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The map label for this gene is rf-2 [H]

Identifier: 46446182

GI number: 46446182

Start: 680787

End: 681143

Strand: Reverse

Name: rf-2 [H]

Synonym: pc0548

Alternate gene names: 46446182

Gene position: 681143-680787 (Counterclockwise)

Preceding gene: 46446187

Following gene: 46446181

Centisome position: 28.21

GC content: 35.29

Gene sequence:

>357_bases
ATGAGTAAACAAAAAATTATTTTACCTGAACAAGATGAAGATTTACTAGCTGAGTGCCAAGTTCAAACTTTTCGCTCAAG
TGGAAGTGGAGGACAACATATTAATGTTACCAACAGCGCTGTCCGTTTGACCCATTTTCCGACAGGAATTGTTGTATCTA
GTCAAACTTTTCGCAGTCAATATCGAAATAAGCAAAATTGTCTCGCTAAATTAAGACAGACCGTGGAGAAACTGAATTAT
CGTCAACCTAAACGCATTCCAACTAAAATTCCTAGAACAGTTAAAAACAAGAATGTGGTAAAAAAAGAACGCCATTCTCA
AAAAAAAATCCTACGTAAACCTCCTAAATTTGACTAA

Upstream 100 bases:

>100_bases
CCAAAAAAAACTAATATCATTTTTCCCTGAATTTTGATTCATTTATATTGCATATCTTTATAACTTTAGTTATTTATATT
CAAAGAACTAAAAATTAATT

Downstream 100 bases:

>100_bases
ATGATAATAGACAAATGAAATTGATTAAATTTCCCTTCACTTACTTATCCTCTCTTCTTACCATTTAAGGGGGTATTTTA
TTAGGTGGGTATGATGGTGT

Product: putative peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 118; Mature: 117

Protein sequence:

>118_residues
MSKQKIILPEQDEDLLAECQVQTFRSSGSGGQHINVTNSAVRLTHFPTGIVVSSQTFRSQYRNKQNCLAKLRQTVEKLNY
RQPKRIPTKIPRTVKNKNVVKKERHSQKKILRKPPKFD

Sequences:

>Translated_118_residues
MSKQKIILPEQDEDLLAECQVQTFRSSGSGGQHINVTNSAVRLTHFPTGIVVSSQTFRSQYRNKQNCLAKLRQTVEKLNY
RQPKRIPTKIPRTVKNKNVVKKERHSQKKILRKPPKFD
>Mature_117_residues
SKQKIILPEQDEDLLAECQVQTFRSSGSGGQHINVTNSAVRLTHFPTGIVVSSQTFRSQYRNKQNCLAKLRQTVEKLNYR
QPKRIPTKIPRTVKNKNVVKKERHSQKKILRKPPKFD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=74, Percent_Identity=44.5945945945946, Blast_Score=68, Evalue=2e-12,
Organism=Homo sapiens, GI34577120, Length=81, Percent_Identity=45.679012345679, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787462, Length=55, Percent_Identity=58.1818181818182, Blast_Score=70, Evalue=4e-14,
Organism=Escherichia coli, GI2367172, Length=81, Percent_Identity=39.5061728395062, Blast_Score=70, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6321295, Length=56, Percent_Identity=57.1428571428571, Blast_Score=70, Evalue=5e-14,
Organism=Drosophila melanogaster, GI19921226, Length=74, Percent_Identity=39.1891891891892, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 13669; Mature: 13538

Theoretical pI: Translated: 11.32; Mature: 11.32

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQKIILPEQDEDLLAECQVQTFRSSGSGGQHINVTNSAVRLTHFPTGIVVSSQTFRSQ
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCEEECCHHHHHH
YRNKQNCLAKLRQTVEKLNYRQPKRIPTKIPRTVKNKNVVKKERHSQKKILRKPPKFD
HCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SKQKIILPEQDEDLLAECQVQTFRSSGSGGQHINVTNSAVRLTHFPTGIVVSSQTFRSQ
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEEECCCCEEECCHHHHHH
YRNKQNCLAKLRQTVEKLNYRQPKRIPTKIPRTVKNKNVVKKERHSQKKILRKPPKFD
HCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10684935 [H]