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The map label for this gene is pilJ [H]

Identifier: 34498314

GI number: 34498314

Start: 3116514

End: 3118130

Strand: Reverse

Name: pilJ [H]

Synonym: CV_2859

Alternate gene names: 34498314

Gene position: 3118130-3116514 (Counterclockwise)

Preceding gene: 34498316

Following gene: 34498313

Centisome position: 65.63

GC content: 65.0

Gene sequence:

>1617_bases
ATGACCATTACCAAGAGACTGCTGTTGACCACCGTGCTGGCGCTGCTGGCGATGCTGGCGCTGGGCGTGCAGGGTCTGTG
GAAACAGCAGCAGGCCAATGACCGCTTCGACTACATGAAGATCAACACCTTCCCCAGCATCCACACGCTGGACGACGCCA
AGGAGGCGCTGGCCGACGTGAGGCTGACGCTGGCGCGCAGCGTGGCGCTTTCCGATCCAGCTGCCAAGAAGGCGGAATTC
GCCGCGCTGGCGGACCTGGACAAGCGTTTCGACGCGGCGCTGGACAAGTACGAGAAAGATTTGGTTTCCAACGACGCCGA
CCGCAAGCTGCTGCAGACCGACCGCGAGGACATGAAGCTTTACCGCCAGCAGCGCGACAAATACCTGCAGCTGCTGCAGA
GCAACAATGTGGCCGAGGCGGACAAGCTGATGACCGGCGACATGTCCAACGAGGCCAAGGCGCTGAACCAGGCGCTGATC
GATCACATCGAATTCAACTACAAGCTGGCCGATCAATTGAGCAAGGACAATGCCGCCGCCTATACCGCCGCATGGTGGCA
GTCGGCGATACTGGTGGCGTCGGCCGTTCTGGTGTGCGGCTGGCTCAGCGGCCACCTGTTCCTCAACATCCGCAGCAGCC
TGGAGCAGACGCTGAGCGCGATGCAGGCGGTCCGGACGACGCTGGATTTCCGCCTGCGCGCGCCGGTGGCGCGGATGGAT
GAGATCGGCAAGACCGCCACCGCGTTCAACGAGCTGATGGCCAGGCTGCAGCAAAGCTTCAAGGAAATCCGCCACAGCGT
CGAAGAGATGGAGGGCGCCATCGTCCATATGGCGGAGTCTTCCGGCGACATCGCTTCCAGCTCGGTGCAGCAGAGCGAGT
CGGCATCGGCTATGGCGGCGGCGGTGGAGCAGGTGACGGTCAGCATCAATCACGTGGCGGACCGCGCGCGCGAGGCCAAC
CAGCAGGCGCGGGAGGCCGGCGAGATCGCCGACCGCGGCAGCAAGGTGGTGCTGGGCACGGTGGACGGCATCAGCGCGGT
GGCGGAGTCGGTGGGCGAAGCCTCCGAGCGCATCGGCAAGCTGCGGGAGGACAGCGTCACCATCGCCACCGTGCTGAGCG
TGATCAAGGATATCGCCGATCAAACCAACCTTTTGGCGCTGAACGCCGCGATCGAAGCCGCCCGGGCCGGCGAAATGGGC
CGCGGCTTCGCCGTGGTGGCCGACGAGGTGCGCAAGCTGGCCGAACGCACCGCCAGCTCCACCACCGAGATCTCCGCGGT
GATAGACAAGATGCAGCAGGGCACCCAGGCCGCGGTGAGCGGCATGCAGGAGGTGGTGACGCGGGTGAACGAGGAGGCGG
AAAAGGCGCGCGGCGCCAGCGACGCCATCCAGCAGATCAAGACCAATTCCGAGCACAGCATGCTGCTGATAGGCGAGATT
TCCGGCAGCATCGTCGAACAGGGCAGCGCCAGCAACACCATCGCGCAGAAGGTGGAGCAGATCGCGCAGATGGCGGAGGC
CAATTCGCGCGCCTCGTCCGACTCGGCCGGCTCCGCGCAGGAGCTGCGCAGCCAGGCCAGCAACATCCAGCGCACGGTGG
CGCAATACCAGGTGTGA

Upstream 100 bases:

>100_bases
ACGGAATGATTTGTTGTTTGTTTGACAAAGAAAGCTATAAATATTCTAAGTAAATTCTCGTAAGTCCAGTTTGACGCGCC
TGGCCTGCAAGGAGTTGGAC

Downstream 100 bases:

>100_bases
ACCAGACAACGACAGGGATCGGGGAAGATCCGACAGGGCCGCGAGGCCCTGTTTTCTTTGGGCGAGCGGTTTTGCAGGAA
CCGCGATAAGCAAGCCAGGA

Product: methyl-accepting chemotaxis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 537

Protein sequence:

>538_residues
MTITKRLLLTTVLALLAMLALGVQGLWKQQQANDRFDYMKINTFPSIHTLDDAKEALADVRLTLARSVALSDPAAKKAEF
AALADLDKRFDAALDKYEKDLVSNDADRKLLQTDREDMKLYRQQRDKYLQLLQSNNVAEADKLMTGDMSNEAKALNQALI
DHIEFNYKLADQLSKDNAAAYTAAWWQSAILVASAVLVCGWLSGHLFLNIRSSLEQTLSAMQAVRTTLDFRLRAPVARMD
EIGKTATAFNELMARLQQSFKEIRHSVEEMEGAIVHMAESSGDIASSSVQQSESASAMAAAVEQVTVSINHVADRAREAN
QQAREAGEIADRGSKVVLGTVDGISAVAESVGEASERIGKLREDSVTIATVLSVIKDIADQTNLLALNAAIEAARAGEMG
RGFAVVADEVRKLAERTASSTTEISAVIDKMQQGTQAAVSGMQEVVTRVNEEAEKARGASDAIQQIKTNSEHSMLLIGEI
SGSIVEQGSASNTIAQKVEQIAQMAEANSRASSDSAGSAQELRSQASNIQRTVAQYQV

Sequences:

>Translated_538_residues
MTITKRLLLTTVLALLAMLALGVQGLWKQQQANDRFDYMKINTFPSIHTLDDAKEALADVRLTLARSVALSDPAAKKAEF
AALADLDKRFDAALDKYEKDLVSNDADRKLLQTDREDMKLYRQQRDKYLQLLQSNNVAEADKLMTGDMSNEAKALNQALI
DHIEFNYKLADQLSKDNAAAYTAAWWQSAILVASAVLVCGWLSGHLFLNIRSSLEQTLSAMQAVRTTLDFRLRAPVARMD
EIGKTATAFNELMARLQQSFKEIRHSVEEMEGAIVHMAESSGDIASSSVQQSESASAMAAAVEQVTVSINHVADRAREAN
QQAREAGEIADRGSKVVLGTVDGISAVAESVGEASERIGKLREDSVTIATVLSVIKDIADQTNLLALNAAIEAARAGEMG
RGFAVVADEVRKLAERTASSTTEISAVIDKMQQGTQAAVSGMQEVVTRVNEEAEKARGASDAIQQIKTNSEHSMLLIGEI
SGSIVEQGSASNTIAQKVEQIAQMAEANSRASSDSAGSAQELRSQASNIQRTVAQYQV
>Mature_537_residues
TITKRLLLTTVLALLAMLALGVQGLWKQQQANDRFDYMKINTFPSIHTLDDAKEALADVRLTLARSVALSDPAAKKAEFA
ALADLDKRFDAALDKYEKDLVSNDADRKLLQTDREDMKLYRQQRDKYLQLLQSNNVAEADKLMTGDMSNEAKALNQALID
HIEFNYKLADQLSKDNAAAYTAAWWQSAILVASAVLVCGWLSGHLFLNIRSSLEQTLSAMQAVRTTLDFRLRAPVARMDE
IGKTATAFNELMARLQQSFKEIRHSVEEMEGAIVHMAESSGDIASSSVQQSESASAMAAAVEQVTVSINHVADRAREANQ
QAREAGEIADRGSKVVLGTVDGISAVAESVGEASERIGKLREDSVTIATVLSVIKDIADQTNLLALNAAIEAARAGEMGR
GFAVVADEVRKLAERTASSTTEISAVIDKMQQGTQAAVSGMQEVVTRVNEEAEKARGASDAIQQIKTNSEHSMLLIGEIS
GSIVEQGSASNTIAQKVEQIAQMAEANSRASSDSAGSAQELRSQASNIQRTVAQYQV

Specific function: May be a part of a signal-transduction system that regulates twitching motility by controlling pilus function (extension and retraction) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 methyl-accepting transducer domain [H]

Homologues:

Organism=Escherichia coli, GI2367378, Length=445, Percent_Identity=29.2134831460674, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1788194, Length=314, Percent_Identity=31.2101910828025, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788195, Length=269, Percent_Identity=32.7137546468402, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787690, Length=196, Percent_Identity=36.2244897959184, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1789453, Length=296, Percent_Identity=28.7162162162162, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004090
- InterPro:   IPR004089
- InterPro:   IPR003660 [H]

Pfam domain/function: PF00015 MCPsignal [H]

EC number: NA

Molecular weight: Translated: 58236; Mature: 58105

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50885 HAMP ; PS50111 CHEMOTAXIS_TRANSDUC_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTITKRLLLTTVLALLAMLALGVQGLWKQQQANDRFDYMKINTFPSIHTLDDAKEALADV
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHH
RLTLARSVALSDPAAKKAEFAALADLDKRFDAALDKYEKDLVSNDADRKLLQTDREDMKL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHH
YRQQRDKYLQLLQSNNVAEADKLMTGDMSNEAKALNQALIDHIEFNYKLADQLSKDNAAA
HHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHH
YTAAWWQSAILVASAVLVCGWLSGHLFLNIRSSLEQTLSAMQAVRTTLDFRLRAPVARMD
HHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
EIGKTATAFNELMARLQQSFKEIRHSVEEMEGAIVHMAESSGDIASSSVQQSESASAMAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHHHHHHHHH
AVEQVTVSINHVADRAREANQQAREAGEIADRGSKVVLGTVDGISAVAESVGEASERIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHH
LREDSVTIATVLSVIKDIADQTNLLALNAAIEAARAGEMGRGFAVVADEVRKLAERTASS
HHHCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
TTEISAVIDKMQQGTQAAVSGMQEVVTRVNEEAEKARGASDAIQQIKTNSEHSMLLIGEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECC
SGSIVEQGSASNTIAQKVEQIAQMAEANSRASSDSAGSAQELRSQASNIQRTVAQYQV
CCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TITKRLLLTTVLALLAMLALGVQGLWKQQQANDRFDYMKINTFPSIHTLDDAKEALADV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHH
RLTLARSVALSDPAAKKAEFAALADLDKRFDAALDKYEKDLVSNDADRKLLQTDREDMKL
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHH
YRQQRDKYLQLLQSNNVAEADKLMTGDMSNEAKALNQALIDHIEFNYKLADQLSKDNAAA
HHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCHH
YTAAWWQSAILVASAVLVCGWLSGHLFLNIRSSLEQTLSAMQAVRTTLDFRLRAPVARMD
HHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
EIGKTATAFNELMARLQQSFKEIRHSVEEMEGAIVHMAESSGDIASSSVQQSESASAMAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCCCCHHHHHHHHHHHHHHHHH
AVEQVTVSINHVADRAREANQQAREAGEIADRGSKVVLGTVDGISAVAESVGEASERIGK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHHHHHHH
LREDSVTIATVLSVIKDIADQTNLLALNAAIEAARAGEMGRGFAVVADEVRKLAERTASS
HHHCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
TTEISAVIDKMQQGTQAAVSGMQEVVTRVNEEAEKARGASDAIQQIKTNSEHSMLLIGEI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEECC
SGSIVEQGSASNTIAQKVEQIAQMAEANSRASSDSAGSAQELRSQASNIQRTVAQYQV
CCHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7908398; 10984043 [H]