Click here to switch to the map view.

The map label for this gene is pcrA [H]

Identifier: 46447235

GI number: 46447235

Start: 1915865

End: 1917871

Strand: Reverse

Name: pcrA [H]

Synonym: pc1601

Alternate gene names: 46447235

Gene position: 1917871-1915865 (Counterclockwise)

Preceding gene: 46447237

Following gene: 46447233

Centisome position: 79.43

GC content: 34.78

Gene sequence:

>2007_bases
ATGGAAAAAGAATTAGAAACTTTAAATCCAGAGCAGAAGCATGCAGTTAAGCTCATTAATGGAAGAGTATTAATTTTAGC
TGGAGCTGGAAGCGGAAAAACTCGCGTGTTAACAATGCGAATGGCTTATTTAATTCGTTATTTAAATGTTTCACCCAGAG
CTATTTTAGGCTTAACTTTTACAAATAAAGCGGCCGCAGAAATGCGTCATCGATTGGCAAATTTTGTAGATTCAAAATTA
GCCAAGCAAGTGACCTTGTGCACATTCCATGGTTTTTGTATGCAGATTCTGCGCCAAGACATTGCCAAGCTTGGTTATAC
AACTAAATTTAGTTTGTACGATGAACAAGATGTGCAACGATTAATTAACATGATTGTTCGCGATATTTTGCAGCATGAAG
GGGAACTTCCCTCTTTGACTTCTACACTCAACGCCATCCGGCATGCAAAAAATAAAGGAATTTCTCCTGAAAATATTGAA
ATTTCTGAGTCAAAATGGCACAATTCATTTGTTCAAGACGTTTATCGCCGTTTACAAGCAAGTATGAGAGCATATAATGC
TGTTGATTTTGACCATCTTTTGAGCTTGACTGTTGAGTTGCTCGAACGATTTCCGGATGTTTTAGAAGCTTATCAAGAAC
GATTTCGCTATATGATGATTGATGAATATCAAGACACGAATCCGATTCAATATCGACTTGCCTCTTTACTTACTTCTAAA
TACCAAAATTTATGTGTTGTTGGTGATGACGATCAGTCGATTTATGGTTGGCGAGGTGCAGATGTTAAAAATATTTTAGA
ATTTGAAAAAGCAACTGTCATTAAATTAGAACAGAATTATCGTTCGACAAATTTTATTTTAAAAGCTGCAAATGCAGTTA
TTGATCACAATCAATATCGACATAAAAAAAAATTATGGAGTGAGAAAGGAGAAGGACATCCTATTGAGGTCTTTCATACA
CCGAATGAGCTAGAAGAAGCTCAAGTAGTAGTTAAAAGAATTGTAAAAATGAAAGAAAGTTTAAATTTGCAGTGGAAAGA
CTTTGCCATTTTATATCGATCTAATGCTCTATCTCGCCAATTTGAAACGGCTCTCATGAGACAGGTTTGGAAATTTCAGA
ATCGGTGGATTCAAGGAATTCCTTATGAAATTTTTGGAGGCACAGAATTTTATGAACGAAAAGAAGTTAAAGACTTATGT
GCTTATTTGAGATTAATCATTAATCCGGCTGATCAAGAAGCTCTGCTACGAATTGTGAATCAACCAAGAAGAGGAATTGG
TGAAGATAGCTTAGATCTATTGACGACCCATAATAGGCAGTTTCAAAGGCCTCTTTGGGATGTTTTAAAAGAGGTTGCAA
ATCAAAAAGGGGAAGGAGCTAAACTAATTTATCATGGAAAAGCCTTTAAAGGAATTTGTGAGTTTACTCATTTAATTAGC
GAAGCAAAAAAACGATTCCAAGAAGGTAATTTAGCAGAAAATTTGAAATGGCTAATAGAGCAAATAAATTATCAAAAAGC
CATTAAAGAGGAAGTGAAAAGTCAACAAATGAGGGACTTTAAATGGGAAAATATTCAAGAATTTGTTTCTTCTTTAGCTG
AATTTGAACAGCAGGCACAACTCAACCCCGATTTAGAAAGCTCTTTAGAAAATTTTTTGGGAAATATGTATGTAGATAAT
AAATTTAATCAATCGACAAAAAATAATCGTCATGAAGACCGCGTAAGTTTACTTACATTTCATAGTGCAAAAGGGCTTGA
ATTTCCAGTTTGTTTTTTAGTTGGTATGGAAGATCATATCATTCCACATGAAAAAAGTATGAAAGAAACAGGAATCGAAG
AAGAAAGAAGGTTGATGTATGTTGCAATTACACGTGCTCAGCAGCATTTAACAATAAGTATGGCTCAGCAACGTAAACGT
ATGGGTAAGGATATGGCTAGCCGCCCATCGAGATTTTTATTTGAAATTCCGAAAGAGTTATTGAAAATGACTGACTGGAG
AAATTAA

Upstream 100 bases:

>100_bases
CTGTTGTTAATAAAAGACCGATCTGTCAACCTTTTGAATTGTTTTTGGTAAACTTAATCCTTATCTCATATGTAAATGAG
CAAAATTAGAAATTGTATCG

Downstream 100 bases:

>100_bases
AAAAATAATTCTGAATCCTGATTTTTTTATCGTGATCCAGAATCAACTTTAAATAATTATCGATAATAAGTGTTAAAGTG
GTTCATAGAACCCGAGAATT

Product: ATP-dependent DNA helicase, mutU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 668

Protein sequence:

>668_residues
MEKELETLNPEQKHAVKLINGRVLILAGAGSGKTRVLTMRMAYLIRYLNVSPRAILGLTFTNKAAAEMRHRLANFVDSKL
AKQVTLCTFHGFCMQILRQDIAKLGYTTKFSLYDEQDVQRLINMIVRDILQHEGELPSLTSTLNAIRHAKNKGISPENIE
ISESKWHNSFVQDVYRRLQASMRAYNAVDFDHLLSLTVELLERFPDVLEAYQERFRYMMIDEYQDTNPIQYRLASLLTSK
YQNLCVVGDDDQSIYGWRGADVKNILEFEKATVIKLEQNYRSTNFILKAANAVIDHNQYRHKKKLWSEKGEGHPIEVFHT
PNELEEAQVVVKRIVKMKESLNLQWKDFAILYRSNALSRQFETALMRQVWKFQNRWIQGIPYEIFGGTEFYERKEVKDLC
AYLRLIINPADQEALLRIVNQPRRGIGEDSLDLLTTHNRQFQRPLWDVLKEVANQKGEGAKLIYHGKAFKGICEFTHLIS
EAKKRFQEGNLAENLKWLIEQINYQKAIKEEVKSQQMRDFKWENIQEFVSSLAEFEQQAQLNPDLESSLENFLGNMYVDN
KFNQSTKNNRHEDRVSLLTFHSAKGLEFPVCFLVGMEDHIIPHEKSMKETGIEEERRLMYVAITRAQQHLTISMAQQRKR
MGKDMASRPSRFLFEIPKELLKMTDWRN

Sequences:

>Translated_668_residues
MEKELETLNPEQKHAVKLINGRVLILAGAGSGKTRVLTMRMAYLIRYLNVSPRAILGLTFTNKAAAEMRHRLANFVDSKL
AKQVTLCTFHGFCMQILRQDIAKLGYTTKFSLYDEQDVQRLINMIVRDILQHEGELPSLTSTLNAIRHAKNKGISPENIE
ISESKWHNSFVQDVYRRLQASMRAYNAVDFDHLLSLTVELLERFPDVLEAYQERFRYMMIDEYQDTNPIQYRLASLLTSK
YQNLCVVGDDDQSIYGWRGADVKNILEFEKATVIKLEQNYRSTNFILKAANAVIDHNQYRHKKKLWSEKGEGHPIEVFHT
PNELEEAQVVVKRIVKMKESLNLQWKDFAILYRSNALSRQFETALMRQVWKFQNRWIQGIPYEIFGGTEFYERKEVKDLC
AYLRLIINPADQEALLRIVNQPRRGIGEDSLDLLTTHNRQFQRPLWDVLKEVANQKGEGAKLIYHGKAFKGICEFTHLIS
EAKKRFQEGNLAENLKWLIEQINYQKAIKEEVKSQQMRDFKWENIQEFVSSLAEFEQQAQLNPDLESSLENFLGNMYVDN
KFNQSTKNNRHEDRVSLLTFHSAKGLEFPVCFLVGMEDHIIPHEKSMKETGIEEERRLMYVAITRAQQHLTISMAQQRKR
MGKDMASRPSRFLFEIPKELLKMTDWRN
>Mature_668_residues
MEKELETLNPEQKHAVKLINGRVLILAGAGSGKTRVLTMRMAYLIRYLNVSPRAILGLTFTNKAAAEMRHRLANFVDSKL
AKQVTLCTFHGFCMQILRQDIAKLGYTTKFSLYDEQDVQRLINMIVRDILQHEGELPSLTSTLNAIRHAKNKGISPENIE
ISESKWHNSFVQDVYRRLQASMRAYNAVDFDHLLSLTVELLERFPDVLEAYQERFRYMMIDEYQDTNPIQYRLASLLTSK
YQNLCVVGDDDQSIYGWRGADVKNILEFEKATVIKLEQNYRSTNFILKAANAVIDHNQYRHKKKLWSEKGEGHPIEVFHT
PNELEEAQVVVKRIVKMKESLNLQWKDFAILYRSNALSRQFETALMRQVWKFQNRWIQGIPYEIFGGTEFYERKEVKDLC
AYLRLIINPADQEALLRIVNQPRRGIGEDSLDLLTTHNRQFQRPLWDVLKEVANQKGEGAKLIYHGKAFKGICEFTHLIS
EAKKRFQEGNLAENLKWLIEQINYQKAIKEEVKSQQMRDFKWENIQEFVSSLAEFEQQAQLNPDLESSLENFLGNMYVDN
KFNQSTKNNRHEDRVSLLTFHSAKGLEFPVCFLVGMEDHIIPHEKSMKETGIEEERRLMYVAITRAQQHLTISMAQQRKR
MGKDMASRPSRFLFEIPKELLKMTDWRN

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=666, Percent_Identity=38.2882882882883, Blast_Score=419, Evalue=1e-118,
Organism=Escherichia coli, GI48994965, Length=662, Percent_Identity=37.3111782477341, Blast_Score=417, Evalue=1e-118,
Organism=Escherichia coli, GI1787196, Length=360, Percent_Identity=26.3888888888889, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6322369, Length=744, Percent_Identity=28.3602150537634, Blast_Score=194, Evalue=4e-50,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78130; Mature: 78130

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKELETLNPEQKHAVKLINGRVLILAGAGSGKTRVLTMRMAYLIRYLNVSPRAILGLTF
CCCCCHHCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEE
TNKAAAEMRHRLANFVDSKLAKQVTLCTFHGFCMQILRQDIAKLGYTTKFSLYDEQDVQR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHH
LINMIVRDILQHEGELPSLTSTLNAIRHAKNKGISPENIEISESKWHNSFVQDVYRRLQA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
SMRAYNAVDFDHLLSLTVELLERFPDVLEAYQERFRYMMIDEYQDTNPIQYRLASLLTSK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
YQNLCVVGDDDQSIYGWRGADVKNILEFEKATVIKLEQNYRSTNFILKAANAVIDHNQYR
HCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCHHHH
HKKKLWSEKGEGHPIEVFHTPNELEEAQVVVKRIVKMKESLNLQWKDFAILYRSNALSRQ
HHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHEEEEECCCHHHHH
FETALMRQVWKFQNRWIQGIPYEIFGGTEFYERKEVKDLCAYLRLIINPADQEALLRIVN
HHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QPRRGIGEDSLDLLTTHNRQFQRPLWDVLKEVANQKGEGAKLIYHGKAFKGICEFTHLIS
CCCCCCCCCCHHHHHHCCHHHCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHH
EAKKRFQEGNLAENLKWLIEQINYQKAIKEEVKSQQMRDFKWENIQEFVSSLAEFEQQAQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
LNPDLESSLENFLGNMYVDNKFNQSTKNNRHEDRVSLLTFHSAKGLEFPVCFLVGMEDHI
CCCCHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHEEEECCCCCCCCEEEEECCCCCC
IPHEKSMKETGIEEERRLMYVAITRAQQHLTISMAQQRKRMGKDMASRPSRFLFEIPKEL
CCCCHHHHHCCCCHHHHEEHEEEHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHH
LKMTDWRN
HHHCCCCC
>Mature Secondary Structure
MEKELETLNPEQKHAVKLINGRVLILAGAGSGKTRVLTMRMAYLIRYLNVSPRAILGLTF
CCCCCHHCCCCHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEE
TNKAAAEMRHRLANFVDSKLAKQVTLCTFHGFCMQILRQDIAKLGYTTKFSLYDEQDVQR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHH
LINMIVRDILQHEGELPSLTSTLNAIRHAKNKGISPENIEISESKWHNSFVQDVYRRLQA
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
SMRAYNAVDFDHLLSLTVELLERFPDVLEAYQERFRYMMIDEYQDTNPIQYRLASLLTSK
HHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
YQNLCVVGDDDQSIYGWRGADVKNILEFEKATVIKLEQNYRSTNFILKAANAVIDHNQYR
HCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHHHCCHHHH
HKKKLWSEKGEGHPIEVFHTPNELEEAQVVVKRIVKMKESLNLQWKDFAILYRSNALSRQ
HHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCHHEEEEECCCHHHHH
FETALMRQVWKFQNRWIQGIPYEIFGGTEFYERKEVKDLCAYLRLIINPADQEALLRIVN
HHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
QPRRGIGEDSLDLLTTHNRQFQRPLWDVLKEVANQKGEGAKLIYHGKAFKGICEFTHLIS
CCCCCCCCCCHHHHHHCCHHHCCHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHH
EAKKRFQEGNLAENLKWLIEQINYQKAIKEEVKSQQMRDFKWENIQEFVSSLAEFEQQAQ
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHC
LNPDLESSLENFLGNMYVDNKFNQSTKNNRHEDRVSLLTFHSAKGLEFPVCFLVGMEDHI
CCCCHHHHHHHHHCCCEECCCCCCCCCCCCCHHHHHHEEEECCCCCCCCEEEEECCCCCC
IPHEKSMKETGIEEERRLMYVAITRAQQHLTISMAQQRKRMGKDMASRPSRFLFEIPKEL
CCCCHHHHHCCCCHHHHEEHEEEHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHH
LKMTDWRN
HHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]