Click here to switch to the map view.

The map label for this gene is folE

Identifier: 45659222

GI number: 45659222

Start: 4175598

End: 4176149

Strand: Reverse

Name: folE

Synonym: LIC13405

Alternate gene names: 45659222

Gene position: 4176149-4175598 (Counterclockwise)

Preceding gene: 45659224

Following gene: 45659221

Centisome position: 97.64

GC content: 37.5

Gene sequence:

>552_bases
TTGGAAGAAGATGTAATTAATATTTTAAAATCGATTGGAGAAGATCCAACTAGAGAAGGCCTTCTCAATACTCCAAAACG
AGTTAAAAAGGCGTACGAATTTTTGACATCCGGTTATCGCGCTGACATTACAAAAATTGTAAATGGAGCCATCTTTGAAG
AACCAACAGAAGGAATGGTTTTAGTTAGAGATATAGAAATGTATTCCCTTTGTGAACATCACCTTCTTCCTTTTTATGGA
AAGGCGCACGTGGCTTATCTCCCAAACAAAAAGATCATAGGGATTAGTAAAATTCCTAGAATTGTGGATGTTTTTGCCAG
AAGGTTGCAAGTTCAAGAACGTCTCACAGAACAGATTGCTTACGCTATTCAAGAAGTTTTAGATCCTCAAGGTGTTGCCG
TGGTAATCAAGGCAAAACATCTTTGTATGATGATGAGAGGGGTGGAAAAACAAAATTCTGAACTTTTCACCTCGTGTATG
TTAGGCGCTTTTAAAGAAAACATGGTCACTCGTTCTGAATTTTTGGATCTGATTCGTACTGGATCCACTTAA

Upstream 100 bases:

>100_bases
TAGCCACTTGGATTTTGTGGATGTATTGAAGTATAAATCCCTATAAGTTTTTAGAAAAACTTAATGATTTTTAAAAGAAA
ATTGAACAAGAGAGTTTAAA

Downstream 100 bases:

>100_bases
AGGATAGTTCTTAATCATAAATTTTATAAAAAACTTCTTTCGCAATTTGGTAGACAGAGAGAAAAAAAGAAACATTCTTT
GCCGAATTCGGAGGAAGTTA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I; GTP-CH-I [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MEEDVINILKSIGEDPTREGLLNTPKRVKKAYEFLTSGYRADITKIVNGAIFEEPTEGMVLVRDIEMYSLCEHHLLPFYG
KAHVAYLPNKKIIGISKIPRIVDVFARRLQVQERLTEQIAYAIQEVLDPQGVAVVIKAKHLCMMMRGVEKQNSELFTSCM
LGAFKENMVTRSEFLDLIRTGST

Sequences:

>Translated_183_residues
MEEDVINILKSIGEDPTREGLLNTPKRVKKAYEFLTSGYRADITKIVNGAIFEEPTEGMVLVRDIEMYSLCEHHLLPFYG
KAHVAYLPNKKIIGISKIPRIVDVFARRLQVQERLTEQIAYAIQEVLDPQGVAVVIKAKHLCMMMRGVEKQNSELFTSCM
LGAFKENMVTRSEFLDLIRTGST
>Mature_183_residues
MEEDVINILKSIGEDPTREGLLNTPKRVKKAYEFLTSGYRADITKIVNGAIFEEPTEGMVLVRDIEMYSLCEHHLLPFYG
KAHVAYLPNKKIIGISKIPRIVDVFARRLQVQERLTEQIAYAIQEVLDPQGVAVVIKAKHLCMMMRGVEKQNSELFTSCM
LGAFKENMVTRSEFLDLIRTGST

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=174, Percent_Identity=57.4712643678161, Blast_Score=224, Evalue=3e-59,
Organism=Homo sapiens, GI4503949, Length=174, Percent_Identity=57.4712643678161, Blast_Score=224, Evalue=3e-59,
Organism=Homo sapiens, GI66932970, Length=155, Percent_Identity=52.258064516129, Blast_Score=175, Evalue=2e-44,
Organism=Homo sapiens, GI66932972, Length=132, Percent_Identity=56.8181818181818, Blast_Score=170, Evalue=5e-43,
Organism=Escherichia coli, GI1788476, Length=177, Percent_Identity=42.3728813559322, Blast_Score=121, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17560486, Length=172, Percent_Identity=55.8139534883721, Blast_Score=216, Evalue=5e-57,
Organism=Saccharomyces cerevisiae, GI6321706, Length=173, Percent_Identity=55.4913294797688, Blast_Score=209, Evalue=2e-55,
Organism=Drosophila melanogaster, GI24656782, Length=173, Percent_Identity=53.1791907514451, Blast_Score=200, Evalue=5e-52,
Organism=Drosophila melanogaster, GI24656791, Length=173, Percent_Identity=53.1791907514451, Blast_Score=199, Evalue=1e-51,
Organism=Drosophila melanogaster, GI24656796, Length=173, Percent_Identity=53.1791907514451, Blast_Score=199, Evalue=1e-51,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 20792; Mature: 20792

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEDVINILKSIGEDPTREGLLNTPKRVKKAYEFLTSGYRADITKIVNGAIFEEPTEGMV
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEE
LVRDIEMYSLCEHHLLPFYGKAHVAYLPNKKIIGISKIPRIVDVFARRLQVQERLTEQIA
EEEHHHHHHHHHHHCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHH
YAIQEVLDPQGVAVVIKAKHLCMMMRGVEKQNSELFTSCMLGAFKENMVTRSEFLDLIRT
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GST
CCC
>Mature Secondary Structure
MEEDVINILKSIGEDPTREGLLNTPKRVKKAYEFLTSGYRADITKIVNGAIFEEPTEGMV
CCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCEE
LVRDIEMYSLCEHHLLPFYGKAHVAYLPNKKIIGISKIPRIVDVFARRLQVQERLTEQIA
EEEHHHHHHHHHHHCCCCCCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHH
YAIQEVLDPQGVAVVIKAKHLCMMMRGVEKQNSELFTSCMLGAFKENMVTRSEFLDLIRT
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GST
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA