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The map label for this gene is appA [H]

Identifier: 37680918

GI number: 37680918

Start: 2777535

End: 2779208

Strand: Direct

Name: appA [H]

Synonym: VV2734

Alternate gene names: 37680918

Gene position: 2777535-2779208 (Clockwise)

Preceding gene: 37680917

Following gene: 37680919

Centisome position: 82.8

GC content: 46.71

Gene sequence:

>1674_bases
ATGCTTGCCAATATTAAAAAGACAGCTCTAGCAACTGCAGTTATCGCCGCAGCAGCGACCAGCATTTCAGCACCAGCGGC
AGCTCGTAGTGAGCTGACCATCGTACCTGATTTTTACCCTACAATGGTACGTAACTTTAACCCGTACTTGGCGACTAACC
TTCGCACAACAACGGACTTCATCTATGAGCCACTAGTTGTTTTCAATGAGATGCACGGCAACACGCCAGTTATGCGTCTT
GCTGAAGACTTCCGCATGTCTGATGACCTAATGAGCGTAACGTTTGACATCCGTAAAGGTGTTAAATGGTCTGACGGTCA
AGCATTCAATGCTGACGACGTTGTGTTCTCTTATGCTCTACTTAAGAACAAACCAGAACTTGACCAACGTGGTATCAACA
AGTGGGTAACTAGCGTAGAGAAACTGAATGACTACCAAGTTCGTTTCCGTCTAAGCGAAGCTAACTCAAACGTACCTTAC
GAAATTTCTCTTGTTCCTATCGTTGCTGAGCACGTATGGAAAGAAGTGAAAGATCCAACAACATTCACGAACGAAAACCC
TGTAGGTACTGGTCCATTTACGGTTATCGACACCTTCACTCCTCAGCTATACATTCAGTGTCGTAACCCTAACTACTGGG
ATTCTGCGAACCTAGAAGTTGACTGTCTGCGTGTACCTCAGATCGCAAACAACGACCAGCTATTGGGTAAAATCGTTAAC
TCTGAGCTAGACTGGACTTCTTCTTTCATTCCAGATATTGACCGTACTTACGCAGCAGCGAGCCCTAACCATCAGTACTG
GTACCCACCATCAGGTACTCAAGCGTTCGTTGTGAACTTCAAGAACCCAGATGCAGCGAAGAACGAAGCTCTGACTAACG
TTGATTTCCGTCGTGCATTCTCAATGGCTCTTGACCGTCAAACTATCATCGACATCGCATTCTACGGTGGCGGTACAGTG
AACGACTTCGCATCTGGCCTTGGCTATGCATTCGAAGCGTGGTCTGACGAAAAGACTCACAACAAGTACAAAGGCTACAA
CACCTACAACGTTGAAGGCGCGAAGAAGCTTCTAGCGAAAGCTGGCTTTAAAGATGTGAACAAAGACGGTTTCGTTGATA
CTCCATCTGGCAAACCATTTGAGCTACTCATCCAATCTCCAAACGGTTGGACTGACTTCAACAACACAGTTCAACTAGCG
GTTGAGCAGCTTGCTGAAGTAGGTATTAAAGCACGTGCTCGTACTCCTGAGTTCGCAGTGTACAACCAAGCAATGCTTGA
TGGTACTTACGACGTGGCGTACACCAACTACTTCCACGGTGCGGATCCACACCTATACTGGAACAGTGCATACAACTCTG
CACTACAGAAAGGTGAAGGCATGCCACGCTTCGCGATGCACTTCTACACCAATGACAAACTGGACAACCTACTTGACAGC
TTCTACAAAACGGCTGACAAGAAAGAGCAGCTATCCATTGCTCATGGCATCCAGCAAATCATCGCAGCGGATCAAGTTAC
TATCCCTGTAATGTCTGGTGCTTACATGTACCAATACAACACCAAGCGCTTCACTGGTTGGTGGACAGAAGAAAATCCGA
AGGGCCGTCCAAACATTTGGGCAGGCATCCCAGAGCGTCTACTACACGTACTGGACCTAAAACCAGTTAAATAA

Upstream 100 bases:

>100_bases
ACTTTGGTAAATGATTATCAGAGGTAGGTCTTGGAGAGTGTTTATCAATTGATGACATTCCAAACATTGTGAAATGAAAG
CAAAATAGTAAGGAACAGCT

Downstream 100 bases:

>100_bases
GTAGAAGTTCATCTCCCGCGGCGCGCATCCCGTGCGCCGCTATAAAATCCCTCTCAGACCTAGTTTGTTTATGGCGCCAG
TCGTCAGGGGATTTTTCGCT

Product: ABC-type dipeptide transport system, periplasmic component

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 557; Mature: 557

Protein sequence:

>557_residues
MLANIKKTALATAVIAAAATSISAPAAARSELTIVPDFYPTMVRNFNPYLATNLRTTTDFIYEPLVVFNEMHGNTPVMRL
AEDFRMSDDLMSVTFDIRKGVKWSDGQAFNADDVVFSYALLKNKPELDQRGINKWVTSVEKLNDYQVRFRLSEANSNVPY
EISLVPIVAEHVWKEVKDPTTFTNENPVGTGPFTVIDTFTPQLYIQCRNPNYWDSANLEVDCLRVPQIANNDQLLGKIVN
SELDWTSSFIPDIDRTYAAASPNHQYWYPPSGTQAFVVNFKNPDAAKNEALTNVDFRRAFSMALDRQTIIDIAFYGGGTV
NDFASGLGYAFEAWSDEKTHNKYKGYNTYNVEGAKKLLAKAGFKDVNKDGFVDTPSGKPFELLIQSPNGWTDFNNTVQLA
VEQLAEVGIKARARTPEFAVYNQAMLDGTYDVAYTNYFHGADPHLYWNSAYNSALQKGEGMPRFAMHFYTNDKLDNLLDS
FYKTADKKEQLSIAHGIQQIIAADQVTIPVMSGAYMYQYNTKRFTGWWTEENPKGRPNIWAGIPERLLHVLDLKPVK

Sequences:

>Translated_557_residues
MLANIKKTALATAVIAAAATSISAPAAARSELTIVPDFYPTMVRNFNPYLATNLRTTTDFIYEPLVVFNEMHGNTPVMRL
AEDFRMSDDLMSVTFDIRKGVKWSDGQAFNADDVVFSYALLKNKPELDQRGINKWVTSVEKLNDYQVRFRLSEANSNVPY
EISLVPIVAEHVWKEVKDPTTFTNENPVGTGPFTVIDTFTPQLYIQCRNPNYWDSANLEVDCLRVPQIANNDQLLGKIVN
SELDWTSSFIPDIDRTYAAASPNHQYWYPPSGTQAFVVNFKNPDAAKNEALTNVDFRRAFSMALDRQTIIDIAFYGGGTV
NDFASGLGYAFEAWSDEKTHNKYKGYNTYNVEGAKKLLAKAGFKDVNKDGFVDTPSGKPFELLIQSPNGWTDFNNTVQLA
VEQLAEVGIKARARTPEFAVYNQAMLDGTYDVAYTNYFHGADPHLYWNSAYNSALQKGEGMPRFAMHFYTNDKLDNLLDS
FYKTADKKEQLSIAHGIQQIIAADQVTIPVMSGAYMYQYNTKRFTGWWTEENPKGRPNIWAGIPERLLHVLDLKPVK
>Mature_557_residues
MLANIKKTALATAVIAAAATSISAPAAARSELTIVPDFYPTMVRNFNPYLATNLRTTTDFIYEPLVVFNEMHGNTPVMRL
AEDFRMSDDLMSVTFDIRKGVKWSDGQAFNADDVVFSYALLKNKPELDQRGINKWVTSVEKLNDYQVRFRLSEANSNVPY
EISLVPIVAEHVWKEVKDPTTFTNENPVGTGPFTVIDTFTPQLYIQCRNPNYWDSANLEVDCLRVPQIANNDQLLGKIVN
SELDWTSSFIPDIDRTYAAASPNHQYWYPPSGTQAFVVNFKNPDAAKNEALTNVDFRRAFSMALDRQTIIDIAFYGGGTV
NDFASGLGYAFEAWSDEKTHNKYKGYNTYNVEGAKKLLAKAGFKDVNKDGFVDTPSGKPFELLIQSPNGWTDFNNTVQLA
VEQLAEVGIKARARTPEFAVYNQAMLDGTYDVAYTNYFHGADPHLYWNSAYNSALQKGEGMPRFAMHFYTNDKLDNLLDS
FYKTADKKEQLSIAHGIQQIIAADQVTIPVMSGAYMYQYNTKRFTGWWTEENPKGRPNIWAGIPERLLHVLDLKPVK

