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The map label for this gene is ilvD

Identifier: 45656985

GI number: 45656985

Start: 1357501

End: 1359183

Strand: Direct

Name: ilvD

Synonym: LIC11101

Alternate gene names: 45656985

Gene position: 1357501-1359183 (Clockwise)

Preceding gene: 45656984

Following gene: 45656991

Centisome position: 31.74

GC content: 43.85

Gene sequence:

>1683_bases
ATGGGTGATAATCTAAAAAAAAGAAGTTCGATGACTACGGATGGAGACAATCGCGCTCCCAATAGAGCGATGCTTCGCGC
GGTTGGATTTACGGACGAAGACTTTCACAAACCGATGATAGGAATCGCGTCTACTTGGAGTGAAATCACACCTTGCAATA
TTCATATCAATAAACTCGCAGAAAAAGTTAAAGAAGGTGTTCGAGAAGCGGGAGGAGTGCCTCAGATCTACGGAACCATC
ACCGTTTCCGACGGAATCATGATGGGTCATGAGGGAATGCACTTCTCTCTTCCTTCCAGAGAAGTGATCGCGGACTCGAT
CGAAATCGTATCTAACGCAATGAGACACGATGGAGTGATTGCGATCGGAGGTTGTGATAAGAATATGCCAGGGTGTCTCA
TGGCTCTTTGTAGAATTGATGCACCTTCTATTTTTGTCTATGGAGGAACGATTCTTCCCGGAAACTGCGATGGTCAGGAC
GTGGATATCGTTTCCATTTTCGAAGCGGTCGGAAAATTCAACGCTGGAAAAATCTCCAGAGAAGAATTTATCCGAATCGA
ACAAAATGCAATTCCGGGTGCCGGAAGCTGTGGTGGAATGTATACCGCTAACACAATGTCTTCCGCGATTGAGGCGTTGG
GAATGAGTCTTCCCGGTTCCGCCTCTATGCCCGCAGTAAGTTCCAGAAAGGCAAACGACTGCTACGAAGCTGGAAAGGCT
TTGATCAACCTAATTCAAAAAGGAATCACTCCCAAACAAATTCTGACTAAAAAAGCATTTGAAAATGCGATCACCGTAGT
GCTCGTGTTAGGCGGTTCCACCAATGCGGTTCTTCATTTGATAGCGATTGCCAAAGAAATTGGAGTGGGCCTGACTCTGG
ACGATTTTGACCGAATTAGTAAAAAAACTCCTCATTTAGCCGATTTAAAGCCGGGCGGAAAATACGCCATGACTGATCTT
GACAAAGTAGGAGGGGTCCATGGTGTGATGAAGTATCTTTTAAAAGAAGGAATGCTTCATGGAGATTGTTTAACGGTTAC
CGGAAAAACCATTGCAGAAAATCTAAAGGACATGCCCGACTTGGTTCCAAATCAAACTATAGTTCGCAAAAAATCAGAGG
CTTTACATCCTTCGGGTCCACTTGTAATTTTAAAAGGAAACTTAGCTCCAGACGGAGCGGTCGCAAAAATTTCTGGTTTG
AAAAAAATTTCCATCACTGGTCCTGCAAAAGTTTTTGAGTCCGAAGACGATTGTTTTAACGCGATTATGACTGACAAAAT
CAAACCGGGCGACGTGATCATCATTCGTTACGAAGGTCCAAAAGGTGGTCCTGGAATGAGGGAAATGCTCGCTGTAACTT
CCGCTCTTGTTGGCAAAGGATTGGGAGAAGACGTTGGATTGATGACGGACGGAAGATTTAGCGGAGGAACACACGGTCTT
GTAGTAGGACATATCTCTCCCGAAGCATTTGATGGAGGGCCGATTGCGATCATTCAAAATGGAGATAAGGTAACTATTGA
TTCTAGTAAGAATTTACTCCAAGTCGAAATTTCTCAAGAGGAAATTGACAAACGATTGAAGAGCTGGAAACCGATAGAAC
CTAGATATAAAACTGGCGTTCTTGCTAAATACGTAAAACTGGTTCAATCGGCGACTAATGGAGCAATTACCAATCTACTT
TAA

Upstream 100 bases:

>100_bases
TTACAATACAAATTGACCGGAAAGATCGTTCGCAAAACTGTACTTTTAAGTCTGTTCCAACAAGAACGATTAGAAACGGC
TAAAAATAGAGGCTTTCAAC

Downstream 100 bases:

>100_bases
ATCTCAGTTAACTTATTGAAGATAAAAATATCACCTAACTTAAGTTAGGTGATATTTTCTATTTAACGTGAGTTCGGTAT
ACGAATGCGAAAGCGATTGT

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD [H]

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MGDNLKKRSSMTTDGDNRAPNRAMLRAVGFTDEDFHKPMIGIASTWSEITPCNIHINKLAEKVKEGVREAGGVPQIYGTI
TVSDGIMMGHEGMHFSLPSREVIADSIEIVSNAMRHDGVIAIGGCDKNMPGCLMALCRIDAPSIFVYGGTILPGNCDGQD
VDIVSIFEAVGKFNAGKISREEFIRIEQNAIPGAGSCGGMYTANTMSSAIEALGMSLPGSASMPAVSSRKANDCYEAGKA
LINLIQKGITPKQILTKKAFENAITVVLVLGGSTNAVLHLIAIAKEIGVGLTLDDFDRISKKTPHLADLKPGGKYAMTDL
DKVGGVHGVMKYLLKEGMLHGDCLTVTGKTIAENLKDMPDLVPNQTIVRKKSEALHPSGPLVILKGNLAPDGAVAKISGL
KKISITGPAKVFESEDDCFNAIMTDKIKPGDVIIIRYEGPKGGPGMREMLAVTSALVGKGLGEDVGLMTDGRFSGGTHGL
VVGHISPEAFDGGPIAIIQNGDKVTIDSSKNLLQVEISQEEIDKRLKSWKPIEPRYKTGVLAKYVKLVQSATNGAITNLL

Sequences:

>Translated_560_residues
MGDNLKKRSSMTTDGDNRAPNRAMLRAVGFTDEDFHKPMIGIASTWSEITPCNIHINKLAEKVKEGVREAGGVPQIYGTI
TVSDGIMMGHEGMHFSLPSREVIADSIEIVSNAMRHDGVIAIGGCDKNMPGCLMALCRIDAPSIFVYGGTILPGNCDGQD
VDIVSIFEAVGKFNAGKISREEFIRIEQNAIPGAGSCGGMYTANTMSSAIEALGMSLPGSASMPAVSSRKANDCYEAGKA
LINLIQKGITPKQILTKKAFENAITVVLVLGGSTNAVLHLIAIAKEIGVGLTLDDFDRISKKTPHLADLKPGGKYAMTDL
DKVGGVHGVMKYLLKEGMLHGDCLTVTGKTIAENLKDMPDLVPNQTIVRKKSEALHPSGPLVILKGNLAPDGAVAKISGL
KKISITGPAKVFESEDDCFNAIMTDKIKPGDVIIIRYEGPKGGPGMREMLAVTSALVGKGLGEDVGLMTDGRFSGGTHGL
VVGHISPEAFDGGPIAIIQNGDKVTIDSSKNLLQVEISQEEIDKRLKSWKPIEPRYKTGVLAKYVKLVQSATNGAITNLL
>Mature_559_residues
GDNLKKRSSMTTDGDNRAPNRAMLRAVGFTDEDFHKPMIGIASTWSEITPCNIHINKLAEKVKEGVREAGGVPQIYGTIT
VSDGIMMGHEGMHFSLPSREVIADSIEIVSNAMRHDGVIAIGGCDKNMPGCLMALCRIDAPSIFVYGGTILPGNCDGQDV
DIVSIFEAVGKFNAGKISREEFIRIEQNAIPGAGSCGGMYTANTMSSAIEALGMSLPGSASMPAVSSRKANDCYEAGKAL
INLIQKGITPKQILTKKAFENAITVVLVLGGSTNAVLHLIAIAKEIGVGLTLDDFDRISKKTPHLADLKPGGKYAMTDLD
KVGGVHGVMKYLLKEGMLHGDCLTVTGKTIAENLKDMPDLVPNQTIVRKKSEALHPSGPLVILKGNLAPDGAVAKISGLK
KISITGPAKVFESEDDCFNAIMTDKIKPGDVIIIRYEGPKGGPGMREMLAVTSALVGKGLGEDVGLMTDGRFSGGTHGLV
VGHISPEAFDGGPIAIIQNGDKVTIDSSKNLLQVEISQEEIDKRLKSWKPIEPRYKTGVLAKYVKLVQSATNGAITNLL

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family [H]

Homologues:

Organism=Escherichia coli, GI48994964, Length=608, Percent_Identity=39.1447368421053, Blast_Score=412, Evalue=1e-116,
Organism=Escherichia coli, GI2367371, Length=469, Percent_Identity=34.9680170575693, Blast_Score=217, Evalue=1e-57,
Organism=Escherichia coli, GI1786464, Length=481, Percent_Identity=32.8482328482328, Blast_Score=202, Evalue=6e-53,
Organism=Escherichia coli, GI1788157, Length=508, Percent_Identity=27.755905511811, Blast_Score=192, Evalue=3e-50,
Organism=Saccharomyces cerevisiae, GI6322476, Length=560, Percent_Identity=48.9285714285714, Blast_Score=541, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558 [H]

Pfam domain/function: PF00920 ILVD_EDD [H]

EC number: =4.2.1.9 [H]

Molecular weight: Translated: 59417; Mature: 59285

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS00886 ILVD_EDD_1 ; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDNLKKRSSMTTDGDNRAPNRAMLRAVGFTDEDFHKPMIGIASTWSEITPCNIHINKLA
CCCCCCHHCCCCCCCCCCCCCHHHHEEECCCCHHHHCCHHHHHHHHHHCCCCEEEHHHHH
EKVKEGVREAGGVPQIYGTITVSDGIMMGHEGMHFSLPSREVIADSIEIVSNAMRHDGVI
HHHHHHHHHHCCCCEEEEEEEEECCEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCEE
AIGGCDKNMPGCLMALCRIDAPSIFVYGGTILPGNCDGQDVDIVSIFEAVGKFNAGKISR
EECCCCCCCHHHHHHHHHCCCCEEEEECCEECCCCCCCCCCHHHHHHHHHCCCCCCCCCH
EEFIRIEQNAIPGAGSCGGMYTANTMSSAIEALGMSLPGSASMPAVSSRKANDCYEAGKA
HHHHEECCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
LINLIQKGITPKQILTKKAFENAITVVLVLGGSTNAVLHLIAIAKEIGVGLTLDDFDRIS
HHHHHHHCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
KKTPHLADLKPGGKYAMTDLDKVGGVHGVMKYLLKEGMLHGDCLTVTGKTIAENLKDMPD
CCCCCCCCCCCCCCEECCCHHHHCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHH
LVPNQTIVRKKSEALHPSGPLVILKGNLAPDGAVAKISGLKKISITGPAKVFESEDDCFN
HCCCCHHHHCHHHCCCCCCCEEEEECCCCCCCCEEEECCCEEEEECCCHHHHCCCCHHHH
AIMTDKIKPGDVIIIRYEGPKGGPGMREMLAVTSALVGKGLGEDVGLMTDGRFSGGTHGL
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCE
VVGHISPEAFDGGPIAIIQNGDKVTIDSSKNLLQVEISQEEIDKRLKSWKPIEPRYKTGV
EEEECCCCCCCCCCEEEEECCCEEEEECCCCEEEEEECHHHHHHHHHCCCCCCCCHHHHH
LAKYVKLVQSATNGAITNLL
HHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
GDNLKKRSSMTTDGDNRAPNRAMLRAVGFTDEDFHKPMIGIASTWSEITPCNIHINKLA
CCCCCHHCCCCCCCCCCCCCHHHHEEECCCCHHHHCCHHHHHHHHHHCCCCEEEHHHHH
EKVKEGVREAGGVPQIYGTITVSDGIMMGHEGMHFSLPSREVIADSIEIVSNAMRHDGVI
HHHHHHHHHHCCCCEEEEEEEEECCEEECCCCCEECCCCHHHHHHHHHHHHHHHHHCCEE
AIGGCDKNMPGCLMALCRIDAPSIFVYGGTILPGNCDGQDVDIVSIFEAVGKFNAGKISR
EECCCCCCCHHHHHHHHHCCCCEEEEECCEECCCCCCCCCCHHHHHHHHHCCCCCCCCCH
EEFIRIEQNAIPGAGSCGGMYTANTMSSAIEALGMSLPGSASMPAVSSRKANDCYEAGKA
HHHHEECCCCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
LINLIQKGITPKQILTKKAFENAITVVLVLGGSTNAVLHLIAIAKEIGVGLTLDDFDRIS
HHHHHHHCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHH
KKTPHLADLKPGGKYAMTDLDKVGGVHGVMKYLLKEGMLHGDCLTVTGKTIAENLKDMPD
CCCCCCCCCCCCCCEECCCHHHHCCHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHH
LVPNQTIVRKKSEALHPSGPLVILKGNLAPDGAVAKISGLKKISITGPAKVFESEDDCFN
HCCCCHHHHCHHHCCCCCCCEEEEECCCCCCCCEEEECCCEEEEECCCHHHHCCCCHHHH
AIMTDKIKPGDVIIIRYEGPKGGPGMREMLAVTSALVGKGLGEDVGLMTDGRFSGGTHGL
HHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCE
VVGHISPEAFDGGPIAIIQNGDKVTIDSSKNLLQVEISQEEIDKRLKSWKPIEPRYKTGV
EEEECCCCCCCCCCEEEEECCCEEEEECCCCEEEEEECHHHHHHHHHCCCCCCCCHHHHH
LAKYVKLVQSATNGAITNLL
HHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA