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The map label for this gene is dagA [H]

Identifier: 37680228

GI number: 37680228

Start: 2043994

End: 2045541

Strand: Direct

Name: dagA [H]

Synonym: VV2044

Alternate gene names: 37680228

Gene position: 2043994-2045541 (Clockwise)

Preceding gene: 37680216

Following gene: 37680229

Centisome position: 60.93

GC content: 49.03

Gene sequence:

>1548_bases
ATGGTAAAAAATGTAATGCTGGCCATGTTAGGCAGTACTGTGGCGTTCAACGCTTCAGCAGCCGGCTTGGATGAAAAAAT
CAACGAAGTCGTGGCACCTATCGTCAACCCCTTTGTTGGAATGATATTCTCGACCATTCCATTCCCTTTTTCTGACGTAC
AGGTTCCTTGGATTGTATTGTGGCTCGTCGTTGCTGCGACGTTTTTCACTTTTTATCTCGGCTTCATTAACGTACGCGGT
TTTAAACACGCGATTGATCTGGTTTCGGGCAAATACTCTGATCCTAAAGCCAAAGACCAAGGTGAAGTTTCTCACTTTCA
AGCCTTAACCACCGCCCTTTCCGGTACGGTTGGCCTTGGTAACATTGCCGGTGTTGCCGTCGCGGTCTCTATCGGGGGTG
CTGGTGCCACATTCTGGATGATACTGGCAGGCCTACTGGGCATGTCGAGTAAGTTTGTTGAATGTGCACTGGGCGTGAAA
TACCGCAATACCAACCCAGACGGCTCAGTGTCGGGTGGTCCTATGTACTACCTCAGCAAAGGTCTCGCCGATCGCGGTAA
CGCAGCGTTTGGTCGAACTCTTGCTGTGCTCTTCTCCGTATTTGCCATTGGTGGTGCTTTGGGTGGCGGTAACATGTTCC
AAGCCAACCAAGCGTTTAAGCAAGTGGTTGAAGTAACAGGCGGAGCATCTTCATTCCTTGCTGACAAAGGTTGGCTATTT
GGTTTGATCCTCGCCGCCATCGTCGGCGTCGTGATCATCGGTGGCATTAAATCGATCGCAAAAGTGACCGAGAAAGTGGT
GCCATTTATGGCGGGTATCTACGTTGGTACCGCATTGATCATTATCGCGATGAACTTTGACCGCATCGACGATGCATTCG
CAGCGATTTTCAATGGCGCCTTTACTGGCGAAGGCATCACTGGCGGTGTGATTGGTGTATTGATTCAGGGCTTTAAACGT
GCAGCATTCTCGAATGAAGCGGGTGTAGGCTCAGCGGCCATCGCACACGCGGCGGTAAAAACCAAAGAGCCGATGTCTGA
AGGTTTTGTTTCGCTCCTTGAACCCTTCATCGATACCGTCGTGATTTGTACTATGACCGCCTTGGTGATCATCATTACTG
GTTACTTGGATAACGGCTCTGGTCTCGCGGGTGTTGAACTGACGTCTGCCGCTTTCGGCAGTGCCATTAGCTGGTTCCCA
TACATCCTAGCGATCGCAGTGGTGCTGTTCGCGTTTTCAACGATGATTTCATGGTCATACTACGGCTTGAAAGCCTGGAC
TTACCTATTCGGTGAAAGCAAAACTGCAGAACTGATTTTCAAAATTAAATTCTGCATGTTTGTGGTGATTGGCTCTGCCA
TGAACCTAGGCCCAGTCATCGACTTCTCTGATGCGATGATCTTCGCCATGGCATTGGTGAACATTGTTGGCCTGTACATT
CTTGTTCCTGAAGTTAAACGCGATATGAAAGATTATCTAGCGCGCCTACAAGCAGGCAAAGTCTACAAAGTACAGCAGCA
AAATATCGAAGAACCGACACAAGCGTAA

Upstream 100 bases:

>100_bases
ATTGTTACCACTCAGAATAATGCACAATGTTACAAAAAGGTAACAACACAACATCAGATAATAATTATTCGTTTCTATTA
AAGAACAGACAGGATACACA

Downstream 100 bases:

>100_bases
CTGTCGGATACTCTCGTTAAAAAACAGCAACTTCGGTTGCTGTTTTTTGTCGATTAATCAAAATATGACGATCCACCAAC
ATCTTCTCCTCCAGTGGTTA

Product: sodium/alanine symporter

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 515; Mature: 515

Protein sequence:

>515_residues
MVKNVMLAMLGSTVAFNASAAGLDEKINEVVAPIVNPFVGMIFSTIPFPFSDVQVPWIVLWLVVAATFFTFYLGFINVRG
FKHAIDLVSGKYSDPKAKDQGEVSHFQALTTALSGTVGLGNIAGVAVAVSIGGAGATFWMILAGLLGMSSKFVECALGVK
YRNTNPDGSVSGGPMYYLSKGLADRGNAAFGRTLAVLFSVFAIGGALGGGNMFQANQAFKQVVEVTGGASSFLADKGWLF
GLILAAIVGVVIIGGIKSIAKVTEKVVPFMAGIYVGTALIIIAMNFDRIDDAFAAIFNGAFTGEGITGGVIGVLIQGFKR
AAFSNEAGVGSAAIAHAAVKTKEPMSEGFVSLLEPFIDTVVICTMTALVIIITGYLDNGSGLAGVELTSAAFGSAISWFP
YILAIAVVLFAFSTMISWSYYGLKAWTYLFGESKTAELIFKIKFCMFVVIGSAMNLGPVIDFSDAMIFAMALVNIVGLYI
LVPEVKRDMKDYLARLQAGKVYKVQQQNIEEPTQA

Sequences:

>Translated_515_residues
MVKNVMLAMLGSTVAFNASAAGLDEKINEVVAPIVNPFVGMIFSTIPFPFSDVQVPWIVLWLVVAATFFTFYLGFINVRG
FKHAIDLVSGKYSDPKAKDQGEVSHFQALTTALSGTVGLGNIAGVAVAVSIGGAGATFWMILAGLLGMSSKFVECALGVK
YRNTNPDGSVSGGPMYYLSKGLADRGNAAFGRTLAVLFSVFAIGGALGGGNMFQANQAFKQVVEVTGGASSFLADKGWLF
GLILAAIVGVVIIGGIKSIAKVTEKVVPFMAGIYVGTALIIIAMNFDRIDDAFAAIFNGAFTGEGITGGVIGVLIQGFKR
AAFSNEAGVGSAAIAHAAVKTKEPMSEGFVSLLEPFIDTVVICTMTALVIIITGYLDNGSGLAGVELTSAAFGSAISWFP
YILAIAVVLFAFSTMISWSYYGLKAWTYLFGESKTAELIFKIKFCMFVVIGSAMNLGPVIDFSDAMIFAMALVNIVGLYI
LVPEVKRDMKDYLARLQAGKVYKVQQQNIEEPTQA
>Mature_515_residues
MVKNVMLAMLGSTVAFNASAAGLDEKINEVVAPIVNPFVGMIFSTIPFPFSDVQVPWIVLWLVVAATFFTFYLGFINVRG
FKHAIDLVSGKYSDPKAKDQGEVSHFQALTTALSGTVGLGNIAGVAVAVSIGGAGATFWMILAGLLGMSSKFVECALGVK
YRNTNPDGSVSGGPMYYLSKGLADRGNAAFGRTLAVLFSVFAIGGALGGGNMFQANQAFKQVVEVTGGASSFLADKGWLF
GLILAAIVGVVIIGGIKSIAKVTEKVVPFMAGIYVGTALIIIAMNFDRIDDAFAAIFNGAFTGEGITGGVIGVLIQGFKR
AAFSNEAGVGSAAIAHAAVKTKEPMSEGFVSLLEPFIDTVVICTMTALVIIITGYLDNGSGLAGVELTSAAFGSAISWFP
YILAIAVVLFAFSTMISWSYYGLKAWTYLFGESKTAELIFKIKFCMFVVIGSAMNLGPVIDFSDAMIFAMALVNIVGLYI
LVPEVKRDMKDYLARLQAGKVYKVQQQNIEEPTQA

Specific function: Catalyzes the sodium-dependent uptake of extracellular D-alanine and glycine [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=447, Percent_Identity=32.2147651006711, Blast_Score=194, Evalue=9e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 54339; Mature: 54339

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKNVMLAMLGSTVAFNASAAGLDEKINEVVAPIVNPFVGMIFSTIPFPFSDVQVPWIVL
CCHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
WLVVAATFFTFYLGFINVRGFKHAIDLVSGKYSDPKAKDQGEVSHFQALTTALSGTVGLG
HHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
NIAGVAVAVSIGGAGATFWMILAGLLGMSSKFVECALGVKYRNTNPDGSVSGGPMYYLSK
HHHHHHEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCEEECCCCCCCCCCCCHHHHHH
GLADRGNAAFGRTLAVLFSVFAIGGALGGGNMFQANQAFKQVVEVTGGASSFLADKGWLF
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCHHHH
GLILAAIVGVVIIGGIKSIAKVTEKVVPFMAGIYVGTALIIIAMNFDRIDDAFAAIFNGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHCCC
FTGEGITGGVIGVLIQGFKRAAFSNEAGVGSAAIAHAAVKTKEPMSEGFVSLLEPFIDTV
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VICTMTALVIIITGYLDNGSGLAGVELTSAAFGSAISWFPYILAIAVVLFAFSTMISWSY
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGLKAWTYLFGESKTAELIFKIKFCMFVVIGSAMNLGPVIDFSDAMIFAMALVNIVGLYI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LVPEVKRDMKDYLARLQAGKVYKVQQQNIEEPTQA
HHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCCC
>Mature Secondary Structure
MVKNVMLAMLGSTVAFNASAAGLDEKINEVVAPIVNPFVGMIFSTIPFPFSDVQVPWIVL
CCHHHHHHHHCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
WLVVAATFFTFYLGFINVRGFKHAIDLVSGKYSDPKAKDQGEVSHFQALTTALSGTVGLG
HHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
NIAGVAVAVSIGGAGATFWMILAGLLGMSSKFVECALGVKYRNTNPDGSVSGGPMYYLSK
HHHHHHEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHCCEEECCCCCCCCCCCCHHHHHH
GLADRGNAAFGRTLAVLFSVFAIGGALGGGNMFQANQAFKQVVEVTGGASSFLADKGWLF
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHCCHHHH
GLILAAIVGVVIIGGIKSIAKVTEKVVPFMAGIYVGTALIIIAMNFDRIDDAFAAIFNGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHCCC
FTGEGITGGVIGVLIQGFKRAAFSNEAGVGSAAIAHAAVKTKEPMSEGFVSLLEPFIDTV
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
VICTMTALVIIITGYLDNGSGLAGVELTSAAFGSAISWFPYILAIAVVLFAFSTMISWSY
HHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGLKAWTYLFGESKTAELIFKIKFCMFVVIGSAMNLGPVIDFSDAMIFAMALVNIVGLYI
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LVPEVKRDMKDYLARLQAGKVYKVQQQNIEEPTQA
HHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447975 [H]