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The map label for this gene is truB

Identifier: 37523259

GI number: 37523259

Start: 3898481

End: 3899359

Strand: Reverse

Name: truB

Synonym: gll3690

Alternate gene names: 37523259

Gene position: 3899359-3898481 (Counterclockwise)

Preceding gene: 37523263

Following gene: 37523258

Centisome position: 83.69

GC content: 68.49

Gene sequence:

>879_bases
TTGGAAGGATTTCTCAACCTCGACAAACCCGCGGGGCTGACCTCCCACGACTGCATCGCCCGGCTGCGGCGGGTGCTGGG
CGAGCGGCGCATCGGTCACGGCGGCACCCTCGATCCGGCGGCCACCGGGGTATTGCCGGTGGCGGTGGGTCGCGCGACGC
GGCTGTTGCGCTTCTTGAGCGAAGGGAAGGTCTACCGGGCGACGGTGCGCTTTGGGCTGAGCACCGACACCGACGATCTC
GAAGGCAACATCCTCGCCGACGCCGGTGCGGCCGATCTCGATCTCGGCCGCGTACAGGTCCACCTGCAAGCTTTTCGCGG
CACGATCTCCCAGGTGCCGCCGCGCTACAGCGCCATTCACCAGGAGGGCGAACGGCTCTACGACCTGGCCCGCCGGGGGG
TGGCCATCGCCGAAATTGCCCCGCGCACCGTCGAAATCCAAGCGCTCACGGTGCTGGACTGGTATCCAGGCCACTACCCC
GAGTTGCAAATCGAGATCGCCTGCTCCACCGGCACCTACATCCGCTCGATCGCCCGCGATCTGGGGACGGCTCTCGGCAC
CGGTGCCACCCTCGCCCGCCTGCTGCGCACGCGAAGCGGGCCGTTTGCCCTCGAAGCGAGCCTGCCGCTGGCGGCGGTCG
AAGCGGGATTCCAGGCGGGCACCGTGCCGCTCATCGGACCGCGCACTGCCCTGGCTCACCTTGCCGCGGTGCATCTTGAA
CCACCGCTCGCGGCGCGCTGGTTGATGGGCCAGAGGGTGCCGGGCGATTGCGAGGGTGCTTTCGCACAGATCTGGGAGTG
CGAGACGGATCGCTTTTTGGGTGTGGCGGCTTGTGAGGCGGGTTCCCTGCAGCCGCTGGTGGTGCTGCCGACGCTTTGA

Upstream 100 bases:

>100_bases
TCCCCGTTGAGCAACCAAATTGTTCGCCGGGATCGAGCTGTAATGGGAAGTGTGGCCCATCGGTTCCGGTTCCATGGCAA
AGCGCTGCTCAGGCTCAAGT

Downstream 100 bases:

>100_bases
GCGCACAAGGCCGAGGGTTTGTTAACATTTGAAACGAACTGTTTGCCGGAGGCGAGCCGCCATGACCGCCGCCCAGGATT
CGCGAAGCCCGTATGCGTCG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 292; Mature: 292

Protein sequence:

>292_residues
MEGFLNLDKPAGLTSHDCIARLRRVLGERRIGHGGTLDPAATGVLPVAVGRATRLLRFLSEGKVYRATVRFGLSTDTDDL
EGNILADAGAADLDLGRVQVHLQAFRGTISQVPPRYSAIHQEGERLYDLARRGVAIAEIAPRTVEIQALTVLDWYPGHYP
ELQIEIACSTGTYIRSIARDLGTALGTGATLARLLRTRSGPFALEASLPLAAVEAGFQAGTVPLIGPRTALAHLAAVHLE
PPLAARWLMGQRVPGDCEGAFAQIWECETDRFLGVAACEAGSLQPLVVLPTL

Sequences:

>Translated_292_residues
MEGFLNLDKPAGLTSHDCIARLRRVLGERRIGHGGTLDPAATGVLPVAVGRATRLLRFLSEGKVYRATVRFGLSTDTDDL
EGNILADAGAADLDLGRVQVHLQAFRGTISQVPPRYSAIHQEGERLYDLARRGVAIAEIAPRTVEIQALTVLDWYPGHYP
ELQIEIACSTGTYIRSIARDLGTALGTGATLARLLRTRSGPFALEASLPLAAVEAGFQAGTVPLIGPRTALAHLAAVHLE
PPLAARWLMGQRVPGDCEGAFAQIWECETDRFLGVAACEAGSLQPLVVLPTL
>Mature_292_residues
MEGFLNLDKPAGLTSHDCIARLRRVLGERRIGHGGTLDPAATGVLPVAVGRATRLLRFLSEGKVYRATVRFGLSTDTDDL
EGNILADAGAADLDLGRVQVHLQAFRGTISQVPPRYSAIHQEGERLYDLARRGVAIAEIAPRTVEIQALTVLDWYPGHYP
ELQIEIACSTGTYIRSIARDLGTALGTGATLARLLRTRSGPFALEASLPLAAVEAGFQAGTVPLIGPRTALAHLAAVHLE
PPLAARWLMGQRVPGDCEGAFAQIWECETDRFLGVAACEAGSLQPLVVLPTL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=222, Percent_Identity=35.1351351351351, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI4503337, Length=202, Percent_Identity=33.1683168316832, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI215599015, Length=202, Percent_Identity=33.1683168316832, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI2367200, Length=294, Percent_Identity=32.9931972789116, Blast_Score=140, Evalue=7e-35,
Organism=Caenorhabditis elegans, GI17553978, Length=202, Percent_Identity=31.6831683168317, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=192, Percent_Identity=30.2083333333333, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=203, Percent_Identity=33.0049261083744, Blast_Score=88, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364189, Length=203, Percent_Identity=31.0344827586207, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364187, Length=203, Percent_Identity=31.0344827586207, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364185, Length=203, Percent_Identity=31.0344827586207, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281364183, Length=203, Percent_Identity=31.0344827586207, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI62471759, Length=203, Percent_Identity=31.0344827586207, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI17975520, Length=203, Percent_Identity=31.0344827586207, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_GLOVI (Q7MBB8)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_926636.1
- ProteinModelPortal:   Q7MBB8
- SMR:   Q7MBB8
- GeneID:   2602094
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll3690
- NMPDR:   fig|251221.1.peg.3690
- HOGENOM:   HBG397258
- OMA:   YPAHMSK
- ProtClustDB:   PRK02755
- BioCyc:   GVIO251221:GLL3690-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom

EC number: 4.2.1.70

Molecular weight: Translated: 31197; Mature: 31197

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: NA

Important sites: ACT_SITE 38-38

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGFLNLDKPAGLTSHDCIARLRRVLGERRIGHGGTLDPAATGVLPVAVGRATRLLRFLS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHH
EGKVYRATVRFGLSTDTDDLEGNILADAGAADLDLGRVQVHLQAFRGTISQVPPRYSAIH
CCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
QEGERLYDLARRGVAIAEIAPRTVEIQALTVLDWYPGHYPELQIEIACSTGTYIRSIARD
HHHHHHHHHHHCCEEEEECCCCEEEEEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHH
LGTALGTGATLARLLRTRSGPFALEASLPLAAVEAGFQAGTVPLIGPRTALAHLAAVHLE
HHHHHCCHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCEEEECCHHHHHHHHHHHCC
PPLAARWLMGQRVPGDCEGAFAQIWECETDRFLGVAACEAGSLQPLVVLPTL
CCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCEEEECCC
>Mature Secondary Structure
MEGFLNLDKPAGLTSHDCIARLRRVLGERRIGHGGTLDPAATGVLPVAVGRATRLLRFLS
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHH
EGKVYRATVRFGLSTDTDDLEGNILADAGAADLDLGRVQVHLQAFRGTISQVPPRYSAIH
CCCEEEEEEEECCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHH
QEGERLYDLARRGVAIAEIAPRTVEIQALTVLDWYPGHYPELQIEIACSTGTYIRSIARD
HHHHHHHHHHHCCEEEEECCCCEEEEEEEEEEECCCCCCCCEEEEEEECCCHHHHHHHHH
LGTALGTGATLARLLRTRSGPFALEASLPLAAVEAGFQAGTVPLIGPRTALAHLAAVHLE
HHHHHCCHHHHHHHHHHCCCCEEEECCCCHHHHHCCCCCCCEEEECCHHHHHHHHHHHCC
PPLAARWLMGQRVPGDCEGAFAQIWECETDRFLGVAACEAGSLQPLVVLPTL
CCHHHHHHHCCCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292