Click here to switch to the map view.

The map label for this gene is trmD

Identifier: 46446290

GI number: 46446290

Start: 802349

End: 803017

Strand: Direct

Name: trmD

Synonym: pc0656

Alternate gene names: 46446290

Gene position: 802349-803017 (Clockwise)

Preceding gene: 46446289

Following gene: 46446291

Centisome position: 33.23

GC content: 41.26

Gene sequence:

>669_bases
GTGATCGTTGATATTTTGTCTCTTTTCCCAGGGTATTTTAAAGGGCCTTTCGATGAAAGTATCCTTAAACGGGCCCAAGA
AAAGGGACTATTAACGATTCGCTTAACTGATATACGGGATTTTGCTGATAATCGCTTTCATCGCGTAGACGATAGACCTT
ATGGAGGTGGGCCTGGAATGGTCATGATGCCGCAGCCAGTTACAATGGCCATTCAACATGTGAAAACTCCAGAAGCGAAA
GTCATTTATTTGTCCCCCCAAGGGAGAACATTAAATGCAGTTAAATGTCGACAACTAGCCTTGGAAAAGCATCTTATTCT
TTTATGTGGTCACTATGAAGGAATAGATCAGCGCGTTTTAGATGAAGAAGTAGACGAAGAAATTAGTATTGGAGATTACG
TCTTGACTAACGGTTGTTTAGCAGCTATTGTTCTTCTAGATGCTTTTTCCCGCTTTATTCCAGGTGTATTAGGCCATCCT
TCAGCGGCAGCAGAAGATTCTTTTGAAGGAGGGTTATTAGATTATCCTCACTACACAAGACCCGAAGTTTTTCAAGGGTG
TTCTGTACCAAATGTTTTGTTAAGTGGGAATCATCAAGAAATTGCCAAGTGGCGTCATGAGCAAGCGCTAAAAAAGACGC
AAGAGATAAGACCAGATTTGCTTGCTTAA

Upstream 100 bases:

>100_bases
TAAGAAAACTTCTCCAGAAATTATTCGTGCTCAAACAGAGAAAAAATTAAACTCTCTCGCAAAGGCAGCAACAAAACGAA
AAAAAAAGGACTAAATTTAG

Downstream 100 bases:

>100_bases
TTGAGTCAAAAACTTAAAAATGGTTTGCATAACATCGGTTGAAAAAAATAAACCTTTTCAAACGAAGTAAAAGAGTGAAA
TTTAGGAGAAATTGATCATG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MIVDILSLFPGYFKGPFDESILKRAQEKGLLTIRLTDIRDFADNRFHRVDDRPYGGGPGMVMMPQPVTMAIQHVKTPEAK
VIYLSPQGRTLNAVKCRQLALEKHLILLCGHYEGIDQRVLDEEVDEEISIGDYVLTNGCLAAIVLLDAFSRFIPGVLGHP
SAAAEDSFEGGLLDYPHYTRPEVFQGCSVPNVLLSGNHQEIAKWRHEQALKKTQEIRPDLLA

Sequences:

>Translated_222_residues
MIVDILSLFPGYFKGPFDESILKRAQEKGLLTIRLTDIRDFADNRFHRVDDRPYGGGPGMVMMPQPVTMAIQHVKTPEAK
VIYLSPQGRTLNAVKCRQLALEKHLILLCGHYEGIDQRVLDEEVDEEISIGDYVLTNGCLAAIVLLDAFSRFIPGVLGHP
SAAAEDSFEGGLLDYPHYTRPEVFQGCSVPNVLLSGNHQEIAKWRHEQALKKTQEIRPDLLA
>Mature_222_residues
MIVDILSLFPGYFKGPFDESILKRAQEKGLLTIRLTDIRDFADNRFHRVDDRPYGGGPGMVMMPQPVTMAIQHVKTPEAK
VIYLSPQGRTLNAVKCRQLALEKHLILLCGHYEGIDQRVLDEEVDEEISIGDYVLTNGCLAAIVLLDAFSRFIPGVLGHP
SAAAEDSFEGGLLDYPHYTRPEVFQGCSVPNVLLSGNHQEIAKWRHEQALKKTQEIRPDLLA

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=224, Percent_Identity=55.8035714285714, Blast_Score=241, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_PARUW (Q6MDG9)

Other databases:

- EMBL:   BX908798
- RefSeq:   YP_007655.1
- ProteinModelPortal:   Q6MDG9
- SMR:   Q6MDG9
- STRING:   Q6MDG9
- GeneID:   2780209
- GenomeReviews:   BX908798_GR
- KEGG:   pcu:pc0656
- NMPDR:   fig|264201.1.peg.656
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q6MDG9
- ProtClustDB:   CLSK2762212
- BioCyc:   CPRO264201:PC0656-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 24836; Mature: 24836

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: BINDING 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVDILSLFPGYFKGPFDESILKRAQEKGLLTIRLTDIRDFADNRFHRVDDRPYGGGPGM
CEEEHHHHCCHHHCCCCCHHHHHHHHHCCEEEEEECCHHHHHCCCCEECCCCCCCCCCCE
VMMPQPVTMAIQHVKTPEAKVIYLSPQGRTLNAVKCRQLALEKHLILLCGHYEGIDQRVL
EECCCHHHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHHHHCEEEEECCCCCHHHHHH
DEEVDEEISIGDYVLTNGCLAAIVLLDAFSRFIPGVLGHPSAAAEDSFEGGLLDYPHYTR
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCC
PEVFQGCSVPNVLLSGNHQEIAKWRHEQALKKTQEIRPDLLA
HHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MIVDILSLFPGYFKGPFDESILKRAQEKGLLTIRLTDIRDFADNRFHRVDDRPYGGGPGM
CEEEHHHHCCHHHCCCCCHHHHHHHHHCCEEEEEECCHHHHHCCCCEECCCCCCCCCCCE
VMMPQPVTMAIQHVKTPEAKVIYLSPQGRTLNAVKCRQLALEKHLILLCGHYEGIDQRVL
EECCCHHHHHHHHCCCCCCEEEEECCCCCEEHHHHHHHHHHHHCEEEEECCCCCHHHHHH
DEEVDEEISIGDYVLTNGCLAAIVLLDAFSRFIPGVLGHPSAAAEDSFEGGLLDYPHYTR
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCCCCCCC
PEVFQGCSVPNVLLSGNHQEIAKWRHEQALKKTQEIRPDLLA
HHHHCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA