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The map label for this gene is glpK

Identifier: 37521320

GI number: 37521320

Start: 1857081

End: 1858574

Strand: Reverse

Name: glpK

Synonym: gvip242

Alternate gene names: 37521320

Gene position: 1858574-1857081 (Counterclockwise)

Preceding gene: 37521321

Following gene: 37521319

Centisome position: 39.89

GC content: 67.87

Gene sequence:

>1494_bases
GTGGTTTTTGCCTCCGCGGGTGCTGCTGTGAGCGGGGCGATTCTGGCCCTCGATCTGGGGACGACGGGCATCCGGGCGCT
GCTGTTCGACCCGTCCGGGGCGGTGGCGGCAGGAGCCTACCGGGAAGTGCCGCAGATCTACCCGCAGCCGGGCTGGGTCG
AGCACGACCCGCAGACAATCTGGCAGCTTACCTGCGAAGTAGTGGCCGAGGTCCAGGCGCAAAGTGCAGCGCGCATCGCC
GCGGTCGGCCTCACCAACCAGCGCGAGACGTGCCTGTTGTGGGACGCGGCCACCGGCACCCCCCACGGCAACGCGATCGT
CTGGCAGGACCGCCGCACCGCCGCACTTTGCCAGAAATTGCGCGCCGAAGGCTGGGAAGCCCCTATCCGGCAGCGCACCG
GTCTGGTGATCGACGCCTATTTTTCGGCCACCAAACTGGCCTGGTTGCTGGCCCACCGCCGGCCCTACTATCCCGGCCTC
AAAGCGGGCACCATCGACAGCTGGATTATCTGGAAGCTCACCGGCGGGCGGGTGCACGCCACCGACACCAGTAACGCCTC
GCGCACGATGCTGTTCAATTTGCACACCCGCGACTGGGATCCGGAGCTGTTGGAGCTTCTGGCCATCCCGGCGGAAATTT
TGCCTGCGATCAAGCCTTCCCTCGGGGTACTCGCCGAGACCGACGTGCGGGTTTTGGGTTACAGTGCGCCGATCGCGGGC
ATTCTGGGCGATCAGCAGGCGGCTCTGTTTGCCCACGGCTGCGACCGGCCGGGCCTGGTCAAATGCACCTACGGCACGGG
CTCTTTTCTGGTGGCCCACACGGGCGACCGCCCCATCCGCTCGCGCCACCAACTGTTGACTACCGTCGCCTGGAGCGACC
GCACCTCCACCGGCTACGCGCTGGAGGGGGCGCTGTTTACGACCGGGGCGAGCGTGCAGTGGCTGCGCGACGGCCTCGGG
ATCATCGAGACTGCCGATGAGAGTGAGGCGCTTGCCGCGAGCGTCCCCGACAGCGGCGGAGTCTATTTTGTCCCCGCCCT
GAGTGGTCTGGGGGCTCCCCACTGGGATATGGGGGCGCGGGGGTTGCTGATTGGCCTCACGCGCGGCAGCGGGCGGGGCC
AGATAGCAAGGGCGGTGCTCGAAGCCATCGCCTTTCAGACTCGGGAGGTGACCGACGCCCTCGCCGCCGACATGGGCACG
CCCCTCACCCGGCTCAAAGTGGACGGCGGTGCGGTGCGCAACAACTTGCTGATGCAGTTGCAGGCGGACGTGCTCGGGGT
GCCGGTCGAACGGCCGCAGCTCATCGACACCACCGCCCAGGGCGCCGCCTTTGCCGCCGGCTTGGGGACCGGCTTCTGGG
GCGACTACGCCGAATTGGTAGCTGCCCGTCCCATCGACCGCGTCTTCGAATCGGGCGAACGGCAACTTGTTCTGCAGGCG
CACTACGCAGTCTGGCAGCGGGCGGTCGAGCGCTCGCGCGAGTGGGTGCGTTAG

