Click here to switch to the map view.

The map label for this gene is mraW

Identifier: 45657732

GI number: 45657732

Start: 2264363

End: 2265367

Strand: Reverse

Name: mraW

Synonym: LIC11869

Alternate gene names: 45657732

Gene position: 2265367-2264363 (Counterclockwise)

Preceding gene: 45657734

Following gene: 45657731

Centisome position: 52.96

GC content: 35.82

Gene sequence:

>1005_bases
ATGGAATCTCCTTGCAGGACCATCACTTTAGAAAAGAATGGAACTAGTTTGGAACCAGTTCATTATTCAGTTCAAGGAAA
AGAGATTCTTCAAATTTTTAAGGAGAATTTTCAAAAAGAAGATCCGGTATTATTTTTAGATGGAACTGCTGGAGAAGGAG
GGCATAGCTTTTTATTTTTAAAAGAATTTCCCAATTCCAGAATTATACTTTGTGACAGAGATCCAATTATGCTTTCAAGG
GCGCTCACAAGGCTTTCCGATTTTTCAGAAAGGGTCGTTTCGATCCAAACAAACTTTTCCGAGATCAATCAAAAGCTTCT
AACTTCTTATGGAATTGATCAAACTCCACAGGGAATTTTACTGGATCTTGGAATTTCCACATTTCATCTTTTTCATTCAG
GGAGGGGTTTTAGTTTTCGAGAATCAGAACCTTTGGATATGAGACTCAACCCAAATAGTGGACAGAGTGCAGAAGAAATT
CTGAATATATATCCTAAAGATAGATTGATGAATATATTTTATACGTATGGAGAAGAGAGATGGTCCAAAAAAATCGCAGA
AGTGATTGTAGAAACTAGAAAACAAAATTCAATTTCAACTACTTTTGAACTTGCGAATTTGGTTTCTAAAATAATTCCCC
GAAAGTTTTGGCCGCCGGGAAGACATCCAGCAACTCGAATCTTTCAAGCGCTTCGGATTGAAGTCAATCAGGAGTTGGCT
CATATCGAAAATGGACTCAAACTCCTTTTGGATCTTTTGCGTTTGGGAGGAGTGATACAAGTGATCTCGTTTCATTCTCT
AGAAGATAGAATCGTTAAAAATTCTTTTAGAGATTATGCGAAACAAAACGGATTTGAGCTTCTTACTAAAAAGCCGATTC
TTCCTTCCCAAGAGGAAATTGACGAGAATCCCGCTTCTCGTTCAGCAAAATTGAGAATACTTAGAAAAACAAAATCGGTC
GATAAAAAATATAGAAATAAAAATTTCGAAGAAGAGGAAGAATAA

Upstream 100 bases:

>100_bases
CCCTGAAAATGTGGGAACCCACACAAAATAACCGATTCGATTCAATTTTTGAGAATTTCTACAGATGGGCCGTCAAGAAT
TTTCATGATTTAGAGTTATA

Downstream 100 bases:

>100_bases
AAATAGATGTCGTCGTTTTCTGCGTTACTAAAACATTCCGTTTGGAGAGATCTCTGGACTTTATTTTTGAACCTAGGAAA
AGTGATTCTTTTTTTAAGTT

Product: S-adenosyl-methyltransferase

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MESPCRTITLEKNGTSLEPVHYSVQGKEILQIFKENFQKEDPVLFLDGTAGEGGHSFLFLKEFPNSRIILCDRDPIMLSR
ALTRLSDFSERVVSIQTNFSEINQKLLTSYGIDQTPQGILLDLGISTFHLFHSGRGFSFRESEPLDMRLNPNSGQSAEEI
LNIYPKDRLMNIFYTYGEERWSKKIAEVIVETRKQNSISTTFELANLVSKIIPRKFWPPGRHPATRIFQALRIEVNQELA
HIENGLKLLLDLLRLGGVIQVISFHSLEDRIVKNSFRDYAKQNGFELLTKKPILPSQEEIDENPASRSAKLRILRKTKSV
DKKYRNKNFEEEEE

Sequences:

>Translated_334_residues
MESPCRTITLEKNGTSLEPVHYSVQGKEILQIFKENFQKEDPVLFLDGTAGEGGHSFLFLKEFPNSRIILCDRDPIMLSR
ALTRLSDFSERVVSIQTNFSEINQKLLTSYGIDQTPQGILLDLGISTFHLFHSGRGFSFRESEPLDMRLNPNSGQSAEEI
LNIYPKDRLMNIFYTYGEERWSKKIAEVIVETRKQNSISTTFELANLVSKIIPRKFWPPGRHPATRIFQALRIEVNQELA
HIENGLKLLLDLLRLGGVIQVISFHSLEDRIVKNSFRDYAKQNGFELLTKKPILPSQEEIDENPASRSAKLRILRKTKSV
DKKYRNKNFEEEEE
>Mature_334_residues
MESPCRTITLEKNGTSLEPVHYSVQGKEILQIFKENFQKEDPVLFLDGTAGEGGHSFLFLKEFPNSRIILCDRDPIMLSR
ALTRLSDFSERVVSIQTNFSEINQKLLTSYGIDQTPQGILLDLGISTFHLFHSGRGFSFRESEPLDMRLNPNSGQSAEEI
LNIYPKDRLMNIFYTYGEERWSKKIAEVIVETRKQNSISTTFELANLVSKIIPRKFWPPGRHPATRIFQALRIEVNQELA
HIENGLKLLLDLLRLGGVIQVISFHSLEDRIVKNSFRDYAKQNGFELLTKKPILPSQEEIDENPASRSAKLRILRKTKSV
DKKYRNKNFEEEEE

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=312, Percent_Identity=33.0128205128205, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI165377202, Length=170, Percent_Identity=32.9411764705882, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1786270, Length=296, Percent_Identity=40.2027027027027, Blast_Score=186, Evalue=1e-48,
Organism=Drosophila melanogaster, GI62472493, Length=311, Percent_Identity=34.0836012861736, Blast_Score=144, Evalue=6e-35,
Organism=Drosophila melanogaster, GI28571637, Length=311, Percent_Identity=34.0836012861736, Blast_Score=144, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_LEPIC (P62472)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_001818.1
- ProteinModelPortal:   P62472
- SMR:   P62472
- GeneID:   2771393
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC11869
- NMPDR:   fig|267671.1.peg.1818
- HOGENOM:   HBG302779
- OMA:   HEAHREG
- ProtClustDB:   CLSK574166
- BioCyc:   LINT-130-01:LINT-130-01-001818-MONOMER
- BioCyc:   LINT267671:LIC_11869-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 38474; Mature: 38474

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: NA

Important sites: BINDING 72-72 BINDING 99-99 BINDING 122-122 BINDING 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESPCRTITLEKNGTSLEPVHYSVQGKEILQIFKENFQKEDPVLFLDGTAGEGGHSFLFL
CCCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEE
KEFPNSRIILCDRDPIMLSRALTRLSDFSERVVSIQTNFSEINQKLLTSYGIDQTPQGIL
EECCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHCCCCCCCCCEE
LDLGISTFHLFHSGRGFSFRESEPLDMRLNPNSGQSAEEILNIYPKDRLMNIFYTYGEER
EECCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHH
WSKKIAEVIVETRKQNSISTTFELANLVSKIIPRKFWPPGRHPATRIFQALRIEVNQELA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HIENGLKLLLDLLRLGGVIQVISFHSLEDRIVKNSFRDYAKQNGFELLTKKPILPSQEEI
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHH
DENPASRSAKLRILRKTKSVDKKYRNKNFEEEEE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MESPCRTITLEKNGTSLEPVHYSVQGKEILQIFKENFQKEDPVLFLDGTAGEGGHSFLFL
CCCCCCEEEEECCCCCCCCEEEECCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEE
KEFPNSRIILCDRDPIMLSRALTRLSDFSERVVSIQTNFSEINQKLLTSYGIDQTPQGIL
EECCCCEEEEECCCHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHCCCCCCCCCEE
LDLGISTFHLFHSGRGFSFRESEPLDMRLNPNSGQSAEEILNIYPKDRLMNIFYTYGEER
EECCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHH
WSKKIAEVIVETRKQNSISTTFELANLVSKIIPRKFWPPGRHPATRIFQALRIEVNQELA
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHH
HIENGLKLLLDLLRLGGVIQVISFHSLEDRIVKNSFRDYAKQNGFELLTKKPILPSQEEI
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHH
DENPASRSAKLRILRKTKSVDKKYRNKNFEEEEE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA