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The map label for this gene is purD [H]

Identifier: 49183314

GI number: 49183314

Start: 307243

End: 308514

Strand: Direct

Name: purD [H]

Synonym: BAS0286

Alternate gene names: 49183314

Gene position: 307243-308514 (Clockwise)

Preceding gene: 49183313

Following gene: 49183318

Centisome position: 5.88

GC content: 38.05

Gene sequence:

>1272_bases
ATGAATGTTTTAGTAATTGGCCGCGGTGGGCGTGAGCATGCTTTAGCTTGGAAGTTTGCACAATCTGAAAAAGTAGAAAA
GGTATATGTAGCACCAGGTAATGAAGGTATGCGTGATGTTGCAACACCAATTGATATTGATGAAAATGATTTTGATGCAT
TAGTTTTATTTGCGAAAGAGAACCATGTGGAATTAACTTTCGTTGGACCAGAAATTCCACTTATGAATGGAATTGTTGAT
CGTTTTAAAGAAGAGGGACTTCGCGTATTTGGTCCGAATAAAGCAGCTGCTGTTATTGAAGGTAGTAAAGCTTTTACAAA
AGAGCTTATGAAAAAATATAATATTCCAACTGCAGCGTACGAAACTTTTACAGATTATGAAGAAGCAGTACAGTACATTG
AAAAAGTTGGTGCACCAATTGTTATTAAAGCGGATGGTCTAGCTGCTGGTAAAGGTGTAACAGTAGCGATGACGCTTGAA
GAGGCATTACAAGCTGCGAAAGAAATGCTGCAAGATGTAAAGTTCGGGGAAGCGAGTAAGAAAGTTGTTATTGAAGAGTT
TTTAGATGGACAAGAATTTTCATTAATGGCATTTGTGAATGGAACAACTGTACATCCGATGGTAATTGCGCAAGATCATA
AACGAGCTTTTGATGGTGATAAAGGTCCAAATACTGGCGGAATGGGTGCATATTCTCCAGTACCACAAATTTCGGAGTCA
GCAGTTCAAGAGGCGATTGAAACGGTGTTATATCCAACTGCTAAAGCAATGATCCAAGAAAATCGATCGTTTACAGGAAT
TTTATATGCGGGACTTATTTTAACAAATGATGGTCCAAAGGTAATTGAATTTAATGCACGTTTTGGCGATCCTGAAACTG
AAGTTGTATTACCCCGTTTAGAAAATGATTTAGTGGATGTATGTAACGCTGTATTAGATGAAAGTGAGTTAACGTTACAA
TGGTCAGATGAAGCCGTAATTGGTGTTGTACTTGCTGCGAAAGGATATCCGGAAGCATATAAAAAAGGTGACATTATTAA
AGGGCTAGATGCATTGCAAGATGTGATTGTTTTCCATGCAGGTACAGCAATGAAACGTGGTGACTTTGTAACGAACGGTG
GCCGTGTATTATTTGTTGCTTGTAAGGCGAATAGTTTACAAGAAGCGAAAGATAAGGTGTATAAAGAAATCGGTAAAATT
GAGAGTGATGTTCTATTTTACCGAAGTGATATAGGATATCGTGCAATTGGGCATGAGATGACGAGAAGTTAA

Upstream 100 bases:

>100_bases
GGTTAAGATAACCTTCCTTTTTAAACTTTGATTGATATGAAAAAACAAACTAGGAGCATAGCTTCTAGTTTGTACTTATT
TAAATATTGGGGGATGAAAT

Downstream 100 bases:

>100_bases
TATGTAAAAATCCCGCTGATTCCTCAGCGGGATTTTTATGCATCTTTATCTTTTTTTAATTTACCTTTTTGCTTTGCCAT
TTCTCGAAGTAAATACTCGA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPIDIDENDFDALVLFAKENHVELTFVGPEIPLMNGIVD
RFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYNIPTAAYETFTDYEEAVQYIEKVGAPIVIKADGLAAGKGVTVAMTLE
EALQAAKEMLQDVKFGEASKKVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISES
AVQEAIETVLYPTAKAMIQENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRLENDLVDVCNAVLDESELTLQ
WSDEAVIGVVLAAKGYPEAYKKGDIIKGLDALQDVIVFHAGTAMKRGDFVTNGGRVLFVACKANSLQEAKDKVYKEIGKI
ESDVLFYRSDIGYRAIGHEMTRS

Sequences:

>Translated_423_residues
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPIDIDENDFDALVLFAKENHVELTFVGPEIPLMNGIVD
RFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYNIPTAAYETFTDYEEAVQYIEKVGAPIVIKADGLAAGKGVTVAMTLE
EALQAAKEMLQDVKFGEASKKVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISES
AVQEAIETVLYPTAKAMIQENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRLENDLVDVCNAVLDESELTLQ
WSDEAVIGVVLAAKGYPEAYKKGDIIKGLDALQDVIVFHAGTAMKRGDFVTNGGRVLFVACKANSLQEAKDKVYKEIGKI
ESDVLFYRSDIGYRAIGHEMTRS
>Mature_423_residues
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPIDIDENDFDALVLFAKENHVELTFVGPEIPLMNGIVD
RFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYNIPTAAYETFTDYEEAVQYIEKVGAPIVIKADGLAAGKGVTVAMTLE
EALQAAKEMLQDVKFGEASKKVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISES
AVQEAIETVLYPTAKAMIQENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRLENDLVDVCNAVLDESELTLQ
WSDEAVIGVVLAAKGYPEAYKKGDIIKGLDALQDVIVFHAGTAMKRGDFVTNGGRVLFVACKANSLQEAKDKVYKEIGKI
ESDVLFYRSDIGYRAIGHEMTRS

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=423, Percent_Identity=48.2269503546099, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI4503915, Length=423, Percent_Identity=48.2269503546099, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI209869995, Length=423, Percent_Identity=48.2269503546099, Blast_Score=385, Evalue=1e-107,
Organism=Homo sapiens, GI209869993, Length=423, Percent_Identity=48.2269503546099, Blast_Score=385, Evalue=1e-107,
Organism=Escherichia coli, GI1790438, Length=428, Percent_Identity=51.6355140186916, Blast_Score=425, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17567511, Length=422, Percent_Identity=40.9952606635071, Blast_Score=305, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6321203, Length=439, Percent_Identity=43.2801822323462, Blast_Score=330, Evalue=4e-91,
Organism=Drosophila melanogaster, GI62471619, Length=430, Percent_Identity=46.9767441860465, Blast_Score=355, Evalue=3e-98,
Organism=Drosophila melanogaster, GI24582400, Length=430, Percent_Identity=46.9767441860465, Blast_Score=354, Evalue=5e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46215; Mature: 46215

Theoretical pI: Translated: 4.59; Mature: 4.59

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPIDIDENDFDALVLFAKE
CEEEEEECCCCCCCEEEEECCCCCCCEEEECCCCCCHHHHCCCCCCCCCCCCEEEEEEEC
NHVELTFVGPEIPLMNGIVDRFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYNIPTAAY
CCEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHCCCHHHH
ETFTDYEEAVQYIEKVGAPIVIKADGLAAGKGVTVAMTLEEALQAAKEMLQDVKFGEASK
HHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHH
KVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISES
HHHHHHHCCCCCEEEEEEECCCEECEEEEECCCHHHCCCCCCCCCCCCCCCCCCCHHHHH
AVQEAIETVLYPTAKAMIQENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCEEECCHH
ENDLVDVCNAVLDESELTLQWSDEAVIGVVLAAKGYPEAYKKGDIIKGLDALQDVIVFHA
HHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHC
GTAMKRGDFVTNGGRVLFVACKANSLQEAKDKVYKEIGKIESDVLFYRSDIGYRAIGHEM
CCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCHHH
TRS
HCC
>Mature Secondary Structure
MNVLVIGRGGREHALAWKFAQSEKVEKVYVAPGNEGMRDVATPIDIDENDFDALVLFAKE
CEEEEEECCCCCCCEEEEECCCCCCCEEEECCCCCCHHHHCCCCCCCCCCCCEEEEEEEC
NHVELTFVGPEIPLMNGIVDRFKEEGLRVFGPNKAAAVIEGSKAFTKELMKKYNIPTAAY
CCEEEEEECCCCCHHHHHHHHHHHCCCEEECCCCCEEEEECCHHHHHHHHHHHCCCHHHH
ETFTDYEEAVQYIEKVGAPIVIKADGLAAGKGVTVAMTLEEALQAAKEMLQDVKFGEASK
HHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCHH
KVVIEEFLDGQEFSLMAFVNGTTVHPMVIAQDHKRAFDGDKGPNTGGMGAYSPVPQISES
HHHHHHHCCCCCEEEEEEECCCEECEEEEECCCHHHCCCCCCCCCCCCCCCCCCCHHHHH
AVQEAIETVLYPTAKAMIQENRSFTGILYAGLILTNDGPKVIEFNARFGDPETEVVLPRL
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCEEEEEECCCCCCCCEEECCHH
ENDLVDVCNAVLDESELTLQWSDEAVIGVVLAAKGYPEAYKKGDIIKGLDALQDVIVFHA
HHHHHHHHHHHHCCCCEEEEECCCCEEEEEEECCCCCHHHHCCCHHHHHHHHHHHHHHHC
GTAMKRGDFVTNGGRVLFVACKANSLQEAKDKVYKEIGKIESDVLFYRSDIGYRAIGHEM
CCCCCCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCHHH
TRS
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]