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The map label for this gene is fadD21 [H]

Identifier: 37521517

GI number: 37521517

Start: 2073530

End: 2075320

Strand: Reverse

Name: fadD21 [H]

Synonym: gll1948

Alternate gene names: 37521517

Gene position: 2075320-2073530 (Counterclockwise)

Preceding gene: 37521518

Following gene: 37521516

Centisome position: 44.54

GC content: 66.39

Gene sequence:

>1791_bases
ATGACAAAAAGCTCAGGAAAACCACAGATGGCGACAGCAACCCGGACGGCCGACAACCTGGTCGAGGCCCTCAGGCGGTG
GGCTTCCGTGCGCTCCGCCCGGAACGCTTACACCTGGGAAGGGGAGAGCGCCGGAGCTGTGCGACTGACGTTCTTTGAAA
TCGATCGCCTGGCCAGGGCGATCGCCGCCAGGCTGCAGCAGTCGAACTTGCGCGGACGACGGGCGGTGCTGCTCTATCCG
CCGGGGCTGGAATTTGTGGCGGCCTTTATGGGGTGCCTGTACGCCGGTGTAACGGCAGTTCCGGCCCACTCCCCGCGGCC
GCGCCGCCCGGCGCCGAAGCTCCAGGCCATCCTGGCGGACGCTGGGGTGACGGCGGTGCTGACGACCGCAGCTTCCCTGG
CCCGCAGCGATCAGTGGACCACCCAGAATCCCCAACTGGCCGGGTTGCAATGGCTGGCCACCGACAACCTCGGCACCGAT
CTGGCCGACACCTGGCAGGAGTGCACTTTCTCCAAGGACAGCCTCGCGTTTTTGCAGTACACCTCCGGATCGACAGGCCA
TCCGAAGGGGGTAATGATCACCCACGGCAACTTGCTGCACAACCAGCGGATGGTCGAAGGCGCCTTCGGGCACAGCGACG
AGACGATCTTCGCGGGCTGGCTGCCGCTCTTTCACGACATGGGACTAATCGGCAACGTGCTGCAGCCGCTCCACCTGGGT
ATCCCCTGTGTCTTGATGTCACCGGTGGATTTTGTGCAGAAGCCGCGCCGCTGGCTCGAAGCGATCACCCGCCACCGCGC
CACCACCAGCGGCGGGCCGAACTTTGCCTACGACCTGTGCGTGCGCAAGGTGTCGGCTGAGCAGCGCGAGGGGCTCGATC
TGAGCAGTTGGCGCGTGGCCTTCAACGGAGCGGAGCCGGTGCGCGCCCACACCCTCCAGGCGTTTGCTGCCGCCTTCGCC
GGGTGTGGATTTCGGGCGAAGGCGTTCTATCCTTGCTACGGCCTGGCGGAGGCCACCCTGTTTGTGACAGGTGGTCTTTT
TCAGACCCCCGCCGTCGTGCGCAGCGTCGCCGCCGCCGCCCTTGAGCAAGACAGGGTCGTGCCCGCCGCCGAGGACGATC
CCGGCAGCCGCCTGCTGGTGGGTTGCGGACGACCCTGGTTGGGGCAGGAGGTGGTGATCGCCGATCCGACGAATTTGACG
CTCTGCCCCCCCGCGCAGGTGGGTGAAATCTGGGTTGCAGGGCCGAGTGTCGCCCGGGGCTACTGGAACCGGCCCGCCGA
AACCGCAGCGACTTTCGGCGCCCGTCTGGCCGGCACGGGTGCCGGGCCATTCCTGCGCACGGGCGATCTCGGGTTTATCG
AGGGCGGGGAGTTGTTCGTCACCGGCCGCCTCAAGGACTTGATCATCATTCGGGGGGCGAACCATTACCCCCAGGACATC
GAAGCATCGGTGGAAAATTGTCACCGGGCCTTGCGCCCGGGCTGTGGGGCGGCTTTTGCCGTCGAATTCGACGGCGAAGA
ACGGCTGGTGGTCGTCCAGGAACTCGAACGACAGCTTCCCCAAGCGCTGGATTTCGAAGAAGTGCTCAGCAGTATCCGCC
AGGCGGTGGCGGCCCGCCACGGGCTGCACGTCGATACGGCCGCGCTGATCAAAACCGGCTCCATCCCCAAGACCTCCAGC
GGCAAAATCCAGCGCCGCACCTGCCGTAGCCAGTTTCTCGACGGGACGCTGCCGGTGCTCTACGCCGGGGGCAGAAGCCC
GATGCCGCCTGCGGGGCGCCCGAGTGTCTGA

