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The map label for this gene is ispE

Identifier: 46447223

GI number: 46447223

Start: 1905729

End: 1906595

Strand: Reverse

Name: ispE

Synonym: pc1589

Alternate gene names: 46447223

Gene position: 1906595-1905729 (Counterclockwise)

Preceding gene: 46447224

Following gene: 46447222

Centisome position: 78.97

GC content: 36.22

Gene sequence:

>867_bases
TTGCGCCTATCTATTATTGAGATAATAAATAGTATGTTTTCCATTCGATTATTTTCACCGGCAAAAATAAATTTATTTTT
AAAAGTTATTGGCAAAAGAGCGGATGGGTATCATGAGCTTTCCTCACTTTTCCAAACAATCTCTGCTGGGGATATTTTAA
CATTTCAAAGGCAGACAATAGATACGCTCACCTGTTCAGATCCTTATCTTCCCACCGATGATTCTAATTTAGTTCTTAAA
GCCATGCGTTTATTTCGTTCAAAAACTGGTTTGGATTTACACTTGCGCATTCATTTAGATAAACGACTTCCTTCTCAAGC
AGGACTTGGAGGAGGTAGCAGTAATGCTGCGACTACTCTTTGGGCTTGTAATCAACTAGCAGGAGAAATCGTAACAACAG
AAGAGTTAATGCAATGGGGATCCGAAATTGGGGCAGACATTCCTTTTTTCTTTTCCAAAGGAACTGCTCATTGTACTGGC
AGAGGAGAATGTGTAAATTCTTTAGAACCTTTAGCTCATTGTAAAATTTGGATTGTTAAGCCTCCTTTTGGACTTTCGAC
TCCAGAAGTTTATAAGCACTTAAATTTCTCTCAGCCTAATGAAAATAATAACGATTATGCAAGCTTTAAAGAAAAACCTT
ATTTTAATGATTTAGAAGCTTCGGCTTTTGAAATCAAACCTGAATTAAAAATACTAAAAAACACTCTTCTGTCTTCTGGT
TTTGATACAGTGCTTATGTCTGGGTCAGGATCTTCTTTTTTTTGTATTGGTCAAGGGCAAATTCCTGCTTCTTTTAAAGC
TTTTTCTGCGTACTTTATCAACCGTTCATCCAATCGTTGGTATTCTACGCTGCCCAAATTAACCTGA

Upstream 100 bases:

>100_bases
GTGATGTCCGAAGAAGGATACCGTACAAGTTTAGAAGAACAAAAAGCTTAAGGCGGCTTAAGCTTTCTTGTTCTAATTTG
TAAGGCGCAATTAAATTTAA

Downstream 100 bases:

>100_bases
CGCTTGTTCCATTAGCCTTAGGAATGCCAAATTTATTTTTCCAAAATTTAATTTTTAGTTCTTTCTTGTCTTATTTATGA
TAGACTATAGATTTATTTAT

Product: isopentenyl monophosphate kinase (IPK)

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 288; Mature: 288

Protein sequence:

>288_residues
MRLSIIEIINSMFSIRLFSPAKINLFLKVIGKRADGYHELSSLFQTISAGDILTFQRQTIDTLTCSDPYLPTDDSNLVLK
AMRLFRSKTGLDLHLRIHLDKRLPSQAGLGGGSSNAATTLWACNQLAGEIVTTEELMQWGSEIGADIPFFFSKGTAHCTG
RGECVNSLEPLAHCKIWIVKPPFGLSTPEVYKHLNFSQPNENNNDYASFKEKPYFNDLEASAFEIKPELKILKNTLLSSG
FDTVLMSGSGSSFFCIGQGQIPASFKAFSAYFINRSSNRWYSTLPKLT

Sequences:

>Translated_288_residues
MRLSIIEIINSMFSIRLFSPAKINLFLKVIGKRADGYHELSSLFQTISAGDILTFQRQTIDTLTCSDPYLPTDDSNLVLK
AMRLFRSKTGLDLHLRIHLDKRLPSQAGLGGGSSNAATTLWACNQLAGEIVTTEELMQWGSEIGADIPFFFSKGTAHCTG
RGECVNSLEPLAHCKIWIVKPPFGLSTPEVYKHLNFSQPNENNNDYASFKEKPYFNDLEASAFEIKPELKILKNTLLSSG
FDTVLMSGSGSSFFCIGQGQIPASFKAFSAYFINRSSNRWYSTLPKLT
>Mature_288_residues
MRLSIIEIINSMFSIRLFSPAKINLFLKVIGKRADGYHELSSLFQTISAGDILTFQRQTIDTLTCSDPYLPTDDSNLVLK
AMRLFRSKTGLDLHLRIHLDKRLPSQAGLGGGSSNAATTLWACNQLAGEIVTTEELMQWGSEIGADIPFFFSKGTAHCTG
RGECVNSLEPLAHCKIWIVKPPFGLSTPEVYKHLNFSQPNENNNDYASFKEKPYFNDLEASAFEIKPELKILKNTLLSSG
FDTVLMSGSGSSFFCIGQGQIPASFKAFSAYFINRSSNRWYSTLPKLT

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=244, Percent_Identity=31.5573770491803, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_PARUW (Q6MAT6)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_008588.1
- ProteinModelPortal:   Q6MAT6
- SMR:   Q6MAT6
- STRING:   Q6MAT6
- GeneID:   2780854
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc1589
- NMPDR:   fig|264201.1.peg.1589
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   DGYHNID
- PhylomeDB:   Q6MAT6
- BioCyc:   CPRO264201:PC1589-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376
- TIGRFAMs:   TIGR00154

Pfam domain/function: PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 32001; Mature: 32001

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: NA

Important sites: ACT_SITE 22-22 ACT_SITE 146-146

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLSIIEIINSMFSIRLFSPAKINLFLKVIGKRADGYHELSSLFQTISAGDILTFQRQTI
CCCHHHHHHHHHHEEEEECCCHHEEHEEEHHCCCCCHHHHHHHHHHHCCCCEEEEEHHHH
DTLTCSDPYLPTDDSNLVLKAMRLFRSKTGLDLHLRIHLDKRLPSQAGLGGGSSNAATTL
CEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHCCCCCCCCCCHHHH
WACNQLAGEIVTTEELMQWGSEIGADIPFFFSKGTAHCTGRGECVNSLEPLAHCKIWIVK
HHHHHHHCCEECHHHHHHHHHHHCCCCCEEECCCCEEECCCCHHHHHCCCHHHEEEEEEE
PPFGLSTPEVYKHLNFSQPNENNNDYASFKEKPYFNDLEASAFEIKPELKILKNTLLSSG
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCC
FDTVLMSGSGSSFFCIGQGQIPASFKAFSAYFINRSSNRWYSTLPKLT
CCEEEEECCCCEEEEEECCCCCCHHHHHEEEEEECCCCCHHHCCCCCC
>Mature Secondary Structure
MRLSIIEIINSMFSIRLFSPAKINLFLKVIGKRADGYHELSSLFQTISAGDILTFQRQTI
CCCHHHHHHHHHHEEEEECCCHHEEHEEEHHCCCCCHHHHHHHHHHHCCCCEEEEEHHHH
DTLTCSDPYLPTDDSNLVLKAMRLFRSKTGLDLHLRIHLDKRLPSQAGLGGGSSNAATTL
CEEECCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHCCCCCCCCCCHHHH
WACNQLAGEIVTTEELMQWGSEIGADIPFFFSKGTAHCTGRGECVNSLEPLAHCKIWIVK
HHHHHHHCCEECHHHHHHHHHHHCCCCCEEECCCCEEECCCCHHHHHCCCHHHEEEEEEE
PPFGLSTPEVYKHLNFSQPNENNNDYASFKEKPYFNDLEASAFEIKPELKILKNTLLSSG
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCC
FDTVLMSGSGSSFFCIGQGQIPASFKAFSAYFINRSSNRWYSTLPKLT
CCEEEEECCCCEEEEEECCCCCCHHHHHEEEEEECCCCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA