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The map label for this gene is pheS

Identifier: 46446339

GI number: 46446339

Start: 856903

End: 857943

Strand: Direct

Name: pheS

Synonym: pc0705

Alternate gene names: 46446339

Gene position: 856903-857943 (Clockwise)

Preceding gene: 46446338

Following gene: 46446340

Centisome position: 35.49

GC content: 39.1

Gene sequence:

>1041_bases
ATGAGGTCTTCTGTGCAGCCATTGATCAATGAAATTCGTCAGCAATTCCTCCATGATTTAGAACAAGTTCAAACTACACT
TGAACTTGAGCAGCTTAAGATAAAATTTTTAGGCAAAAAAGGGTCCTTACAAGGTCTCATGAAAGAGCTTAAAAATGTTG
AGCCTGATCAGCGACCTTTGGTTGGCAAATTCATCAACGAATTGAAAGAATTCATGTCTAATCATTGCGATGAATTAGAA
CATCAGCTAATTGCTAAAGAAGAAAATGCTCAGTTAGCTCACGAAACTATTGATGTTACATTGCCAGGGCGACAGCGATT
TGTTGGCCGAAAACATCCGTTGACTCAAGCGATGGATCAAATTATCCATATTCTGTCTAAGATGGGTTTTTCGGTTCAGT
ATGGGCCTGATATTGATACGGACTATTACAATTTCGAAATTCTCAACTTCCCTCCTGAGCATCCAGCAAGAGATATGCAA
GACACTTTTTATATCTCTCCTCATGTTTTGCTTCGTACTCATACGTCGAATATTCAAGCGCGAGCAATGGAGCTAAATAG
GCCCCCCATTCGTATTATTGCTCCTGGAAAAGTTTATCGTAATGAAACAATTACAGCTCGCTCACATGTCTTTTTTCATC
AAGTTGAAGCTGTTTATATCGATCAACATGTTTCGTTTGCCGATTTATTTGCGACGATGGATGAATTTTTGAAAAAATTG
TTTAAGCAAACTATTGAAACTCGTTATCGACCGAGCTATTTTCCTTTTGTGGAACCAGGGCTTGAAGTAGATATTAGTTG
CCTAGTTTGTGAAGGCAAAGGATGTCAACTTTGTAAGCACACAGGGTGGGTTGAAGTCGCAGGTGCTGGAATGATTCATC
CCGAAGTTCTCAAAAATGGGGGCATCGATCCTGAGCACTATTCAGGTTTTGCTTGGGGGATGGGACTAGAGCGTTTAGTT
ATGATGTTGAGAGGGATTCAAGATATTCGTTTATTCACAGAAAATGATCTAAGATTCTTACAACAATTTACTTTGTTGTA
A

Upstream 100 bases:

>100_bases
AGTTGATGTTATTTATGATATCTCTTGTTTAACTCTATTAAGTCTCATAAGTGACTTTGTTGTGTCGTTCGACATTAAAT
AAATGTTCTCTTATCTTCTG

Downstream 100 bases:

>100_bases
AAGCCAATTAGGACAAAGCATGCCTGATGCATTAGATTGGGTGGTTTCAAATGAAGAATCAGGCAGTAAACTGGTCTCGT
TTTTAAGCAAGCAGTTAAGC

Product: phenylalanine-tRNA ligase alpha chain

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 346; Mature: 346

Protein sequence:

>346_residues
MRSSVQPLINEIRQQFLHDLEQVQTTLELEQLKIKFLGKKGSLQGLMKELKNVEPDQRPLVGKFINELKEFMSNHCDELE
HQLIAKEENAQLAHETIDVTLPGRQRFVGRKHPLTQAMDQIIHILSKMGFSVQYGPDIDTDYYNFEILNFPPEHPARDMQ
DTFYISPHVLLRTHTSNIQARAMELNRPPIRIIAPGKVYRNETITARSHVFFHQVEAVYIDQHVSFADLFATMDEFLKKL
FKQTIETRYRPSYFPFVEPGLEVDISCLVCEGKGCQLCKHTGWVEVAGAGMIHPEVLKNGGIDPEHYSGFAWGMGLERLV
MMLRGIQDIRLFTENDLRFLQQFTLL

Sequences:

>Translated_346_residues
MRSSVQPLINEIRQQFLHDLEQVQTTLELEQLKIKFLGKKGSLQGLMKELKNVEPDQRPLVGKFINELKEFMSNHCDELE
HQLIAKEENAQLAHETIDVTLPGRQRFVGRKHPLTQAMDQIIHILSKMGFSVQYGPDIDTDYYNFEILNFPPEHPARDMQ
DTFYISPHVLLRTHTSNIQARAMELNRPPIRIIAPGKVYRNETITARSHVFFHQVEAVYIDQHVSFADLFATMDEFLKKL
FKQTIETRYRPSYFPFVEPGLEVDISCLVCEGKGCQLCKHTGWVEVAGAGMIHPEVLKNGGIDPEHYSGFAWGMGLERLV
MMLRGIQDIRLFTENDLRFLQQFTLL
>Mature_346_residues
MRSSVQPLINEIRQQFLHDLEQVQTTLELEQLKIKFLGKKGSLQGLMKELKNVEPDQRPLVGKFINELKEFMSNHCDELE
HQLIAKEENAQLAHETIDVTLPGRQRFVGRKHPLTQAMDQIIHILSKMGFSVQYGPDIDTDYYNFEILNFPPEHPARDMQ
DTFYISPHVLLRTHTSNIQARAMELNRPPIRIIAPGKVYRNETITARSHVFFHQVEAVYIDQHVSFADLFATMDEFLKKL
FKQTIETRYRPSYFPFVEPGLEVDISCLVCEGKGCQLCKHTGWVEVAGAGMIHPEVLKNGGIDPEHYSGFAWGMGLERLV
MMLRGIQDIRLFTENDLRFLQQFTLL

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=270, Percent_Identity=30.7407407407407, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI5729820, Length=241, Percent_Identity=30.2904564315353, Blast_Score=108, Evalue=8e-24,
Organism=Escherichia coli, GI1788007, Length=325, Percent_Identity=45.8461538461538, Blast_Score=305, Evalue=2e-84,
Organism=Caenorhabditis elegans, GI32563657, Length=270, Percent_Identity=30.3703703703704, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI17508957, Length=270, Percent_Identity=30.3703703703704, Blast_Score=115, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI32566635, Length=250, Percent_Identity=30, Blast_Score=101, Evalue=5e-22,
Organism=Saccharomyces cerevisiae, GI6321087, Length=300, Percent_Identity=28.6666666666667, Blast_Score=129, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=102, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI18858079, Length=271, Percent_Identity=30.2583025830258, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17137424, Length=254, Percent_Identity=27.5590551181102, Blast_Score=86, Evalue=4e-17,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_PARUW (Q6MDC0)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007704.1
- ProteinModelPortal:   Q6MDC0
- SMR:   Q6MDC0
- STRING:   Q6MDC0
- GeneID:   2779548
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0705
- NMPDR:   fig|264201.1.peg.705
- eggNOG:   COG0016
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- PhylomeDB:   Q6MDC0
- ProtClustDB:   CLSK2762234
- BioCyc:   CPRO264201:PC0705-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 40009; Mature: 40009

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSSVQPLINEIRQQFLHDLEQVQTTLELEQLKIKFLGKKGSLQGLMKELKNVEPDQRPL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCH
VGKFINELKEFMSNHCDELEHQLIAKEENAQLAHETIDVTLPGRQRFVGRKHPLTQAMDQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEEEEEECCHHHHCCCCCCHHHHHHH
IIHILSKMGFSVQYGPDIDTDYYNFEILNFPPEHPARDMQDTFYISPHVLLRTHTSNIQA
HHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCEEEEEECCCCCEE
RAMELNRPPIRIIAPGKVYRNETITARSHVFFHQVEAVYIDQHVSFADLFATMDEFLKKL
HHEECCCCCEEEEECCCEECCCCEEHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHH
FKQTIETRYRPSYFPFVEPGLEVDISCLVCEGKGCQLCKHTGWVEVAGAGMIHPEVLKNG
HHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHCCCEEEECCCCCCCHHHHHCC
GIDPEHYSGFAWGMGLERLVMMLRGIQDIRLFTENDLRFLQQFTLL
CCCCHHCCCHHHHHHHHHHHHHHHCHHHHHEECHHHHHHHHHHHCC
>Mature Secondary Structure
MRSSVQPLINEIRQQFLHDLEQVQTTLELEQLKIKFLGKKGSLQGLMKELKNVEPDQRPL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCH
VGKFINELKEFMSNHCDELEHQLIAKEENAQLAHETIDVTLPGRQRFVGRKHPLTQAMDQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEEEEEECCHHHHCCCCCCHHHHHHH
IIHILSKMGFSVQYGPDIDTDYYNFEILNFPPEHPARDMQDTFYISPHVLLRTHTSNIQA
HHHHHHHCCCEEEECCCCCCCCEEEEEECCCCCCCCCCCCCEEEECCEEEEEECCCCCEE
RAMELNRPPIRIIAPGKVYRNETITARSHVFFHQVEAVYIDQHVSFADLFATMDEFLKKL
HHEECCCCCEEEEECCCEECCCCEEHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHH
FKQTIETRYRPSYFPFVEPGLEVDISCLVCEGKGCQLCKHTGWVEVAGAGMIHPEVLKNG
HHHHHHHCCCCCCCCCCCCCCCEEEEEEEECCCCCCHHHCCCEEEECCCCCCCHHHHHCC
GIDPEHYSGFAWGMGLERLVMMLRGIQDIRLFTENDLRFLQQFTLL
CCCCHHCCCHHHHHHHHHHHHHHHCHHHHHEECHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA