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The map label for this gene is hup [H]

Identifier: 37520401

GI number: 37520401

Start: 888180

End: 888464

Strand: Reverse

Name: hup [H]

Synonym: gvip100

Alternate gene names: 37520401

Gene position: 888464-888180 (Counterclockwise)

Preceding gene: 37520407

Following gene: 37520395

Centisome position: 19.07

GC content: 55.09

Gene sequence:

>285_bases
ATGAACAAAGGCGAATTGGTGAAGGCTGTGGCCGAGCGAACCAAGATAACCCTCAAAGAAGCGGATGCCGTGATCAGCGC
CGTCTTCGACGAAATCCAGGACACCGTCGCCGGTGGCGAAAAAGTCACCCTGGTCGGATTTGGCACCTTCGAAGCGCGTA
AACGGGCCGAGCGGGAGGGACGCAACCCGAAGACCAATGAAAAAATGATCATTCCTGCGACGGTTGCACCGGCCTTCAGC
GCGGGCAAAACCTTCAAAGAAGCAGTGCTTACCAACAATAAGTAG

Upstream 100 bases:

>100_bases
TGTTATTGCGAATTATTGACGGCCAAAGCGCTCAAATCGGCTCTACTCAAGGCTCTCATGCTTTAATATTTCAAGACAGA
TCTGAGGAGACGTCGACTAC

Downstream 100 bases:

>100_bases
TAGATACCAGACTTCGAATAGGGATATGCAGGGGCATATCCCTATTTTTTGGGCGCTTGGGGCGCGGGAAGCGTGGGAGC
CGCCTGGGGAATGGCCGTCG

Product: DNA binding protein HU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 94; Mature: 94

Protein sequence:

>94_residues
MNKGELVKAVAERTKITLKEADAVISAVFDEIQDTVAGGEKVTLVGFGTFEARKRAEREGRNPKTNEKMIIPATVAPAFS
AGKTFKEAVLTNNK

Sequences:

>Translated_94_residues
MNKGELVKAVAERTKITLKEADAVISAVFDEIQDTVAGGEKVTLVGFGTFEARKRAEREGRNPKTNEKMIIPATVAPAFS
AGKTFKEAVLTNNK
>Mature_94_residues
MNKGELVKAVAERTKITLKEADAVISAVFDEIQDTVAGGEKVTLVGFGTFEARKRAEREGRNPKTNEKMIIPATVAPAFS
AGKTFKEAVLTNNK

Specific function: Histone-like DNA-binding protein which is capable of wrapping DNA to stabilize it, and thus to prevent its denaturation under extreme environmental conditions [H]

COG id: COG0776

COG function: function code L; Bacterial nucleoid DNA-binding protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial histone-like protein family [H]

Homologues:

Organism=Escherichia coli, GI1790433, Length=89, Percent_Identity=41.5730337078652, Blast_Score=77, Evalue=2e-16,
Organism=Escherichia coli, GI1786644, Length=89, Percent_Identity=39.3258426966292, Blast_Score=72, Evalue=8e-15,
Organism=Escherichia coli, GI1787141, Length=88, Percent_Identity=32.9545454545455, Blast_Score=61, Evalue=1e-11,
Organism=Escherichia coli, GI1788005, Length=89, Percent_Identity=32.5842696629214, Blast_Score=60, Evalue=3e-11,

Paralogues:

None

Copy number: 1380 Molecules/Cell In: Growth-Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000119
- InterPro:   IPR020816
- InterPro:   IPR010992 [H]

Pfam domain/function: PF00216 Bac_DNA_binding [H]

EC number: NA

Molecular weight: Translated: 10152; Mature: 10152

Theoretical pI: Translated: 10.15; Mature: 10.15

Prosite motif: PS00045 HISTONE_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKGELVKAVAERTKITLKEADAVISAVFDEIQDTVAGGEKVTLVGFGTFEARKRAEREG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCC
RNPKTNEKMIIPATVAPAFSAGKTFKEAVLTNNK
CCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MNKGELVKAVAERTKITLKEADAVISAVFDEIQDTVAGGEKVTLVGFGTFEARKRAEREG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHCC
RNPKTNEKMIIPATVAPAFSAGKTFKEAVLTNNK
CCCCCCCEEEEECCCCCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]