Upstream 100 bases:

>100_bases
AGACTTTGCTTTGGGGGAGCTTCCGGCAGGGGTTCGACATCAAAAAGGCGGGGGTGGTGCGCATCGACCTGCTCCATCCC
CAGGACCAGGGGCGGTTGGT

Downstream 100 bases:

>100_bases
TAAGAAACCTTCCCCCGCAGGAGGGAGACGCCTGGCCACCTTTGTCTATCCTGGTGCTCTCTCCCGTCGCGCAGTGAGCA
CCTACAGTGGACCTCGAAAC

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK

Number of amino acids: Translated: 497; Mature: 497

Protein sequence:

>497_residues
MVFASAGAAVSGAILALDLGTTGIRALLFDPSGAVAAGAYREVPQIYPQPGWVEHDPQTIWQLTCEVVAEVQAQSAARIA
AVGLTNQRETCLLWDAATGTPHGNAIVWQDRRTAALCQKLRAEGWEAPIRQRTGLVIDAYFSATKLAWLLAHRRPYYPGL
KAGTIDSWIIWKLTGGRVHATDTSNASRTMLFNLHTRDWDPELLELLAIPAEILPAIKPSLGVLAETDVRVLGYSAPIAG
ILGDQQAALFAHGCDRPGLVKCTYGTGSFLVAHTGDRPIRSRHQLLTTVAWSDRTSTGYALEGALFTTGASVQWLRDGLG
IIETADESEALAASVPDSGGVYFVPALSGLGAPHWDMGARGLLIGLTRGSGRGQIARAVLEAIAFQTREVTDALAADMGT
PLTRLKVDGGAVRNNLLMQLQADVLGVPVERPQLIDTTAQGAAFAAGLGTGFWGDYAELVAARPIDRVFESGERQLVLQA
HYAVWQRAVERSREWVR

Sequences:

>Translated_497_residues
MVFASAGAAVSGAILALDLGTTGIRALLFDPSGAVAAGAYREVPQIYPQPGWVEHDPQTIWQLTCEVVAEVQAQSAARIA
AVGLTNQRETCLLWDAATGTPHGNAIVWQDRRTAALCQKLRAEGWEAPIRQRTGLVIDAYFSATKLAWLLAHRRPYYPGL
KAGTIDSWIIWKLTGGRVHATDTSNASRTMLFNLHTRDWDPELLELLAIPAEILPAIKPSLGVLAETDVRVLGYSAPIAG
ILGDQQAALFAHGCDRPGLVKCTYGTGSFLVAHTGDRPIRSRHQLLTTVAWSDRTSTGYALEGALFTTGASVQWLRDGLG
IIETADESEALAASVPDSGGVYFVPALSGLGAPHWDMGARGLLIGLTRGSGRGQIARAVLEAIAFQTREVTDALAADMGT
PLTRLKVDGGAVRNNLLMQLQADVLGVPVERPQLIDTTAQGAAFAAGLGTGFWGDYAELVAARPIDRVFESGERQLVLQA
HYAVWQRAVERSREWVR
>Mature_497_residues
MVFASAGAAVSGAILALDLGTTGIRALLFDPSGAVAAGAYREVPQIYPQPGWVEHDPQTIWQLTCEVVAEVQAQSAARIA
AVGLTNQRETCLLWDAATGTPHGNAIVWQDRRTAALCQKLRAEGWEAPIRQRTGLVIDAYFSATKLAWLLAHRRPYYPGL
KAGTIDSWIIWKLTGGRVHATDTSNASRTMLFNLHTRDWDPELLELLAIPAEILPAIKPSLGVLAETDVRVLGYSAPIAG
ILGDQQAALFAHGCDRPGLVKCTYGTGSFLVAHTGDRPIRSRHQLLTTVAWSDRTSTGYALEGALFTTGASVQWLRDGLG
IIETADESEALAASVPDSGGVYFVPALSGLGAPHWDMGARGLLIGLTRGSGRGQIARAVLEAIAFQTREVTDALAADMGT
PLTRLKVDGGAVRNNLLMQLQADVLGVPVERPQLIDTTAQGAAFAAGLGTGFWGDYAELVAARPIDRVFESGERQLVLQA
HYAVWQRAVERSREWVR

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family

Homologues:

Organism=Homo sapiens, GI189409120, Length=518, Percent_Identity=43.050193050193, Blast_Score=383, Evalue=1e-106,
Organism=Homo sapiens, GI4504007, Length=518, Percent_Identity=43.050193050193, Blast_Score=382, Evalue=1e-106,
Organism=Homo sapiens, GI42794763, Length=523, Percent_Identity=42.82982791587, Blast_Score=380, Evalue=1e-105,
Organism=Homo sapiens, GI41393575, Length=515, Percent_Identity=42.3300970873786, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI88196792, Length=508, Percent_Identity=31.6929133858268, Blast_Score=277, Evalue=2e-74,
Organism=Escherichia coli, GI1790361, Length=491, Percent_Identity=50.3054989816701, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI1790005, Length=457, Percent_Identity=26.2582056892779, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1787789, Length=482, Percent_Identity=28.2157676348548, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789987, Length=421, Percent_Identity=25.41567695962, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1789138, Length=463, Percent_Identity=23.9740820734341, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789168, Length=461, Percent_Identity=23.4273318872017, Blast_Score=74, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17535599, Length=442, Percent_Identity=41.8552036199095, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI71986140, Length=516, Percent_Identity=26.3565891472868, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI71986147, Length=296, Percent_Identity=30.4054054054054, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321755, Length=553, Percent_Identity=33.6347197106691, Blast_Score=275, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24655555, Length=515, Percent_Identity=40.5825242718447, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655560, Length=515, Percent_Identity=40.5825242718447, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655570, Length=515, Percent_Identity=40.5825242718447, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655574, Length=515, Percent_Identity=40.5825242718447, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24655565, Length=515, Percent_Identity=40.5825242718447, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI17864214, Length=512, Percent_Identity=37.3046875, Blast_Score=317, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24654573, Length=443, Percent_Identity=39.0519187358916, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI19922058, Length=539, Percent_Identity=30.7977736549165, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24652871, Length=503, Percent_Identity=30.6163021868787, Blast_Score=219, Evalue=5e-57,
Organism=Drosophila melanogaster, GI24656367, Length=508, Percent_Identity=30.1181102362205, Blast_Score=218, Evalue=9e-57,
Organism=Drosophila melanogaster, GI21357867, Length=530, Percent_Identity=29.0566037735849, Blast_Score=213, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24656372, Length=350, Percent_Identity=31.7142857142857, Blast_Score=166, Evalue=3e-41,
Organism=Drosophila melanogaster, GI45552909, Length=291, Percent_Identity=29.553264604811, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GLPK_GLOVI (Q7NJT1)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_924697.1
- ProteinModelPortal:   Q7NJT1
- SMR:   Q7NJT1
- GeneID:   2600153
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.1751
- HOGENOM:   HBG511469
- OMA:   KPSSEVY
- BioCyc:   GVIO251221:GLL1751-MONOMER
- BRENDA:   2.7.1.30
- HAMAP:   MF_00186
- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999
- PANTHER:   PTHR10196
- PANTHER:   PTHR10196:SF9
- TIGRFAMs:   TIGR01311

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N

EC number: =2.7.1.30

Molecular weight: Translated: 53311; Mature: 53311

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS00933 FGGY_KINASES_1; PS00445 FGGY_KINASES_2

Important sites: BINDING 21-21 BINDING 25-25 BINDING 88-88 BINDING 140-140 BINDING 244-244 BINDING 266-266 BINDING 309-309

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFASAGAAVSGAILALDLGTTGIRALLFDPSGAVAAGAYREVPQIYPQPGWVEHDPQTI
CEEECCCCCCCCEEEEEECCCCCEEEEEECCCCCEECCHHHHCHHHCCCCCCCCCCHHHH
WQLTCEVVAEVQAQSAARIAAVGLTNQRETCLLWDAATGTPHGNAIVWQDRRTAALCQKL
HHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHH
RAEGWEAPIRQRTGLVIDAYFSATKLAWLLAHRRPYYPGLKAGTIDSWIIWKLTGGRVHA
HHCCCCCCHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCEEEE
TDTSNASRTMLFNLHTRDWDPELLELLAIPAEILPAIKPSLGVLAETDVRVLGYSAPIAG
CCCCCCCEEEEEEEECCCCCHHHHHHHHCCHHHHHHCCCCCCEEECCCEEEEEECCCCHH
ILGDQQAALFAHGCDRPGLVKCTYGTGSFLVAHTGDRPIRSRHQLLTTVAWSDRTSTGYA
HCCCCCHHHEECCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHEEEEECCCCCCCEE
LEGALFTTGASVQWLRDGLGIIETADESEALAASVPDSGGVYFVPALSGLGAPHWDMGAR
EECEEEECCCCHHHHHCCCCEEEECCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCC
GLLIGLTRGSGRGQIARAVLEAIAFQTREVTDALAADMGTPLTRLKVDGGAVRNNLLMQL
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHEEE
QADVLGVPVERPQLIDTTAQGAAFAAGLGTGFWGDYAELVAARPIDRVFESGERQLVLQA
EHHHHCCCCCCCCEECCCCCCCEEEHHCCCCCCHHHHHHHHCCCHHHHHHCCCCEEEEHH
HYAVWQRAVERSREWVR
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVFASAGAAVSGAILALDLGTTGIRALLFDPSGAVAAGAYREVPQIYPQPGWVEHDPQTI
CEEECCCCCCCCEEEEEECCCCCEEEEEECCCCCEECCHHHHCHHHCCCCCCCCCCHHHH
WQLTCEVVAEVQAQSAARIAAVGLTNQRETCLLWDAATGTPHGNAIVWQDRRTAALCQKL
HHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCCEEEECCCCHHHHHHHH
RAEGWEAPIRQRTGLVIDAYFSATKLAWLLAHRRPYYPGLKAGTIDSWIIWKLTGGRVHA
HHCCCCCCHHHCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCEEEE
TDTSNASRTMLFNLHTRDWDPELLELLAIPAEILPAIKPSLGVLAETDVRVLGYSAPIAG
CCCCCCCEEEEEEEECCCCCHHHHHHHHCCHHHHHHCCCCCCEEECCCEEEEEECCCCHH
ILGDQQAALFAHGCDRPGLVKCTYGTGSFLVAHTGDRPIRSRHQLLTTVAWSDRTSTGYA
HCCCCCHHHEECCCCCCCEEEEEECCCCEEEEECCCCCHHHHHHHHEEEEECCCCCCCEE
LEGALFTTGASVQWLRDGLGIIETADESEALAASVPDSGGVYFVPALSGLGAPHWDMGAR
EECEEEECCCCHHHHHCCCCEEEECCCCCCEEEECCCCCCEEEEEECCCCCCCCCCCCCC
GLLIGLTRGSGRGQIARAVLEAIAFQTREVTDALAADMGTPLTRLKVDGGAVRNNLLMQL
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHEEE
QADVLGVPVERPQLIDTTAQGAAFAAGLGTGFWGDYAELVAARPIDRVFESGERQLVLQA
EHHHHCCCCCCCCEECCCCCCCEEEHHCCCCCCHHHHHHHHCCCHHHHHHCCCCEEEEHH
HYAVWQRAVERSREWVR
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292