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The map label for this gene is xthA [H]

Identifier: 37680518

GI number: 37680518

Start: 2357661

End: 2358467

Strand: Direct

Name: xthA [H]

Synonym: VV2334

Alternate gene names: 37680518

Gene position: 2357661-2358467 (Clockwise)

Preceding gene: 37680516

Following gene: 37680525

Centisome position: 70.28

GC content: 46.72

Gene sequence:

>807_bases
ATGAAAATCGTCAGCTTCAACATCAATGGGTTACGAGCTCGCCTTCATCAACTGCAAGCATTGATCGACAAACATCAACC
TGATGTTATTGGTCTGCAAGAAATCAAAGTCCACGACGAAGCCTTTCCTATCGAAGACGTCGAAGCGATGGGCTATAAAG
TCTATTTCCACGGGCAAAAAGCGCATTACGGCGTGGCTCTGCTGTGTAAACAAGAGCCAATCGAAATCCAAAAAGGGTTC
CCAACCGATAATGACGAACATCAGAAGCGCATGATCATCGGGACTTTTCTTGATGAGAAGGGTGAAAAAGTGACGGTGTT
GAACGGCTATTTTCCGCAAGGGGACAACATAGCACACGAAACCAAGTTCCCTTATAAACGCCAGTTTTACCAAGATTTGA
TGGGATACCTAACCACTCACAGAGACAACGGTGAGCAAGTGGTTGTGATGGGCGACATCAATATCAGCCCGATCGACGCC
GACATCGGTATTGGTGAAGTGAACCGTAAACGCTGGTTAAAAACGGGCAAATGTTCGTTCCAGCCTGAGGAGCGTGAGTG
GTTACAAACCTTACTGGATTGGGGATTAGAAGATACTTTCCGCAAACTGCACCCAAGTGTGGATGATAAGTTCTCGTGGT
TTGACTACCGTTCAAAAGGCTTTGACGATAATCGTGGCCTGCGCATTGATGTGATTCTTGCTACACCATCGTTAGCGACC
AAATGCCGAGAATCCGATATCGATTACGAGCTACGCGGCATTGAGAAACCTTCAGATCACGCGCCTATTTGGGCAACCTT
TGAGTAA

Upstream 100 bases:

>100_bases
GAACCATCAATGTGATCAATCGCTGTGATTAGTTATCTCTTCTTGAAGTCATTGAGTTGAGTGGTAACATCAAGTCAAAC
AACACGAATCAGGATAAAGA

Downstream 100 bases:

>100_bases
CCCGTATTCGTTCATCGACATAAAGCAATCGACTTAAAATCATAGAGGAGCTAAGGCTCCTCTATGATTTTTTACTGCTG
ACTAGTTCGACGGCCAAGCG

Product: exonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MKIVSFNINGLRARLHQLQALIDKHQPDVIGLQEIKVHDEAFPIEDVEAMGYKVYFHGQKAHYGVALLCKQEPIEIQKGF
PTDNDEHQKRMIIGTFLDEKGEKVTVLNGYFPQGDNIAHETKFPYKRQFYQDLMGYLTTHRDNGEQVVVMGDINISPIDA
DIGIGEVNRKRWLKTGKCSFQPEEREWLQTLLDWGLEDTFRKLHPSVDDKFSWFDYRSKGFDDNRGLRIDVILATPSLAT
KCRESDIDYELRGIEKPSDHAPIWATFE

Sequences:

>Translated_268_residues
MKIVSFNINGLRARLHQLQALIDKHQPDVIGLQEIKVHDEAFPIEDVEAMGYKVYFHGQKAHYGVALLCKQEPIEIQKGF
PTDNDEHQKRMIIGTFLDEKGEKVTVLNGYFPQGDNIAHETKFPYKRQFYQDLMGYLTTHRDNGEQVVVMGDINISPIDA
DIGIGEVNRKRWLKTGKCSFQPEEREWLQTLLDWGLEDTFRKLHPSVDDKFSWFDYRSKGFDDNRGLRIDVILATPSLAT
KCRESDIDYELRGIEKPSDHAPIWATFE
>Mature_268_residues
MKIVSFNINGLRARLHQLQALIDKHQPDVIGLQEIKVHDEAFPIEDVEAMGYKVYFHGQKAHYGVALLCKQEPIEIQKGF
PTDNDEHQKRMIIGTFLDEKGEKVTVLNGYFPQGDNIAHETKFPYKRQFYQDLMGYLTTHRDNGEQVVVMGDINISPIDA
DIGIGEVNRKRWLKTGKCSFQPEEREWLQTLLDWGLEDTFRKLHPSVDDKFSWFDYRSKGFDDNRGLRIDVILATPSLAT
KCRESDIDYELRGIEKPSDHAPIWATFE

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=272, Percent_Identity=26.1029411764706, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI18375503, Length=272, Percent_Identity=26.1029411764706, Blast_Score=86, Evalue=4e-17,
Organism=Homo sapiens, GI18375501, Length=272, Percent_Identity=26.1029411764706, Blast_Score=86, Evalue=4e-17,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=64.0449438202247, Blast_Score=371, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI71989536, Length=266, Percent_Identity=26.6917293233083, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI221330655, Length=270, Percent_Identity=30.7407407407407, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI17136678, Length=266, Percent_Identity=30.8270676691729, Blast_Score=94, Evalue=7e-20,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 31027; Mature: 31027

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00726 AP_NUCLEASE_F1_1 ; PS00727 AP_NUCLEASE_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVSFNINGLRARLHQLQALIDKHQPDVIGLQEIKVHDEAFPIEDVEAMGYKVYFHGQK
CEEEEECCHHHHHHHHHHHHHHHHCCCCEECCEEEEECCCCCCCCCHHHCCEEEEEECCC
AHYGVALLCKQEPIEIQKGFPTDNDEHQKRMIIGTFLDEKGEKVTVLNGYFPQGDNIAHE
CCCCEEEEEECCCCEEECCCCCCCCCCHHEEEEEEEECCCCCEEEEEECCCCCCCCCCCC
TKFPYKRQFYQDLMGYLTTHRDNGEQVVVMGDINISPIDADIGIGEVNRKRWLKTGKCSF
CCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCC
QPEEREWLQTLLDWGLEDTFRKLHPSVDDKFSWFDYRSKGFDDNRGLRIDVILATPSLAT
CCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCHHHH
KCRESDIDYELRGIEKPSDHAPIWATFE
HHHCCCCCEEEECCCCCCCCCCEEEEEC
>Mature Secondary Structure
MKIVSFNINGLRARLHQLQALIDKHQPDVIGLQEIKVHDEAFPIEDVEAMGYKVYFHGQK
CEEEEECCHHHHHHHHHHHHHHHHCCCCEECCEEEEECCCCCCCCCHHHCCEEEEEECCC
AHYGVALLCKQEPIEIQKGFPTDNDEHQKRMIIGTFLDEKGEKVTVLNGYFPQGDNIAHE
CCCCEEEEEECCCCEEECCCCCCCCCCHHEEEEEEEECCCCCEEEEEECCCCCCCCCCCC
TKFPYKRQFYQDLMGYLTTHRDNGEQVVVMGDINISPIDADIGIGEVNRKRWLKTGKCSF
CCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCC
QPEEREWLQTLLDWGLEDTFRKLHPSVDDKFSWFDYRSKGFDDNRGLRIDVILATPSLAT
CCHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCHHHH
KCRESDIDYELRGIEKPSDHAPIWATFE
HHHCCCCCEEEECCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]