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=565, Percent_Identity=22.8318584070796, Blast_Score=79, Evalue=8e-16,
Organism=Escherichia coli, GI1787052, Length=467, Percent_Identity=23.5546038543897, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI1789887, Length=378, Percent_Identity=25.1322751322751, Blast_Score=71, Evalue=2e-13,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 62801; Mature: 62801

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLANIKKTALATAVIAAAATSISAPAAARSELTIVPDFYPTMVRNFNPYLATNLRTTTDF
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHCCCCCEECCCCHHHHH
IYEPLVVFNEMHGNTPVMRLAEDFRMSDDLMSVTFDIRKGVKWSDGQAFNADDVVFSYAL
HHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHEEHHHHCCCCCCCCCCCCCHHHHHHHHH
LKNKPELDQRGINKWVTSVEKLNDYQVRFRLSEANSNVPYEISLVPIVAEHVWKEVKDPT
HCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHCCCC
TFTNENPVGTGPFTVIDTFTPQLYIQCRNPNYWDSANLEVDCLRVPQIANNDQLLGKIVN
CCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHC
SELDWTSSFIPDIDRTYAAASPNHQYWYPPSGTQAFVVNFKNPDAAKNEALTNVDFRRAF
CCCCCHHHHCCCCHHHHCCCCCCCCEEECCCCCEEEEEEECCCCCCHHHHHCCHHHHHHH
SMALDRQTIIDIAFYGGGTVNDFASGLGYAFEAWSDEKTHNKYKGYNTYNVEGAKKLLAK
HHHHCCCEEEEEEEECCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCEECCHHHHHHHHH
AGFKDVNKDGFVDTPSGKPFELLIQSPNGWTDFNNTVQLAVEQLAEVGIKARARTPEFAV
CCCCCCCCCCCEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCCEE
YNQAMLDGTYDVAYTNYFHGADPHLYWNSAYNSALQKGEGMPRFAMHFYTNDKLDNLLDS
HHHHHHCCCEEEEEECCCCCCCCCEEECHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
FYKTADKKEQLSIAHGIQQIIAADQVTIPVMSGAYMYQYNTKRFTGWWTEENPKGRPNIW
HHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCEEEEEECCCCCCCCCCCC
AGIPERLLHVLDLKPVK
CCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MLANIKKTALATAVIAAAATSISAPAAARSELTIVPDFYPTMVRNFNPYLATNLRTTTDF
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHCCCCCEECCCCHHHHH
IYEPLVVFNEMHGNTPVMRLAEDFRMSDDLMSVTFDIRKGVKWSDGQAFNADDVVFSYAL
HHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHEEHHHHCCCCCCCCCCCCCHHHHHHHHH
LKNKPELDQRGINKWVTSVEKLNDYQVRFRLSEANSNVPYEISLVPIVAEHVWKEVKDPT
HCCCCCHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCEEEEEHHHHHHHHHHHHCCCC
TFTNENPVGTGPFTVIDTFTPQLYIQCRNPNYWDSANLEVDCLRVPQIANNDQLLGKIVN
CCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHC
SELDWTSSFIPDIDRTYAAASPNHQYWYPPSGTQAFVVNFKNPDAAKNEALTNVDFRRAF
CCCCCHHHHCCCCHHHHCCCCCCCCEEECCCCCEEEEEEECCCCCCHHHHHCCHHHHHHH
SMALDRQTIIDIAFYGGGTVNDFASGLGYAFEAWSDEKTHNKYKGYNTYNVEGAKKLLAK
HHHHCCCEEEEEEEECCCCHHHHHHHCCCEEECCCCCCCCCCCCCCCEECCHHHHHHHHH
AGFKDVNKDGFVDTPSGKPFELLIQSPNGWTDFNNTVQLAVEQLAEVGIKARARTPEFAV
CCCCCCCCCCCEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCCEE
YNQAMLDGTYDVAYTNYFHGADPHLYWNSAYNSALQKGEGMPRFAMHFYTNDKLDNLLDS
HHHHHHCCCEEEEEECCCCCCCCCEEECHHHHHHHHHCCCCCEEEEEEECCCHHHHHHHH
FYKTADKKEQLSIAHGIQQIIAADQVTIPVMSGAYMYQYNTKRFTGWWTEENPKGRPNIW
HHHCCCHHHHHHHHHHHHHHHHCCCEEEEEECCEEEEEECCCEEEEEECCCCCCCCCCCC
AGIPERLLHVLDLKPVK
CCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]