Click here to switch to the map view.

The map label for this gene is phoA [H]

Identifier: 37680582

GI number: 37680582

Start: 2430253

End: 2431830

Strand: Direct

Name: phoA [H]

Synonym: VV2398

Alternate gene names: 37680582

Gene position: 2430253-2431830 (Clockwise)

Preceding gene: 37680581

Following gene: 37680583

Centisome position: 72.45

GC content: 47.15

Gene sequence:

>1578_bases
ATGAAGCACCTGATCAAACCCGTTGTTGCCGTTGTAGCAACTTCTACGCTCTCTTTTAATGCACTTTCTGCAGAAATTAA
AAACGTCATTTTGATGATTGGTGATGGCATGGGGCCACAACAAGTTGGCCTATTGGAAACCTACGCCAACAACGCGCCAA
ATTCCATCTACCAAGGCAAAACCACCGCACTTTACACTCTCGCTCAAGAAGGCGTTATCGGCTCATCGCTGACACATCCA
GAAGACGCGATTGTGGTGGATTCAGCCTGTTCTGCAACCATGCTGGCGACCGGCATCATGACAGGTTCAGAAGTGATCGG
CATCGATTCGCAAGGGAACAGCGTCGAAACTGTACTCGAAAAAGCAAAACGTATGGGCAAAGCCACCGGCATCATTTCCG
ACACTCGCATGACGCACGCCACTCCTGCTGCCTTTGCCGCCCACCAGCCTCATCGCTCGTTGGAAAATGAAATTGCCGCT
GACATGCTTGCTACCGATGTCGATGTGCTGCTGTCTGGTGGTTTGCGTCATTGGATTCCAAAATCGGCAAACGACAAAGG
TGAAACCTATCAACAGTTACAAGCCCTAACTCAAGGTGACGTTGCATTAAAATCAAAACGCAAAGACGATCGCAACTTGC
TGACGGAAGCGCAACAAGCGGGTTACTCTCTGGCATTCAATAAAACCATGCTAGAGCAAGCGCAAGGGACGAAACTTTTG
GGTCTTTTTGCTAACTCAGCCATGTACGATGGCATCACCTACAGCCAAACCAAAGGTGACGCAGAGCGCAAAGAACCAAC
ACTGGCAGAAATGACCAGCAAAGCCTTGGATATCCTAGCCAAGGACAAAGACGGTTTCTTCTTGATGGTTGAAGGCGGAC
AAATCGACTGGGCTGGCCACAGCAATGATGCCGGTTCAATGCTGCATGAAATGATCAAGTTTGATGAAGCGATTAATACG
GTTTACCAATGGGCTAAAGATCGTGATGACACTTTGATTATTGTCACGGCGGACCACGAAACCGGCTCTTTTGGCTTTAG
CTACTCAGGCTATAATCTGCCAAAACCAGAGAAACGCCCAGGAAAAGCATTCGAAAATCGTGATTACGCGCCAAACTTTA
ACTTTGGTGCTTTCGATATTCTCGATGGTTTGTACAACCAAAAAACCAGCTACTATGGCATGCTTAGTGCGTTTGAAAAG
TTGGATAAAAATCAGCAAACCGCGGCAAAACTAGCAGAAATCATCAATAAAAATAGTGATTTTTCGATTACCGCAGGCCA
AGCTGAAAACATCCTGAAGAACAAACCCAATCCATATCGTTTGGCCAACCACAGCTATCTTGCTGAGGAAACCGTGCCAG
CCATTAACGACTTTGATGCGTTTTTCCCATACAACGACAGAACCAACCTGATCGCTCGTGAGCAAGCGACTAAGCAAAAT
ATCGTCTGGGGGACAGGCACACACACCCATACACCAGTGAATGTTTTTGCTTGGGGACCTGCAGAAACCATTCTCCCTGT
GTCACGCATTTTGCACCACTCTGAGTTGGGCGAGTTCATTAAAAATCAGGTGAAATAA

Upstream 100 bases:

>100_bases
TTTATTGATTTTTTCACTTTCATGAAGTTTTTGTGTCTGCAGCAAAAGTGTCACAAAGCAGTGCTGTAATGGCGCCGCTT
TTATAAATAAGGGATAGACA

Downstream 100 bases:

>100_bases
GCTCGGTTGATGAGCAGGTAAAGCCTTTGATTCAAATAAGAAAGTGCCAGATGGCGCTTTCTTATTTTGTTTTGCGCGCT
AATAAAGCGATTTTTCCAGA

Product: alkaline phosphatase

Products: NA

Alternate protein names: Alkaline phosphatase IV; APase IV [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MKHLIKPVVAVVATSTLSFNALSAEIKNVILMIGDGMGPQQVGLLETYANNAPNSIYQGKTTALYTLAQEGVIGSSLTHP
EDAIVVDSACSATMLATGIMTGSEVIGIDSQGNSVETVLEKAKRMGKATGIISDTRMTHATPAAFAAHQPHRSLENEIAA
DMLATDVDVLLSGGLRHWIPKSANDKGETYQQLQALTQGDVALKSKRKDDRNLLTEAQQAGYSLAFNKTMLEQAQGTKLL
GLFANSAMYDGITYSQTKGDAERKEPTLAEMTSKALDILAKDKDGFFLMVEGGQIDWAGHSNDAGSMLHEMIKFDEAINT
VYQWAKDRDDTLIIVTADHETGSFGFSYSGYNLPKPEKRPGKAFENRDYAPNFNFGAFDILDGLYNQKTSYYGMLSAFEK
LDKNQQTAAKLAEIINKNSDFSITAGQAENILKNKPNPYRLANHSYLAEETVPAINDFDAFFPYNDRTNLIAREQATKQN
IVWGTGTHTHTPVNVFAWGPAETILPVSRILHHSELGEFIKNQVK

Sequences:

>Translated_525_residues
MKHLIKPVVAVVATSTLSFNALSAEIKNVILMIGDGMGPQQVGLLETYANNAPNSIYQGKTTALYTLAQEGVIGSSLTHP
EDAIVVDSACSATMLATGIMTGSEVIGIDSQGNSVETVLEKAKRMGKATGIISDTRMTHATPAAFAAHQPHRSLENEIAA
DMLATDVDVLLSGGLRHWIPKSANDKGETYQQLQALTQGDVALKSKRKDDRNLLTEAQQAGYSLAFNKTMLEQAQGTKLL
GLFANSAMYDGITYSQTKGDAERKEPTLAEMTSKALDILAKDKDGFFLMVEGGQIDWAGHSNDAGSMLHEMIKFDEAINT
VYQWAKDRDDTLIIVTADHETGSFGFSYSGYNLPKPEKRPGKAFENRDYAPNFNFGAFDILDGLYNQKTSYYGMLSAFEK
LDKNQQTAAKLAEIINKNSDFSITAGQAENILKNKPNPYRLANHSYLAEETVPAINDFDAFFPYNDRTNLIAREQATKQN
IVWGTGTHTHTPVNVFAWGPAETILPVSRILHHSELGEFIKNQVK
>Mature_525_residues
MKHLIKPVVAVVATSTLSFNALSAEIKNVILMIGDGMGPQQVGLLETYANNAPNSIYQGKTTALYTLAQEGVIGSSLTHP
EDAIVVDSACSATMLATGIMTGSEVIGIDSQGNSVETVLEKAKRMGKATGIISDTRMTHATPAAFAAHQPHRSLENEIAA
DMLATDVDVLLSGGLRHWIPKSANDKGETYQQLQALTQGDVALKSKRKDDRNLLTEAQQAGYSLAFNKTMLEQAQGTKLL
GLFANSAMYDGITYSQTKGDAERKEPTLAEMTSKALDILAKDKDGFFLMVEGGQIDWAGHSNDAGSMLHEMIKFDEAINT
VYQWAKDRDDTLIIVTADHETGSFGFSYSGYNLPKPEKRPGKAFENRDYAPNFNFGAFDILDGLYNQKTSYYGMLSAFEK
LDKNQQTAAKLAEIINKNSDFSITAGQAENILKNKPNPYRLANHSYLAEETVPAINDFDAFFPYNDRTNLIAREQATKQN
IVWGTGTHTHTPVNVFAWGPAETILPVSRILHHSELGEFIKNQVK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=377, Percent_Identity=31.8302387267904, Blast_Score=139, Evalue=7e-33,
Organism=Homo sapiens, GI157266292, Length=363, Percent_Identity=31.6804407713499, Blast_Score=135, Evalue=9e-32,
Organism=Homo sapiens, GI94721246, Length=377, Percent_Identity=31.2997347480106, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI116734717, Length=362, Percent_Identity=30.3867403314917, Blast_Score=128, Evalue=1e-29,
Organism=Homo sapiens, GI294660770, Length=358, Percent_Identity=29.608938547486, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI294660772, Length=309, Percent_Identity=30.42071197411, Blast_Score=118, Evalue=1e-26,
Organism=Escherichia coli, GI48994877, Length=358, Percent_Identity=31.8435754189944, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6320689, Length=359, Percent_Identity=34.8189415041783, Blast_Score=163, Evalue=6e-41,
Organism=Drosophila melanogaster, GI21355981, Length=364, Percent_Identity=30.7692307692308, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI18859923, Length=341, Percent_Identity=32.5513196480938, Blast_Score=140, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24651554, Length=362, Percent_Identity=33.9779005524862, Blast_Score=136, Evalue=4e-32,
Organism=Drosophila melanogaster, GI21355151, Length=372, Percent_Identity=30.6451612903226, Blast_Score=135, Evalue=5e-32,
Organism=Drosophila melanogaster, GI21358067, Length=350, Percent_Identity=30.8571428571429, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24651556, Length=315, Percent_Identity=35.5555555555556, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24585213, Length=468, Percent_Identity=29.0598290598291, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24657827, Length=366, Percent_Identity=30.327868852459, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI19921912, Length=367, Percent_Identity=29.1553133514986, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI85725200, Length=370, Percent_Identity=28.6486486486486, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI85815833, Length=370, Percent_Identity=28.6486486486486, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21355149, Length=352, Percent_Identity=28.125, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24657835, Length=371, Percent_Identity=27.7628032345013, Blast_Score=103, Evalue=3e-22,
Organism=Drosophila melanogaster, GI24657842, Length=374, Percent_Identity=28.0748663101604, Blast_Score=103, Evalue=4e-22,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 57416; Mature: 57416

