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The map label for this gene is rlmN2 [H]

Identifier: 46446329

GI number: 46446329

Start: 846915

End: 847907

Strand: Direct

Name: rlmN2 [H]

Synonym: pc0695

Alternate gene names: 46446329

Gene position: 846915-847907 (Clockwise)

Preceding gene: 46446326

Following gene: 46446330

Centisome position: 35.08

GC content: 37.46

Gene sequence:

>993_bases
TTGAGTTGGATTTATCAAAAAGGGGTTTTGTCTTGGGATAAAATGTCTAATTTAAGCCAATCTTTACGAGAAAAACTAGC
GAAGCATATTAGATTACCCGTTTTAGAATTAGTGCGTTATACTGAATCAATTGATCAAGAAACTATTAAATTCTTATGGC
GTTTACGAGATGGAAATTTAGTAGAGTCTGTTTTAATTTTATCCGGAATTCGTCGAACTGTTTGCGTGTCTTCTCAGGTA
GGCTGCCCAGCTAAATGCGCCTTTTGTGCTTCTGGCCAGCAAGGTTTTTTTAGAAATCTTAGGCCGACTGAAATTATTGA
ACAGATTTTGCAAATTAATGCTTGGCTTTCTAGCAAAGGAGAAAAAGTTTCGCATGTTGTTTATATGGGAATGGGAGAGC
CGCTTAAAAATTATGAATCTGTTGTTGCCTCCATTAGGGTATTAAGTCACCCTGACTTTTGTAATATTTCTCAAAGAAGA
ATCACAGTTTCTACAGTTGGAGTCGTGGAAGGTATCAAACGCTTATCTAAAGAGGGATTAAAGGTTAATTTAGTCCTATC
TCTGCATGCTCCTAATCAACATATTCGTAAAAAAATTATCCCCTATGCTCGAAAATATCCTTTAGAAGAAATATTAGAGT
CAATGGATGAGTATGCTCAAAAAACTAAACGAGACATTACCTTCGAATACACTTTACTAGCGGGCATTAATGATCATCCC
GATCATGCTCATGAATTGGCACACTTATTGAAAGGGAAACAATGTACAGTTAATCTGATTCCTTATAACCCAATTCCAGG
ATTGCGGTTAAAGAGACCAGAGAAAAAAGCGATTAAACAGTTTCGAAGTGTCCTTTATGGGTCGCATATTGTCAATACAT
GCCGTTATACGAAAGGAGACGATATTGGAGCGGCTTGCGGTCAACTTGCCTTACAAGAACGAGAGGGGCAAACAGGGCTT
CCTATGTTAAAAGAAGTCGCATTTGGATCTTAA

Upstream 100 bases:

>100_bases
ACTAATATGGCTTATTATGGATTACGCAGATTTTGATCATCAAAAACTAGTTGAATGGCTTAAGGCACATGGAGAGAAAG
AGTTTCATGCTAAGCAAATT

Downstream 100 bases:

>100_bases
ATGAACATGCTATACGGGAATATATATTTATATTTTCTAATTTGATATAATAAATACATATGCTAAAATAATATTGATGT
ATATTGGTATTAAGTTGGTT

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase 2 [H]

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSWIYQKGVLSWDKMSNLSQSLREKLAKHIRLPVLELVRYTESIDQETIKFLWRLRDGNLVESVLILSGIRRTVCVSSQV
GCPAKCAFCASGQQGFFRNLRPTEIIEQILQINAWLSSKGEKVSHVVYMGMGEPLKNYESVVASIRVLSHPDFCNISQRR
ITVSTVGVVEGIKRLSKEGLKVNLVLSLHAPNQHIRKKIIPYARKYPLEEILESMDEYAQKTKRDITFEYTLLAGINDHP
DHAHELAHLLKGKQCTVNLIPYNPIPGLRLKRPEKKAIKQFRSVLYGSHIVNTCRYTKGDDIGAACGQLALQEREGQTGL
PMLKEVAFGS

Sequences:

>Translated_330_residues
MSWIYQKGVLSWDKMSNLSQSLREKLAKHIRLPVLELVRYTESIDQETIKFLWRLRDGNLVESVLILSGIRRTVCVSSQV
GCPAKCAFCASGQQGFFRNLRPTEIIEQILQINAWLSSKGEKVSHVVYMGMGEPLKNYESVVASIRVLSHPDFCNISQRR
ITVSTVGVVEGIKRLSKEGLKVNLVLSLHAPNQHIRKKIIPYARKYPLEEILESMDEYAQKTKRDITFEYTLLAGINDHP
DHAHELAHLLKGKQCTVNLIPYNPIPGLRLKRPEKKAIKQFRSVLYGSHIVNTCRYTKGDDIGAACGQLALQEREGQTGL
PMLKEVAFGS
>Mature_329_residues
SWIYQKGVLSWDKMSNLSQSLREKLAKHIRLPVLELVRYTESIDQETIKFLWRLRDGNLVESVLILSGIRRTVCVSSQVG
CPAKCAFCASGQQGFFRNLRPTEIIEQILQINAWLSSKGEKVSHVVYMGMGEPLKNYESVVASIRVLSHPDFCNISQRRI
TVSTVGVVEGIKRLSKEGLKVNLVLSLHAPNQHIRKKIIPYARKYPLEEILESMDEYAQKTKRDITFEYTLLAGINDHPD
HAHELAHLLKGKQCTVNLIPYNPIPGLRLKRPEKKAIKQFRSVLYGSHIVNTCRYTKGDDIGAACGQLALQEREGQTGLP
MLKEVAFGS

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=318, Percent_Identity=40.5660377358491, Blast_Score=230, Evalue=7e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 37324; Mature: 37193

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSWIYQKGVLSWDKMSNLSQSLREKLAKHIRLPVLELVRYTESIDQETIKFLWRLRDGNL
CCCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
VESVLILSGIRRTVCVSSQVGCPAKCAFCASGQQGFFRNLRPTEIIEQILQINAWLSSKG
HHHHHHHHHHHHHHEECCCCCCCCHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCC
EKVSHVVYMGMGEPLKNYESVVASIRVLSHPDFCNISQRRITVSTVGVVEGIKRLSKEGL
CCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCC
KVNLVLSLHAPNQHIRKKIIPYARKYPLEEILESMDEYAQKTKRDITFEYTLLAGINDHP
EEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCC
DHAHELAHLLKGKQCTVNLIPYNPIPGLRLKRPEKKAIKQFRSVLYGSHIVNTCRYTKGD
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DIGAACGQLALQEREGQTGLPMLKEVAFGS
CHHHHHHHHHHHHCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SWIYQKGVLSWDKMSNLSQSLREKLAKHIRLPVLELVRYTESIDQETIKFLWRLRDGNL
CCCHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCH
VESVLILSGIRRTVCVSSQVGCPAKCAFCASGQQGFFRNLRPTEIIEQILQINAWLSSKG
HHHHHHHHHHHHHHEECCCCCCCCHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHCCCC
EKVSHVVYMGMGEPLKNYESVVASIRVLSHPDFCNISQRRITVSTVGVVEGIKRLSKEGL
CCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHHCCC
KVNLVLSLHAPNQHIRKKIIPYARKYPLEEILESMDEYAQKTKRDITFEYTLLAGINDHP
EEEEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCC
DHAHELAHLLKGKQCTVNLIPYNPIPGLRLKRPEKKAIKQFRSVLYGSHIVNTCRYTKGD
HHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
DIGAACGQLALQEREGQTGLPMLKEVAFGS
CHHHHHHHHHHHHCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA