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The map label for this gene is proS [H]

Identifier: 37680756

GI number: 37680756

Start: 2599424

End: 2601139

Strand: Direct

Name: proS [H]

Synonym: VV2572

Alternate gene names: 37680756

Gene position: 2599424-2601139 (Clockwise)

Preceding gene: 37680755

Following gene: 37680757

Centisome position: 77.49

GC content: 49.36

Gene sequence:

>1716_bases
ATGCGTACCAGTAAATATCTTCTTTCTACTCTGAAGGAGACTCCAAACGACGCAGAAGTAGTGAGCCATCAGCTCATGCT
ACGTGCAGGTATGATCCGTAAGCTGGCTTCAGGTCTTTACACCTGGCTGCCTACCGGTCTACGTGTACTGCGTAAAGTCG
AAAACATCGTTCGTCAAGAGATCGACAATGCAGGTGCAATTGAAACCTTGATGCCCGTTGTGCAGCCGTTTGAACTATGG
GAAGAGACTGGCCGCTCTGAAAAAATGGGCCCGGAACTTCTTCGCTTTACTGACCGCCATGTTCGTCCGTTTGTACTCAG
CCCAACAGCAGAAGAAGTAATCACTGCGCTTGTACGAAACGAAGTGAGCTCGTACAAACAGCTACCGATCAACTTATACC
AAATCCAAACCAAATTCCGCGATGAGCGTCGTCCACGTTTTGGTGTGATGCGTGCACGTGAATTTTGCATGATGGATGCG
TACAGCTTCGATATCGACAAAGCTGGTCTGGAAAAATCTTACCAAGCAATGCACGATGCTTATTGCAAAGCGTTTGACCG
CATGGGTCTTGAATACCGCCCTGTTCTTGCAGATTCAGGTGCGATCGGTGGTAACGGTTCTCAGGAGTTCCACGTACTGG
CTGAAAGTGGTGAAGACCTGATCGCCTTCTCAACGGAATCAGATTACGCAGCCAACATCGAAAAAGCAGAAGCCGTTGCT
CCTGCGGTTGAGCGTGCAGCGCCAACTCAAGAGATGACGCTAGTGGATACGCCAAACGCTAAAACCATCGCTGAACTGGT
TGAACAGCACGGTATCTCAATCGAGAAAACCGTTAAAACGCTATTCGTTAAAGCGTCGGACGCAATTGAAGCGCCTATCG
TTGCACTGATCATCCGTGGTGATCACGAACTCAACGAAATCAAGGCAGAAAACCTTGCTGAAGTGGCAACGCCTTTAGAG
ATGGCAACAGAAGAAGAGATGCGTGAACTGATTGGCGCAGGTGCAGGTTCTCTTGGCCCTGTGGGCCTTAAACTACCATT
CATCGTTGACCGCTCGGTTGCTGTTATGAGCGATTTCGGTGCGGGCGCAAACATCGATGGCAAACACTACTTCGGTATCA
ACTGGGGTCGTGATGTAGAGCTTGGCCAAGTTGCTGACCTACGTAACGTCGTCGAAGGCGATCCAAGCCCATGTGGAAAA
GGCACATTGATGCTGAAACGTGGTATCGAAGTGGGTCACATTTTCCAACTCGGTAATGTGTACTCTGAAGCGATGAACTG
TGGCGTTCTCGATTCCAACGGTAAAAACGTGATCCTAGAGATGGGCTGTTACGGTATCGGTGTTTCTCGTGTGGTTGCTG
CGGCTATCGAACAAAACAACGACAAATACGGCATCATTTGGCCAGACGCACTTGCGCCTTTCCAAGTGGCTATCGTACCA
ATGAACATGCACAAATCTGAGCGCGTTCAAGAAGCTGCAGAGAAACTATACGCAGAGCTGACAGCGATGGGTATCGAAGT
GCTGTTCGATGACCGTAAAGAGCGCCCAGGCGTCATGTTCTCTGATATGGAACTGATCGGTATCCCTCACACTATCGTGA
TTGGCGATCGCAGCATGGACGAAGGTCACTTTGAGTATAAGAACCGTCGTTCTGGTGAAAAGACACCTGTCGCGATGGCG
GATATCGTTGAGTACATCAAAGCACAACTGGAATAA

Upstream 100 bases:

>100_bases
ACGCTTTTGACAATCACAAATGGCTGATATCATAGTCAGCTTAATTTTGTTCCCTCACTTTGAGGGGTTATTCAAACCAT
GATGAAACGGATACCATAGA

Downstream 100 bases:

>100_bases
CACCGAGTTCAGTGTTATAACAACAGTTAAAAGGGATTGGTCAAGGCCAATCCCTTTTCTATTAATCTTTGGTTCAGATT
TCTCCCTTTATGCTTACCTT

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 571; Mature: 571

Protein sequence:

>571_residues
MRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAIETLMPVVQPFELW
EETGRSEKMGPELLRFTDRHVRPFVLSPTAEEVITALVRNEVSSYKQLPINLYQIQTKFRDERRPRFGVMRAREFCMMDA
YSFDIDKAGLEKSYQAMHDAYCKAFDRMGLEYRPVLADSGAIGGNGSQEFHVLAESGEDLIAFSTESDYAANIEKAEAVA
PAVERAAPTQEMTLVDTPNAKTIAELVEQHGISIEKTVKTLFVKASDAIEAPIVALIIRGDHELNEIKAENLAEVATPLE
MATEEEMRELIGAGAGSLGPVGLKLPFIVDRSVAVMSDFGAGANIDGKHYFGINWGRDVELGQVADLRNVVEGDPSPCGK
GTLMLKRGIEVGHIFQLGNVYSEAMNCGVLDSNGKNVILEMGCYGIGVSRVVAAAIEQNNDKYGIIWPDALAPFQVAIVP
MNMHKSERVQEAAEKLYAELTAMGIEVLFDDRKERPGVMFSDMELIGIPHTIVIGDRSMDEGHFEYKNRRSGEKTPVAMA
DIVEYIKAQLE

Sequences:

>Translated_571_residues
MRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAIETLMPVVQPFELW
EETGRSEKMGPELLRFTDRHVRPFVLSPTAEEVITALVRNEVSSYKQLPINLYQIQTKFRDERRPRFGVMRAREFCMMDA
YSFDIDKAGLEKSYQAMHDAYCKAFDRMGLEYRPVLADSGAIGGNGSQEFHVLAESGEDLIAFSTESDYAANIEKAEAVA
PAVERAAPTQEMTLVDTPNAKTIAELVEQHGISIEKTVKTLFVKASDAIEAPIVALIIRGDHELNEIKAENLAEVATPLE
MATEEEMRELIGAGAGSLGPVGLKLPFIVDRSVAVMSDFGAGANIDGKHYFGINWGRDVELGQVADLRNVVEGDPSPCGK
GTLMLKRGIEVGHIFQLGNVYSEAMNCGVLDSNGKNVILEMGCYGIGVSRVVAAAIEQNNDKYGIIWPDALAPFQVAIVP
MNMHKSERVQEAAEKLYAELTAMGIEVLFDDRKERPGVMFSDMELIGIPHTIVIGDRSMDEGHFEYKNRRSGEKTPVAMA
DIVEYIKAQLE
>Mature_571_residues
MRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQEIDNAGAIETLMPVVQPFELW
EETGRSEKMGPELLRFTDRHVRPFVLSPTAEEVITALVRNEVSSYKQLPINLYQIQTKFRDERRPRFGVMRAREFCMMDA
YSFDIDKAGLEKSYQAMHDAYCKAFDRMGLEYRPVLADSGAIGGNGSQEFHVLAESGEDLIAFSTESDYAANIEKAEAVA
PAVERAAPTQEMTLVDTPNAKTIAELVEQHGISIEKTVKTLFVKASDAIEAPIVALIIRGDHELNEIKAENLAEVATPLE
MATEEEMRELIGAGAGSLGPVGLKLPFIVDRSVAVMSDFGAGANIDGKHYFGINWGRDVELGQVADLRNVVEGDPSPCGK
GTLMLKRGIEVGHIFQLGNVYSEAMNCGVLDSNGKNVILEMGCYGIGVSRVVAAAIEQNNDKYGIIWPDALAPFQVAIVP
MNMHKSERVQEAAEKLYAELTAMGIEVLFDDRKERPGVMFSDMELIGIPHTIVIGDRSMDEGHFEYKNRRSGEKTPVAMA
DIVEYIKAQLE

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=226, Percent_Identity=42.4778761061947, Blast_Score=197, Evalue=3e-50,
Organism=Escherichia coli, GI1786392, Length=571, Percent_Identity=71.2784588441331, Blast_Score=850, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115532348, Length=265, Percent_Identity=36.6037735849057, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI193203271, Length=99, Percent_Identity=38.3838383838384, Blast_Score=79, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320931, Length=544, Percent_Identity=31.4338235294118, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24656200, Length=228, Percent_Identity=34.2105263157895, Blast_Score=151, Evalue=1e-36,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 63164; Mature: 63164

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDNAGAIETLMPVVQPFELWEETGRSEKMGPELLRFTDRHVRPFVLSPTAEEVITALVRN
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EVSSYKQLPINLYQIQTKFRDERRPRFGVMRAREFCMMDAYSFDIDKAGLEKSYQAMHDA
HHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YCKAFDRMGLEYRPVLADSGAIGGNGSQEFHVLAESGEDLIAFSTESDYAANIEKAEAVA
HHHHHHHCCCEEECEEECCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHH
PAVERAAPTQEMTLVDTPNAKTIAELVEQHGISIEKTVKTLFVKASDAIEAPIVALIIRG
HHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCEEEEEEEC
DHELNEIKAENLAEVATPLEMATEEEMRELIGAGAGSLGPVGLKLPFIVDRSVAVMSDFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCEEECCCHHHHHCCC
AGANIDGKHYFGINWGRDVELGQVADLRNVVEGDPSPCGKGTLMLKRGIEVGHIFQLGNV
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHH
YSEAMNCGVLDSNGKNVILEMGCYGIGVSRVVAAAIEQNNDKYGIIWPDALAPFQVAIVP
HHHHHCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEE
MNMHKSERVQEAAEKLYAELTAMGIEVLFDDRKERPGVMFSDMELIGIPHTIVIGDRSMD
ECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEECCEEECCCEEEEECCCCCC
EGHFEYKNRRSGEKTPVAMADIVEYIKAQLE
CCCHHHHHCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRTSKYLLSTLKETPNDAEVVSHQLMLRAGMIRKLASGLYTWLPTGLRVLRKVENIVRQE
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDNAGAIETLMPVVQPFELWEETGRSEKMGPELLRFTDRHVRPFVLSPTAEEVITALVRN
HCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEECCCHHHHHHHHHHH
EVSSYKQLPINLYQIQTKFRDERRPRFGVMRAREFCMMDAYSFDIDKAGLEKSYQAMHDA
HHHHHHCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
YCKAFDRMGLEYRPVLADSGAIGGNGSQEFHVLAESGEDLIAFSTESDYAANIEKAEAVA
HHHHHHHCCCEEECEEECCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHHHH
PAVERAAPTQEMTLVDTPNAKTIAELVEQHGISIEKTVKTLFVKASDAIEAPIVALIIRG
HHHHHCCCCCCEEEEECCCHHHHHHHHHHHCCCHHHHHHHHHEECCCCCCCCEEEEEEEC
DHELNEIKAENLAEVATPLEMATEEEMRELIGAGAGSLGPVGLKLPFIVDRSVAVMSDFG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCEEECCCHHHHHCCC
AGANIDGKHYFGINWGRDVELGQVADLRNVVEGDPSPCGKGTLMLKRGIEVGHIFQLGNV
CCCCCCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHH
YSEAMNCGVLDSNGKNVILEMGCYGIGVSRVVAAAIEQNNDKYGIIWPDALAPFQVAIVP
HHHHHCCCCEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEE
MNMHKSERVQEAAEKLYAELTAMGIEVLFDDRKERPGVMFSDMELIGIPHTIVIGDRSMD
ECCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCEEECCEEECCCEEEEECCCCCC
EGHFEYKNRRSGEKTPVAMADIVEYIKAQLE
CCCHHHHHCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA