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The map label for this gene is thiG

Identifier: 37521376

GI number: 37521376

Start: 1925961

End: 1926785

Strand: Reverse

Name: thiG

Synonym: gvip247

Alternate gene names: 37521376

Gene position: 1926785-1925961 (Counterclockwise)

Preceding gene: 37521377

Following gene: 37521375

Centisome position: 41.36

GC content: 66.67

Gene sequence:

>825_bases
GTGATCCAGACCCAATCGCCGCCCGATGCGCTGCAGATTGCCAATCGCTCCTTCCGCTCGCGGCTGATGACCGGCACCGG
CAAATATCGCTCCTTCGAGGTGATGCGCGCCGCCGTCGCCGCTTCTGGAAGCGAAATTGTCACCGTCGCTGTGCGGCGGG
TGCAGGAGGGTGTGCCGGGCCACGGCGGCCTCGGCCAGGAACTCAATTGGCAAAAACTCTGGATGCTGCCCAACACCGCC
GGGGCGAAGAGCGCCGAAGAAGCGGTGCGCTACGCCCACTTCGGCCGCGAGATGGCGAAGATCCTGGGCCAGGAGGACAA
CCACTGGGTGAAGCTCGAGGTGATCCCCGAGACCAAGTACCTGCTGCCCGACCCCCTCGGCACCTTGCAGGCGGCGGAGA
CGCTCATCAAACAGGGCTTCACGGTGCTGCCCTATATCAACGCCGATTTTCACCTGGCCCGGCGGCTCTGGGAGATGGGC
TGCGCCACGGTGATGCCTTTGGGTTCTCCCATCGGCTCCGGCCAGGGCCTTCAAAACGCGGCGGCCATTGCGCTCATTAT
TGCCGAATCGCCGGTGCCGGTGGTGGTGGACGCGGGCATCCGCACACCTTCGGAGGCTTGCCGGGCGATGGAGATGGGAG
CGGCGGCGCTGCTGATCAACACCGCCATCGCCCAGGCCGAAGAGCCCATCGCCATGGGCCGTGCGATGGGCCTGGCCACC
GAGGCGGGCCGGCTTGCCCATCAGGCGGGGGCCATCCCGGTCAAAACCTACGCCAGCGCCAGTTCGCCGCTTACGGGACT
GGTGGGGAGCGCTGTCGGCAGTTGA

Upstream 100 bases:

>100_bases
CCCGCCTGCTGGTCGGCAAGCAGGTCTACTCGTTGGTGCAGGGAGAAGAGGCGCCGGCAAACGGGGACGACCACTATCAC
CCCGACACGGAGGATCTGCT

Downstream 100 bases:

>100_bases
AGCCCACGGATGCCGTCGGGTCGGGGGGGAACGCTGTGAAAGTCGTCGAAACCGTGGCCAGGCTCGAAGCGTTCCGGCGG
GCGCGCGCCCCGGTCGCCGG

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MIQTQSPPDALQIANRSFRSRLMTGTGKYRSFEVMRAAVAASGSEIVTVAVRRVQEGVPGHGGLGQELNWQKLWMLPNTA
GAKSAEEAVRYAHFGREMAKILGQEDNHWVKLEVIPETKYLLPDPLGTLQAAETLIKQGFTVLPYINADFHLARRLWEMG
CATVMPLGSPIGSGQGLQNAAAIALIIAESPVPVVVDAGIRTPSEACRAMEMGAAALLINTAIAQAEEPIAMGRAMGLAT
EAGRLAHQAGAIPVKTYASASSPLTGLVGSAVGS

Sequences:

>Translated_274_residues
MIQTQSPPDALQIANRSFRSRLMTGTGKYRSFEVMRAAVAASGSEIVTVAVRRVQEGVPGHGGLGQELNWQKLWMLPNTA
GAKSAEEAVRYAHFGREMAKILGQEDNHWVKLEVIPETKYLLPDPLGTLQAAETLIKQGFTVLPYINADFHLARRLWEMG
CATVMPLGSPIGSGQGLQNAAAIALIIAESPVPVVVDAGIRTPSEACRAMEMGAAALLINTAIAQAEEPIAMGRAMGLAT
EAGRLAHQAGAIPVKTYASASSPLTGLVGSAVGS
>Mature_274_residues
MIQTQSPPDALQIANRSFRSRLMTGTGKYRSFEVMRAAVAASGSEIVTVAVRRVQEGVPGHGGLGQELNWQKLWMLPNTA
GAKSAEEAVRYAHFGREMAKILGQEDNHWVKLEVIPETKYLLPDPLGTLQAAETLIKQGFTVLPYINADFHLARRLWEMG
CATVMPLGSPIGSGQGLQNAAAIALIIAESPVPVVVDAGIRTPSEACRAMEMGAAALLINTAIAQAEEPIAMGRAMGLAT
EAGRLAHQAGAIPVKTYASASSPLTGLVGSAVGS

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=258, Percent_Identity=51.5503875968992, Blast_Score=248, Evalue=3e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_GLOVI (Q7NJM5)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_924753.1
- ProteinModelPortal:   Q7NJM5
- SMR:   Q7NJM5
- GeneID:   2600209
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.1807
- HOGENOM:   HBG296821
- OMA:   PIIIDAG
- ProtClustDB:   PRK00208
- BioCyc:   GVIO251221:GLL1807-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 28895; Mature: 28895

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: ACT_SITE 111-111 BINDING 172-172

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQTQSPPDALQIANRSFRSRLMTGTGKYRSFEVMRAAVAASGSEIVTVAVRRVQEGVPG
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
HGGLGQELNWQKLWMLPNTAGAKSAEEAVRYAHFGREMAKILGQEDNHWVKLEVIPETKY
CCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
LLPDPLGTLQAAETLIKQGFTVLPYINADFHLARRLWEMGCATVMPLGSPIGSGQGLQNA
CCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCHHEECCCCCCCCCCCCCCC
AAIALIIAESPVPVVVDAGIRTPSEACRAMEMGAAALLINTAIAQAEEPIAMGRAMGLAT
EEEEEEEECCCCCEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHH
EAGRLAHQAGAIPVKTYASASSPLTGLVGSAVGS
HHHHHHHHCCCCCEEHHCCCCCCHHHHHHHHCCC
>Mature Secondary Structure
MIQTQSPPDALQIANRSFRSRLMTGTGKYRSFEVMRAAVAASGSEIVTVAVRRVQEGVPG
CCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCC
HGGLGQELNWQKLWMLPNTAGAKSAEEAVRYAHFGREMAKILGQEDNHWVKLEVIPETKY
CCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCC
LLPDPLGTLQAAETLIKQGFTVLPYINADFHLARRLWEMGCATVMPLGSPIGSGQGLQNA
CCCCCCHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCHHEECCCCCCCCCCCCCCC
AAIALIIAESPVPVVVDAGIRTPSEACRAMEMGAAALLINTAIAQAEEPIAMGRAMGLAT
EEEEEEEECCCCCEEEECCCCCHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHH
EAGRLAHQAGAIPVKTYASASSPLTGLVGSAVGS
HHHHHHHHCCCCCEEHHCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292