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The map label for this gene is murE

Identifier: 45657730

GI number: 45657730

Start: 2262505

End: 2264025

Strand: Reverse

Name: murE

Synonym: LIC11867

Alternate gene names: 45657730

Gene position: 2264025-2262505 (Counterclockwise)

Preceding gene: 45657731

Following gene: 45657729

Centisome position: 52.93

GC content: 37.21

Gene sequence:

>1521_bases
ATGAAAATGAAGTTAACCAGTCTTCTCCTTAAATTTCCAGAAATCAAACTAAAGTCTTTTCCTTCCGGAAAAAATCCGGA
TTCAATCGAAATCGAATATATTCAATCGGATTCTAGAAAAACAAACAAAAACGATATATTCTGTGTAGCCGATTCTATTG
GTTCCAAAAAGAAAGAATTTATTTCCAACGCAAAAGCTTCCCTAATTTTACTACGAACCGATTCAAATGTTTTGAATGAT
CTTTTAGAAGTTATGAATTCTTCCAAAATTTTTTTGGAATGTGAAATAGATCCGGAGCAGCTACAAGGTAGAATCGCATC
TTTTCTTTTAGGACATCCTTCTAAAGATTTGGATATTGTAGCCGTAACCGGGACAAACGGTAAAACTTCTTTGACGAATA
TTCTATTCTCATTAGCAAAAGACCAAGGAATCAATTGTGGATTGATCGGAACAATTGGAGTTAAGTTTGGAGATAGAGTG
ATAGACACAGGTTATACTACACCGGATGCTTCTTCTCTCAACTTAATACTCAAAGAAATGAAAGAAGAAGGAATCACTAC
CGTTTTTATGGAGGCAAGTTCTCACGGTCTCAAATTAGGTAGAATGAACGGGATTTCTGTAAGGGCAGGAGTATTTACAA
ATCTTACTCAAGATCACCTAGATTTTCATTCCGATATGGAGGATTATTTTGAAAGTAAATTTCGTCTTTTTGAAATTTTA
GATTTTTCTAAATCTACTTTTGCGGTTTTAGACTATTCTGCGCCAAATGGCAGCAAACTCTATCATAAAATTCTAAATCG
ATTTCCTGATCTTTTAATATATGCGTTAGACGATATATATAGAAAATGGAAGATAAGTGACATTTCTTTAAACTTACAAG
GTACTTCCTACGTTTTAGGCTTACCTGGAAACCAGGAACGAAAGATTTCAACCAATCTGCTCGGTTCATTTAACGTTCGA
AATACGGCTCTTGCGTTTTTGACCGGAATTGGTATAGGTTTAGATCTGGAAAAGATGTCTAACTCTCTGGAAAAAATTCC
TCAGATTCCTGGAAGATTTCAGATCATCTACAGCAAAGATCGTTCCAGGATGGCGGTCGTGGATTACGCACACACTCCGG
ATGCTCTCGAAAATATAATTCGAAGTGTAAGAGATTCTCAACCTAAATGTTTGATTACTTTGTTCGGATGTGGAGGAGAT
CGGGACCGAACCAAACGTCCTAAAATGGCTCGAATCGCCGAAGAACTTTCCGATCAGGTGATCTTGACCTCCGATAATCC
TAGAACGGAAAAACCAGAAACGATTTTAGATGAAATTCAAACCGGGTTTTCCTCCGGTTTTATTCCACTTTTACGGGAAG
TAGACCGTGCGAAAGCAATTGTAGAAGGTATTTCTTGTTTACCCGAAGGAGGGTGTTTACTTGTGGCTGGTAAAGGACAC
GAAGAATACCAAATCATTGGAAAGGAAAAACGTCATTTCAGTGACGTGGAAGAAGTTCAAAAAGCGTTCGGGCTTTTTTA
G

Upstream 100 bases:

>100_bases
AGAAGAATCGAAACTCTTTACAGAAAAACATACAACTATCTTCCTATCACAGTGGGGGATAGAATCGTCACTTTGAATTT
ACCTCCTGAAAAAAACGAAG

Downstream 100 bases:

>100_bases
AAATTTTCCGATACAAAAAAAGAATACTCCGTTTTTTTGAAAACAGGGCAGGAAAAATGTTTTATTATCTTTATGATCTT
TATTTCAATCATCTAGACTC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKMKLTSLLLKFPEIKLKSFPSGKNPDSIEIEYIQSDSRKTNKNDIFCVADSIGSKKKEFISNAKASLILLRTDSNVLND
LLEVMNSSKIFLECEIDPEQLQGRIASFLLGHPSKDLDIVAVTGTNGKTSLTNILFSLAKDQGINCGLIGTIGVKFGDRV
IDTGYTTPDASSLNLILKEMKEEGITTVFMEASSHGLKLGRMNGISVRAGVFTNLTQDHLDFHSDMEDYFESKFRLFEIL
DFSKSTFAVLDYSAPNGSKLYHKILNRFPDLLIYALDDIYRKWKISDISLNLQGTSYVLGLPGNQERKISTNLLGSFNVR
NTALAFLTGIGIGLDLEKMSNSLEKIPQIPGRFQIIYSKDRSRMAVVDYAHTPDALENIIRSVRDSQPKCLITLFGCGGD
RDRTKRPKMARIAEELSDQVILTSDNPRTEKPETILDEIQTGFSSGFIPLLREVDRAKAIVEGISCLPEGGCLLVAGKGH
EEYQIIGKEKRHFSDVEEVQKAFGLF

Sequences:

>Translated_506_residues
MKMKLTSLLLKFPEIKLKSFPSGKNPDSIEIEYIQSDSRKTNKNDIFCVADSIGSKKKEFISNAKASLILLRTDSNVLND
LLEVMNSSKIFLECEIDPEQLQGRIASFLLGHPSKDLDIVAVTGTNGKTSLTNILFSLAKDQGINCGLIGTIGVKFGDRV
IDTGYTTPDASSLNLILKEMKEEGITTVFMEASSHGLKLGRMNGISVRAGVFTNLTQDHLDFHSDMEDYFESKFRLFEIL
DFSKSTFAVLDYSAPNGSKLYHKILNRFPDLLIYALDDIYRKWKISDISLNLQGTSYVLGLPGNQERKISTNLLGSFNVR
NTALAFLTGIGIGLDLEKMSNSLEKIPQIPGRFQIIYSKDRSRMAVVDYAHTPDALENIIRSVRDSQPKCLITLFGCGGD
RDRTKRPKMARIAEELSDQVILTSDNPRTEKPETILDEIQTGFSSGFIPLLREVDRAKAIVEGISCLPEGGCLLVAGKGH
EEYQIIGKEKRHFSDVEEVQKAFGLF
>Mature_506_residues
MKMKLTSLLLKFPEIKLKSFPSGKNPDSIEIEYIQSDSRKTNKNDIFCVADSIGSKKKEFISNAKASLILLRTDSNVLND
LLEVMNSSKIFLECEIDPEQLQGRIASFLLGHPSKDLDIVAVTGTNGKTSLTNILFSLAKDQGINCGLIGTIGVKFGDRV
IDTGYTTPDASSLNLILKEMKEEGITTVFMEASSHGLKLGRMNGISVRAGVFTNLTQDHLDFHSDMEDYFESKFRLFEIL
DFSKSTFAVLDYSAPNGSKLYHKILNRFPDLLIYALDDIYRKWKISDISLNLQGTSYVLGLPGNQERKISTNLLGSFNVR
NTALAFLTGIGIGLDLEKMSNSLEKIPQIPGRFQIIYSKDRSRMAVVDYAHTPDALENIIRSVRDSQPKCLITLFGCGGD
RDRTKRPKMARIAEELSDQVILTSDNPRTEKPETILDEIQTGFSSGFIPLLREVDRAKAIVEGISCLPEGGCLLVAGKGH
EEYQIIGKEKRHFSDVEEVQKAFGLF

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily

Homologues:

Organism=Escherichia coli, GI1786273, Length=484, Percent_Identity=31.6115702479339, Blast_Score=210, Evalue=2e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURE_LEPIC (Q72R81)

Other databases:

- EMBL:   AE016823
- RefSeq:   YP_001816.1
- ProteinModelPortal:   Q72R81
- GeneID:   2770780
- GenomeReviews:   AE016823_GR
- KEGG:   lic:LIC11867
- HOGENOM:   HBG602753
- OMA:   GHEEGQT
- ProtClustDB:   CLSK574168
- BioCyc:   LINT267671:LIC_11867-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00208
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- TIGRFAMs:   TIGR01085

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.13

Molecular weight: Translated: 56318; Mature: 56318

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: BINDING 38-38 BINDING 193-193 BINDING 201-201 BINDING 401-401 BINDING 477-477 BINDING 481-481

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMKLTSLLLKFPEIKLKSFPSGKNPDSIEIEYIQSDSRKTNKNDIFCVADSIGSKKKEF
CCCHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCHHHHH
ISNAKASLILLRTDSNVLNDLLEVMNSSKIFLECEIDPEQLQGRIASFLLGHPSKDLDIV
HCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEE
AVTGTNGKTSLTNILFSLAKDQGINCGLIGTIGVKFGDRVIDTGYTTPDASSLNLILKEM
EEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHH
KEEGITTVFMEASSHGLKLGRMNGISVRAGVFTNLTQDHLDFHSDMEDYFESKFRLFEIL
HHCCCEEEEEECCCCCEEEECCCCCEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
DFSKSTFAVLDYSAPNGSKLYHKILNRFPDLLIYALDDIYRKWKISDISLNLQGTSYVLG
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCEEEEE
LPGNQERKISTNLLGSFNVRNTALAFLTGIGIGLDLEKMSNSLEKIPQIPGRFQIIYSKD
CCCCCCCEEHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECC
RSRMAVVDYAHTPDALENIIRSVRDSQPKCLITLFGCGGDRDRTKRPKMARIAEELSDQV
CCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCE
ILTSDNPRTEKPETILDEIQTGFSSGFIPLLREVDRAKAIVEGISCLPEGGCLLVAGKGH
EEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
EEYQIIGKEKRHFSDVEEVQKAFGLF
CCEEEECCHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MKMKLTSLLLKFPEIKLKSFPSGKNPDSIEIEYIQSDSRKTNKNDIFCVADSIGSKKKEF
CCCHHHHHHHHCCCCEEECCCCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCCHHHHH
ISNAKASLILLRTDSNVLNDLLEVMNSSKIFLECEIDPEQLQGRIASFLLGHPSKDLDIV
HCCCCEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCEEE
AVTGTNGKTSLTNILFSLAKDQGINCGLIGTIGVKFGDRVIDTGYTTPDASSLNLILKEM
EEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHH
KEEGITTVFMEASSHGLKLGRMNGISVRAGVFTNLTQDHLDFHSDMEDYFESKFRLFEIL
HHCCCEEEEEECCCCCEEEECCCCCEEECCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
DFSKSTFAVLDYSAPNGSKLYHKILNRFPDLLIYALDDIYRKWKISDISLNLQGTSYVLG
CCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCEEEEE
LPGNQERKISTNLLGSFNVRNTALAFLTGIGIGLDLEKMSNSLEKIPQIPGRFQIIYSKD
CCCCCCCEEHHHHCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCEEEEEECC
RSRMAVVDYAHTPDALENIIRSVRDSQPKCLITLFGCGGDRDRTKRPKMARIAEELSDQV
CCEEEEEEECCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHCCCE
ILTSDNPRTEKPETILDEIQTGFSSGFIPLLREVDRAKAIVEGISCLPEGGCLLVAGKGH
EEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCC
EEYQIIGKEKRHFSDVEEVQKAFGLF
CCEEEECCHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA