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The map label for this gene is nuoI [H]

Identifier: 46446201

GI number: 46446201

Start: 701487

End: 701918

Strand: Direct

Name: nuoI [H]

Synonym: pc0567

Alternate gene names: 46446201

Gene position: 701487-701918 (Clockwise)

Preceding gene: 46446200

Following gene: 46446202

Centisome position: 29.05

GC content: 39.81

Gene sequence:

>432_bases
TTGATTATCGTTTTAAAACACGCTTTTCAAACTCCGGTCACACTGCGTTATCCTGAAGAAAAACGAATTTTACCAGCCAG
GTCTCGAGGTAGGCATTATTTAACAAAATGGAATGATGGTTTGGAAAGGTGTGTTGGTTGCGAACTTTGTGCTATCGTTT
GCCCAGCCCAGGCTATTTATGTGAAACCAGCTGCTAATGAACCGGGTCATATCCATTCTCATGGGGAGCGATATGCTTCT
GATTTTCAGATTAATATGTTGCGATGCATTTTTTGTGGTTATTGTGAAGAAGCTTGTCCAACTGGAGCAATTGTTTTAAG
TAATCAATATGAACTTTCAGCCTACACTAGAGAAGATATGATTTATACTAAAGATCGATTAACAGAGAAAACACCCGGAG
AATCCGGACGCGATCCTAGTAGGGAGATTTAA

Upstream 100 bases:

>100_bases
TTGATTCCCATTGCCATCTTAAATCTAATTATAACAGCATACTTTACGTTGGTGTAGCATGAGAACAACTATACACAAAG
TTATCACAATGATGAAAGGG

Downstream 100 bases:

>100_bases
GTCATGATGACCTCTTTAGCTTCACCTGAACAATGGATTTTTGGAATTTTACTTTTAATCTCTTCTTTCGGAATTCTTCT
CACATCAAAACCTATTCAAG

Product: NADH-ubiquinone oxidoreductase chain I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I [H]

Number of amino acids: Translated: 143; Mature: 143

Protein sequence:

>143_residues
MIIVLKHAFQTPVTLRYPEEKRILPARSRGRHYLTKWNDGLERCVGCELCAIVCPAQAIYVKPAANEPGHIHSHGERYAS
DFQINMLRCIFCGYCEEACPTGAIVLSNQYELSAYTREDMIYTKDRLTEKTPGESGRDPSREI

Sequences:

>Translated_143_residues
MIIVLKHAFQTPVTLRYPEEKRILPARSRGRHYLTKWNDGLERCVGCELCAIVCPAQAIYVKPAANEPGHIHSHGERYAS
DFQINMLRCIFCGYCEEACPTGAIVLSNQYELSAYTREDMIYTKDRLTEKTPGESGRDPSREI
>Mature_143_residues
MIIVLKHAFQTPVTLRYPEEKRILPARSRGRHYLTKWNDGLERCVGCELCAIVCPAQAIYVKPAANEPGHIHSHGERYAS
DFQINMLRCIFCGYCEEACPTGAIVLSNQYELSAYTREDMIYTKDRLTEKTPGESGRDPSREI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Homo sapiens, GI4505371, Length=125, Percent_Identity=41.6, Blast_Score=109, Evalue=9e-25,
Organism=Escherichia coli, GI1788617, Length=128, Percent_Identity=46.875, Blast_Score=114, Evalue=2e-27,
Organism=Escherichia coli, GI1789075, Length=122, Percent_Identity=33.6065573770492, Blast_Score=65, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17555194, Length=125, Percent_Identity=42.4, Blast_Score=110, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17864306, Length=125, Percent_Identity=40.8, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226 [H]

Pfam domain/function: PF00037 Fer4 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 16281; Mature: 16281

Theoretical pI: Translated: 7.36; Mature: 7.36

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
7.7 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
7.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIVLKHAFQTPVTLRYPEEKRILPARSRGRHYLTKWNDGLERCVGCELCAIVCPAQAIY
CEEEEEECCCCCEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHCHHHHEEECCCEEEE
VKPAANEPGHIHSHGERYASDFQINMLRCIFCGYCEEACPTGAIVLSNQYELSAYTREDM
EECCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCE
IYTKDRLTEKTPGESGRDPSREI
EEEHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MIIVLKHAFQTPVTLRYPEEKRILPARSRGRHYLTKWNDGLERCVGCELCAIVCPAQAIY
CEEEEEECCCCCEEEECCCCCCCCCCCCCCCEEEEHHHHHHHHHHCHHHHEEECCCEEEE
VKPAANEPGHIHSHGERYASDFQINMLRCIFCGYCEEACPTGAIVLSNQYELSAYTREDM
EECCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECCCCE
IYTKDRLTEKTPGESGRDPSREI
EEEHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA