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The map label for this gene is metK

Identifier: 161621776

GI number: 161621776

Start: 1667918

End: 1669078

Strand: Direct

Name: metK

Synonym: LIC11354

Alternate gene names: 161621776

Gene position: 1667918-1669078 (Clockwise)

Preceding gene: 45657229

Following gene: 45657233

Centisome position: 39.0

GC content: 40.4

Gene sequence:

>1161_bases
ATGTCTTTAAAAGATTTTATTTTTACTTCGGAATCAGTTGGCGAAGGTCATCCGGACAAGGTTTGTGACCAGATATCCGA
TGCAGTTTTAGATGCTTATCTTGAACAGGATCCAAAATCTAGAGTTGCCTGCGAGACCTTAGTGACCACAAATCTGGTCG
TAATTGCAGGTGAAATTACAAGTAAGGGAAAAGTAGACGCACAAGAAATTGCAAGAAATGTAATTCGAGATATAGGTTAT
AATGATATTACGATGGGATTTGATGCCGACTTTGCGGTAGTTTCCGCGCATGTTCATGCACAAAGCCCGGATATTTCTCA
AGGCGTAACTGAGGGAGAAGGTCTTTTCAAAGAACAAGGTGCAGGTGATCAAGGTTTGATGTTCGGTTTTGCAATCAATG
AAACTCCGGAATTGATGCCTATGCCAATCTACTATTCTCATGAGCTAGTTAAACATTTAGCTGGACTAAGACATGGTAAT
AAACTGAAGTTTTTAAGACCCGATGCAAAGTCTCAGGTTACGGTAGAATATAAAGACGGAAAACCGGTTCGTATAGACAC
GGTGGTAATTTCTACACAACATTCTCCGGATGTAACTCATAAACAAATTGAAGAAGCTTTGATCGAAGAATGTATCAAGA
AAGTGATTCCTGCAAATCTTCTGAACAATACGAAATATTTTATTAACCCTACTGGTCAGTTTATTATTGGGGGACCTCAT
GGAGACGCGGGGTTAACTGGTAGAAAGATCATTGTGGATACATACGGTGGATATGGAAGACACGGAGGAGGTGCATTCTC
CGGTAAGGATCCTTCTAAAGTAGATCGTTCAGCGGCTTATATGGGACGTTATATTGCAAAGAACGTAGTGGCTTCGGGAC
TTGCTGACAAATGTGAAGTGCAGCTTGCTTACGCGATTGGTGTTGCAGAACCAGTTTCAGTTCATGTGGACACGTTTGGA
ACCGGAAAAATTTCCGAAGAAGAATTGGTAAAAAGAATTCGAGCTAATTTCAAACTAACTCCAAGAGGAATTATAGAATC
TCTCAAACTTCTCGAAAAAGGAAGAAAGTATAGAGAAACCGCTTCTTATGGGCATTTCGGAAGAAAGGGTTCCACTTTTA
CTTGGGAAGAAACCGACAAAGCCTCTGCTTTAAAAGGATAA

Upstream 100 bases:

>100_bases
AAGGAACATAGTTTGGATCTTCTTGACACCAAGGCAATCCGTAGGTTTTTGGGATCAGAGCGGAACTTCTCCGTTAATTT
TTCACCTTCAGGAAGAACAG

Downstream 100 bases:

>100_bases
TATAAAAATGGGAGCTCCAAGCACATCGACTGCGACTGAAAAAGCTACTCGTGATGGTTATGGAGACGCGCTGCATGAAT
TAGGTGCTTCTCGTCCGGAT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MSLKDFIFTSESVGEGHPDKVCDQISDAVLDAYLEQDPKSRVACETLVTTNLVVIAGEITSKGKVDAQEIARNVIRDIGY
NDITMGFDADFAVVSAHVHAQSPDISQGVTEGEGLFKEQGAGDQGLMFGFAINETPELMPMPIYYSHELVKHLAGLRHGN
KLKFLRPDAKSQVTVEYKDGKPVRIDTVVISTQHSPDVTHKQIEEALIEECIKKVIPANLLNNTKYFINPTGQFIIGGPH
GDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVASGLADKCEVQLAYAIGVAEPVSVHVDTFG
TGKISEEELVKRIRANFKLTPRGIIESLKLLEKGRKYRETASYGHFGRKGSTFTWEETDKASALKG

Sequences:

>Translated_386_residues
MSLKDFIFTSESVGEGHPDKVCDQISDAVLDAYLEQDPKSRVACETLVTTNLVVIAGEITSKGKVDAQEIARNVIRDIGY
NDITMGFDADFAVVSAHVHAQSPDISQGVTEGEGLFKEQGAGDQGLMFGFAINETPELMPMPIYYSHELVKHLAGLRHGN
KLKFLRPDAKSQVTVEYKDGKPVRIDTVVISTQHSPDVTHKQIEEALIEECIKKVIPANLLNNTKYFINPTGQFIIGGPH
GDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVASGLADKCEVQLAYAIGVAEPVSVHVDTFG
TGKISEEELVKRIRANFKLTPRGIIESLKLLEKGRKYRETASYGHFGRKGSTFTWEETDKASALKG
>Mature_385_residues
SLKDFIFTSESVGEGHPDKVCDQISDAVLDAYLEQDPKSRVACETLVTTNLVVIAGEITSKGKVDAQEIARNVIRDIGYN
DITMGFDADFAVVSAHVHAQSPDISQGVTEGEGLFKEQGAGDQGLMFGFAINETPELMPMPIYYSHELVKHLAGLRHGNK
LKFLRPDAKSQVTVEYKDGKPVRIDTVVISTQHSPDVTHKQIEEALIEECIKKVIPANLLNNTKYFINPTGQFIIGGPHG
DAGLTGRKIIVDTYGGYGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVASGLADKCEVQLAYAIGVAEPVSVHVDTFGT
GKISEEELVKRIRANFKLTPRGIIESLKLLEKGRKYRETASYGHFGRKGSTFTWEETDKASALKG

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI4557737, Length=376, Percent_Identity=53.7234042553192, Blast_Score=378, Evalue=1e-105,
Organism=Homo sapiens, GI5174529, Length=376, Percent_Identity=52.9255319148936, Blast_Score=375, Evalue=1e-104,
Organism=Escherichia coli, GI1789311, Length=380, Percent_Identity=62.1052631578947, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI25145633, Length=385, Percent_Identity=54.5454545454545, Blast_Score=392, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17538494, Length=382, Percent_Identity=55.4973821989529, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17538498, Length=382, Percent_Identity=54.9738219895288, Blast_Score=390, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17551082, Length=385, Percent_Identity=54.5454545454545, Blast_Score=383, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI32565905, Length=382, Percent_Identity=50, Blast_Score=327, Evalue=6e-90,
Organism=Caenorhabditis elegans, GI32565911, Length=331, Percent_Identity=52.8700906344411, Blast_Score=315, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI25145635, Length=334, Percent_Identity=52.0958083832335, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI32565903, Length=304, Percent_Identity=52.9605263157895, Blast_Score=279, Evalue=2e-75,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=62.0689655172414, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32565907, Length=44, Percent_Identity=75, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320710, Length=382, Percent_Identity=52.8795811518325, Blast_Score=393, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323209, Length=377, Percent_Identity=54.1114058355438, Blast_Score=393, Evalue=1e-110,
Organism=Drosophila melanogaster, GI45552159, Length=377, Percent_Identity=51.4588859416446, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI24580529, Length=377, Percent_Identity=51.4588859416446, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI24580537, Length=377, Percent_Identity=51.4588859416446, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI24580533, Length=377, Percent_Identity=51.4588859416446, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI24580535, Length=377, Percent_Identity=51.4588859416446, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI24580531, Length=377, Percent_Identity=51.4588859416446, Blast_Score=357, Evalue=9e-99,
Organism=Drosophila melanogaster, GI24580527, Length=380, Percent_Identity=50.7894736842105, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580525, Length=380, Percent_Identity=50.7894736842105, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24580541, Length=367, Percent_Identity=49.591280653951, Blast_Score=329, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24580539, Length=122, Percent_Identity=51.6393442622951, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42034; Mature: 41903

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKDFIFTSESVGEGHPDKVCDQISDAVLDAYLEQDPKSRVACETLVTTNLVVIAGEIT
CCCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECCC
SKGKVDAQEIARNVIRDIGYNDITMGFDADFAVVSAHVHAQSPDISQGVTEGEGLFKEQG
CCCCCCHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEEECCCCCHHHCCCCCCCHHHHCC
AGDQGLMFGFAINETPELMPMPIYYSHELVKHLAGLRHGNKLKFLRPDAKSQVTVEYKDG
CCCCCEEEEEEECCCCCCEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCC
KPVRIDTVVISTQHSPDVTHKQIEEALIEECIKKVIPANLLNNTKYFINPTGQFIIGGPH
CCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCEEEECCCCCEEEECCC
GDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVASGLADKCEV
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEE
QLAYAIGVAEPVSVHVDTFGTGKISEEELVKRIRANFKLTPRGIIESLKLLEKGRKYRET
EEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHH
ASYGHFGRKGSTFTWEETDKASALKG
HCCCCCCCCCCEEEECCCCCHHCCCC
>Mature Secondary Structure 
SLKDFIFTSESVGEGHPDKVCDQISDAVLDAYLEQDPKSRVACETLVTTNLVVIAGEIT
CCHHHEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEEEEECCC
SKGKVDAQEIARNVIRDIGYNDITMGFDADFAVVSAHVHAQSPDISQGVTEGEGLFKEQG
CCCCCCHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEEECCCCCHHHCCCCCCCHHHHCC
AGDQGLMFGFAINETPELMPMPIYYSHELVKHLAGLRHGNKLKFLRPDAKSQVTVEYKDG
CCCCCEEEEEEECCCCCCEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCEEEEEECCC
KPVRIDTVVISTQHSPDVTHKQIEEALIEECIKKVIPANLLNNTKYFINPTGQFIIGGPH
CCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHCCCEEEECCCCCEEEECCC
GDAGLTGRKIIVDTYGGYGRHGGGAFSGKDPSKVDRSAAYMGRYIAKNVVASGLADKCEV
CCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHEE
QLAYAIGVAEPVSVHVDTFGTGKISEEELVKRIRANFKLTPRGIIESLKLLEKGRKYRET
EEEEEECCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHH
ASYGHFGRKGSTFTWEETDKASALKG
HCCCCCCCCCCEEEECCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA