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The map label for this gene is cysH

Identifier: 45659191

GI number: 45659191

Start: 4134872

End: 4135612

Strand: Reverse

Name: cysH

Synonym: LIC13372

Alternate gene names: 45659191

Gene position: 4135612-4134872 (Counterclockwise)

Preceding gene: 45659192

Following gene: 45659190

Centisome position: 96.69

GC content: 40.49

Gene sequence:

>741_bases
ATGAACGCGCAAGAGTTGGAACAAAAACTTGCTCCTTTGAAGAACCTAGAAGACGCCTTAGAATGGATTAGTAAAGAATA
TGGAGAAACTGCGGCTTTCTCTACAAGCTTGGGTCTTGAAGATCAAGTCATCACTCACGTTATATTCTCTCGAAATCTTA
AAATTAGAATTTTTACTTTGGATACGGGGCGGCTTTTCAACGAGACCTACGATTTACATAAATTGACAAACGCAAGTTAT
GGTAAAAAGATAGAAACTTATTTTCCCGACACGAATGCGGTTCAAAACCTAATCAACTTAAAAGGTCCCGACAGTTTCTA
CGACTCAGTTGAAAACAGAAAAGAATGTTGTTATATTCGAAAAGTCGAACCACTTAATCGTGCGTTAGTCGGAACCAAGC
TTTGGATCACCGGAATCCGTTCCGAACAATCCGATTCTAGAAATTCTCTCACAAAAGTAGAACTGGATTCTTCTCGAAAC
ATACTCAAGTATCATCCTCTTTTGGATTGGTCCTTGGAACGCATTCAAGATTTTATAGATACGTATCGGATTCCGACTAA
CGTTCTTCATAAAAAAGGTTTTCCTTCTATCGGGTGTGCTCCCTGCACAAGAGCAATTCAACCCGGAGAAGATATTCGCG
CCGGAAGATGGTGGTGGGAAGAATCCAACCAAGAATGTGGACTTCACGTTGTAGACGGCAAGTTAGTTCGTCAAAAGCTT
GGTCCTAAAAGAGCAATATGA

Upstream 100 bases:

>100_bases
AATAACTCCATTTTTTCTTAAATCTGGATATTTCTTGAATCAATTATAAAATCAAGCTGGATGCGTAGATCTACCATTCA
AGACTTAGGTTATCACAAAC

Downstream 100 bases:

>100_bases
CTTCAACCATGAATAGATCTAGACTTACACATCTTGAACAACTAGAAGCAGAATCGATCTATATTCTTAGAGAAACCGCT
TCTCAGTTTGAAAGACCTGC

Product: phosphoadenosine phosphosulphate reductase

Products: NA

Alternate protein names: 3'-phosphoadenylylsulfate reductase; PAPS reductase, thioredoxin dependent; PAPS sulfotransferase; PAdoPS reductase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MNAQELEQKLAPLKNLEDALEWISKEYGETAAFSTSLGLEDQVITHVIFSRNLKIRIFTLDTGRLFNETYDLHKLTNASY
GKKIETYFPDTNAVQNLINLKGPDSFYDSVENRKECCYIRKVEPLNRALVGTKLWITGIRSEQSDSRNSLTKVELDSSRN
ILKYHPLLDWSLERIQDFIDTYRIPTNVLHKKGFPSIGCAPCTRAIQPGEDIRAGRWWWEESNQECGLHVVDGKLVRQKL
GPKRAI

Sequences:

>Translated_246_residues
MNAQELEQKLAPLKNLEDALEWISKEYGETAAFSTSLGLEDQVITHVIFSRNLKIRIFTLDTGRLFNETYDLHKLTNASY
GKKIETYFPDTNAVQNLINLKGPDSFYDSVENRKECCYIRKVEPLNRALVGTKLWITGIRSEQSDSRNSLTKVELDSSRN
ILKYHPLLDWSLERIQDFIDTYRIPTNVLHKKGFPSIGCAPCTRAIQPGEDIRAGRWWWEESNQECGLHVVDGKLVRQKL
GPKRAI
>Mature_246_residues
MNAQELEQKLAPLKNLEDALEWISKEYGETAAFSTSLGLEDQVITHVIFSRNLKIRIFTLDTGRLFNETYDLHKLTNASY
GKKIETYFPDTNAVQNLINLKGPDSFYDSVENRKECCYIRKVEPLNRALVGTKLWITGIRSEQSDSRNSLTKVELDSSRN
ILKYHPLLDWSLERIQDFIDTYRIPTNVLHKKGFPSIGCAPCTRAIQPGEDIRAGRWWWEESNQECGLHVVDGKLVRQKL
GPKRAI

Specific function: Reduction of activated sulfate into sulfite [H]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysH subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789121, Length=213, Percent_Identity=28.169014084507, Blast_Score=77, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325425, Length=205, Percent_Identity=29.7560975609756, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011798
- InterPro:   IPR004511
- InterPro:   IPR002500
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01507 PAPS_reduct [H]

EC number: =1.8.4.8 [H]

Molecular weight: Translated: 28287; Mature: 28287

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAQELEQKLAPLKNLEDALEWISKEYGETAAFSTSLGLEDQVITHVIFSRNLKIRIFTL
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHEECCCCCCHHHHHHHHHHCCCEEEEEEE
DTGRLFNETYDLHKLTNASYGKKIETYFPDTNAVQNLINLKGPDSFYDSVENRKECCYIR
CCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHCHHHEEEEE
KVEPLNRALVGTKLWITGIRSEQSDSRNSLTKVELDSSRNILKYHPLLDWSLERIQDFID
CCCHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHH
TYRIPTNVLHKKGFPSIGCAPCTRAIQPGEDIRAGRWWWEESNQECGLHVVDGKLVRQKL
HHCCCHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCEECCCCCCCCEEEECHHHHHHHC
GPKRAI
CCCCCC
>Mature Secondary Structure
MNAQELEQKLAPLKNLEDALEWISKEYGETAAFSTSLGLEDQVITHVIFSRNLKIRIFTL
CCHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHEECCCCCCHHHHHHHHHHCCCEEEEEEE
DTGRLFNETYDLHKLTNASYGKKIETYFPDTNAVQNLINLKGPDSFYDSVENRKECCYIR
CCCHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHCHHHEEEEE
KVEPLNRALVGTKLWITGIRSEQSDSRNSLTKVELDSSRNILKYHPLLDWSLERIQDFID
CCCHHHHHHHHHHHEEEECCCCCCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHH
TYRIPTNVLHKKGFPSIGCAPCTRAIQPGEDIRAGRWWWEESNQECGLHVVDGKLVRQKL
HHCCCHHHHHHCCCCCCCCHHHHHCCCCCCCCCCCCCEECCCCCCCCEEEECHHHHHHHC
GPKRAI
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10464198; 11481430 [H]