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHLIKPVVAVVATSTLSFNALSAEIKNVILMIGDGMGPQQVGLLETYANNAPNSIYQGK
CCHHHHHHHHHHHHHHCCHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHCCCCCCHHCCC
TTALYTLAQEGVIGSSLTHPEDAIVVDSACSATMLATGIMTGSEVIGIDSQGNSVETVLE
CCEEEEHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHH
KAKRMGKATGIISDTRMTHATPAAFAAHQPHRSLENEIAADMLATDVDVLLSGGLRHWIP
HHHHHHHHCCCEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
KSANDKGETYQQLQALTQGDVALKSKRKDDRNLLTEAQQAGYSLAFNKTMLEQAQGTKLL
CCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCEEEEHHHHHHHHCCCEEE
GLFANSAMYDGITYSQTKGDAERKEPTLAEMTSKALDILAKDKDGFFLMVEGGQIDWAGH
EEECCCHHHCCEEECCCCCCCHHCCCHHHHHHHHHHHHHEECCCCEEEEEECCEEEECCC
SNDAGSMLHEMIKFDEAINTVYQWAKDRDDTLIIVTADHETGSFGFSYSGYNLPKPEKRP
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCCCCCCCCC
GKAFENRDYAPNFNFGAFDILDGLYNQKTSYYGMLSAFEKLDKNQQTAAKLAEIINKNSD
CCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
FSITAGQAENILKNKPNPYRLANHSYLAEETVPAINDFDAFFPYNDRTNLIAREQATKQN
CEEECCCHHHHHHCCCCCEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHCCCC
IVWGTGTHTHTPVNVFAWGPAETILPVSRILHHSELGEFIKNQVK
CEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKHLIKPVVAVVATSTLSFNALSAEIKNVILMIGDGMGPQQVGLLETYANNAPNSIYQGK
CCHHHHHHHHHHHHHHCCHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHCCCCCCHHCCC
TTALYTLAQEGVIGSSLTHPEDAIVVDSACSATMLATGIMTGSEVIGIDSQGNSVETVLE
CCEEEEHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCEEEEECCCCCHHHHHHH
KAKRMGKATGIISDTRMTHATPAAFAAHQPHRSLENEIAADMLATDVDVLLSGGLRHWIP
HHHHHHHHCCCEECCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCC
KSANDKGETYQQLQALTQGDVALKSKRKDDRNLLTEAQQAGYSLAFNKTMLEQAQGTKLL
CCCCCCHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCEEEEHHHHHHHHCCCEEE
GLFANSAMYDGITYSQTKGDAERKEPTLAEMTSKALDILAKDKDGFFLMVEGGQIDWAGH
EEECCCHHHCCEEECCCCCCCHHCCCHHHHHHHHHHHHHEECCCCEEEEEECCEEEECCC
SNDAGSMLHEMIKFDEAINTVYQWAKDRDDTLIIVTADHETGSFGFSYSGYNLPKPEKRP
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEECCCCCCCCCCCC
GKAFENRDYAPNFNFGAFDILDGLYNQKTSYYGMLSAFEKLDKNQQTAAKLAEIINKNSD
CCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCC
FSITAGQAENILKNKPNPYRLANHSYLAEETVPAINDFDAFFPYNDRTNLIAREQATKQN
CEEECCCHHHHHHCCCCCEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCEEEHHHHCCCC
IVWGTGTHTHTPVNVFAWGPAETILPVSRILHHSELGEFIKNQVK
CEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113174; 9579061; 9384377; 1898729; 2125017; 2105301; 8144469 [H]