATTATTTTATTATTATTGTTAAGTTTTACGTTATTATTCATTGGAAATGTAATGATCGAGCAAAGTGATATTAGTAAAT
TACATGTACCGGCTAAGTTAGAGGTGCCAGAATCATTAACACATGCATTTATTGCGACAGAAGATAAAAGGTTTTATCAT
CATAACGGTTTAGATTATATAGCGATTATTAGGGCGTCTGTTGAGAATATAAAAGCTGGTGGTGTTGTGCAAGGCGGTAG
CACAATTACACAACAGTTATCAAAAAATGCTTTTTTAACGAATGAACGTACATTTTCTCGTAAGTGGAAAGAAATTTTTT
ATACGAAAAAAATTGAACGTACATTTACAAAGGATGAAATTTTAAAATTATATGTAAGTAATATTTATTACGGAGAAGGG
GCATGGGGAATTGAAAAAGCAGCAAACCTTTACTTCGGTAAAAAGGTGAGCCAATTAACGTTGGCTGAAAGTGCAATGAT
GGCTGCTGTAGTAAAGGCACCTGCTTATTACTCACCTGCACAAAATTACGATAAAGCAGTTGAGAGACGAAACGTCGTTT
TGAGATTAATGGAGAAAGAAGGCTATATCAATCATGATGAGTATATGCAAGCAGTTAGTGAGAAATTAGTCATTCGTCAT
GATATAAAAACAGAGCGGTCGATGTTAAATCATGCGCGCGAAAAAGCAGTGAGCTAA

Upstream 100 bases:

>100_bases
TGATGTAAGGTACGTGTAAGAGAAATCGATGGGATATTTTTTTACAACAAGGTATTCTTAAAGTAAGAGTTATACGCTAT
GTGGAAAAGAGGTGTTTAAG

Downstream 100 bases:

>100_bases
GGGAAGTTCCTACATAGGAGCTTCTTTTTTTGAATAAATTGTGACAAATTGTGACACAATATCGTCACAATTGTTGTATT
TTTGTAATTTATGTGAGCGT

Product: penicillin-binding domain-containing protein

Products: NA

Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MKERVLSRVNYHQKVALNPMTKFFYKAIILLLLLSFTLLFIGNVMIEQSDISKLHVPAKLEVPESLTHAFIATEDKRFYH
HNGLDYIAIIRASVENIKAGGVVQGGSTITQQLSKNAFLTNERTFSRKWKEIFYTKKIERTFTKDEILKLYVSNIYYGEG
AWGIEKAANLYFGKKVSQLTLAESAMMAAVVKAPAYYSPAQNYDKAVERRNVVLRLMEKEGYINHDEYMQAVSEKLVIRH
DIKTERSMLNHAREKAVS

Sequences:

>Translated_258_residues
MKERVLSRVNYHQKVALNPMTKFFYKAIILLLLLSFTLLFIGNVMIEQSDISKLHVPAKLEVPESLTHAFIATEDKRFYH
HNGLDYIAIIRASVENIKAGGVVQGGSTITQQLSKNAFLTNERTFSRKWKEIFYTKKIERTFTKDEILKLYVSNIYYGEG
AWGIEKAANLYFGKKVSQLTLAESAMMAAVVKAPAYYSPAQNYDKAVERRNVVLRLMEKEGYINHDEYMQAVSEKLVIRH
DIKTERSMLNHAREKAVS
>Mature_258_residues
MKERVLSRVNYHQKVALNPMTKFFYKAIILLLLLSFTLLFIGNVMIEQSDISKLHVPAKLEVPESLTHAFIATEDKRFYH
HNGLDYIAIIRASVENIKAGGVVQGGSTITQQLSKNAFLTNERTFSRKWKEIFYTKKIERTFTKDEILKLYVSNIYYGEG
AWGIEKAANLYFGKKVSQLTLAESAMMAAVVKAPAYYSPAQNYDKAVERRNVVLRLMEKEGYINHDEYMQAVSEKLVIRH
DIKTERSMLNHAREKAVS

Specific function: Cell Wall Formation. Synthesis Of Cross-Linked Peptidoglycan From The Lipid Intermediates. The Enzyme Has A Penicillin-Insensitive Transglycosylase N-Terminal Domain (Formation Of Linear Glycan Strands) And A Penicillin-Sensitive Transpeptidase C-Terminal

COG id: COG5009

COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI87082258, Length=185, Percent_Identity=48.6486486486487, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1786343, Length=163, Percent_Identity=39.2638036809816, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1789601, Length=162, Percent_Identity=35.8024691358025, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI1788867, Length=171, Percent_Identity=29.2397660818713, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.2.-; 3.4.-.- [C]

Molecular weight: Translated: 29649; Mature: 29649

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKERVLSRVNYHQKVALNPMTKFFYKAIILLLLLSFTLLFIGNVMIEQSDISKLHVPAKL
CCHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCC
EVPESLTHAFIATEDKRFYHHNGLDYIAIIRASVENIKAGGVVQGGSTITQQLSKNAFLT
CCCHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCCCCC
NERTFSRKWKEIFYTKKIERTFTKDEILKLYVSNIYYGEGAWGIEKAANLYFGKKVSQLT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCHHHHHH
LAESAMMAAVVKAPAYYSPAQNYDKAVERRNVVLRLMEKEGYINHDEYMQAVSEKLVIRH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
DIKTERSMLNHAREKAVS
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKERVLSRVNYHQKVALNPMTKFFYKAIILLLLLSFTLLFIGNVMIEQSDISKLHVPAKL
CCHHHHHHHCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCC
EVPESLTHAFIATEDKRFYHHNGLDYIAIIRASVENIKAGGVVQGGSTITQQLSKNAFLT
CCCHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCCCCC
NERTFSRKWKEIFYTKKIERTFTKDEILKLYVSNIYYGEGAWGIEKAANLYFGKKVSQLT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCHHHHHH
LAESAMMAAVVKAPAYYSPAQNYDKAVERRNVVLRLMEKEGYINHDEYMQAVSEKLVIRH
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
DIKTERSMLNHAREKAVS
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]