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The map label for this gene is rfbM [H]

Identifier: 37521351

GI number: 37521351

Start: 1896539

End: 1897522

Strand: Reverse

Name: rfbM [H]

Synonym: gvip246

Alternate gene names: 37521351

Gene position: 1897522-1896539 (Counterclockwise)

Preceding gene: 37521352

Following gene: 37521350

Centisome position: 40.73

GC content: 64.43

Gene sequence:

>984_bases
ATGAAAGCATTTGTGCTTGCCGCCGGCAAAGGCACCCGCCTGCGCCCCTTCACCGACGCCCTCCCTAAACCCCTGATGCC
CGTCATCAACAAACCGGTCATGGCCCACATCCTCGGGCTGTGCCGCAAGCACGGCTTCGACGAGATCGTCGCCAACCTCC
ACTACCGCGGTGAAAAAATCGTCGAGCGCTTCGCGGACGGACACGACCATGGTGTCTGCCTGCAGTACTCCTGGGAAGAA
CAGCTCCTCGGCACCGCCGGCGGCGTGCGCAGGCAGGCGGGCTTTTTGGCCGGCGGCCCCTTTTTGGTCATCTCTGGCGA
CGTGATGACCGACTTGGACCTGGGCGCCCTGGTGCGCTTCCACAAACAATCCGGCGCTGTCGCCACCATGGCGGTCAAAG
AAGTCGGCGACCCGAGCCGTTTCGGCGTCGTGGTCGCGGACCCCGACGGCCGTGTGCGCAGCTTTCAGGAAAAACCGGCC
AAAGGCACCGAGCGCTCGCGCCTGGCGAATACGGGCATCTACGTGTTGGAGCCGGAGGTGTTCGAGTACATCCCCGAGGC
GGCCTTTTTCGATTTCGGCAACGACCTGTTTCCGCTGCTGGTCTGCAAGGGGGCTCCGGTCTATGCGATGGGCACGGGGG
CGTACTGGTCGGATGTCGGGACGCTCTCGCAGTATCTGTACACGCACTGGGAGCTGTTGACCCATCCGGAGATCGCCCAG
CGCATCGGCGAAGGGACGGTGGTGCAAGCCGGGGCGGTGGTCTCCGAACAGGCCCTGATAGGCTCCGGCTGCACGATCGA
GAAGGGTGCGCGGGTGCTGGGGTATAGCTGCGTGAGCGACGGGTGCACGATCCGCGCGGACAGCGTGGTGGTGGACAGTG
TGGTGTGGACGAGCGAGCGGTGGGGACACAAGCTCAAAGGCGACCTGGTGCGTTCGATCTGGGGCGACGGGATGCAGGTC
TCACTCGGACTGCCCAATCTCTAA

Upstream 100 bases:

>100_bases
AAGACCGAGTACGGCCAGTACCTCAGAAACCTTGTCGAGAAGACAAACCGCTAGAACCCTCCCGCTTCGCAACACCCATT
GACTTTACAGGAGCAAAGCG

Downstream 100 bases:

>100_bases
GTACACCCACCAGGCATATAGATGAACCTGCGTCACAAAGCAATCAAGGGGGTAGGCTGGTCTGCCGTTCAAAATTTCGG
CAGCCAGTTCCTTTCCCTGC

Product: mannose-1-phosphate guanylyltransferase

Products: NA

Alternate protein names: UDP-N-acetylglucosamine pyrophosphorylase; N-acetylglucosamine-1-phosphate uridyltransferase; Glucosamine-1-phosphate N-acetyltransferase [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MKAFVLAAGKGTRLRPFTDALPKPLMPVINKPVMAHILGLCRKHGFDEIVANLHYRGEKIVERFADGHDHGVCLQYSWEE
QLLGTAGGVRRQAGFLAGGPFLVISGDVMTDLDLGALVRFHKQSGAVATMAVKEVGDPSRFGVVVADPDGRVRSFQEKPA
KGTERSRLANTGIYVLEPEVFEYIPEAAFFDFGNDLFPLLVCKGAPVYAMGTGAYWSDVGTLSQYLYTHWELLTHPEIAQ
RIGEGTVVQAGAVVSEQALIGSGCTIEKGARVLGYSCVSDGCTIRADSVVVDSVVWTSERWGHKLKGDLVRSIWGDGMQV
SLGLPNL

Sequences:

>Translated_327_residues
MKAFVLAAGKGTRLRPFTDALPKPLMPVINKPVMAHILGLCRKHGFDEIVANLHYRGEKIVERFADGHDHGVCLQYSWEE
QLLGTAGGVRRQAGFLAGGPFLVISGDVMTDLDLGALVRFHKQSGAVATMAVKEVGDPSRFGVVVADPDGRVRSFQEKPA
KGTERSRLANTGIYVLEPEVFEYIPEAAFFDFGNDLFPLLVCKGAPVYAMGTGAYWSDVGTLSQYLYTHWELLTHPEIAQ
RIGEGTVVQAGAVVSEQALIGSGCTIEKGARVLGYSCVSDGCTIRADSVVVDSVVWTSERWGHKLKGDLVRSIWGDGMQV
SLGLPNL
>Mature_327_residues
MKAFVLAAGKGTRLRPFTDALPKPLMPVINKPVMAHILGLCRKHGFDEIVANLHYRGEKIVERFADGHDHGVCLQYSWEE
QLLGTAGGVRRQAGFLAGGPFLVISGDVMTDLDLGALVRFHKQSGAVATMAVKEVGDPSRFGVVVADPDGRVRSFQEKPA
KGTERSRLANTGIYVLEPEVFEYIPEAAFFDFGNDLFPLLVCKGAPVYAMGTGAYWSDVGTLSQYLYTHWELLTHPEIAQ
RIGEGTVVQAGAVVSEQALIGSGCTIEKGARVLGYSCVSDGCTIRADSVVVDSVVWTSERWGHKLKGDLVRSIWGDGMQV
SLGLPNL

Specific function: Catalyzes the last two sequential reactions in the de novo biosynthetic pathway for UDP-N-acetyl-glucosamine (UDP- GlcNAc). Responsible for the acetylation of GlcN-1-P to GlcNAc-1- P, and for the uridyl transfer from UTP to GlcNAc-1-P, to produce UDP-GlcN

COG id: COG1208

COG function: function code MJ; Nucleoside-diphosphate-sugar pyrophosphorylase involved in lipopolysaccharide biosynthesis/translation initiation factor 2B, gamma/epsilon subunits (eIF-2Bgamma/eIF-2Bepsilon)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transferase hexapeptide repeat family [H]

Homologues:

Organism=Homo sapiens, GI11761621, Length=308, Percent_Identity=31.4935064935065, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI11761619, Length=308, Percent_Identity=31.4935064935065, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI31881779, Length=356, Percent_Identity=28.0898876404494, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI45447090, Length=356, Percent_Identity=28.0898876404494, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1790224, Length=230, Percent_Identity=30, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1788351, Length=229, Percent_Identity=30.5676855895196, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1789837, Length=346, Percent_Identity=26.5895953757225, Blast_Score=66, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI133931050, Length=303, Percent_Identity=32.6732673267327, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17509979, Length=330, Percent_Identity=31.2121212121212, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17509981, Length=319, Percent_Identity=30.4075235109718, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320148, Length=343, Percent_Identity=30.0291545189504, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI21355443, Length=305, Percent_Identity=31.1475409836066, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24644084, Length=305, Percent_Identity=31.1475409836066, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24653912, Length=361, Percent_Identity=26.5927977839335, Blast_Score=82, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005835
- InterPro:   IPR011004 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.23; =2.3.1.157 [H]

Molecular weight: Translated: 35407; Mature: 35407

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAFVLAAGKGTRLRPFTDALPKPLMPVINKPVMAHILGLCRKHGFDEIVANLHYRGEKI
CCEEEEECCCCCCCCCHHHHCCCCCHHHHCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHH
VERFADGHDHGVCLQYSWEEQLLGTAGGVRRQAGFLAGGPFLVISGDVMTDLDLGALVRF
HHHHHCCCCCCEEEEECHHHHHHHCCCCHHHHCCEEECCCEEEEECCHHCCCCHHHHHHH
HKQSGAVATMAVKEVGDPSRFGVVVADPDGRVRSFQEKPAKGTERSRLANTGIYVLEPEV
HHCCCCEEEEEHHHCCCCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHHCCEEEECCHH
FEYIPEAAFFDFGNDLFPLLVCKGAPVYAMGTGAYWSDVGTLSQYLYTHWELLTHPEIAQ
HHHCCCHHHHCCCCCCEEHEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHH
RIGEGTVVQAGAVVSEQALIGSGCTIEKGARVLGYSCVSDGCTIRADSVVVDSVVWTSER
HCCCCCEEECCCHHHHHHHCCCCCCHHCCCCEEEEHHHCCCCEEEECCEEEEHHEECCHH
WGHKLKGDLVRSIWGDGMQVSLGLPNL
CCCHHHHHHHHHHCCCCEEEEECCCCC
>Mature Secondary Structure
MKAFVLAAGKGTRLRPFTDALPKPLMPVINKPVMAHILGLCRKHGFDEIVANLHYRGEKI
CCEEEEECCCCCCCCCHHHHCCCCCHHHHCCHHHHHHHHHHHHCCHHHHHHHHHCCHHHH
VERFADGHDHGVCLQYSWEEQLLGTAGGVRRQAGFLAGGPFLVISGDVMTDLDLGALVRF
HHHHHCCCCCCEEEEECHHHHHHHCCCCHHHHCCEEECCCEEEEECCHHCCCCHHHHHHH
HKQSGAVATMAVKEVGDPSRFGVVVADPDGRVRSFQEKPAKGTERSRLANTGIYVLEPEV
HHCCCCEEEEEHHHCCCCCCEEEEEECCCCHHHHHHHCCCCCCHHHHHHHCCEEEECCHH
FEYIPEAAFFDFGNDLFPLLVCKGAPVYAMGTGAYWSDVGTLSQYLYTHWELLTHPEIAQ
HHHCCCHHHHCCCCCCEEHEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHH
RIGEGTVVQAGAVVSEQALIGSGCTIEKGARVLGYSCVSDGCTIRADSVVVDSVVWTSER
HCCCCCEEECCCHHHHHHHCCCCCCHHCCCCEEEEHHHCCCCEEEECCEEEEHHEECCHH
WGHKLKGDLVRSIWGDGMQVSLGLPNL
CCCHHHHHHHHHHCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]