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The map label for this gene is cysK [H]

Identifier: 49183102

GI number: 49183102

Start: 73505

End: 74428

Strand: Direct

Name: cysK [H]

Synonym: BAS0067

Alternate gene names: 49183102

Gene position: 73505-74428 (Clockwise)

Preceding gene: 49183101

Following gene: 49183103

Centisome position: 1.41

GC content: 39.94

Gene sequence:

>924_bases
ATGCGAGTGGCACAATCAGTTTCAGAATTAATCGGGAAAACGCCGATCGTTAAGTTGAACCGCATCGTAGAATCAGACAG
CGCAGATATATACTTAAAATTAGAATTTATGAATCCGGGGAGCAGCGTTAAAGATCGTATCGCATTAGCTATGATTGAAG
ATGCTGAGAAAAAAGGATTATTAAAAGAAGGCGATACAATCATTGAGCCGACAAGTGGTAACACAGGAATTGGCCTAGCG
ATGGTAGCAGCTGCTAAAGGATATAAGGCAATTTTAGTAATGCCAGAAACAATGAGCGTTGAGCGTCGCAATTTATTACG
TGCTTATGGCGCTGAATTAGTATTGACTCCAGGGCCTGAAGGAATGGGCGGAGCAATTCGTCAAGCGACTGAATTAGCAA
AAGAACATGGCTACTTTATACCGCAACAGTTCAAAAATCAATCAAATCCAGAAATTCATCGTTTAACAACAGGTCCAGAA
ATTGTTGAGCAAATGGGTGATCAATTAGATGCATTTATTGCAGGTATTGGTACAGGTGGAACGATTACTGGTGCTGGTGA
AGTGCTGAAGGAAGCATATAAAGATATTAAAATTTATGCAGTAGAACCTGCGGATTCACCGGTGTTATCTGGTGGAAAAC
CGGGTCCACATAAAATCCAAGGAATTGGGGCTGGATTTGTTCCAGAAACATTGGATGTAGAAGTATATGATGAAATCATT
CAAGTGAAAACAGAGCAAGCGTTCGAATACGCAAGAAGAGTGGCTAAAGAGGAAGGTATTTTAGTTGGTATCTCTTCAGG
AGCGGTTATTTATGCAGCGACAGAAGTTGCGAAAAAGTTAGGTAAAGGGAAAAAGGTACTTGTTATTATCCCAAGTAATG
GTGAACGTTATTTAAGTACACCACTTTATCAATTTGAGTCTTAA

Upstream 100 bases:

>100_bases
GGAATTTACCCTCTAAATACATTGACAAACAGGGAATTTTCTGACAAAATCTAAATATAGACAAAACCAATAAAAATACT
CGGTGTTAGGAGTGACAGGA

Downstream 100 bases:

>100_bases
TTACATGTGATTGAAAAAGCATCCTTGAAAAAGGATGCTTTTTCTTTTTGGATTGGAAATAGGAAAAGAGTGAATGACTA
GAAAACTTCATGTAAAATAA

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase; O-acetylserine (thiol)-lyase; OAS-TL; Superoxide-inducible protein 11; SOI11 [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRVAQSVSELIGKTPIVKLNRIVESDSADIYLKLEFMNPGSSVKDRIALAMIEDAEKKGLLKEGDTIIEPTSGNTGIGLA
MVAAAKGYKAILVMPETMSVERRNLLRAYGAELVLTPGPEGMGGAIRQATELAKEHGYFIPQQFKNQSNPEIHRLTTGPE
IVEQMGDQLDAFIAGIGTGGTITGAGEVLKEAYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVEVYDEII
QVKTEQAFEYARRVAKEEGILVGISSGAVIYAATEVAKKLGKGKKVLVIIPSNGERYLSTPLYQFES

Sequences:

>Translated_307_residues
MRVAQSVSELIGKTPIVKLNRIVESDSADIYLKLEFMNPGSSVKDRIALAMIEDAEKKGLLKEGDTIIEPTSGNTGIGLA
MVAAAKGYKAILVMPETMSVERRNLLRAYGAELVLTPGPEGMGGAIRQATELAKEHGYFIPQQFKNQSNPEIHRLTTGPE
IVEQMGDQLDAFIAGIGTGGTITGAGEVLKEAYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVEVYDEII
QVKTEQAFEYARRVAKEEGILVGISSGAVIYAATEVAKKLGKGKKVLVIIPSNGERYLSTPLYQFES
>Mature_307_residues
MRVAQSVSELIGKTPIVKLNRIVESDSADIYLKLEFMNPGSSVKDRIALAMIEDAEKKGLLKEGDTIIEPTSGNTGIGLA
MVAAAKGYKAILVMPETMSVERRNLLRAYGAELVLTPGPEGMGGAIRQATELAKEHGYFIPQQFKNQSNPEIHRLTTGPE
IVEQMGDQLDAFIAGIGTGGTITGAGEVLKEAYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVEVYDEII
QVKTEQAFEYARRVAKEEGILVGISSGAVIYAATEVAKKLGKGKKVLVIIPSNGERYLSTPLYQFES

Specific function: Catalyzes the conversion of O-acetylserine to cysteine. Also acts as a sensor of cysteine availability in the signal transduction pathway modulating CymR activity. When cysteine is present, the pool of O-acetylserine (OAS) is low, which leads to the forma

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=303, Percent_Identity=43.5643564356436, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI295821200, Length=303, Percent_Identity=43.5643564356436, Blast_Score=238, Evalue=4e-63,
Organism=Homo sapiens, GI4557415, Length=303, Percent_Identity=43.5643564356436, Blast_Score=238, Evalue=4e-63,
Organism=Escherichia coli, GI1788754, Length=313, Percent_Identity=52.7156549520767, Blast_Score=281, Evalue=5e-77,
Organism=Escherichia coli, GI2367138, Length=298, Percent_Identity=41.6107382550336, Blast_Score=228, Evalue=4e-61,
Organism=Caenorhabditis elegans, GI17535051, Length=298, Percent_Identity=48.993288590604, Blast_Score=277, Evalue=4e-75,
Organism=Caenorhabditis elegans, GI115535073, Length=305, Percent_Identity=49.8360655737705, Blast_Score=267, Evalue=6e-72,
Organism=Caenorhabditis elegans, GI17562970, Length=305, Percent_Identity=47.8688524590164, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI32566674, Length=299, Percent_Identity=48.1605351170569, Blast_Score=261, Evalue=4e-70,
Organism=Caenorhabditis elegans, GI17561720, Length=299, Percent_Identity=46.8227424749164, Blast_Score=235, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI32566672, Length=213, Percent_Identity=48.3568075117371, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17534315, Length=317, Percent_Identity=39.1167192429022, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25147552, Length=315, Percent_Identity=36.8253968253968, Blast_Score=160, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI17561716, Length=117, Percent_Identity=47.008547008547, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6321594, Length=318, Percent_Identity=38.9937106918239, Blast_Score=194, Evalue=1e-50,
Organism=Saccharomyces cerevisiae, GI6321449, Length=329, Percent_Identity=35.258358662614, Blast_Score=148, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24643623, Length=312, Percent_Identity=41.9871794871795, Blast_Score=214, Evalue=5e-56,
Organism=Drosophila melanogaster, GI20129101, Length=312, Percent_Identity=41.9871794871795, Blast_Score=214, Evalue=5e-56,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 32919; Mature: 32919

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVAQSVSELIGKTPIVKLNRIVESDSADIYLKLEFMNPGSSVKDRIALAMIEDAEKKGL
CCHHHHHHHHHCCCCCEEHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
LKEGDTIIEPTSGNTGIGLAMVAAAKGYKAILVMPETMSVERRNLLRAYGAELVLTPGPE
CCCCCEEEECCCCCCCCHHHEEECCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCC
GMGGAIRQATELAKEHGYFIPQQFKNQSNPEIHRLTTGPEIVEQMGDQLDAFIAGIGTGG
CCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCC
TITGAGEVLKEAYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVEVYDEII
CCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHH
QVKTEQAFEYARRVAKEEGILVGISSGAVIYAATEVAKKLGKGKKVLVIIPSNGERYLST
HHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEHHHHHHHHCCCCEEEEEECCCCCCEECC
PLYQFES
CCHHCCC
>Mature Secondary Structure
MRVAQSVSELIGKTPIVKLNRIVESDSADIYLKLEFMNPGSSVKDRIALAMIEDAEKKGL
CCHHHHHHHHHCCCCCEEHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHCCC
LKEGDTIIEPTSGNTGIGLAMVAAAKGYKAILVMPETMSVERRNLLRAYGAELVLTPGPE
CCCCCEEEECCCCCCCCHHHEEECCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCC
GMGGAIRQATELAKEHGYFIPQQFKNQSNPEIHRLTTGPEIVEQMGDQLDAFIAGIGTGG
CCCHHHHHHHHHHHHCCCCCCHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCC
TITGAGEVLKEAYKDIKIYAVEPADSPVLSGGKPGPHKIQGIGAGFVPETLDVEVYDEII
CCCCHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHH
QVKTEQAFEYARRVAKEEGILVGISSGAVIYAATEVAKKLGKGKKVLVIIPSNGERYLST
HHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEHHHHHHHHCCCCEEEEEECCCCCCEECC
PLYQFES
CCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377; 8755892; 9298659 [H]