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The map label for this gene is oprK [H]

Identifier: 46446186

GI number: 46446186

Start: 686609

End: 688078

Strand: Direct

Name: oprK [H]

Synonym: pc0552

Alternate gene names: 46446186

Gene position: 686609-688078 (Clockwise)

Preceding gene: 46446185

Following gene: 46446191

Centisome position: 28.44

GC content: 38.84

Gene sequence:

>1470_bases
GTGACGAGTTGCTTACTCCTTGCACTTAGTTCTTGCGTACTCCATCCTCCTTATCAACAACCTTGTTTGGAGATTCCTAA
AAAATGGCGTACTCCTTTTGAAGAAACTTCAACAGCTGCCAATTTTAGATGGTGGGAGGCCTTTGACGACCCTATTTTGA
AAAAACTCATCTTTGAGTCTTTGGAAAATAATAAGGATTTAAAGCTGGCTCTCGCGCGTACTGCTGAATTTAAAGCAAGA
TTGGGAATTGTTCGTTCTCAATTTTTTCCACAAATTAGCGGAGTAGGTCTTGCTTCACGTCAACGCACTCCAACAGAACT
ATCTGGAGGGAAAACTTCTTCAGGCGTTTCTTCTCAAGGTCAACCAGGTTTAACTCCTGTAGGAACAAACTTAATTCCTT
CTCCTTATACCAATAATTATCGTGTAGTTGCAGAAGCTGCTTATGAATTAGATTTATGGGGGCGAGTTTATAGCTCTTCA
GAAGCCGCTTTGGCAGATTTATTAGGACAGGTACAAGCTCGCAGGGCTCTGGTGCTATCTATTGTAAGTTCTGTTGCTAC
AAGCTATATTCAAATGAGACAATTTGATCAGCAATTGATTGTCTCTAAACAGACAGTTACTTCTAGGAAAGAATCTTACG
AATTGGCAAAACTTCGTTTTGAAGGAGGGCTGACGTCTGAGTTAGAAGTTAAGCAAGCAGCTGCTGAAGTGGATGAAGCA
ATTGCTGAAGTTTTTCGATTAGAAGTTTTAATTCCCCAACAAGAAAATTTAATTAGCATTTTAATTGGGCACAGCCCAAG
AGAAATTGAAAGAGGATTGAATATTAATGAATGGCCAACTCCACTTGCTGTGCCAGCCGGACTGCCCTCAGAGCTATTAG
AACAACGTCCCGATATTATACAAGCTGAGCAAGGATTAATTGCTGCGAATGCTCGAATTGGAGAAGCAAGAGCACAGTAT
TTTCCTAGTATTTCTTTAACAGGTTATTTTGGGTCTGAAAGTCTTCAACTTCATCATCTTTTTACCTCTCCCACAAGAGC
CTGGCAGTATGCAGCCAATCTTTTACAACCAATCTTTACAGGAGGAAAGATTTCGAGTGGCATCGATGTAGCGAAAGCGC
AAAAAAGGATTGCGTATTACAATTATCAACAAACAGTTTTGAATGCTTTTAAAGAAGTAGATAATGCTTTAATTGCTCAT
CAAAAATCCCGAGAATTACTTGAGGTACAAAAAAATAGTGTTGTTGTTTTAAAAGATTACCTACATTTAGCTAAATTACA
ATACGATAATGGGCAGACAGATTATTTGAATGTTTTGGACGCTGAACGCAAACTGTTTACAGCTCAACTCAACCTAGCTC
AAGCTGAAGCTGATGTTTTTTTAACATTAGTTAACGTTTATAAAGTATTAGGAGGGGGATGGGTCATTGATGCGGAAAAT
CTAAGTAAAGAAAAGCAGGAATGTTTGTAG

Upstream 100 bases:

>100_bases
GCTGCGACTATACTTGCCGTATTTTTAGTTCCTCTACTTTATAAACTTATTGATCAACGTATAGAAAAAAAAGAGAAGAG
AGAGCATGTTTAGAATTTTA

Downstream 100 bases:

>100_bases
TACAATCGATGCTGAACATATCAACATCGATTTGGAGATGATTATTTCGTTACTGCTGAATCTGTAGAAAGTGAGTTAAA
ATTCTTAACTGGTAAGTTAA

Product: putative outer membrane protein, componenet of multidrug efflux systems

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTSCLLLALSSCVLHPPYQQPCLEIPKKWRTPFEETSTAANFRWWEAFDDPILKKLIFESLENNKDLKLALARTAEFKAR
LGIVRSQFFPQISGVGLASRQRTPTELSGGKTSSGVSSQGQPGLTPVGTNLIPSPYTNNYRVVAEAAYELDLWGRVYSSS
EAALADLLGQVQARRALVLSIVSSVATSYIQMRQFDQQLIVSKQTVTSRKESYELAKLRFEGGLTSELEVKQAAAEVDEA
IAEVFRLEVLIPQQENLISILIGHSPREIERGLNINEWPTPLAVPAGLPSELLEQRPDIIQAEQGLIAANARIGEARAQY
FPSISLTGYFGSESLQLHHLFTSPTRAWQYAANLLQPIFTGGKISSGIDVAKAQKRIAYYNYQQTVLNAFKEVDNALIAH
QKSRELLEVQKNSVVVLKDYLHLAKLQYDNGQTDYLNVLDAERKLFTAQLNLAQAEADVFLTLVNVYKVLGGGWVIDAEN
LSKEKQECL

Sequences:

>Translated_489_residues
MTSCLLLALSSCVLHPPYQQPCLEIPKKWRTPFEETSTAANFRWWEAFDDPILKKLIFESLENNKDLKLALARTAEFKAR
LGIVRSQFFPQISGVGLASRQRTPTELSGGKTSSGVSSQGQPGLTPVGTNLIPSPYTNNYRVVAEAAYELDLWGRVYSSS
EAALADLLGQVQARRALVLSIVSSVATSYIQMRQFDQQLIVSKQTVTSRKESYELAKLRFEGGLTSELEVKQAAAEVDEA
IAEVFRLEVLIPQQENLISILIGHSPREIERGLNINEWPTPLAVPAGLPSELLEQRPDIIQAEQGLIAANARIGEARAQY
FPSISLTGYFGSESLQLHHLFTSPTRAWQYAANLLQPIFTGGKISSGIDVAKAQKRIAYYNYQQTVLNAFKEVDNALIAH
QKSRELLEVQKNSVVVLKDYLHLAKLQYDNGQTDYLNVLDAERKLFTAQLNLAQAEADVFLTLVNVYKVLGGGWVIDAEN
LSKEKQECL
>Mature_488_residues
TSCLLLALSSCVLHPPYQQPCLEIPKKWRTPFEETSTAANFRWWEAFDDPILKKLIFESLENNKDLKLALARTAEFKARL
GIVRSQFFPQISGVGLASRQRTPTELSGGKTSSGVSSQGQPGLTPVGTNLIPSPYTNNYRVVAEAAYELDLWGRVYSSSE
AALADLLGQVQARRALVLSIVSSVATSYIQMRQFDQQLIVSKQTVTSRKESYELAKLRFEGGLTSELEVKQAAAEVDEAI
AEVFRLEVLIPQQENLISILIGHSPREIERGLNINEWPTPLAVPAGLPSELLEQRPDIIQAEQGLIAANARIGEARAQYF
PSISLTGYFGSESLQLHHLFTSPTRAWQYAANLLQPIFTGGKISSGIDVAKAQKRIAYYNYQQTVLNAFKEVDNALIAHQ
KSRELLEVQKNSVVVLKDYLHLAKLQYDNGQTDYLNVLDAERKLFTAQLNLAQAEADVFLTLVNVYKVLGGGWVIDAENL
SKEKQECL

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=475, Percent_Identity=30.9473684210526, Blast_Score=226, Evalue=3e-60,
Organism=Escherichia coli, GI2367348, Length=474, Percent_Identity=23.8396624472574, Blast_Score=120, Evalue=3e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54359; Mature: 54227

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSCLLLALSSCVLHPPYQQPCLEIPKKWRTPFEETSTAANFRWWEAFDDPILKKLIFES
CCHHHHHHHHHHHCCCCCCCHHHHCCHHHCCCHHHCCCCCCCCHHHHHCHHHHHHHHHHH
LENNKDLKLALARTAEFKARLGIVRSQFFPQISGVGLASRQRTPTELSGGKTSSGVSSQG
HCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QPGLTPVGTNLIPSPYTNNYRVVAEAAYELDLWGRVYSSSEAALADLLGQVQARRALVLS
CCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IVSSVATSYIQMRQFDQQLIVSKQTVTSRKESYELAKLRFEGGLTSELEVKQAAAEVDEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHH
IAEVFRLEVLIPQQENLISILIGHSPREIERGLNINEWPTPLAVPAGLPSELLEQRPDII
HHHHHHHHEECCCCCCEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCE
QAEQGLIAANARIGEARAQYFPSISLTGYFGSESLQLHHLFTSPTRAWQYAANLLQPIFT
ECCCCEEEECCCHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHC
GGKISSGIDVAKAQKRIAYYNYQQTVLNAFKEVDNALIAHQKSRELLEVQKNSVVVLKDY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
LHLAKLQYDNGQTDYLNVLDAERKLFTAQLNLAQAEADVFLTLVNVYKVLGGGWVIDAEN
HHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHH
LSKEKQECL
HHHHHHHCC
>Mature Secondary Structure 
TSCLLLALSSCVLHPPYQQPCLEIPKKWRTPFEETSTAANFRWWEAFDDPILKKLIFES
CHHHHHHHHHHHCCCCCCCHHHHCCHHHCCCHHHCCCCCCCCHHHHHCHHHHHHHHHHH
LENNKDLKLALARTAEFKARLGIVRSQFFPQISGVGLASRQRTPTELSGGKTSSGVSSQG
HCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QPGLTPVGTNLIPSPYTNNYRVVAEAAYELDLWGRVYSSSEAALADLLGQVQARRALVLS
CCCCCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IVSSVATSYIQMRQFDQQLIVSKQTVTSRKESYELAKLRFEGGLTSELEVKQAAAEVDEA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHH
IAEVFRLEVLIPQQENLISILIGHSPREIERGLNINEWPTPLAVPAGLPSELLEQRPDII
HHHHHHHHEECCCCCCEEEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCE
QAEQGLIAANARIGEARAQYFPSISLTGYFGSESLQLHHLFTSPTRAWQYAANLLQPIFT
ECCCCEEEECCCHHHHHHHHCCCEEEEEEECCCCEEEEEEECCCHHHHHHHHHHHHHHHC
GGKISSGIDVAKAQKRIAYYNYQQTVLNAFKEVDNALIAHQKSRELLEVQKNSVVVLKDY
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
LHLAKLQYDNGQTDYLNVLDAERKLFTAQLNLAQAEADVFLTLVNVYKVLGGGWVIDAEN
HHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHH
LSKEKQECL
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]