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The map label for this gene is acrA [H]

Identifier: 46446184

GI number: 46446184

Start: 682304

End: 683491

Strand: Direct

Name: acrA [H]

Synonym: pc0550

Alternate gene names: 46446184

Gene position: 682304-683491 (Clockwise)

Preceding gene: 46446183

Following gene: 46446185

Centisome position: 28.26

GC content: 38.05

Gene sequence:

>1188_bases
GTGTTCGATTCGAAAGAGAAAAGTTTAAGAGAGATTTTAATGCCGAAAAAAAATTGTTTTTTTTATTACTTCTGTTTCTT
TTTAGCGAGTTTATGTTTAAGTTCTTGCGAAAAAGAAAAAACAACGAATTTTCAAGCCCCGTCAACAGATGTAAGTATTC
AAATCGTCGAACCAAAAACGGTACCTGCTGTTTTTGAGTTTGTTGGAATCGCTCAAAGTTCTCACGTAGTTGAAATTCGA
GCCCGAATTGAAGGATATTTAGATAAAATAGCTTATACGGAAGGAAGTTTTGTTCAAAAAGGAAATCTCCTCTTTCAAAT
TGATCCCAGGCCTTTTGAAGCTGCGCTGGAAAAAGCTACCGCACAATTAGCCAGAGAAGAAGCTGTTTTATGGGATGCAA
AACGGGCAGTAGATCGCTATAAACCCCTTTATGAGAAAAAAGCTGCAAGTTTAAGAGATTTGGATAATGCAATTGCTCGT
CAGCTGTCTTCCGAAGCTGATGTTCAAGCTGCAAAAGCTCAATTGAAAGAAGCGGATATTAACTTAGGATATACGACTAT
CTATGCTCCAGTTTCAGGTCAAGTAGGACAAGCTAATTACAGAGAGGGAGCTCTCATCGCTCCAACTGAAGATCAATTAG
CCACTCTTTCTGTGATTGATCCTATTTGGATTAATTTTAGTATCTCAGAAGGGGATATCTTACGTAGTCAAAAGTCTATT
TCGCAAGGGAAGCTTCATTTTCCGGCTTATGATAATTTCACGGTTCAGGTTATTTTAGCAGATCAAACCGTTTTTCCTGA
AATTGGGAAAGTTAATTTTTCCTCTCCTACTTATGATCAAAAAACAGGGACAATGATGGTTAGAGCTGTTTTGCCTAATC
CAAAAAATATCCTCCGTCCTGGTCAATTTGTACGAGTCCAATTACTTGGAGCAACCCGTCCAAACGCCATTCTTGTTCCG
CAAAAAGCTGTGCTTCAAAGCCAAAAGGGAATGTTTGTATTTTTGGTGAATAAGGATGATGAAGTTGTCATGCAACCTAT
AGAGCCCGGGGTATGGGATCAACAAAATTGGATTATTGATGCGGGTTTAAAGGCTGGAGATCAAGTCGTTGTGGATGGAG
TGAATAAATTAAGACCTGGAATGAAAGTCTCTATTAAAAAAAAAATTTTTGCTGAGAAAGCATCATGA

Upstream 100 bases:

>100_bases
AAATATGGATTTATTTGCTGGGAAATTACTAGAGTATTTTTTAAATTTTTTTGTTTTTCGCATTTAAACCCTAAACTCTG
GTTATGAGATTCCCCTAAAA

Downstream 100 bases:

>100_bases
TTTCCCATTTTTTTATCGACCGCCCTATTTTTGCTTTCGTTCTTTCTATCATTATTACCTTAGCAGGCTTATTTGCACTG
CGAATGTTACCGATCGAGCA

Product: putative multidrug-efflux transport protein acrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MFDSKEKSLREILMPKKNCFFYYFCFFLASLCLSSCEKEKTTNFQAPSTDVSIQIVEPKTVPAVFEFVGIAQSSHVVEIR
ARIEGYLDKIAYTEGSFVQKGNLLFQIDPRPFEAALEKATAQLAREEAVLWDAKRAVDRYKPLYEKKAASLRDLDNAIAR
QLSSEADVQAAKAQLKEADINLGYTTIYAPVSGQVGQANYREGALIAPTEDQLATLSVIDPIWINFSISEGDILRSQKSI
SQGKLHFPAYDNFTVQVILADQTVFPEIGKVNFSSPTYDQKTGTMMVRAVLPNPKNILRPGQFVRVQLLGATRPNAILVP
QKAVLQSQKGMFVFLVNKDDEVVMQPIEPGVWDQQNWIIDAGLKAGDQVVVDGVNKLRPGMKVSIKKKIFAEKAS

Sequences:

>Translated_395_residues
MFDSKEKSLREILMPKKNCFFYYFCFFLASLCLSSCEKEKTTNFQAPSTDVSIQIVEPKTVPAVFEFVGIAQSSHVVEIR
ARIEGYLDKIAYTEGSFVQKGNLLFQIDPRPFEAALEKATAQLAREEAVLWDAKRAVDRYKPLYEKKAASLRDLDNAIAR
QLSSEADVQAAKAQLKEADINLGYTTIYAPVSGQVGQANYREGALIAPTEDQLATLSVIDPIWINFSISEGDILRSQKSI
SQGKLHFPAYDNFTVQVILADQTVFPEIGKVNFSSPTYDQKTGTMMVRAVLPNPKNILRPGQFVRVQLLGATRPNAILVP
QKAVLQSQKGMFVFLVNKDDEVVMQPIEPGVWDQQNWIIDAGLKAGDQVVVDGVNKLRPGMKVSIKKKIFAEKAS
>Mature_395_residues
MFDSKEKSLREILMPKKNCFFYYFCFFLASLCLSSCEKEKTTNFQAPSTDVSIQIVEPKTVPAVFEFVGIAQSSHVVEIR
ARIEGYLDKIAYTEGSFVQKGNLLFQIDPRPFEAALEKATAQLAREEAVLWDAKRAVDRYKPLYEKKAASLRDLDNAIAR
QLSSEADVQAAKAQLKEADINLGYTTIYAPVSGQVGQANYREGALIAPTEDQLATLSVIDPIWINFSISEGDILRSQKSI
SQGKLHFPAYDNFTVQVILADQTVFPEIGKVNFSSPTYDQKTGTMMVRAVLPNPKNILRPGQFVRVQLLGATRPNAILVP
QKAVLQSQKGMFVFLVNKDDEVVMQPIEPGVWDQQNWIIDAGLKAGDQVVVDGVNKLRPGMKVSIKKKIFAEKAS

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=387, Percent_Identity=35.4005167958656, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1789929, Length=375, Percent_Identity=31.4666666666667, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1786668, Length=374, Percent_Identity=35.2941176470588, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87082036, Length=329, Percent_Identity=31.6109422492401, Blast_Score=163, Evalue=2e-41,
Organism=Escherichia coli, GI87081796, Length=357, Percent_Identity=25.7703081232493, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI87081951, Length=127, Percent_Identity=34.6456692913386, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 43994; Mature: 43994

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDSKEKSLREILMPKKNCFFYYFCFFLASLCLSSCEKEKTTNFQAPSTDVSIQIVEPKT
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
VPAVFEFVGIAQSSHVVEIRARIEGYLDKIAYTEGSFVQKGNLLFQIDPRPFEAALEKAT
HHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCHHHHHHHHHH
AQLAREEAVLWDAKRAVDRYKPLYEKKAASLRDLDNAIARQLSSEADVQAAKAQLKEADI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
NLGYTTIYAPVSGQVGQANYREGALIAPTEDQLATLSVIDPIWINFSISEGDILRSQKSI
CCCEEEEEECCCCCCCCCCCCCCCEECCCCHHEEEEEEECEEEEEEEECCCHHHHHHHHH
SQGKLHFPAYDNFTVQVILADQTVFPEIGKVNFSSPTYDQKTGTMMVRAVLPNPKNILRP
CCCEEECCCCCCEEEEEEEECCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCHHHCCC
GQFVRVQLLGATRPNAILVPQKAVLQSQKGMFVFLVNKDDEVVMQPIEPGVWDQQNWIID
CCEEEEEEEECCCCCEEECCCHHHHHCCCCEEEEEECCCCCEEEECCCCCCCCCCCEEEE
AGLKAGDQVVVDGVNKLRPGMKVSIKKKIFAEKAS
CCCCCCCEEEECCHHHHCCCCEEEEHHHHHHCCCC
>Mature Secondary Structure
MFDSKEKSLREILMPKKNCFFYYFCFFLASLCLSSCEKEKTTNFQAPSTDVSIQIVEPKT
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCC
VPAVFEFVGIAQSSHVVEIRARIEGYLDKIAYTEGSFVQKGNLLFQIDPRPFEAALEKAT
HHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHCCCCCEEECCCEEEEECCCHHHHHHHHHH
AQLAREEAVLWDAKRAVDRYKPLYEKKAASLRDLDNAIARQLSSEADVQAAKAQLKEADI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
NLGYTTIYAPVSGQVGQANYREGALIAPTEDQLATLSVIDPIWINFSISEGDILRSQKSI
CCCEEEEEECCCCCCCCCCCCCCCEECCCCHHEEEEEEECEEEEEEEECCCHHHHHHHHH
SQGKLHFPAYDNFTVQVILADQTVFPEIGKVNFSSPTYDQKTGTMMVRAVLPNPKNILRP
CCCEEECCCCCCEEEEEEEECCCCCCCCCCEECCCCCCCCCCCEEEEEEECCCCHHHCCC
GQFVRVQLLGATRPNAILVPQKAVLQSQKGMFVFLVNKDDEVVMQPIEPGVWDQQNWIID
CCEEEEEEEECCCCCEEECCCHHHHHCCCCEEEEEECCCCCEEEECCCCCCCCCCCEEEE
AGLKAGDQVVVDGVNKLRPGMKVSIKKKIFAEKAS
CCCCCCCEEEECCHHHHCCCCEEEEHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]