Upstream 100 bases:

>100_bases
TGCCGATAGCGGGAATCTTCCCGCTGCGGGACGTCCGCGAAGCCCACGCGCGGATGGAGCGGGGCGCACACATCGGCAAG
TTCATCCTGGTGCCCTGAAG

Downstream 100 bases:

>100_bases
ACATTTCACAGGAAGCCGAACGATGTCATCGCCCTTCGAGCGCGCCATGCAGAGCAATCCGCCTGCCAGTCCGCTCCCCT
GGCAAAAGACGATCATCAAA

Product: acyl-CoA synthase

Products: AMP; diphosphate +an acyl-CoA [C]

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MTKSSGKPQMATATRTADNLVEALRRWASVRSARNAYTWEGESAGAVRLTFFEIDRLARAIAARLQQSNLRGRRAVLLYP
PGLEFVAAFMGCLYAGVTAVPAHSPRPRRPAPKLQAILADAGVTAVLTTAASLARSDQWTTQNPQLAGLQWLATDNLGTD
LADTWQECTFSKDSLAFLQYTSGSTGHPKGVMITHGNLLHNQRMVEGAFGHSDETIFAGWLPLFHDMGLIGNVLQPLHLG
IPCVLMSPVDFVQKPRRWLEAITRHRATTSGGPNFAYDLCVRKVSAEQREGLDLSSWRVAFNGAEPVRAHTLQAFAAAFA
GCGFRAKAFYPCYGLAEATLFVTGGLFQTPAVVRSVAAAALEQDRVVPAAEDDPGSRLLVGCGRPWLGQEVVIADPTNLT
LCPPAQVGEIWVAGPSVARGYWNRPAETAATFGARLAGTGAGPFLRTGDLGFIEGGELFVTGRLKDLIIIRGANHYPQDI
EASVENCHRALRPGCGAAFAVEFDGEERLVVVQELERQLPQALDFEEVLSSIRQAVAARHGLHVDTAALIKTGSIPKTSS
GKIQRRTCRSQFLDGTLPVLYAGGRSPMPPAGRPSV

Sequences:

>Translated_596_residues
MTKSSGKPQMATATRTADNLVEALRRWASVRSARNAYTWEGESAGAVRLTFFEIDRLARAIAARLQQSNLRGRRAVLLYP
PGLEFVAAFMGCLYAGVTAVPAHSPRPRRPAPKLQAILADAGVTAVLTTAASLARSDQWTTQNPQLAGLQWLATDNLGTD
LADTWQECTFSKDSLAFLQYTSGSTGHPKGVMITHGNLLHNQRMVEGAFGHSDETIFAGWLPLFHDMGLIGNVLQPLHLG
IPCVLMSPVDFVQKPRRWLEAITRHRATTSGGPNFAYDLCVRKVSAEQREGLDLSSWRVAFNGAEPVRAHTLQAFAAAFA
GCGFRAKAFYPCYGLAEATLFVTGGLFQTPAVVRSVAAAALEQDRVVPAAEDDPGSRLLVGCGRPWLGQEVVIADPTNLT
LCPPAQVGEIWVAGPSVARGYWNRPAETAATFGARLAGTGAGPFLRTGDLGFIEGGELFVTGRLKDLIIIRGANHYPQDI
EASVENCHRALRPGCGAAFAVEFDGEERLVVVQELERQLPQALDFEEVLSSIRQAVAARHGLHVDTAALIKTGSIPKTSS
GKIQRRTCRSQFLDGTLPVLYAGGRSPMPPAGRPSV
>Mature_595_residues
TKSSGKPQMATATRTADNLVEALRRWASVRSARNAYTWEGESAGAVRLTFFEIDRLARAIAARLQQSNLRGRRAVLLYPP
GLEFVAAFMGCLYAGVTAVPAHSPRPRRPAPKLQAILADAGVTAVLTTAASLARSDQWTTQNPQLAGLQWLATDNLGTDL
ADTWQECTFSKDSLAFLQYTSGSTGHPKGVMITHGNLLHNQRMVEGAFGHSDETIFAGWLPLFHDMGLIGNVLQPLHLGI
PCVLMSPVDFVQKPRRWLEAITRHRATTSGGPNFAYDLCVRKVSAEQREGLDLSSWRVAFNGAEPVRAHTLQAFAAAFAG
CGFRAKAFYPCYGLAEATLFVTGGLFQTPAVVRSVAAAALEQDRVVPAAEDDPGSRLLVGCGRPWLGQEVVIADPTNLTL
CPPAQVGEIWVAGPSVARGYWNRPAETAATFGARLAGTGAGPFLRTGDLGFIEGGELFVTGRLKDLIIIRGANHYPQDIE
ASVENCHRALRPGCGAAFAVEFDGEERLVVVQELERQLPQALDFEEVLSSIRQAVAARHGLHVDTAALIKTGSIPKTSSG
KIQRRTCRSQFLDGTLPVLYAGGRSPMPPAGRPSV

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI225735629, Length=556, Percent_Identity=28.4172661870504, Blast_Score=147, Evalue=4e-35,
Organism=Homo sapiens, GI45827692, Length=556, Percent_Identity=28.4172661870504, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI44888818, Length=568, Percent_Identity=26.7605633802817, Blast_Score=143, Evalue=5e-34,
Organism=Homo sapiens, GI55749758, Length=539, Percent_Identity=28.7569573283859, Blast_Score=140, Evalue=4e-33,
Organism=Homo sapiens, GI225735625, Length=627, Percent_Identity=27.1132376395534, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI45827694, Length=562, Percent_Identity=26.8683274021352, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI45827696, Length=528, Percent_Identity=26.5151515151515, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI225735627, Length=528, Percent_Identity=26.5151515151515, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI45827698, Length=497, Percent_Identity=26.7605633802817, Blast_Score=86, Evalue=9e-17,
Organism=Escherichia coli, GI145693145, Length=402, Percent_Identity=26.1194029850746, Blast_Score=104, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI193202679, Length=574, Percent_Identity=25.2613240418118, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17531443, Length=420, Percent_Identity=23.3333333333333, Blast_Score=92, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6319699, Length=405, Percent_Identity=24.4444444444444, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24654656, Length=575, Percent_Identity=26.4347826086956, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI18859661, Length=581, Percent_Identity=24.4406196213425, Blast_Score=119, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.2.1.3 [C]

Molecular weight: Translated: 64140; Mature: 64009

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSSGKPQMATATRTADNLVEALRRWASVRSARNAYTWEGESAGAVRLTFFEIDRLARA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEHHHHHHHHHH
IAARLQQSNLRGRRAVLLYPPGLEFVAAFMGCLYAGVTAVPAHSPRPRRPAPKLQAILAD
HHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHH
AGVTAVLTTAASLARSDQWTTQNPQLAGLQWLATDNLGTDLADTWQECTFSKDSLAFLQY
CCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEE
TSGSTGHPKGVMITHGNLLHNQRMVEGAFGHSDETIFAGWLPLFHDMGLIGNVLQPLHLG
CCCCCCCCCEEEEECCCHHHCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCC
IPCVLMSPVDFVQKPRRWLEAITRHRATTSGGPNFAYDLCVRKVSAEQREGLDLSSWRVA
CCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
FNGAEPVRAHTLQAFAAAFAGCGFRAKAFYPCYGLAEATLFVTGGLFQTPAVVRSVAAAA
ECCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHEEEEEECCCHHCHHHHHHHHHHH
LEQDRVVPAAEDDPGSRLLVGCGRPWLGQEVVIADPTNLTLCPPAQVGEIWVAGPSVARG
HHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCEEECCCCCCCCEEEECCHHHHH
YWNRPAETAATFGARLAGTGAGPFLRTGDLGFIEGGELFVTGRLKDLIIIRGANHYPQDI
CCCCCHHHHHHHHHHHCCCCCCCCEECCCCCEECCCCEEEEECCEEEEEEECCCCCCHHH
EASVENCHRALRPGCGAAFAVEFDGEERLVVVQELERQLPQALDFEEVLSSIRQAVAARH
HHHHHHHHHHHCCCCCEEEEEEECCCCCEEHHHHHHHHCHHCCCHHHHHHHHHHHHHHHC
GLHVDTAALIKTGSIPKTSSGKIQRRTCRSQFLDGTLPVLYAGGRSPMPPAGRPSV
CCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TKSSGKPQMATATRTADNLVEALRRWASVRSARNAYTWEGESAGAVRLTFFEIDRLARA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEHHHHHHHHHH
IAARLQQSNLRGRRAVLLYPPGLEFVAAFMGCLYAGVTAVPAHSPRPRRPAPKLQAILAD
HHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCEECCCCCCCCCCCCHHHHHHHHH
AGVTAVLTTAASLARSDQWTTQNPQLAGLQWLATDNLGTDLADTWQECTFSKDSLAFLQY
CCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCEEEEEE
TSGSTGHPKGVMITHGNLLHNQRMVEGAFGHSDETIFAGWLPLFHDMGLIGNVLQPLHLG
CCCCCCCCCEEEEECCCHHHCCHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCC
IPCVLMSPVDFVQKPRRWLEAITRHRATTSGGPNFAYDLCVRKVSAEQREGLDLSSWRVA
CCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEE
FNGAEPVRAHTLQAFAAAFAGCGFRAKAFYPCYGLAEATLFVTGGLFQTPAVVRSVAAAA
ECCCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHEEEEEECCCHHCHHHHHHHHHHH
LEQDRVVPAAEDDPGSRLLVGCGRPWLGQEVVIADPTNLTLCPPAQVGEIWVAGPSVARG
HHHCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCEEECCCCCCCCEEEECCHHHHH
YWNRPAETAATFGARLAGTGAGPFLRTGDLGFIEGGELFVTGRLKDLIIIRGANHYPQDI
CCCCCHHHHHHHHHHHCCCCCCCCEECCCCCEECCCCEEEEECCEEEEEEECCCCCCHHH
EASVENCHRALRPGCGAAFAVEFDGEERLVVVQELERQLPQALDFEEVLSSIRQAVAARH
HHHHHHHHHHHCCCCCEEEEEEECCCCCEEHHHHHHHHCHHCCCHHHHHHHHHHHHHHHC
GLHVDTAALIKTGSIPKTSSGKIQRRTCRSQFLDGTLPVLYAGGRSPMPPAGRPSV
CCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Salts [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0408 {octanoate}} 0.0125 {octanoate}} 0.0059 {octanoate}} 0.00072 {octanoate}} 0.0013 {undecanoate}} 0.0022 {pentadecanoate}} 0.053 {CoA}} 0.035 {CoA}} 0.034 {CoA}} 0.0034 {tridecanoate}} 0.002 {laurate}} 0.0019 {laurate}} 0.0

Substrates: ATP; a long-chain carboxylic acid; CoA [C]

Specific reaction: ATP + a long-chain carboxylic acid + CoA = AMP + diphosphate +an acyl-CoA [C]

General reaction: Acid-thiol ligation; Phosphorylation [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]