Click here to switch to the map view.

The map label for this gene is rluE

Identifier: 37680510

GI number: 37680510

Start: 2348778

End: 2349482

Strand: Direct

Name: rluE

Synonym: VV2326

Alternate gene names: 37680510

Gene position: 2348778-2349482 (Clockwise)

Preceding gene: 37680508

Following gene: 161486617

Centisome position: 70.02

GC content: 46.52

Gene sequence:

>705_bases
ATGTCATTCCGTTCTAGCAGCACTTCTCCACGCTCGAACAAGCAAACAGCGACCAAGCGATTTAAACAAAGCGGACAAGC
TCAATCTGTTGGCTCGCCAAAAAGACATAGTGTAAAGCGGAAGAACAATGAACAGAGCAAAAAATCTTTGTCGTTGGCAG
AGCGTAAAATCATTATCTTCAATAAGCCATATGACACGCTCAGCCAATTTACCGATGGTGAAGGTAGAAAAACGTTAGCA
GACTTTATTCCTGTCAAAGAAGTATACGCTGCGGGAAGATTAGATCGTGACAGTGAAGGCCTGATGGTGTTAACCAATGA
TGGCATACTGCAAGCCAAACTGACTCAGCCAAAATCCAAATCGCCTAAAACCTATTGGGTACAAGTAGAAGGCGCTCCGA
CCGAGGCACAACTCGAACCTCTTCGTCGTGGTGTTGAGCTGAAAGATGGGCTGACGTTACCCGCTCAAGTGGAGATTATC
CAAGATCCTCAGTTGTGGGAAAGAAATCCCCCTGTCCGATTTCGTGCTGCAATCCCCACCACTTGGCTAGCCATTACGAT
TATTGAAGGAAGAAATCGTCAAGTGCGACGCATGACGGCCCATATTGGCTTCCCGACTTTAAGGCTAGTACGTTACTCGA
TGGGAGGAATGACGTTACAAGATCTTCAACCCGGCGAGTGGAAGGAAATTTCCTTACCAAGTTAG

Upstream 100 bases:

>100_bases
GTGCGCTATTGTTAGTATAAGGCGCGCAGATCATATCCGATTCCGTCGAAAGTTAAAACTTATACACACACTTCATTTAC
ATTTTTGCAAGGTACTTAAC

Downstream 100 bases:

>100_bases
GGCTAATGCCCTAACTTAAGCAGTAACGTAAATACGATTACTCTTGATCCACCATCCACTTGCGGAAAGCTTTGCGCTCT
TCAGTCGATGCTTGTTTATA

Product: pseudouridine synthase family 1 protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E

Number of amino acids: Translated: 234; Mature: 233

Protein sequence:

>234_residues
MSFRSSSTSPRSNKQTATKRFKQSGQAQSVGSPKRHSVKRKNNEQSKKSLSLAERKIIIFNKPYDTLSQFTDGEGRKTLA
DFIPVKEVYAAGRLDRDSEGLMVLTNDGILQAKLTQPKSKSPKTYWVQVEGAPTEAQLEPLRRGVELKDGLTLPAQVEII
QDPQLWERNPPVRFRAAIPTTWLAITIIEGRNRQVRRMTAHIGFPTLRLVRYSMGGMTLQDLQPGEWKEISLPS

Sequences:

>Translated_234_residues
MSFRSSSTSPRSNKQTATKRFKQSGQAQSVGSPKRHSVKRKNNEQSKKSLSLAERKIIIFNKPYDTLSQFTDGEGRKTLA
DFIPVKEVYAAGRLDRDSEGLMVLTNDGILQAKLTQPKSKSPKTYWVQVEGAPTEAQLEPLRRGVELKDGLTLPAQVEII
QDPQLWERNPPVRFRAAIPTTWLAITIIEGRNRQVRRMTAHIGFPTLRLVRYSMGGMTLQDLQPGEWKEISLPS
>Mature_233_residues
SFRSSSTSPRSNKQTATKRFKQSGQAQSVGSPKRHSVKRKNNEQSKKSLSLAERKIIIFNKPYDTLSQFTDGEGRKTLAD
FIPVKEVYAAGRLDRDSEGLMVLTNDGILQAKLTQPKSKSPKTYWVQVEGAPTEAQLEPLRRGVELKDGLTLPAQVEIIQ
DPQLWERNPPVRFRAAIPTTWLAITIIEGRNRQVRRMTAHIGFPTLRLVRYSMGGMTLQDLQPGEWKEISLPS

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family

Homologues:

Organism=Escherichia coli, GI87081838, Length=202, Percent_Identity=60.3960396039604, Blast_Score=252, Evalue=1e-68,
Organism=Escherichia coli, GI1787524, Length=182, Percent_Identity=33.5164835164835, Blast_Score=88, Evalue=4e-19,
Organism=Escherichia coli, GI1788510, Length=182, Percent_Identity=34.0659340659341, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790453, Length=176, Percent_Identity=31.25, Blast_Score=84, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUE_VIBVU (Q8DAS3)

Other databases:

- EMBL:   AE016795
- RefSeq:   NP_760977.1
- ProteinModelPortal:   Q8DAS3
- SMR:   Q8DAS3
- GeneID:   1179004
- GenomeReviews:   AE016795_GR
- KEGG:   vvu:VV1_2116
- HOGENOM:   HBG754478
- OMA:   KHKLAKT
- ProtClustDB:   CLSK793972
- BioCyc:   VVUL216895:VV1_2116-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 26402; Mature: 26270

Theoretical pI: Translated: 10.90; Mature: 10.90

Prosite motif: PS01149 PSI_RSU

Important sites: ACT_SITE 95-95

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFRSSSTSPRSNKQTATKRFKQSGQAQSVGSPKRHSVKRKNNEQSKKSLSLAERKIIIF
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHEEEEEE
NKPYDTLSQFTDGEGRKTLADFIPVKEVYAAGRLDRDSEGLMVLTNDGILQAKLTQPKSK
ECCHHHHHHCCCCCCCHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCC
SPKTYWVQVEGAPTEAQLEPLRRGVELKDGLTLPAQVEIIQDPQLWERNPPVRFRAAIPT
CCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHCCCCCEEEEEECCC
TWLAITIIEGRNRQVRRMTAHIGFPTLRLVRYSMGGMTLQDLQPGEWKEISLPS
EEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
SFRSSSTSPRSNKQTATKRFKQSGQAQSVGSPKRHSVKRKNNEQSKKSLSLAERKIIIF
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHEEEEEE
NKPYDTLSQFTDGEGRKTLADFIPVKEVYAAGRLDRDSEGLMVLTNDGILQAKLTQPKSK
ECCHHHHHHCCCCCCCHHHHHHCCHHHHHHHCCCCCCCCCEEEEECCCEEEEEECCCCCC
SPKTYWVQVEGAPTEAQLEPLRRGVELKDGLTLPAQVEIIQDPQLWERNPPVRFRAAIPT
CCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCEEEEEECCCHHHCCCCCEEEEEECCC
TWLAITIIEGRNRQVRRMTAHIGFPTLRLVRYSMGGMTLQDLQPGEWKEISLPS
EEEEEEEEECCCCHHHHHHHHCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA