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The map label for this gene is prfC

Identifier: 45657162

GI number: 45657162

Start: 1579160

End: 1580872

Strand: Direct

Name: prfC

Synonym: LIC11280

Alternate gene names: 45657162

Gene position: 1579160-1580872 (Clockwise)

Preceding gene: 45657161

Following gene: 45657163

Centisome position: 36.92

GC content: 39.99

Gene sequence:

>1713_bases
ATGGCACTCAACTTGGTTGGGATCAGTAGAAGCAATTGCCTATTCGGTTTAGTGGATGACAGTCGGTTTTATAAAAAAAA
TCTGGAAGGAGGGCGGAGCAAAATAGTGAGTGATACAGTGGAACAAAAATCAAATCGAACTATCGAGGAAGAAACTCGTA
GAAGAAGAACCTTCGCGATTATTGCCCACCCTGACGCAGGAAAAACGACTCTTACAGAAAAACTTCTATTGTATGGAGGT
GCGATTCAACTCGCTGGAGCGGTAAAAGCCCGAAAAAATCGAAAGGCTGCTACTTCCGATTGGATGGAGATGGAAAAAGA
AAAAGGAATTTCTATTACTTCCGCAGCGCTTCAGTTTGAATATTCTGGACACGTTCTCAATTTATTGGATACACCCGGTC
ACGAAGATTTTTCAGAAGATACATATCGCACTTTGATCGCAGCGGATACCGCAGTGATGGTGTTAGACGCAGGTAAAGGA
GTAGAACCTCAAACGATTAAGTTGTTTAAAGTTTGTAGAGATCGTGGAATTCCAATCGTAACCTTCATCAACAAAATGGA
TAGACCAACTAAAAATCTTTTTTCTCTTTTAGATGAAATCGAAAAGGTTCTAGAAATTTCAGCTGTGCCAATGGTATGGC
CGATTGGAACTGGAGTCGATTTTAGTGGAGTTTATTCCCGCAAAGATAAGAAAATTCTAACATACGATAAAACTCCTGGA
GGAAGTCAGAAGTCTACTTTTCAAACCTCCGGTGTAAATGATCCCGAGTTAGATTCTAGATTTGAAGACTGGGTGATCAA
AACCTTTCGGGAAGAATTGGAGTTGGTAGAAGGCGGAATCTCAGAATTCAGTCAGGAAGATTTTTTAGATTCTAAAATCA
CTCCTGTTTTTTTTGGTTCTGCCGTGAACAATTTTGGGATTCAATTATTTTTAGACGAATTTATTAAGATCGCTCCTCCT
CCTTTATTTTTTCCTTTAAAAGACGGATCTAAATTGGATCCGATACAGACGCCGTTCAGCGGATTTATATTTAAGGTTCA
AGCAAATATGAATCGGCAACATAGGGATCGAATTGCATTTTTGCGTGTGACGTCTGGTAAGTTTGAAAGAGGACTTAACG
TGCTTCATGGAAGGCTTGGAAAATCGGTGAAACTTTCATCTTCTTTTGCGTTTTTTGGCCAAGACAGAAACACCGTAGAT
GAGGCTTATCCGGGAGACATCATCGGTTTAGTAAATCCTGGAACGTATGCGATTGGGGACATTGTAGCTTCTTCTAAGGT
TCCTGATTTAAAATCTCTTCCTGTATTTGCGCCTGAACTTTTTGCTACGATTTCTTCTTCCGACACCTCAAGTATGAAAA
GTTTTCGAAAAGGTATTGATCAATTAGCGGAAGAGGGAATTCTCCATTTATTTTCTTCTCAGACGATTGGAGGGGGACTG
CCCATTATTGGAGCAATGGGGCAACTTCAGTTTGAAGTTTTTAGAAGAAGACTTTTAGATGAATACAACGCTCCTTCTAC
GATTACGATTTTACCTTATGTAATTTCCTGTTGGATTGGTCAGGAAGATTTAGGAAAGGTTCCTTCTTCTGCAAACCTTG
TGACAGATCGAGGCGGTAGGGCGGCTCTTCTTTTTGATACCGAGTGGGATAAGGGATATTTTCAAAAGAAAAATCCGGAG
ATTACCCTTTTAGATTATCCACCTAGTATTTGA

Upstream 100 bases:

>100_bases
GATGAACTCAAGATAACTAGATTAAACTTTTTATTACTCGGAACTATAAAATAAAGTTCCCAGTATTCTACCCTGAAACA
GTACTTTGCAATAAAAATTT

Downstream 100 bases:

>100_bases
GTAAATTTTTAAAAGTAGAATCATCTCAAAAATAGCAGATGAGCATTTTTTTAATACAAATGGGATGATAGATTAATTTT
TATAGAATTTAAGTATTATA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MALNLVGISRSNCLFGLVDDSRFYKKNLEGGRSKIVSDTVEQKSNRTIEEETRRRRTFAIIAHPDAGKTTLTEKLLLYGG
AIQLAGAVKARKNRKAATSDWMEMEKEKGISITSAALQFEYSGHVLNLLDTPGHEDFSEDTYRTLIAADTAVMVLDAGKG
VEPQTIKLFKVCRDRGIPIVTFINKMDRPTKNLFSLLDEIEKVLEISAVPMVWPIGTGVDFSGVYSRKDKKILTYDKTPG
GSQKSTFQTSGVNDPELDSRFEDWVIKTFREELELVEGGISEFSQEDFLDSKITPVFFGSAVNNFGIQLFLDEFIKIAPP
PLFFPLKDGSKLDPIQTPFSGFIFKVQANMNRQHRDRIAFLRVTSGKFERGLNVLHGRLGKSVKLSSSFAFFGQDRNTVD
EAYPGDIIGLVNPGTYAIGDIVASSKVPDLKSLPVFAPELFATISSSDTSSMKSFRKGIDQLAEEGILHLFSSQTIGGGL
PIIGAMGQLQFEVFRRRLLDEYNAPSTITILPYVISCWIGQEDLGKVPSSANLVTDRGGRAALLFDTEWDKGYFQKKNPE
ITLLDYPPSI

Sequences:

>Translated_570_residues
MALNLVGISRSNCLFGLVDDSRFYKKNLEGGRSKIVSDTVEQKSNRTIEEETRRRRTFAIIAHPDAGKTTLTEKLLLYGG
AIQLAGAVKARKNRKAATSDWMEMEKEKGISITSAALQFEYSGHVLNLLDTPGHEDFSEDTYRTLIAADTAVMVLDAGKG
VEPQTIKLFKVCRDRGIPIVTFINKMDRPTKNLFSLLDEIEKVLEISAVPMVWPIGTGVDFSGVYSRKDKKILTYDKTPG
GSQKSTFQTSGVNDPELDSRFEDWVIKTFREELELVEGGISEFSQEDFLDSKITPVFFGSAVNNFGIQLFLDEFIKIAPP
PLFFPLKDGSKLDPIQTPFSGFIFKVQANMNRQHRDRIAFLRVTSGKFERGLNVLHGRLGKSVKLSSSFAFFGQDRNTVD
EAYPGDIIGLVNPGTYAIGDIVASSKVPDLKSLPVFAPELFATISSSDTSSMKSFRKGIDQLAEEGILHLFSSQTIGGGL
PIIGAMGQLQFEVFRRRLLDEYNAPSTITILPYVISCWIGQEDLGKVPSSANLVTDRGGRAALLFDTEWDKGYFQKKNPE
ITLLDYPPSI
>Mature_569_residues
ALNLVGISRSNCLFGLVDDSRFYKKNLEGGRSKIVSDTVEQKSNRTIEEETRRRRTFAIIAHPDAGKTTLTEKLLLYGGA
IQLAGAVKARKNRKAATSDWMEMEKEKGISITSAALQFEYSGHVLNLLDTPGHEDFSEDTYRTLIAADTAVMVLDAGKGV
EPQTIKLFKVCRDRGIPIVTFINKMDRPTKNLFSLLDEIEKVLEISAVPMVWPIGTGVDFSGVYSRKDKKILTYDKTPGG
SQKSTFQTSGVNDPELDSRFEDWVIKTFREELELVEGGISEFSQEDFLDSKITPVFFGSAVNNFGIQLFLDEFIKIAPPP
LFFPLKDGSKLDPIQTPFSGFIFKVQANMNRQHRDRIAFLRVTSGKFERGLNVLHGRLGKSVKLSSSFAFFGQDRNTVDE
AYPGDIIGLVNPGTYAIGDIVASSKVPDLKSLPVFAPELFATISSSDTSSMKSFRKGIDQLAEEGILHLFSSQTIGGGLP
IIGAMGQLQFEVFRRRLLDEYNAPSTITILPYVISCWIGQEDLGKVPSSANLVTDRGGRAALLFDTEWDKGYFQKKNPEI
TLLDYPPSI

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=495, Percent_Identity=29.6969696969697, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI19923640, Length=492, Percent_Identity=27.0325203252033, Blast_Score=143, Evalue=4e-34,
Organism=Homo sapiens, GI25306283, Length=387, Percent_Identity=29.1989664082687, Blast_Score=142, Evalue=1e-33,
Organism=Homo sapiens, GI25306287, Length=286, Percent_Identity=33.2167832167832, Blast_Score=135, Evalue=8e-32,
Organism=Homo sapiens, GI157426893, Length=172, Percent_Identity=33.7209302325581, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI94966754, Length=147, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=4e-15,
Organism=Homo sapiens, GI4503483, Length=170, Percent_Identity=32.9411764705882, Blast_Score=77, Evalue=6e-14,
Organism=Homo sapiens, GI310132016, Length=123, Percent_Identity=34.1463414634146, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI310110807, Length=123, Percent_Identity=34.1463414634146, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI310123363, Length=123, Percent_Identity=34.1463414634146, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790835, Length=518, Percent_Identity=44.4015444015444, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI1789738, Length=488, Percent_Identity=28.8934426229508, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1788922, Length=178, Percent_Identity=35.3932584269663, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI48994988, Length=165, Percent_Identity=33.3333333333333, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=495, Percent_Identity=26.0606060606061, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=477, Percent_Identity=26.4150943396226, Blast_Score=140, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17557151, Length=150, Percent_Identity=34, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=166, Percent_Identity=33.7349397590361, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=135, Percent_Identity=30.3703703703704, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=135, Percent_Identity=30.3703703703704, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=499, Percent_Identity=28.6573146292585, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322359, Length=510, Percent_Identity=28.6274509803922, Blast_Score=143, Evalue=8e-35,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=29.9363057324841, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6324707, Length=170, Percent_Identity=32.3529411764706, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=170, Percent_Identity=32.3529411764706, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324166, Length=165, Percent_Identity=29.0909090909091, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319282, Length=143, Percent_Identity=34.965034965035, Blast_Score=64, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24582462, Length=480, Percent_Identity=29.1666666666667, Blast_Score=189, Evalue=3e-48,
Organism=Drosophila melanogaster, GI221458488, Length=497, Percent_Identity=25.7545271629779, Blast_Score=159, Evalue=7e-39,
Organism=Drosophila melanogaster, GI78706572, Length=178, Percent_Identity=34.8314606741573, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585709, Length=163, Percent_Identity=33.7423312883436, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24585711, Length=159, Percent_Identity=33.9622641509434, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24585713, Length=159, Percent_Identity=33.9622641509434, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI28574573, Length=173, Percent_Identity=32.3699421965318, Blast_Score=71, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24656849, Length=158, Percent_Identity=30.379746835443, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 63242; Mature: 63111

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALNLVGISRSNCLFGLVDDSRFYKKNLEGGRSKIVSDTVEQKSNRTIEEETRRRRTFAI
CEEEEEEECCCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHCCEEEEE
IAHPDAGKTTLTEKLLLYGGAIQLAGAVKARKNRKAATSDWMEMEKEKGISITSAALQFE
EECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEEEEE
YSGHVLNLLDTPGHEDFSEDTYRTLIAADTAVMVLDAGKGVEPQTIKLFKVCRDRGIPIV
ECCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEE
TFINKMDRPTKNLFSLLDEIEKVLEISAVPMVWPIGTGVDFSGVYSRKDKKILTYDKTPG
EHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCC
GSQKSTFQTSGVNDPELDSRFEDWVIKTFREELELVEGGISEFSQEDFLDSKITPVFFGS
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEECC
AVNNFGIQLFLDEFIKIAPPPLFFPLKDGSKLDPIQTPFSGFIFKVQANMNRQHRDRIAF
HHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCHHHCCEEEE
LRVTSGKFERGLNVLHGRLGKSVKLSSSFAFFGQDRNTVDEAYPGDIIGLVNPGTYAIGD
EEEECCCHHHHHHHHHHHCCCCEEECCCEEEECCCCCCHHHCCCCCEEEEECCCCCHHHH
IVASSKVPDLKSLPVFAPELFATISSSDTSSMKSFRKGIDQLAEEGILHLFSSQTIGGGL
HHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PIIGAMGQLQFEVFRRRLLDEYNAPSTITILPYVISCWIGQEDLGKVPSSANLVTDRGGR
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCHHHCCCCCCCCEEECCCCC
AALLFDTEWDKGYFQKKNPEITLLDYPPSI
EEEEEECCCCCCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure 
ALNLVGISRSNCLFGLVDDSRFYKKNLEGGRSKIVSDTVEQKSNRTIEEETRRRRTFAI
EEEEEEECCCCCEEEEECCCHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHCCEEEEE
IAHPDAGKTTLTEKLLLYGGAIQLAGAVKARKNRKAATSDWMEMEKEKGISITSAALQFE
EECCCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEEEEEE
YSGHVLNLLDTPGHEDFSEDTYRTLIAADTAVMVLDAGKGVEPQTIKLFKVCRDRGIPIV
ECCCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCEEE
TFINKMDRPTKNLFSLLDEIEKVLEISAVPMVWPIGTGVDFSGVYSRKDKKILTYDKTPG
EHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEEECCCCC
GSQKSTFQTSGVNDPELDSRFEDWVIKTFREELELVEGGISEFSQEDFLDSKITPVFFGS
CCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEECC
AVNNFGIQLFLDEFIKIAPPPLFFPLKDGSKLDPIQTPFSGFIFKVQANMNRQHRDRIAF
HHHHCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCEEEEEEECCCHHHCCEEEE
LRVTSGKFERGLNVLHGRLGKSVKLSSSFAFFGQDRNTVDEAYPGDIIGLVNPGTYAIGD
EEEECCCHHHHHHHHHHHCCCCEEECCCEEEECCCCCCHHHCCCCCEEEEECCCCCHHHH
IVASSKVPDLKSLPVFAPELFATISSSDTSSMKSFRKGIDQLAEEGILHLFSSQTIGGGL
HHHCCCCCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PIIGAMGQLQFEVFRRRLLDEYNAPSTITILPYVISCWIGQEDLGKVPSSANLVTDRGGR
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHCCCHHHCCCCCCCCEEECCCCC
AALLFDTEWDKGYFQKKNPEITLLDYPPSI
EEEEEECCCCCCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA