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The map label for this gene is fadD21 [H]

Identifier: 37521522

GI number: 37521522

Start: 2080190

End: 2081944

Strand: Reverse

Name: fadD21 [H]

Synonym: gll1953

Alternate gene names: 37521522

Gene position: 2081944-2080190 (Counterclockwise)

Preceding gene: 37521523

Following gene: 37521521

Centisome position: 44.69

GC content: 66.38

Gene sequence:

>1755_bases
ATGGTCAACCTACTCGACAATCCGTCCATTGCCGCCCAACCGACGACGCTCGTCGAAATGCTGCAGCGCCGGGCAATCCA
GCAGCCCGAAAGACTCGCCTTCACCTTCTTGGCCGACGGCGAGACGCAGGAGAGCAACCTCACCTACGGCGAACTCGATC
GGCGCGCCCGGGCGATCGCAGCCCATCTGCAGACTTTTGCCGCCGCCGGTGAACGGGCGGTGCTGCTCTATCCGACAGGA
TTGGACTACGTGGCCGCCTTCTACGGTTGCCTGTACGCAGGCGTGATCGCCGCACCGGCCTACCCACCCGATCCGACGCA
ACTGGAGAAGAGCCTCGCGGGTCTGCACGGGGTGATCCGCGACGCCCAGGCGCGCTGGGTGCTCACCACCACCGGCGTGC
ACGCGCTCGTCCACTCCCAACTGGCCGGCGGCGAAGAGCGGCAGCCGCTGCAGTGGCTGTGCACCGACCCGTTGCCCGAG
ACACCGGCCGAATCGTGGCGCCCCCCGCAGGTAGAGAGTGCTTCCATCGCGCTGCTGCAGTACACCTCGGGTTCCACAGC
CGCTCCGAAGGGCGTCATGCTCACCCACGCCAACGTCCTGCACAACCAGAAGCTGATCCAGAGCGCCTGCCACCACACGG
AGCAATCCACCTGGGTGACCTGGCTGCCTTTGCACCACAACCTGGCTTTGATGTCCGCGGTGGTGCAACCGGTCTACGTC
GGTTATCTGAGCGTGCTGATGCCACCGCCGGCTTTTTTGCAGCGGCCGTTGCGCTGGTTGAGGGCTATCTCGCGCTACCG
GGGCCGGGGGGCGGCAGGCCCCAACTTCGGCTTCAACCTCTGCATCAAAGAAATTCCGCCCGAACAGCGCGGCGAACTGG
ACCTGAGCAGTTGGGAGGTGGCGATTATCGGCGGTGAACCCATCCAGTGCGACTTGCTCGAGCGCTTTAGCGCCGCCTTC
GCCCCCTGCGGGTTTCGCCCCGAGACGTTCGTGCCCGGTTACGGCCTGGCGGAAAGCGTCCTGATGGTCTCCGTCGGCCG
CAAGTCCGAACGCCCAAAAGTCAAAGCCCTCGACAAGGCCCACCTGGCGGCGGGCCGCGCCGTCGATGCCCCCCCCGGCC
AGGCCGAGGCGATCAAAGTGGCCGGGTTCGAACTGTACGACCATGACCCGACCATCGCCGTCGTCCACCCCGAAACCCGG
CGGCGCTGTCAGCCGGGCGAGATCGGCGAAATCTGGGTGGCCGGCGGCAGTGTCGCAAGCGGCTACTGGCAAAAACCGGA
CATCTCCCAGGCGACCTTCGCAGCCCGGATCGCCGATTGCGCGGAAGGCCCGTTTTTGCGCACGGGAGATCTGGGCTTTA
TCGCCGACGGCCACTTTTTTCTGACCGGGCGCTGCAAAGAGCTCATCATCATCAGCGGCCGCAACCACTACCCGCAGGAC
ATCGAATTGACGGTGCAGACGAGCCATCCGGCCCTGCGGCCCAACTCCAGCGCCGCCTTTTCGATCCCATCGGACGGTGA
AGAGCGGCTGGTGGTGGCCGCGGAACTGGCCGACGACGATCCGGTGCCCGTGCCGGCGGCAAAGTCGATCGTGAGCGCCG
TCCAGCGGGCCGTCGCCGAGCAGCACGGTCTGCGGCCCACCGTGGTGCTGCTCAAACCCGGAACATTGCCCAAGACCGCC
ATCGGCAAAATCCAGCGCCTCGGGGCGCGGGCGCGCTACCTGGCCGGTACCCTCGAAGCCTGGAGCAGCCAATAG

Upstream 100 bases:

>100_bases
CGAGGCGGTGCTCGGGGCGGTCGCGGCGGGTTTGCTCGCCGCCCCGAGCGCCCACTAGCGGCGACCCCCACCCAAAATGT
CGTGATTTTAGAGCCAAACC

Downstream 100 bases:

>100_bases
GACCGTTTTCGCGCCATTTCACCAACGCAGGAACCACTTTGATGTTTGACCTTACCACTGCCCCCCCCGGCCGTCGGCAC
GCCGTCGTCATCGGCGGCAG

Product: acyl-CoA synthase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 584; Mature: 584

Protein sequence:

>584_residues
MVNLLDNPSIAAQPTTLVEMLQRRAIQQPERLAFTFLADGETQESNLTYGELDRRARAIAAHLQTFAAAGERAVLLYPTG
LDYVAAFYGCLYAGVIAAPAYPPDPTQLEKSLAGLHGVIRDAQARWVLTTTGVHALVHSQLAGGEERQPLQWLCTDPLPE
TPAESWRPPQVESASIALLQYTSGSTAAPKGVMLTHANVLHNQKLIQSACHHTEQSTWVTWLPLHHNLALMSAVVQPVYV
GYLSVLMPPPAFLQRPLRWLRAISRYRGRGAAGPNFGFNLCIKEIPPEQRGELDLSSWEVAIIGGEPIQCDLLERFSAAF
APCGFRPETFVPGYGLAESVLMVSVGRKSERPKVKALDKAHLAAGRAVDAPPGQAEAIKVAGFELYDHDPTIAVVHPETR
RRCQPGEIGEIWVAGGSVASGYWQKPDISQATFAARIADCAEGPFLRTGDLGFIADGHFFLTGRCKELIIISGRNHYPQD
IELTVQTSHPALRPNSSAAFSIPSDGEERLVVAAELADDDPVPVPAAKSIVSAVQRAVAEQHGLRPTVVLLKPGTLPKTA
IGKIQRLGARARYLAGTLEAWSSQ

Sequences:

>Translated_584_residues
MVNLLDNPSIAAQPTTLVEMLQRRAIQQPERLAFTFLADGETQESNLTYGELDRRARAIAAHLQTFAAAGERAVLLYPTG
LDYVAAFYGCLYAGVIAAPAYPPDPTQLEKSLAGLHGVIRDAQARWVLTTTGVHALVHSQLAGGEERQPLQWLCTDPLPE
TPAESWRPPQVESASIALLQYTSGSTAAPKGVMLTHANVLHNQKLIQSACHHTEQSTWVTWLPLHHNLALMSAVVQPVYV
GYLSVLMPPPAFLQRPLRWLRAISRYRGRGAAGPNFGFNLCIKEIPPEQRGELDLSSWEVAIIGGEPIQCDLLERFSAAF
APCGFRPETFVPGYGLAESVLMVSVGRKSERPKVKALDKAHLAAGRAVDAPPGQAEAIKVAGFELYDHDPTIAVVHPETR
RRCQPGEIGEIWVAGGSVASGYWQKPDISQATFAARIADCAEGPFLRTGDLGFIADGHFFLTGRCKELIIISGRNHYPQD
IELTVQTSHPALRPNSSAAFSIPSDGEERLVVAAELADDDPVPVPAAKSIVSAVQRAVAEQHGLRPTVVLLKPGTLPKTA
IGKIQRLGARARYLAGTLEAWSSQ
>Mature_584_residues
MVNLLDNPSIAAQPTTLVEMLQRRAIQQPERLAFTFLADGETQESNLTYGELDRRARAIAAHLQTFAAAGERAVLLYPTG
LDYVAAFYGCLYAGVIAAPAYPPDPTQLEKSLAGLHGVIRDAQARWVLTTTGVHALVHSQLAGGEERQPLQWLCTDPLPE
TPAESWRPPQVESASIALLQYTSGSTAAPKGVMLTHANVLHNQKLIQSACHHTEQSTWVTWLPLHHNLALMSAVVQPVYV
GYLSVLMPPPAFLQRPLRWLRAISRYRGRGAAGPNFGFNLCIKEIPPEQRGELDLSSWEVAIIGGEPIQCDLLERFSAAF
APCGFRPETFVPGYGLAESVLMVSVGRKSERPKVKALDKAHLAAGRAVDAPPGQAEAIKVAGFELYDHDPTIAVVHPETR
RRCQPGEIGEIWVAGGSVASGYWQKPDISQATFAARIADCAEGPFLRTGDLGFIADGHFFLTGRCKELIIISGRNHYPQD
IELTVQTSHPALRPNSSAAFSIPSDGEERLVVAAELADDDPVPVPAAKSIVSAVQRAVAEQHGLRPTVVLLKPGTLPKTA
IGKIQRLGARARYLAGTLEAWSSQ

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI44888818, Length=594, Percent_Identity=26.5993265993266, Blast_Score=151, Evalue=2e-36,
Organism=Homo sapiens, GI55749758, Length=591, Percent_Identity=26.2267343485618, Blast_Score=147, Evalue=4e-35,
Organism=Homo sapiens, GI45827692, Length=590, Percent_Identity=26.271186440678, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI225735629, Length=590, Percent_Identity=26.271186440678, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI225735625, Length=604, Percent_Identity=25.6622516556291, Blast_Score=132, Evalue=9e-31,
Organism=Homo sapiens, GI45827694, Length=544, Percent_Identity=25.3676470588235, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI225735627, Length=513, Percent_Identity=25.3411306042885, Blast_Score=116, Evalue=5e-26,
Organism=Homo sapiens, GI45827696, Length=513, Percent_Identity=25.3411306042885, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI45827698, Length=477, Percent_Identity=25.3668763102725, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1788107, Length=587, Percent_Identity=25.8943781942078, Blast_Score=132, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI193202679, Length=626, Percent_Identity=23.482428115016, Blast_Score=137, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24654656, Length=630, Percent_Identity=24.6031746031746, Blast_Score=129, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 63243; Mature: 63243

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNLLDNPSIAAQPTTLVEMLQRRAIQQPERLAFTFLADGETQESNLTYGELDRRARAIA
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHH
AHLQTFAAAGERAVLLYPTGLDYVAAFYGCLYAGVIAAPAYPPDPTQLEKSLAGLHGVIR
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DAQARWVLTTTGVHALVHSQLAGGEERQPLQWLCTDPLPETPAESWRPPQVESASIALLQ
CCCCCEEEEECHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEE
YTSGSTAAPKGVMLTHANVLHNQKLIQSACHHTEQSTWVTWLPLHHNLALMSAVVQPVYV
ECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHH
GYLSVLMPPPAFLQRPLRWLRAISRYRGRGAAGPNFGFNLCIKEIPPEQRGELDLSSWEV
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEE
AIIGGEPIQCDLLERFSAAFAPCGFRPETFVPGYGLAESVLMVSVGRKSERPKVKALDKA
EEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHH
HLAAGRAVDAPPGQAEAIKVAGFELYDHDPTIAVVHPETRRRCQPGEIGEIWVAGGSVAS
HHHCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCHHHCCCCCCCCCEEEEECCCCCC
GYWQKPDISQATFAARIADCAEGPFLRTGDLGFIADGHFFLTGRCKELIIISGRNHYPQD
CCCCCCCCCHHHHHHHHHHHCCCCEEEECCEEEEECCCEEEECCCEEEEEEECCCCCCCC
IELTVQTSHPALRPNSSAAFSIPSDGEERLVVAAELADDDPVPVPAAKSIVSAVQRAVAE
EEEEEECCCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
QHGLRPTVVLLKPGTLPKTAIGKIQRLGARARYLAGTLEAWSSQ
HHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVNLLDNPSIAAQPTTLVEMLQRRAIQQPERLAFTFLADGETQESNLTYGELDRRARAIA
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHH
AHLQTFAAAGERAVLLYPTGLDYVAAFYGCLYAGVIAAPAYPPDPTQLEKSLAGLHGVIR
HHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
DAQARWVLTTTGVHALVHSQLAGGEERQPLQWLCTDPLPETPAESWRPPQVESASIALLQ
CCCCCEEEEECHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEEEEEE
YTSGSTAAPKGVMLTHANVLHNQKLIQSACHHTEQSTWVTWLPLHHNLALMSAVVQPVYV
ECCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHH
GYLSVLMPPPAFLQRPLRWLRAISRYRGRGAAGPNFGFNLCIKEIPPEQRGELDLSSWEV
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEE
AIIGGEPIQCDLLERFSAAFAPCGFRPETFVPGYGLAESVLMVSVGRKSERPKVKALDKA
EEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHH
HLAAGRAVDAPPGQAEAIKVAGFELYDHDPTIAVVHPETRRRCQPGEIGEIWVAGGSVAS
HHHCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCHHHCCCCCCCCCEEEEECCCCCC
GYWQKPDISQATFAARIADCAEGPFLRTGDLGFIADGHFFLTGRCKELIIISGRNHYPQD
CCCCCCCCCHHHHHHHHHHHCCCCEEEECCEEEEECCCEEEECCCEEEEEEECCCCCCCC
IELTVQTSHPALRPNSSAAFSIPSDGEERLVVAAELADDDPVPVPAAKSIVSAVQRAVAE
EEEEEECCCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHH
QHGLRPTVVLLKPGTLPKTAIGKIQRLGARARYLAGTLEAWSSQ
HHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]